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Protein

Histidine kinase CKI1

Gene

CKI1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential protein. Functions as a histidine kinase and transmits the stress signal to a downstream MAPK cascade. This protein undergoes an ATP-dependent autophosphorylation at a conserved histidine residue in the kinase core, and a phosphoryl group is then transferred to a conserved aspartate residue in the receiver domain. Required for the development of megagametophyte in female gametophyte (embryo sac) independently of cytokinin. Contributes to vascular bundle formation and secondary growth in a cytokinin-independent manner, probably by promoting the maintenance of mitotic activity and/or identity of procambial cells. Seems to influence and promote the cytokinin signaling pathway.7 Publications

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

  • phosphorelay sensor kinase activity Source: InterPro
  • protein histidine kinase activity Source: UniProtKB
  • protein homodimerization activity Source: UniProtKB

GO - Biological processi

  • cytokinin-activated signaling pathway Source: TAIR
  • embryo sac development Source: TAIR
  • nucleoside metabolic process Source: InterPro
  • phloem or xylem histogenesis Source: UniProtKB
  • secondary growth Source: UniProtKB

Keywordsi

Molecular functionDevelopmental protein, Kinase, Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Histidine kinase CKI1 (EC:2.7.13.3)
Alternative name(s):
Protein CYTOKININ-INDEPENDENT 1
Gene namesi
Name:CKI1
Ordered Locus Names:At2g47430
ORF Names:T30B22.27
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G47430
TAIRilocus:2061971 AT2G47430

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 33HelicalSequence analysisAdd BLAST21
Topological domaini34 – 345ExtracellularSequence analysisAdd BLAST312
Transmembranei346 – 366HelicalSequence analysisAdd BLAST21
Topological domaini367 – 1122CytoplasmicSequence analysisAdd BLAST756

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cannot be transmitted through the female germ line. Impaired megagametogenesis visible from the four-nucleate stage, with two normal nuclei and two degenerated nuclei, leading to female sterility. Normal response to cytokinins.3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi150 – 153RGDY → GT in cki1-r; normal phenotype rescued from En-1 insertional disruption phenotype. 1 Publication4
Mutagenesisi405H → Q: Dysfunction of the two-component signaling pathway leading to defects in procambial cell maintenance and proliferation, as well as the absence of secondary growth. 1 Publication1
Mutagenesisi1050D → Q or E: Loss of histidine kinase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003985911 – 1122Histidine kinase CKI1Add BLAST1122

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei405Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1
Modified residuei10504-aspartylphosphatePROSITE-ProRule annotation1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO22267
PRIDEiO22267

Expressioni

Tissue specificityi

Expressed in vascular tissues of inflorescence stems and floral organs, especially in procambium cells, and in siliques.3 Publications

Developmental stagei

Present at low levels in developing ovules. Within mature female gametophytes, high levels in the central cell nucleus and weak levels in the egg cell nucleus. In fertilized ovules, expressed in the endosperm nuclei during 2 days after pollination.2 Publications

Gene expression databases

ExpressionAtlasiO22267 differential
GenevisibleiO22267 AT

Interactioni

Subunit structurei

Homodimer. Interacts with AHP2 and AHP3.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi4691, 5 interactors
IntActiO22267, 3 interactors
STRINGi3702.AT2G47430.1

Structurei

Secondary structure

11122
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni980 – 985Combined sources6
Beta strandi987 – 991Combined sources5
Helixi995 – 1007Combined sources13
Beta strandi1011 – 1018Combined sources8
Helixi1019 – 1036Combined sources18
Beta strandi1045 – 1051Combined sources7
Beta strandi1054 – 1056Combined sources3
Helixi1058 – 1070Combined sources13
Turni1071 – 1073Combined sources3
Beta strandi1078 – 1084Combined sources7
Helixi1088 – 1097Combined sources10
Beta strandi1101 – 1104Combined sources4
Helixi1110 – 1119Combined sources10

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3MM4X-ray2.00A944-1122[»]
3MMNX-ray2.20A944-1122[»]
5LNMX-ray1.95A944-1122[»]
5LNNX-ray1.60A944-1122[»]
5N2NX-ray2.05A944-1122[»]
ProteinModelPortaliO22267
SMRiO22267
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini402 – 671Histidine kinasePROSITE-ProRule annotationAdd BLAST270
Domaini987 – 1120Response regulatoryPROSITE-ProRule annotationAdd BLAST134

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IRDA Eukaryota
ENOG410XT1K LUCA
HOGENOMiHOG000114067
InParanoidiO22267
OMAiFHAYSTI
OrthoDBiEOG093604SL
PhylomeDBiO22267

Family and domain databases

CDDicd00075 HATPase_c, 1 hit
cd00082 HisKA, 1 hit
cd06223 PRTases_typeI, 1 hit
cd00156 REC, 1 hit
InterProiView protein in InterPro
IPR011006 CheY-like_superfamily
IPR003594 HATPase_C
IPR036890 HATPase_C_sf
IPR005467 His_kinase_dom
IPR003661 HisK_dim/P
IPR036097 HisK_dim/P_sf
IPR000836 PRibTrfase_dom
IPR004358 Sig_transdc_His_kin-like_C
IPR001789 Sig_transdc_resp-reg_receiver
PfamiView protein in Pfam
PF02518 HATPase_c, 1 hit
PF00512 HisKA, 1 hit
PF00072 Response_reg, 1 hit
PRINTSiPR00344 BCTRLSENSOR
SMARTiView protein in SMART
SM00387 HATPase_c, 1 hit
SM00388 HisKA, 1 hit
SM00448 REC, 1 hit
SUPFAMiSSF47384 SSF47384, 1 hit
SSF52172 SSF52172, 1 hit
SSF55874 SSF55874, 2 hits
PROSITEiView protein in PROSITE
PS50109 HIS_KIN, 1 hit
PS50110 RESPONSE_REGULATORY, 1 hit

Sequencei

Sequence statusi: Complete.

O22267-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMVKVTKLVA SRPIVVFCVL AFLVVVFECI WISNWRTTTE NLVKEVASFT
60 70 80 90 100
EDLRTSLVSE IENIGKFTYA KTNLSTIGLA RVIDSYITNN DTGFTEIQTQ
110 120 130 140 150
IAPLLFVAYS TILQVSQVSY ISRDGLMFSY IAESNTSVAV FANSSSNSSR
160 170 180 190 200
GDYTWYTQTV DQLTGRLNGN STKSQSLDVT HTDWFQAAQS NNYTTAFVGT
210 220 230 240 250
SLGGEDNETL IQSVVSLYSK KGLVSLGFPV KTLTEVLNSL NLHGEELYMW
260 270 280 290 300
TKDGTVLVRE GSLNDSFFIS NGSICFGRES NSLWSQCIPE NCSSSGYEVE
310 320 330 340 350
IKRLRYQAFC SVIEVSGVPL RYTLMFPNKG GATRIKHQAE KAKYQLIVVM
360 370 380 390 400
IFLGFGWPVW FVWFMMQATR REMHMRATLI NQMEATQQAE RKSMNKSQAF
410 420 430 440 450
ANASHDIRGA LAGMKGLIDI CRDGVKPGSD VDTTLNQVNV CAKDLVALLN
460 470 480 490 500
SVLDMSKIES GKMQLVEEDF NLSKLLEDVI DFYHPVAMKK GVDVVLDPHD
510 520 530 540 550
GSVFKFSNVR GDSGRLKQIL NNLVSNAVKF TVDGHIAVRA WAQRPGSNSS
560 570 580 590 600
VVLASYPKGV SKFVKSMFCK NKEESSTYET EISNSIRNNA NTMEFVFEVD
610 620 630 640 650
DTGKGIPMEM RKSVFENYVQ VRETAQGHQG TGLGLGIVQS LVRLMGGEIR
660 670 680 690 700
ITDKAMGEKG TCFQFNVLLT TLESPPVSDM KVRQEIEAGG DYVSTPNLGL
710 720 730 740 750
TINTSLGGSM NIRNLSPRFN NCLSSSPKQE GSRVVLLLKN EERRRVTEKY
760 770 780 790 800
IKNLGIKVTV VEKWEHLSYA LERLFGFSPQ SSMGRAECSL SCPSSRELPF
810 820 830 840 850
IGMDGIDSRS QLPKRRSISF SAVVLLVIDA KTGPFFELCD IVKQFRRGLP
860 870 880 890 900
HGISCKVVWL NESSTRVSER GDISCSRPLH GSRLMEVLKM LPEFGGTVLK
910 920 930 940 950
EPPTELQRES LLRHSFVAER SPKHKVQEEG PSSMFNKKLG KRIMASTDSE
960 970 980 990 1000
SETRVKSVRT GRKPIGNPED EQETSKPSDD EFLRGKRVLV VDDNFISRKV
1010 1020 1030 1040 1050
ATGKLKKMGV SEVEQCDSGK EALRLVTEGL TQREEQGSVD KLPFDYIFMD
1060 1070 1080 1090 1100
CQMPEMDGYE ATREIRKVEK SYGVRTPIIA VSGHDPGSEE ARETIQAGMD
1110 1120
AFLDKSLNQL ANVIREIESK RH
Length:1,122
Mass (Da):125,033
Last modified:January 1, 1998 - v1
Checksum:iC6833F731DA84781
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87545 mRNA Translation: BAA13416.1
AC002535 Genomic DNA Translation: AAC62867.1
CP002685 Genomic DNA Translation: AEC10840.1
EF598269 Genomic DNA Translation: ABQ85241.1
EF598270 Genomic DNA Translation: ABQ85242.1
EF598271 Genomic DNA Translation: ABQ85243.1
EF598272 Genomic DNA Translation: ABQ85244.1
EF598273 Genomic DNA Translation: ABQ85245.1
EF598274 Genomic DNA Translation: ABQ85246.1
EF598275 Genomic DNA Translation: ABQ85247.1
EF598276 Genomic DNA Translation: ABQ85248.1
EF598277 Genomic DNA Translation: ABQ85249.1
EF598278 Genomic DNA Translation: ABQ85250.1
EF598279 Genomic DNA Translation: ABQ85251.1
EF598280 Genomic DNA Translation: ABQ85252.1
EF598281 Genomic DNA Translation: ABQ85253.1
EF598282 Genomic DNA Translation: ABQ85254.1
EF598283 Genomic DNA Translation: ABQ85255.1
EF598284 Genomic DNA Translation: ABQ85256.1
EF598285 Genomic DNA Translation: ABQ85257.1
EF598286 Genomic DNA Translation: ABQ85258.1
EF598287 Genomic DNA Translation: ABQ85259.1
EF598288 Genomic DNA Translation: ABQ85260.1
EF598289 Genomic DNA Translation: ABQ85261.1
EF598290 Genomic DNA Translation: ABQ85262.1
EF598291 Genomic DNA Translation: ABQ85263.1
PIRiT00441
RefSeqiNP_182265.1, NM_130311.2
UniGeneiAt.341

Genome annotation databases

EnsemblPlantsiAT2G47430.1; AT2G47430.1; AT2G47430
GeneIDi819356
GrameneiAT2G47430.1; AT2G47430.1; AT2G47430
KEGGiath:AT2G47430

Similar proteinsi

Entry informationi

Entry nameiCKI1_ARATH
AccessioniPrimary (citable) accession number: O22267
Secondary accession number(s): A5YY37
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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