Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nicotinamide adenine dinucleotide transporter 1, chloroplastic

Gene

NDT1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mediates the NAD+ import into chloroplast. Favors the NAD+(in)/ADP or AMP(out) antiport exchange, but is also able to catalyze a low unidirectional transport (uniport) of NAD+. Transports NAD+, nicotinic acid adenine dinucleotide, nicotinamide mononucleotide, nicotinic acid mononucleotide, FAD, FMN, TTP, TDP, TMP, UTP, UDP, UMP, CTP, CDP, CMP, GTP, GDP, GMP, 3'-AMP, ATP, ADP, AND AMP, has low transport activity with cAMP, pyrophosphate, NADH and alpha-NAD+, and has no activity with NADP+, NADPH, nicotinamide, nicotinic acid, adenosine, thiamine mono- or diphosphate, inorganic phosphate, CoA, folate, NaCl, malate, malonate, citrate, fumarate, aspartate, glutamate, S-adenosylmethionine, lysine, arginine, and ornithine.2 Publications

Miscellaneous

Appears to be a chloroplast envelope-located membrane protein lacking an N-terminal-located transit peptide.

Enzyme regulationi

Inhibited by pyridoxal 5'-phosphate, bathophenanthroline, tannic acid, mersalyl, mercuric chloride, p-hydroxymercuribenzoate, p-hydroxymercuribenzoate sulfonate, bromocresol purple and N-ethylmaleimide.1 Publication

Kineticsi

  1. KM=0.24 mM for for the NAD+/NAD+ exchange1 Publication
  1. Vmax=1.41 mmol/min/g enzyme for the NAD+/NAD+ exchange1 Publication

GO - Molecular functioni

GO - Biological processi

  • mitochondrial transport Source: GO_Central
  • NAD transmembrane transport Source: GO_Central
  • NAD transport Source: TAIR

Keywordsi

Biological processAntiport, Transport

Protein family/group databases

TCDBi2.A.29.10.11 the mitochondrial carrier (mc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Nicotinamide adenine dinucleotide transporter 1, chloroplastic
Short name:
AtNDT1
Alternative name(s):
NAD(+) transporter 1
Gene namesi
Name:NDT1
Synonyms:NADT1
Ordered Locus Names:At2g47490
ORF Names:T30B22.21
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G47490
TAIRilocus:2062002 AT2G47490

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei17 – 37Helical; Name=1Sequence analysisAdd BLAST21
Transmembranei78 – 98Helical; Name=2Sequence analysisAdd BLAST21
Transmembranei117 – 137Helical; Name=3Sequence analysisAdd BLAST21
Transmembranei171 – 191Helical; Name=4Sequence analysisAdd BLAST21
Transmembranei216 – 232Helical; Name=5Sequence analysisAdd BLAST17
Transmembranei271 – 293Helical; Name=6Sequence analysisAdd BLAST23

Keywords - Cellular componenti

Chloroplast, Membrane, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004206951 – 312Nicotinamide adenine dinucleotide transporter 1, chloroplasticAdd BLAST312

Proteomic databases

PaxDbiO22261

PTM databases

iPTMnetiO22261

Expressioni

Tissue specificityi

Highly expressed in young leaf mesophyll cells, root tips and at the branches of adventitious roots. Low expression in all flower tissues and not detected in siliques and seeds.1 Publication

Gene expression databases

ExpressionAtlasiO22261 baseline and differential
GenevisibleiO22261 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G47490.1

Structurei

3D structure databases

ProteinModelPortaliO22261
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati11 – 103Solcar 1Add BLAST93
Repeati111 – 199Solcar 2Add BLAST89
Repeati211 – 299Solcar 3Add BLAST89

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0757 Eukaryota
ENOG410XS20 LUCA
HOGENOMiHOG000188210
InParanoidiO22261
KOiK15115
OMAiLRPKYKG
OrthoDBiEOG09360FC7
PhylomeDBiO22261

Family and domain databases

Gene3Di1.50.40.10, 1 hit
InterProiView protein in InterPro
IPR002067 Mit_carrier
IPR018108 Mitochondrial_sb/sol_carrier
IPR023395 Mt_carrier_dom_sf
PfamiView protein in Pfam
PF00153 Mito_carr, 3 hits
PRINTSiPR00926 MITOCARRIER
SUPFAMiSSF103506 SSF103506, 1 hit
PROSITEiView protein in PROSITE
PS50920 SOLCAR, 3 hits

Sequencei

Sequence statusi: Complete.

O22261-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSANSHPPNS KNVLCNAAAG AAAGVVAATF VCPLDVIKTR FQVHGLPKLG
60 70 80 90 100
DANIKGSLIV GSLEQIFKRE GMRGLYRGLS PTVMALLSNW AIYFTMYDQL
110 120 130 140 150
KSFLCSNDHK LSVGANVLAA SGAGAATTIA TNPLWVVKTR LQTQGMRVGI
160 170 180 190 200
VPYKSTFSAL RRIAYEEGIR GLYSGLVPAL AGISHVAIQF PTYEMIKVYL
210 220 230 240 250
AKKGDKSVDN LNARDVAVAS SIAKIFASTL TYPHEVVRAR LQEQGHHSEK
260 270 280 290 300
RYSGVRDCIK KVFEKDGFPG FYRGCATNLL RTTPAAVITF TSFEMVHRFL
310
VTHIPSEQSS IL
Length:312
Mass (Da):33,883
Last modified:June 1, 2002 - v2
Checksum:i95D5FF1039D3F634
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ871011 mRNA Translation: CAI38582.1
FM211595 mRNA Translation: CAR70090.1
AC002535 Genomic DNA Translation: AAC62861.2
CP002685 Genomic DNA Translation: AEC10850.1
AF372957 mRNA Translation: AAK50096.1
AY074833 mRNA Translation: AAL69531.1
AK316807 mRNA Translation: BAH19521.1
AY084889 mRNA Translation: AAM61452.1
PIRiT00435
RefSeqiNP_566102.1, NM_130317.3
UniGeneiAt.19131

Genome annotation databases

EnsemblPlantsiAT2G47490.1; AT2G47490.1; AT2G47490
GeneIDi819362
GrameneiAT2G47490.1; AT2G47490.1; AT2G47490
KEGGiath:AT2G47490

Similar proteinsi

Entry informationi

Entry nameiNDT1_ARATH
AccessioniPrimary (citable) accession number: O22261
Secondary accession number(s): Q94JT4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: June 1, 2002
Last modified: April 25, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health