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Protein

Protein disulfide-isomerase like 2-1

Gene

PDIL2-1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Protein disulfide isomerase that may be required for proper pollen development, ovule fertilization and embryo development.1 Publication

Catalytic activityi

Catalyzes the rearrangement of -S-S- bonds in proteins.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei52 – 521NucleophileBy similarity
Sitei53 – 531Contributes to redox potential valueBy similarity
Sitei54 – 541Contributes to redox potential valueBy similarity
Active sitei55 – 551NucleophileBy similarity
Sitei117 – 1171Lowers pKa of C-terminal Cys of first active siteBy similarity
Active sitei171 – 1711NucleophileBy similarity
Sitei172 – 1721Contributes to redox potential valueBy similarity
Sitei173 – 1731Contributes to redox potential valueBy similarity
Active sitei174 – 1741NucleophileBy similarity
Sitei236 – 2361Lowers pKa of C-terminal Cys of second active siteBy similarity

GO - Molecular functioni

GO - Biological processi

  • cell redox homeostasis Source: InterPro
  • double fertilization forming a zygote and endosperm Source: TAIR
  • embryo development ending in seed dormancy Source: TAIR
  • embryo sac development Source: TAIR
  • pollen tube development Source: TAIR
  • protein folding Source: GO_Central
  • response to cadmium ion Source: TAIR
  • response to endoplasmic reticulum stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Enzyme and pathway databases

BioCyciARA:AT2G47470-MONOMER.
ARA:GQT-263-MONOMER.
ARA:GQT-264-MONOMER.
ARA:GQT-265-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein disulfide-isomerase like 2-1 (EC:5.3.4.1)
Short name:
AtPDIL2-1
Alternative name(s):
P5
Protein MATERNAL EFFECT EMBRYO ARREST 30
Protein UNFERTILIZED EMBRYO SAC 5
Protein disulfide isomerase 11
Short name:
AtPDI11
Protein disulfide-isomerase A6
Protein disulfide-isomerase like 4-1
Short name:
AtPDIL4-1
Gene namesi
Name:PDIL2-1
Synonyms:MEE30, PDI11, PDIL4-1, UNE5
Ordered Locus Names:At2g47470
ORF Names:T30B22.23
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G47470.

Subcellular locationi

  • Endoplasmic reticulum 1 Publication

GO - Cellular componenti

  • endoplasmic reticulum Source: TAIR
  • plant-type cell wall Source: TAIR
  • vacuolar membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

Pathology & Biotechi

Disruption phenotypei

Smaller siliques and reduced seed set. Disrupted pollen tube guidance.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence analysisAdd
BLAST
Chaini23 – 361339Protein disulfide-isomerase like 2-1PRO_0000034242Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi52 ↔ 55Redox-activePROSITE-ProRule annotation
Disulfide bondi171 ↔ 174Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiO22263.
PRIDEiO22263.

2D gel databases

SWISS-2DPAGEO22263.

PTM databases

iPTMnetiO22263.
SwissPalmiO22263.

Expressioni

Tissue specificityi

Widely expressed.2 Publications

Developmental stagei

During flower development, expressed at early stage in inner and outer integuments, and nucellar cells. Later, expressed in the integument cells but not in the embryo sac. In the mature ovule, highly expressed in the micropylar region. After fertilization, expressed in the seed integuments but not in the embryo.1 Publication

Inductioni

By chemically-induced ER stress response.1 Publication

Gene expression databases

ExpressionAtlasiO22263. baseline and differential.
GenevisibleiO22263. AT.

Interactioni

Protein-protein interaction databases

BioGridi4695. 3 interactions.
IntActiO22263. 1 interaction.
STRINGi3702.AT2G47470.1.

Structurei

3D structure databases

ProteinModelPortaliO22263.
SMRiO22263. Positions 25-252, 264-357.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 131109Thioredoxin 1PROSITE-ProRule annotationAdd
BLAST
Domaini132 – 250119Thioredoxin 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein disulfide isomerase family.Curated
Contains 2 thioredoxin domains.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Repeat, Signal

Phylogenomic databases

eggNOGiKOG0191. Eukaryota.
COG0526. LUCA.
HOGENOMiHOG000176389.
InParanoidiO22263.
KOiK09584.
OMAiYKSGRDL.
PhylomeDBiO22263.

Family and domain databases

Gene3Di1.20.1150.12. 1 hit.
3.40.30.10. 2 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR011679. ER_p29_C.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF07749. ERp29. 1 hit.
PF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF47933. SSF47933. 1 hit.
SSF52833. SSF52833. 2 hits.
TIGRFAMsiTIGR01126. pdi_dom. 2 hits.
PROSITEiPS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: O22263-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAKSQIWFGF ALLALLLVSA VADDVVVLTD DSFEKEVGKD KGALVEFYAP
60 70 80 90 100
WCGHCKKLAP EYEKLGASFK KAKSVLIAKV DCDEQKSVCT KYGVSGYPTI
110 120 130 140 150
QWFPKGSLEP QKYEGPRNAE ALAEYVNKEG GTNVKLAAVP QNVVVLTPDN
160 170 180 190 200
FDEIVLDQNK DVLVEFYAPW CGHCKSLAPT YEKVATVFKQ EEGVVIANLD
210 220 230 240 250
ADAHKALGEK YGVSGFPTLK FFPKDNKAGH DYDGGRDLDD FVSFINEKSG
260 270 280 290 300
TSRDSKGQLT SKAGIVESLD ALVKELVAAS EDEKKAVLSR IEEEASTLKG
310 320 330 340 350
STTRYGKLYL KLAKSYIEKG SDYASKETER LGRVLGKSIS PVKADELTLK
360
RNILTTFVAS S
Length:361
Mass (Da):39,497
Last modified:January 1, 1998 - v1
Checksum:i9F4C7A07B0209DB5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083688 mRNA. Translation: AAN60247.1.
AJ271470 mRNA. Translation: CAC81060.1.
AC002535 Genomic DNA. Translation: AAC62863.1.
CP002685 Genomic DNA. Translation: AEC10845.1.
AY074348 mRNA. Translation: AAL67044.1.
AY091388 mRNA. Translation: AAM14327.1.
PIRiT00437.
RefSeqiNP_182269.1. NM_130315.4. [O22263-1]
UniGeneiAt.24396.
At.66351.

Genome annotation databases

EnsemblPlantsiAT2G47470.1; AT2G47470.1; AT2G47470. [O22263-1]
GeneIDi819360.
KEGGiath:AT2G47470.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF083688 mRNA. Translation: AAN60247.1.
AJ271470 mRNA. Translation: CAC81060.1.
AC002535 Genomic DNA. Translation: AAC62863.1.
CP002685 Genomic DNA. Translation: AEC10845.1.
AY074348 mRNA. Translation: AAL67044.1.
AY091388 mRNA. Translation: AAM14327.1.
PIRiT00437.
RefSeqiNP_182269.1. NM_130315.4. [O22263-1]
UniGeneiAt.24396.
At.66351.

3D structure databases

ProteinModelPortaliO22263.
SMRiO22263. Positions 25-252, 264-357.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4695. 3 interactions.
IntActiO22263. 1 interaction.
STRINGi3702.AT2G47470.1.

PTM databases

iPTMnetiO22263.
SwissPalmiO22263.

2D gel databases

SWISS-2DPAGEO22263.

Proteomic databases

PaxDbiO22263.
PRIDEiO22263.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G47470.1; AT2G47470.1; AT2G47470. [O22263-1]
GeneIDi819360.
KEGGiath:AT2G47470.

Organism-specific databases

TAIRiAT2G47470.

Phylogenomic databases

eggNOGiKOG0191. Eukaryota.
COG0526. LUCA.
HOGENOMiHOG000176389.
InParanoidiO22263.
KOiK09584.
OMAiYKSGRDL.
PhylomeDBiO22263.

Enzyme and pathway databases

BioCyciARA:AT2G47470-MONOMER.
ARA:GQT-263-MONOMER.
ARA:GQT-264-MONOMER.
ARA:GQT-265-MONOMER.

Miscellaneous databases

PROiO22263.

Gene expression databases

ExpressionAtlasiO22263. baseline and differential.
GenevisibleiO22263. AT.

Family and domain databases

Gene3Di1.20.1150.12. 1 hit.
3.40.30.10. 2 hits.
InterProiIPR005788. Disulphide_isomerase.
IPR011679. ER_p29_C.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF07749. ERp29. 1 hit.
PF00085. Thioredoxin. 2 hits.
[Graphical view]
SUPFAMiSSF47933. SSF47933. 1 hit.
SSF52833. SSF52833. 2 hits.
TIGRFAMsiTIGR01126. pdi_dom. 2 hits.
PROSITEiPS00194. THIOREDOXIN_1. 2 hits.
PS51352. THIOREDOXIN_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Signal peptide selection derived cDNAs from Arabidopsis thaliana leaves and guard cells."
    Stracke R., Palme K.
    Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Arabidopsis thaliana mRNA for ERp72."
    Mahon P.
    Thesis (2000), Cambridge University, United Kingdom
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Phylogenetic analyses identify 10 classes of the protein disulfide isomerase family in plants, including single-domain protein disulfide isomerase-related proteins."
    Houston N.L., Fan C., Xiang J.Q., Schulze J.M., Jung R., Boston R.S.
    Plant Physiol. 137:762-778(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  7. "Endoplasmic reticulum stress activates the expression of a sub-group of protein disulfide isomerase genes and AtbZIP60 modulates the response in Arabidopsis thaliana."
    Lu D.-P., Christopher D.A.
    Mol. Genet. Genomics 280:199-210(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INDUCTION.
  8. "Truncation of a protein disulfide isomerase, PDIL2-1, delays embryo sac maturation and disrupts pollen tube guidance in Arabidopsis thaliana."
    Wang H., Boavida L.C., Ron M., McCormick S.
    Plant Cell 20:3300-3311(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  9. "The protein disulfide isomerase gene family in bread wheat (T. aestivum L.)."
    d'Aloisio E., Paolacci A.R., Dhanapal A.P., Tanzarella O.A., Porceddu E., Ciaffi M.
    BMC Plant Biol. 10:101-101(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.

Entry informationi

Entry nameiPDI21_ARATH
AccessioniPrimary (citable) accession number: O22263
Secondary accession number(s): Q546R3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 1, 1998
Last modified: June 8, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.