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Protein

BTB/POZ and MATH domain-containing protein 3

Gene

BPM3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May act as a substrate-specific adapter of an E3 ubiquitin-protein ligase complex (CUL3-RBX1-BTB) which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • identical protein binding Source: UniProtKB

GO - Biological processi

  • cellular response to salt stress Source: TAIR
  • protein ubiquitination Source: UniProtKB-UniPathway
  • regulation of protein ubiquitination Source: TAIR
  • response to osmotic stress Source: TAIR
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-ATH-5632684. Hedgehog 'on' state.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
BTB/POZ and MATH domain-containing protein 3
Alternative name(s):
Protein BTB-POZ AND MATH DOMAIN 3
Short name:
AtBPM3
Gene namesi
Name:BPM3
Ordered Locus Names:At2g39760
ORF Names:T5I7.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G39760.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication

GO - Cellular componenti

  • cytosol Source: TAIR
  • nucleus Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 408408BTB/POZ and MATH domain-containing protein 3PRO_0000405267Add
BLAST

Proteomic databases

PaxDbiO22286.
PRIDEiO22286.

Expressioni

Tissue specificityi

Ubiquitous.2 Publications

Gene expression databases

GenevisibleiO22286. AT.

Interactioni

Subunit structurei

Homodimer or heterodimer with BPM3 and BPM5. Interacts with CUL3A and CUL3B. Interacts with RAP2-4 and RAP2-13.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
CUL3AQ9ZVH43EBI-540923,EBI-531362
RAP2-4Q8H1E42EBI-540923,EBI-7528315

GO - Molecular functioni

  • identical protein binding Source: UniProtKB

Protein-protein interaction databases

BioGridi3899. 34 interactions.
IntActiO22286. 28 interactions.
MINTiMINT-7262735.
STRINGi3702.AT2G39760.1.

Structurei

3D structure databases

ProteinModelPortaliO22286.
SMRiO22286. Positions 21-359.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 158135MATHPROSITE-ProRule annotationAdd
BLAST
Domaini194 – 26168BTBPROSITE-ProRule annotationAdd
BLAST

Domaini

The BTB/POZ domain mediates the interaction with some component of ubiquitin ligase complexes.

Sequence similaritiesi

Belongs to the Tdpoz family.Curated
Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 1 MATH domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1987. Eukaryota.
ENOG410XQV8. LUCA.
HOGENOMiHOG000220921.
InParanoidiO22286.
KOiK10523.
OMAiVGCDIAF.
PhylomeDBiO22286.

Family and domain databases

Gene3Di2.60.210.10. 1 hit.
InterProiIPR000210. BTB/POZ_dom.
IPR002083. MATH/TRAF_dom.
IPR011333. SKP1/BTB/POZ.
IPR008974. TRAF-like.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
PF00917. MATH. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00061. MATH. 1 hit.
[Graphical view]
SUPFAMiSSF49599. SSF49599. 1 hit.
SSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS50144. MATH. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O22286-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSTVGGIEQL IPDSVSTSFI ETVNGSHQFT IQGYSLAKGM SPGKFIQSDI
60 70 80 90 100
FSVGGYDWAI YFYPDGKNPE DQSSYISLFI ALASDSNDIR ALFELTLMDQ
110 120 130 140 150
SGKGKHKVHS HFDRALEGGP YTLKYKGSMW GYKRFFKRSA LETSDYLKDD
160 170 180 190 200
CLVINCTVGV VRARLEGPKQ YGIVLPLSNM GQGLKDLLDS EVGCDIAFQV
210 220 230 240 250
GDETYKAHKL ILAARSPVFR AQFFGPIGNN NVDRIVIDDI EPSIFKAMLS
260 270 280 290 300
FIYTDVLPNV HEITGSTSAS SFTNMIQHLL AAADLYDLAR LKILCEVLLC
310 320 330 340 350
EKLDVDNVAT TLALAEQHQF LQLKAFCLEF VASPANLGAV MKSEGFKHLK
360 370 380 390 400
QSCPTLLSEL LNTVAAADKS STSGQSNKKR SASSVLGCDT TNVRQLRRRT

RKEVRAVS
Length:408
Mass (Da):44,890
Last modified:January 1, 1998 - v1
Checksum:i31B93225F439926B
GO
Isoform 2 (identifier: O22286-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     339-343: AVMKS → GTGCI
     344-408: Missing.

Note: May be due to intron retention. No experimental confirmation available.
Show »
Length:343
Mass (Da):37,719
Checksum:i35139EB100555D5E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti329 – 3291E → K in AAM66127 (Ref. 5) Curated
Sequence conflicti396 – 3961L → F in AAM66127 (Ref. 5) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei339 – 3435AVMKS → GTGCI in isoform 2. 1 PublicationVSP_040655
Alternative sequencei344 – 40865Missing in isoform 2. 1 PublicationVSP_040656Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003000 Genomic DNA. Translation: AAB87125.1.
CP002685 Genomic DNA. Translation: AEC09719.1.
CP002685 Genomic DNA. Translation: AEC09720.1.
AY128315 mRNA. Translation: AAM91518.1.
BT000044 mRNA. Translation: AAN15363.1.
AK316691 mRNA. Translation: BAH19418.1.
AY088598 mRNA. Translation: AAM66127.1.
PIRiT01006.
RefSeqiNP_001031516.1. NM_001036439.1. [O22286-2]
NP_030522.1. NM_129534.3. [O22286-1]
UniGeneiAt.37134.

Genome annotation databases

EnsemblPlantsiAT2G39760.1; AT2G39760.1; AT2G39760. [O22286-1]
GeneIDi818561.
KEGGiath:AT2G39760.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantsUBQ

A functional genomics database for the ubiquitin/26S proteasome proteolytic pathway in plants

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC003000 Genomic DNA. Translation: AAB87125.1.
CP002685 Genomic DNA. Translation: AEC09719.1.
CP002685 Genomic DNA. Translation: AEC09720.1.
AY128315 mRNA. Translation: AAM91518.1.
BT000044 mRNA. Translation: AAN15363.1.
AK316691 mRNA. Translation: BAH19418.1.
AY088598 mRNA. Translation: AAM66127.1.
PIRiT01006.
RefSeqiNP_001031516.1. NM_001036439.1. [O22286-2]
NP_030522.1. NM_129534.3. [O22286-1]
UniGeneiAt.37134.

3D structure databases

ProteinModelPortaliO22286.
SMRiO22286. Positions 21-359.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi3899. 34 interactions.
IntActiO22286. 28 interactions.
MINTiMINT-7262735.
STRINGi3702.AT2G39760.1.

Proteomic databases

PaxDbiO22286.
PRIDEiO22286.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G39760.1; AT2G39760.1; AT2G39760. [O22286-1]
GeneIDi818561.
KEGGiath:AT2G39760.

Organism-specific databases

TAIRiAT2G39760.

Phylogenomic databases

eggNOGiKOG1987. Eukaryota.
ENOG410XQV8. LUCA.
HOGENOMiHOG000220921.
InParanoidiO22286.
KOiK10523.
OMAiVGCDIAF.
PhylomeDBiO22286.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-ATH-5632684. Hedgehog 'on' state.

Miscellaneous databases

PROiO22286.

Gene expression databases

GenevisibleiO22286. AT.

Family and domain databases

Gene3Di2.60.210.10. 1 hit.
InterProiIPR000210. BTB/POZ_dom.
IPR002083. MATH/TRAF_dom.
IPR011333. SKP1/BTB/POZ.
IPR008974. TRAF-like.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
PF00917. MATH. 1 hit.
[Graphical view]
SMARTiSM00225. BTB. 1 hit.
SM00061. MATH. 1 hit.
[Graphical view]
SUPFAMiSSF49599. SSF49599. 1 hit.
SSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS50144. MATH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  4. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Strain: cv. Columbia.
    Tissue: Rosette leaf.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  6. "TDPOZ, a family of bipartite animal and plant proteins that contain the TRAF (TD) and POZ/BTB domains."
    Huang C.-J., Chen C.-Y., Chen H.-H., Tsai S.-F., Choo K.-B.
    Gene 324:117-127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY.
  7. "Arabidopsis has two redundant Cullin3 proteins that are essential for embryo development and that interact with RBX1 and BTB proteins to form multisubunit E3 ubiquitin ligase complexes in vivo."
    Figueroa P., Gusmaroli G., Serino G., Habashi J., Ma L., Shen Y., Feng S., Bostick M., Callis J., Hellmann H., Deng X.W.
    Plant Cell 17:1180-1195(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CUL3A.
  8. "Arabidopsis AtCUL3a and AtCUL3b form complexes with members of the BTB/POZ-MATH protein family."
    Weber H., Bernhardt A., Dieterle M., Hano P., Mutlu A., Estelle M., Genschik P., Hellmann H.
    Plant Physiol. 137:83-93(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE, FUNCTION, IDENTIFICATION IN THE CUL3-RBX1-BTB UBIQUITIN-PROTEIN LIGASE COMPLEX, INTERACTION WITH CUL3A AND CUL3B, SUBUNIT, TISSUE SPECIFICITY.
  9. "Arabidopsis thaliana BTB/ POZ-MATH proteins interact with members of the ERF/AP2 transcription factor family."
    Weber H., Hellmann H.
    FEBS J. 276:6624-6635(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAP2-4 AND RAP2-13, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiBPM3_ARATH
AccessioniPrimary (citable) accession number: O22286
Secondary accession number(s): Q2V416, Q8L977
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 8, 2011
Last sequence update: January 1, 1998
Last modified: January 20, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.