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Protein

Histidine kinase CKI1

Gene

CKI1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential protein. Functions as a histidine kinase and transmits the stress signal to a downstream MAPK cascade. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain. Required for the development of megagametophyte in female gametophyte (embryo sac) independently of cytokinin. Contributes to vascular bundle formation and secondary growth in a cytokinin-independent manner, probably by promoting the maintenance of mitotic activity and/or identity of procambial cells. Seems to influence and promote the cytokinin signaling pathway.7 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • phosphorelay sensor kinase activity Source: InterPro
  • protein histidine kinase activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

  • cytokinin-activated signaling pathway Source: TAIR
  • embryo sac development Source: TAIR
  • peptidyl-histidine phosphorylation Source: GOC
  • phloem or xylem histogenesis Source: UniProtKB
  • secondary growth Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Transferase

Enzyme and pathway databases

BioCyciARA:AT2G47430-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine kinase CKI1 (EC:2.7.13.3)
Alternative name(s):
Protein CYTOKININ-INDEPENDENT 1
Gene namesi
Name:CKI1
Ordered Locus Names:At2g47430
ORF Names:T30B22.27
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G47430.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence analysisAdd
BLAST
Transmembranei13 – 3321HelicalSequence analysisAdd
BLAST
Topological domaini34 – 345312ExtracellularSequence analysisAdd
BLAST
Transmembranei346 – 36621HelicalSequence analysisAdd
BLAST
Topological domaini367 – 1122756CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: UniProtKB
  • plasmodesma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Can not be transmitted through the female germ line. Impaired megagametogenesis visible from the four-nucleate stage, with two normal nuclei and two degenerated nuclei, leading to female sterility. Normal response to cytokinins.3 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi150 – 1534RGDY → GT in cki1-r; normal phenotype rescued from En-1 insertional disruption phenotype. 1 Publication
Mutagenesisi405 – 4051H → Q: Dysfunction of the two-component signaling pathway leading to defects in procambial cell maintenance and proliferation, as well as the absence of secondary growth. 1 Publication
Mutagenesisi1050 – 10501D → Q or E: Loss of histidine kinase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11221122Histidine kinase CKI1PRO_0000398591Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei405 – 4051Phosphohistidine; by autocatalysisPROSITE-ProRule annotation
Modified residuei1050 – 105014-aspartylphosphatePROSITE-ProRule annotation

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO22267.
PRIDEiO22267.

Expressioni

Tissue specificityi

Expressed in vascular tissues of inflorescence stems and floral organs, especially in procambium cells, and in siliques.3 Publications

Developmental stagei

Present at low levels in developing ovules. Within mature female gametophytes, high levels in the central cell nucleus and weak levels in the egg cell nucleus. In fertilized ovules, expressed in the endosperm nuclei during 2 days after pollination.2 Publications

Gene expression databases

GenevisibleiO22267. AT.

Interactioni

Subunit structurei

Homodimer. Interacts with AHP2 and AHP3.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi4691. 5 interactions.
IntActiO22267. 3 interactions.
STRINGi3702.AT2G47430.1.

Structurei

Secondary structure

1
1122
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni980 – 9856Combined sources
Beta strandi987 – 9915Combined sources
Helixi995 – 100713Combined sources
Beta strandi1011 – 10188Combined sources
Helixi1019 – 103618Combined sources
Beta strandi1045 – 10517Combined sources
Beta strandi1054 – 10563Combined sources
Helixi1058 – 107013Combined sources
Turni1071 – 10733Combined sources
Beta strandi1078 – 10847Combined sources
Helixi1088 – 109710Combined sources
Beta strandi1100 – 11045Combined sources
Helixi1110 – 11178Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MM4X-ray2.00A944-1122[»]
3MMNX-ray2.20A944-1122[»]
ProteinModelPortaliO22267.
SMRiO22267. Positions 370-669, 979-1118.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini402 – 671270Histidine kinasePROSITE-ProRule annotationAdd
BLAST
Domaini987 – 1120134Response regulatoryPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IRDA. Eukaryota.
ENOG410XT1K. LUCA.
HOGENOMiHOG000114067.
InParanoidiO22267.
OMAiMDMINIV.
PhylomeDBiO22267.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 2 hits.
InterProiIPR011006. CheY-like_superfamily.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55874. SSF55874. 2 hits.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O22267-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMVKVTKLVA SRPIVVFCVL AFLVVVFECI WISNWRTTTE NLVKEVASFT
60 70 80 90 100
EDLRTSLVSE IENIGKFTYA KTNLSTIGLA RVIDSYITNN DTGFTEIQTQ
110 120 130 140 150
IAPLLFVAYS TILQVSQVSY ISRDGLMFSY IAESNTSVAV FANSSSNSSR
160 170 180 190 200
GDYTWYTQTV DQLTGRLNGN STKSQSLDVT HTDWFQAAQS NNYTTAFVGT
210 220 230 240 250
SLGGEDNETL IQSVVSLYSK KGLVSLGFPV KTLTEVLNSL NLHGEELYMW
260 270 280 290 300
TKDGTVLVRE GSLNDSFFIS NGSICFGRES NSLWSQCIPE NCSSSGYEVE
310 320 330 340 350
IKRLRYQAFC SVIEVSGVPL RYTLMFPNKG GATRIKHQAE KAKYQLIVVM
360 370 380 390 400
IFLGFGWPVW FVWFMMQATR REMHMRATLI NQMEATQQAE RKSMNKSQAF
410 420 430 440 450
ANASHDIRGA LAGMKGLIDI CRDGVKPGSD VDTTLNQVNV CAKDLVALLN
460 470 480 490 500
SVLDMSKIES GKMQLVEEDF NLSKLLEDVI DFYHPVAMKK GVDVVLDPHD
510 520 530 540 550
GSVFKFSNVR GDSGRLKQIL NNLVSNAVKF TVDGHIAVRA WAQRPGSNSS
560 570 580 590 600
VVLASYPKGV SKFVKSMFCK NKEESSTYET EISNSIRNNA NTMEFVFEVD
610 620 630 640 650
DTGKGIPMEM RKSVFENYVQ VRETAQGHQG TGLGLGIVQS LVRLMGGEIR
660 670 680 690 700
ITDKAMGEKG TCFQFNVLLT TLESPPVSDM KVRQEIEAGG DYVSTPNLGL
710 720 730 740 750
TINTSLGGSM NIRNLSPRFN NCLSSSPKQE GSRVVLLLKN EERRRVTEKY
760 770 780 790 800
IKNLGIKVTV VEKWEHLSYA LERLFGFSPQ SSMGRAECSL SCPSSRELPF
810 820 830 840 850
IGMDGIDSRS QLPKRRSISF SAVVLLVIDA KTGPFFELCD IVKQFRRGLP
860 870 880 890 900
HGISCKVVWL NESSTRVSER GDISCSRPLH GSRLMEVLKM LPEFGGTVLK
910 920 930 940 950
EPPTELQRES LLRHSFVAER SPKHKVQEEG PSSMFNKKLG KRIMASTDSE
960 970 980 990 1000
SETRVKSVRT GRKPIGNPED EQETSKPSDD EFLRGKRVLV VDDNFISRKV
1010 1020 1030 1040 1050
ATGKLKKMGV SEVEQCDSGK EALRLVTEGL TQREEQGSVD KLPFDYIFMD
1060 1070 1080 1090 1100
CQMPEMDGYE ATREIRKVEK SYGVRTPIIA VSGHDPGSEE ARETIQAGMD
1110 1120
AFLDKSLNQL ANVIREIESK RH
Length:1,122
Mass (Da):125,033
Last modified:January 1, 1998 - v1
Checksum:iC6833F731DA84781
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87545 mRNA. Translation: BAA13416.1.
AC002535 Genomic DNA. Translation: AAC62867.1.
CP002685 Genomic DNA. Translation: AEC10840.1.
EF598269 Genomic DNA. Translation: ABQ85241.1.
EF598270 Genomic DNA. Translation: ABQ85242.1.
EF598271 Genomic DNA. Translation: ABQ85243.1.
EF598272 Genomic DNA. Translation: ABQ85244.1.
EF598273 Genomic DNA. Translation: ABQ85245.1.
EF598274 Genomic DNA. Translation: ABQ85246.1.
EF598275 Genomic DNA. Translation: ABQ85247.1.
EF598276 Genomic DNA. Translation: ABQ85248.1.
EF598277 Genomic DNA. Translation: ABQ85249.1.
EF598278 Genomic DNA. Translation: ABQ85250.1.
EF598279 Genomic DNA. Translation: ABQ85251.1.
EF598280 Genomic DNA. Translation: ABQ85252.1.
EF598281 Genomic DNA. Translation: ABQ85253.1.
EF598282 Genomic DNA. Translation: ABQ85254.1.
EF598283 Genomic DNA. Translation: ABQ85255.1.
EF598284 Genomic DNA. Translation: ABQ85256.1.
EF598285 Genomic DNA. Translation: ABQ85257.1.
EF598286 Genomic DNA. Translation: ABQ85258.1.
EF598287 Genomic DNA. Translation: ABQ85259.1.
EF598288 Genomic DNA. Translation: ABQ85260.1.
EF598289 Genomic DNA. Translation: ABQ85261.1.
EF598290 Genomic DNA. Translation: ABQ85262.1.
EF598291 Genomic DNA. Translation: ABQ85263.1.
PIRiT00441.
RefSeqiNP_182265.1. NM_130311.2.
UniGeneiAt.341.

Genome annotation databases

EnsemblPlantsiAT2G47430.1; AT2G47430.1; AT2G47430.
GeneIDi819356.
GrameneiAT2G47430.1; AT2G47430.1; AT2G47430.
KEGGiath:AT2G47430.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87545 mRNA. Translation: BAA13416.1.
AC002535 Genomic DNA. Translation: AAC62867.1.
CP002685 Genomic DNA. Translation: AEC10840.1.
EF598269 Genomic DNA. Translation: ABQ85241.1.
EF598270 Genomic DNA. Translation: ABQ85242.1.
EF598271 Genomic DNA. Translation: ABQ85243.1.
EF598272 Genomic DNA. Translation: ABQ85244.1.
EF598273 Genomic DNA. Translation: ABQ85245.1.
EF598274 Genomic DNA. Translation: ABQ85246.1.
EF598275 Genomic DNA. Translation: ABQ85247.1.
EF598276 Genomic DNA. Translation: ABQ85248.1.
EF598277 Genomic DNA. Translation: ABQ85249.1.
EF598278 Genomic DNA. Translation: ABQ85250.1.
EF598279 Genomic DNA. Translation: ABQ85251.1.
EF598280 Genomic DNA. Translation: ABQ85252.1.
EF598281 Genomic DNA. Translation: ABQ85253.1.
EF598282 Genomic DNA. Translation: ABQ85254.1.
EF598283 Genomic DNA. Translation: ABQ85255.1.
EF598284 Genomic DNA. Translation: ABQ85256.1.
EF598285 Genomic DNA. Translation: ABQ85257.1.
EF598286 Genomic DNA. Translation: ABQ85258.1.
EF598287 Genomic DNA. Translation: ABQ85259.1.
EF598288 Genomic DNA. Translation: ABQ85260.1.
EF598289 Genomic DNA. Translation: ABQ85261.1.
EF598290 Genomic DNA. Translation: ABQ85262.1.
EF598291 Genomic DNA. Translation: ABQ85263.1.
PIRiT00441.
RefSeqiNP_182265.1. NM_130311.2.
UniGeneiAt.341.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3MM4X-ray2.00A944-1122[»]
3MMNX-ray2.20A944-1122[»]
ProteinModelPortaliO22267.
SMRiO22267. Positions 370-669, 979-1118.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4691. 5 interactions.
IntActiO22267. 3 interactions.
STRINGi3702.AT2G47430.1.

Proteomic databases

PaxDbiO22267.
PRIDEiO22267.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G47430.1; AT2G47430.1; AT2G47430.
GeneIDi819356.
GrameneiAT2G47430.1; AT2G47430.1; AT2G47430.
KEGGiath:AT2G47430.

Organism-specific databases

TAIRiAT2G47430.

Phylogenomic databases

eggNOGiENOG410IRDA. Eukaryota.
ENOG410XT1K. LUCA.
HOGENOMiHOG000114067.
InParanoidiO22267.
OMAiMDMINIV.
PhylomeDBiO22267.

Enzyme and pathway databases

BioCyciARA:AT2G47430-MONOMER.

Miscellaneous databases

PROiO22267.

Gene expression databases

GenevisibleiO22267. AT.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 2 hits.
InterProiIPR011006. CheY-like_superfamily.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55874. SSF55874. 2 hits.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "CKI1, a histidine kinase homolog implicated in cytokinin signal transduction."
    Kakimoto T.
    Science 274:982-985(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: cv. Wassilewskija.
    Tissue: Shoot.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "The genetic architecture of shoot branching in Arabidopsis thaliana: a comparative assessment of candidate gene associations vs. quantitative trait locus mapping."
    Ehrenreich I.M., Stafford P.A., Purugganan M.D.
    Genetics 176:1223-1236(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 740-1003.
    Strain: cv. Ag-0, cv. An-1, cv. Br-0, cv. C24, cv. Ct-1, cv. Cvi-1, cv. Edi-0, cv. Ga-0, cv. Kas-2, cv. Kin-0, cv. Landsberg erecta, cv. Lz-0, cv. Ms-0, cv. Mt-0, cv. Nd-1, cv. Nok-3, cv. Oy-0, cv. Se-0, cv. Sorbo, cv. Tsu-1, cv. Van-0, cv. Wa-1 and cv. Wassilewskija.
  5. "Biochemical characterization of a putative cytokinin-responsive His-kinase, CKI1, from Arabidopsis thaliana."
    Nakamura A., Kakimoto T., Imamura A., Suzuki T., Ueguchi C., Mizuno T.
    Biosci. Biotechnol. Biochem. 63:1627-1630(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ASP-1050.
  6. "Possible His to Asp phosphorelay signaling in an Arabidopsis two-component system."
    Urao T., Miyata S., Yamaguchi-Shinozaki K., Shinozaki K.
    FEBS Lett. 478:227-232(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AHP2 AND AHP3.
  7. Cited for: FUNCTION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
  8. "The putative sensor histidine kinase CKI1 is involved in female gametophyte development in Arabidopsis."
    Hejatko J., Pernisova M., Eneva T., Palme K., Brzobohaty B.
    Mol. Genet. Genomics 269:443-453(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, MUTAGENESIS OF 150-ARG--TYR-153, DEVELOPMENTAL STAGE.
  9. "Functional analysis of AHK1/ATHK1 and cytokinin receptor histidine kinases in response to abscisic acid, drought, and salt stress in Arabidopsis."
    Tran L.S., Urao T., Qin F., Maruyama K., Kakimoto T., Shinozaki K., Yamaguchi-Shinozaki K.
    Proc. Natl. Acad. Sci. U.S.A. 104:20623-20628(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "The histidine kinases CYTOKININ-INDEPENDENT1 and ARABIDOPSIS HISTIDINE KINASE2 and 3 regulate vascular tissue development in Arabidopsis shoots."
    Hejatko J., Ryu H., Kim G.-T., Dobesova R., Choi S., Choi S.M., Soucek P., Horak J., Pekarova B., Palme K., Brzobohaty B., Hwang I.
    Plant Cell 21:2008-2021(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, HOMODIMERIZATION, MUTAGENESIS OF HIS-405.
  11. "Arabidopsis histidine kinase CKI1 acts upstream of histidine phosphotransfer proteins to regulate female gametophyte development and vegetative growth."
    Deng Y., Dong H., Mu J., Ren B., Zheng B., Ji Z., Yang W.-C., Liang Y., Zuo J.
    Plant Cell 22:1232-1248(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY.

Entry informationi

Entry nameiCKI1_ARATH
AccessioniPrimary (citable) accession number: O22267
Secondary accession number(s): A5YY37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: January 1, 1998
Last modified: February 17, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.