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Protein

Nicotinamide adenine dinucleotide transporter 1, chloroplastic

Gene

NDT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the NAD+ import into chloroplast. Favors the NAD+(in)/ADP or AMP(out) antiport exchange, but is also able to catalyze a low unidirectional transport (uniport) of NAD+. Transports NAD+, nicotinic acid adenine dinucleotide, nicotinamide mononucleotide, nicotinic acid mononucleotide, FAD, FMN, TTP, TDP, TMP, UTP, UDP, UMP, CTP, CDP, CMP, GTP, GDP, GMP, 3'-AMP, ATP, ADP, AND AMP, has low transport activity with cAMP, pyrophosphate, NADH and alpha-NAD+, and has no activity with NADP+, NADPH, nicotinamide, nicotinic acid, adenosine, thiamine mono- or diphosphate, inorganic phosphate, CoA, folate, NaCl, malate, malonate, citrate, fumarate, aspartate, glutamate, S-adenosylmethionine, lysine, arginine, and ornithine.2 Publications

Enzyme regulationi

Inhibited by pyridoxal 5'-phosphate, bathophenanthroline, tannic acid, mersalyl, mercuric chloride, p-hydroxymercuribenzoate, p-hydroxymercuribenzoate sulfonate, bromocresol purple and N-ethylmaleimide.1 Publication

Kineticsi

  1. KM=0.24 mM for for the NAD+/NAD+ exchange1 Publication
  1. Vmax=1.41 mmol/min/g enzyme for the NAD+/NAD+ exchange1 Publication

GO - Molecular functioni

  • antiporter activity Source: UniProtKB-KW
  • NAD transporter activity Source: TAIR
  • structural constituent of ribosome Source: GO_Central

GO - Biological processi

  • NAD transport Source: TAIR
  • translation Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Antiport, Transport

Protein family/group databases

TCDBi2.A.29.10.11. the mitochondrial carrier (mc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinamide adenine dinucleotide transporter 1, chloroplastic
Short name:
AtNDT1
Alternative name(s):
NAD(+) transporter 1
Gene namesi
Name:NDT1
Synonyms:NADT1
Ordered Locus Names:At2g47490
ORF Names:T30B22.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G47490.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei17 – 3721Helical; Name=1Sequence analysisAdd
BLAST
Transmembranei78 – 9821Helical; Name=2Sequence analysisAdd
BLAST
Transmembranei117 – 13721Helical; Name=3Sequence analysisAdd
BLAST
Transmembranei171 – 19121Helical; Name=4Sequence analysisAdd
BLAST
Transmembranei216 – 23217Helical; Name=5Sequence analysisAdd
BLAST
Transmembranei271 – 29323Helical; Name=6Sequence analysisAdd
BLAST

GO - Cellular componenti

  • chloroplast membrane Source: TAIR
  • integral component of membrane Source: UniProtKB-KW
  • mitochondrion Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 312312Nicotinamide adenine dinucleotide transporter 1, chloroplasticPRO_0000420695Add
BLAST

Proteomic databases

PaxDbiO22261.
PRIDEiO22261.

PTM databases

iPTMnetiO22261.

Expressioni

Tissue specificityi

Highly expressed in young leaf mesophyll cells, root tips and at the branches of adventitious roots. Low expression in all flower tissues and not detected in siliques and seeds.1 Publication

Gene expression databases

GenevisibleiO22261. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G47490.1.

Structurei

3D structure databases

ProteinModelPortaliO22261.
SMRiO22261. Positions 16-309.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati11 – 10393Solcar 1Add
BLAST
Repeati111 – 19989Solcar 2Add
BLAST
Repeati211 – 29989Solcar 3Add
BLAST

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0757. Eukaryota.
ENOG410XS20. LUCA.
HOGENOMiHOG000188210.
InParanoidiO22261.
KOiK15115.
OMAiFPTWMIY.
PhylomeDBiO22261.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O22261-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSANSHPPNS KNVLCNAAAG AAAGVVAATF VCPLDVIKTR FQVHGLPKLG
60 70 80 90 100
DANIKGSLIV GSLEQIFKRE GMRGLYRGLS PTVMALLSNW AIYFTMYDQL
110 120 130 140 150
KSFLCSNDHK LSVGANVLAA SGAGAATTIA TNPLWVVKTR LQTQGMRVGI
160 170 180 190 200
VPYKSTFSAL RRIAYEEGIR GLYSGLVPAL AGISHVAIQF PTYEMIKVYL
210 220 230 240 250
AKKGDKSVDN LNARDVAVAS SIAKIFASTL TYPHEVVRAR LQEQGHHSEK
260 270 280 290 300
RYSGVRDCIK KVFEKDGFPG FYRGCATNLL RTTPAAVITF TSFEMVHRFL
310
VTHIPSEQSS IL
Length:312
Mass (Da):33,883
Last modified:June 1, 2002 - v2
Checksum:i95D5FF1039D3F634
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ871011 mRNA. Translation: CAI38582.1.
FM211595 mRNA. Translation: CAR70090.1.
AC002535 Genomic DNA. Translation: AAC62861.2.
CP002685 Genomic DNA. Translation: AEC10850.1.
AF372957 mRNA. Translation: AAK50096.1.
AY074833 mRNA. Translation: AAL69531.1.
AK316807 mRNA. Translation: BAH19521.1.
AY084889 mRNA. Translation: AAM61452.1.
PIRiT00435.
RefSeqiNP_566102.1. NM_130317.2.
UniGeneiAt.19131.

Genome annotation databases

EnsemblPlantsiAT2G47490.1; AT2G47490.1; AT2G47490.
GeneIDi819362.
GrameneiAT2G47490.1; AT2G47490.1; AT2G47490.
KEGGiath:AT2G47490.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ871011 mRNA. Translation: CAI38582.1.
FM211595 mRNA. Translation: CAR70090.1.
AC002535 Genomic DNA. Translation: AAC62861.2.
CP002685 Genomic DNA. Translation: AEC10850.1.
AF372957 mRNA. Translation: AAK50096.1.
AY074833 mRNA. Translation: AAL69531.1.
AK316807 mRNA. Translation: BAH19521.1.
AY084889 mRNA. Translation: AAM61452.1.
PIRiT00435.
RefSeqiNP_566102.1. NM_130317.2.
UniGeneiAt.19131.

3D structure databases

ProteinModelPortaliO22261.
SMRiO22261. Positions 16-309.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G47490.1.

Protein family/group databases

TCDBi2.A.29.10.11. the mitochondrial carrier (mc) family.

PTM databases

iPTMnetiO22261.

Proteomic databases

PaxDbiO22261.
PRIDEiO22261.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G47490.1; AT2G47490.1; AT2G47490.
GeneIDi819362.
GrameneiAT2G47490.1; AT2G47490.1; AT2G47490.
KEGGiath:AT2G47490.

Organism-specific databases

TAIRiAT2G47490.

Phylogenomic databases

eggNOGiKOG0757. Eukaryota.
ENOG410XS20. LUCA.
HOGENOMiHOG000188210.
InParanoidiO22261.
KOiK15115.
OMAiFPTWMIY.
PhylomeDBiO22261.

Miscellaneous databases

PROiO22261.

Gene expression databases

GenevisibleiO22261. AT.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR002067. Mit_carrier.
IPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
PRINTSiPR00926. MITOCARRIER.
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Folate metabolism in plants: an Arabidopsis homolog of the mammalian mitochondrial folate transporter mediates folate import into chloroplasts."
    Bedhomme M., Hoffmann M., McCarthy E.A., Gambonnet B., Moran R.G., Rebeille F., Ravanel S.
    J. Biol. Chem. 280:34823-34831(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Strain: cv. Wassilewskija.
  2. "Molecular identification and functional characterization of Arabidopsis thaliana mitochondrial and chloroplastic NAD+ carrier proteins."
    Palmieri F., Rieder B., Ventrella A., Blanco E., Do P.T., Nunes-Nesi A., Trauth A.U., Fiermonte G., Tjaden J., Agrimi G., Kirchberger S., Paradies E., Fernie A.R., Neuhaus H.E.
    J. Biol. Chem. 284:31249-31259(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBSTRATE SPECIFICITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  6. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  7. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

Entry informationi

Entry nameiNDT1_ARATH
AccessioniPrimary (citable) accession number: O22261
Secondary accession number(s): Q94JT4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: June 1, 2002
Last modified: June 8, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Appears to be a chloroplast envelope-located membrane protein lacking an N-terminal-located transit peptide.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.