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O22256 (PME20_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable pectinesterase/pectinesterase inhibitor 20

Including the following 2 domains:

  1. Pectinesterase inhibitor 20
    Alternative name(s):
    Pectin methylesterase inhibitor 20
  2. Pectinesterase 20
    Short name=PE 20
    EC=3.1.1.11
    Alternative name(s):
    Pectin methylesterase 20
    Short name=AtPME20
Gene names
Name:PME20
Synonyms:ARATH20
Ordered Locus Names:At2g47550
ORF Names:T30B22.15
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length560 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Acts in the modification of cell walls via demethylesterification of cell wall pectin By similarity.

Catalytic activity

Pectin + n H2O = n methanol + pectate.

Pathway

Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.

Subcellular location

Secreted › cell wall By similarity.

Tissue specificity

Expressed in flower buds. Ref.5

Miscellaneous

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.

Sequence similarities

In the N-terminal section; belongs to the PMEI family.

In the C-terminal section; belongs to the pectinesterase family.

Sequence caution

The sequence AAL24207.1 differs from that shown. Reason: Frameshift at position 183.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 560536Probable pectinesterase/pectinesterase inhibitor 20
PRO_0000371676

Regions

Region25 – 179155Pectinesterase inhibitor 20
Region246 – 544299Pectinesterase 20

Sites

Active site3761Proton donor; for pectinesterase activity By similarity
Active site3971Nucleophile; for pectinesterase activity By similarity
Binding site3231Substrate; for pectinesterase activity By similarity
Binding site3531Substrate; for pectinesterase activity By similarity
Binding site4651Substrate; for pectinesterase activity By similarity
Binding site4671Substrate; for pectinesterase activity By similarity
Site3751Transition state stabilizer By similarity

Amino acid modifications

Glycosylation311N-linked (GlcNAc...) Potential
Glycosylation1681N-linked (GlcNAc...) Potential
Glycosylation2511N-linked (GlcNAc...) Potential
Glycosylation2551N-linked (GlcNAc...) Potential
Glycosylation2681N-linked (GlcNAc...) Potential
Glycosylation3071N-linked (GlcNAc...) Potential
Glycosylation3141N-linked (GlcNAc...) Potential
Glycosylation3401N-linked (GlcNAc...) Potential
Glycosylation4561N-linked (GlcNAc...) Potential
Glycosylation5071N-linked (GlcNAc...) Potential
Glycosylation5281N-linked (GlcNAc...) Potential
Glycosylation5341N-linked (GlcNAc...) Potential
Disulfide bond390 ↔ 410 By similarity

Sequences

Sequence LengthMass (Da)Tools
O22256 [UniParc].

Last modified June 1, 2002. Version 2.
Checksum: 4E6EDAE083A9A27C

FASTA56061,497
        10         20         30         40         50         60 
MSQKLMFLFT LACLSSLPSP FISAQIPAIG NATSPSNICR FAPDPSYCRS VLPNQPGDIY 

        70         80         90        100        110        120 
SYGRLSLRRS LSRARRFISM IDAELDRKGK VAAKSTVGAL EDCKFLASLT MDYLLSSSQT 

       130        140        150        160        170        180 
ADSTKTLSLS RAEDVHTFLS AAITNEQTCL EGLKSTASEN GLSGDLFNDT KLYGVSLALF 

       190        200        210        220        230        240 
SKGWVPRRQR SRPIWQPQAR FKKFFGFRNG KLPLKMTERA RAVYNTVTRR KLLQSDADAV 

       250        260        270        280        290        300 
QVSDIVTVIQ NGTGNFTTIN AAIAAAPNKT DGSNGYFLIY VTAGLYEEYV EVPKNKRYVM 

       310        320        330        340        350        360 
MIGDGINQTV ITGNRSVVDG WTTFNSATFI LSGPNFIGVN ITIRNTAGPT KGQAVALRSG 

       370        380        390        400        410        420 
GDLSVFYSCS FEAYQDTLYT HSLRQFYREC DVYGTVDFIF GNAAVVLQNC NLYPRQPRKG 

       430        440        450        460        470        480 
QSNEVTAQGR TDPNQNTGTA IHGCTIRPAD DLATSNYTVK TYLGRPWKEY SRTVVMQTYI 

       490        500        510        520        530        540 
DGFLEPSGWN AWSGDFALST LYYAEYNNTG PGSDTTNRVT WPGYHVINAT DASNFTVTNF 

       550        560 
LVGEGWIGQT GVPFVGGLIA

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed: 10617197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed: 14593172] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Pectin methylesterases: sequence-structural features and phylogenetic relationships."
Markovic O., Janecek S.
Carbohydr. Res. 339:2281-2295(2004) [PubMed: 15337457] [Abstract]
Cited for: GENE FAMILY, NOMENCLATURE.
[5]"Comprehensive expression profiling of the pectin methylesterase gene family during silique development in Arabidopsis thaliana."
Louvet R., Cavel E., Gutierrez L., Guenin S., Roger D., Gillet F., Guerineau F., Pelloux J.
Planta 224:782-791(2006) [PubMed: 16622707] [Abstract]
Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AC002535 Genomic DNA. Translation: AAC62855.2.
CP002685 Genomic DNA. Translation: AEC10857.1.
AY058099 mRNA. Translation: AAL24207.1. Frameshift.
BT002700 mRNA. Translation: AAO11616.1.
IPIIPI00529375.
PIRT00429.
RefSeqNP_566103.1. NM_130323.3.
UniGeneAt.12430.
At.66383.

3D structure databases

HSSPHSSP built from PDB template 1XG2 based on UniProtKB P14280.
ProteinModelPortalO22256.
SMRO22256. Positions 242-559.
ModBaseSearch...

Proteomic databases

PRIDEO22256.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G47550.1; AT2G47550.1; AT2G47550.
GeneID819368.
GenomeReviewsGene locus AT2G47550 in contig CT485783_GR.
KEGGath:AT2G47550.
NMPDRfig|3702.1.peg.11943.

Organism-specific databases

GeneFarm232. 8.
TAIRAt2g47550.

Phylogenomic databases

eggNOGeuNOG17330.
GeneTreeEPGT00070000027901.
HOGENOMHBG747179.
InParanoidO22256.
OMAIAAAPNK.
PhylomeDBO22256.
ProtClustDBPLN02713.

Gene expression databases

ArrayExpressO22256.
GenevestigatorO22256.

Family and domain databases

InterProIPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR018040. Pectinesterase_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib.
[Graphical view]
Gene3DG3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
G3DSA:1.20.140.40. Pectinesterase_inhib. 1 hit.
PfamPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
SMARTSM00856. PMEI. 1 hit.
[Graphical view]
SUPFAMSSF51126. Pectin_lyas_like. 1 hit.
SSF101148. Pectinesterase_inhib. 1 hit.
PROSITEPS00800. PECTINESTERASE_1. False negative.
PS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePME20_ARATH
AccessionPrimary (citable) accession number: O22256
Secondary accession number(s): Q8GUF8, Q93Z62
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: June 1, 2002
Last modified: December 14, 2011
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents