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O22241

- AROD4_ARATH

UniProt

O22241 - AROD4_ARATH

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Protein

Arogenate dehydratase 4, chloroplastic

Gene

ADT4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Converts the prephenate produced from the shikimate-chorismate pathway into phenylalanine.1 Publication

Catalytic activityi

L-arogenate = L-phenylalanine + H2O + CO2.

Kineticsi

  1. KM=10.08 mM for arogenate1 Publication

Vmax=52.32 pmol/sec/µg enzyme with arogenate as substrate1 Publication

Pathwayi

GO - Molecular functioni

  1. amino acid binding Source: InterPro
  2. arogenate dehydratase activity Source: TAIR
  3. prephenate dehydratase activity Source: InterPro

GO - Biological processi

  1. L-phenylalanine biosynthetic process Source: UniProtKB-UniPathway
  2. response to karrikin Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Phenylalanine biosynthesis

Enzyme and pathway databases

BioCyciARA:AT3G44720-MONOMER.
BRENDAi4.2.1.91. 399.
SABIO-RKO22241.
UniPathwayiUPA00121; UER00344.

Names & Taxonomyi

Protein namesi
Recommended name:
Arogenate dehydratase 4, chloroplastic (EC:4.2.1.91)
Short name:
AtADT4
Gene namesi
Name:ADT4
Ordered Locus Names:At3g44720
ORF Names:T32N15.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 3

Organism-specific databases

TAIRiAT3G44720.

Subcellular locationi

Plastidchloroplast stroma 1 Publication

GO - Cellular componenti

  1. chloroplast Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3434ChloroplastSequence AnalysisAdd
BLAST
Chaini35 – 424390Arogenate dehydratase 4, chloroplasticPRO_0000373793Add
BLAST

Proteomic databases

PaxDbiO22241.
PRIDEiO22241.

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems, flowers and siliques. More abundant in stems and roots.1 Publication

Gene expression databases

GenevestigatoriO22241.

Interactioni

Protein-protein interaction databases

BioGridi8921. 10 interactions.
IntActiO22241. 13 interactions.
STRINGi3702.AT3G44720.1-P.

Structurei

3D structure databases

ProteinModelPortaliO22241.
SMRiO22241. Positions 127-409.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini126 – 303178Prephenate dehydratasePROSITE-ProRule annotationAdd
BLAST
Domaini319 – 41092ACTPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi244 – 27633Ala-richAdd
BLAST

Sequence similaritiesi

Contains 1 ACT domain.PROSITE-ProRule annotation
Contains 1 prephenate dehydratase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0077.
HOGENOMiHOG000018970.
InParanoidiO22241.
KOiK05359.
OMAiSLAMATH.
PhylomeDBiO22241.

Family and domain databases

InterProiIPR002912. ACT_dom.
IPR001086. Preph_deHydtase.
IPR018528. Preph_deHydtase_CS.
[Graphical view]
PfamiPF00800. PDT. 1 hit.
[Graphical view]
PROSITEiPS51671. ACT. 1 hit.
PS00857. PREPHENATE_DEHYDR_1. 1 hit.
PS00858. PREPHENATE_DEHYDR_2. 1 hit.
PS51171. PREPHENATE_DEHYDR_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O22241-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQAATSCDLK FRSTDPTSRN KCFSHAIPKR VAVTCGYRSE SFSFPNGVSV
60 70 80 90 100
SRSDWQSSCA ILSSKVASVE NTGGLADKIA AVNGHTNGSV NLGLVAVEST
110 120 130 140 150
NGKLAPAQPL TITDLSPAPL HGSSLRVAYQ GVPGAYSEAA AGKAYPNCDA
160 170 180 190 200
IPCDQFDVAF QAVELWIADR AVLPVENSLG GSIHRNYDLL LRHRLHIVGE
210 220 230 240 250
VQIPVHHCLL ALPGVRTDCV SRVISHPQAL AQTEHSLDVL TPHAAREAFH
260 270 280 290 300
DTAAAAEYIS ANDLHDTAAV ASARAAELYN LQILADGIQD DPGNVTRFLM
310 320 330 340 350
LAREPIIPRT DRPFKTSIVF AAQEHKGTSV LFKVLSAFAF RDISLTKIES
360 370 380 390 400
RPHHNRPLRV VGDGSFGTSK NFEYMFYVDF EASMAEPRAQ NALAEVQEYT
410 420
SFLRVLGSYP MDMTPWSMTS TEEA
Length:424
Mass (Da):45,925
Last modified:January 1, 1998 - v1
Checksum:iB12A05BE6C0A0D24
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ411467 mRNA. Translation: ABD67753.1.
AC002534 Genomic DNA. Translation: AAB70035.1.
CP002686 Genomic DNA. Translation: AEE77939.1.
AY062692 mRNA. Translation: AAL32770.1.
BT008862 mRNA. Translation: AAP68301.1.
RefSeqiNP_190058.1. NM_114340.3.
UniGeneiAt.22683.
At.5118.
At.67075.

Genome annotation databases

EnsemblPlantsiAT3G44720.1; AT3G44720.1; AT3G44720.
GeneIDi823601.
KEGGiath:AT3G44720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ411467 mRNA. Translation: ABD67753.1 .
AC002534 Genomic DNA. Translation: AAB70035.1 .
CP002686 Genomic DNA. Translation: AEE77939.1 .
AY062692 mRNA. Translation: AAL32770.1 .
BT008862 mRNA. Translation: AAP68301.1 .
RefSeqi NP_190058.1. NM_114340.3.
UniGenei At.22683.
At.5118.
At.67075.

3D structure databases

ProteinModelPortali O22241.
SMRi O22241. Positions 127-409.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 8921. 10 interactions.
IntActi O22241. 13 interactions.
STRINGi 3702.AT3G44720.1-P.

Proteomic databases

PaxDbi O22241.
PRIDEi O22241.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT3G44720.1 ; AT3G44720.1 ; AT3G44720 .
GeneIDi 823601.
KEGGi ath:AT3G44720.

Organism-specific databases

TAIRi AT3G44720.

Phylogenomic databases

eggNOGi COG0077.
HOGENOMi HOG000018970.
InParanoidi O22241.
KOi K05359.
OMAi SLAMATH.
PhylomeDBi O22241.

Enzyme and pathway databases

UniPathwayi UPA00121 ; UER00344 .
BioCyci ARA:AT3G44720-MONOMER.
BRENDAi 4.2.1.91. 399.
SABIO-RK O22241.

Gene expression databases

Genevestigatori O22241.

Family and domain databases

InterProi IPR002912. ACT_dom.
IPR001086. Preph_deHydtase.
IPR018528. Preph_deHydtase_CS.
[Graphical view ]
Pfami PF00800. PDT. 1 hit.
[Graphical view ]
PROSITEi PS51671. ACT. 1 hit.
PS00857. PREPHENATE_DEHYDR_1. 1 hit.
PS00858. PREPHENATE_DEHYDR_2. 1 hit.
PS51171. PREPHENATE_DEHYDR_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Matringe M., Grisollet D., Rippert P.
    Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Phenylalanine biosynthesis in Arabidopsis thaliana. Identification and characterization of arogenate dehydratases."
    Cho M.-H., Corea O.R.A., Yang H., Bedgar D.L., Laskar D.D., Anterola A.M., Moog-Anterola F.A., Hood R.L., Kohalmi S.E., Bernards M.A., Kang C., Davin L.B., Lewis N.G.
    J. Biol. Chem. 282:30827-30835(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES.
  6. "Tyrosine and phenylalanine are synthesized within the plastids in Arabidopsis."
    Rippert P., Puyaubert J., Grisollet D., Derrier L., Matringe M.
    Plant Physiol. 149:1251-1260(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiAROD4_ARATH
AccessioniPrimary (citable) accession number: O22241
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Has no detectable prehenate dehydratase activity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3