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Protein

Arogenate dehydratase 4, chloroplastic

Gene

ADT4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts the prephenate produced from the shikimate-chorismate pathway into phenylalanine.1 Publication

Miscellaneous

Has no detectable prehenate dehydratase activity.

Catalytic activityi

L-arogenate = L-phenylalanine + H2O + CO2.

Kineticsi

  1. KM=10.08 mM for arogenate1 Publication
  1. Vmax=52.32 pmol/sec/µg enzyme with arogenate as substrate1 Publication

Pathwayi: L-phenylalanine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-phenylalanine from L-arogenate.
Proteins known to be involved in this subpathway in this organism are:
  1. Arogenate dehydratase/prephenate dehydratase 1, chloroplastic (ADT1), Arogenate dehydratase (AXX17_At2g23810), Arogenate dehydratase (AXX17_At1g08050), Arogenate dehydratase 5, chloroplastic (ADT5), Arogenate dehydratase 4, chloroplastic (ADT4), Arogenate dehydratase/prephenate dehydratase 2, chloroplastic (ADT2), Arogenate dehydratase 3, chloroplastic (ADT3), Arogenate dehydratase (AXX17_At5g22060), Arogenate dehydratase (AXX17_At3g07630), Arogenate dehydratase/prephenate dehydratase 6, chloroplastic (ADT6), Arogenate dehydratase (AXX17_At3g38410), Arogenate dehydratase (AXX17_At1g12160)
This subpathway is part of the pathway L-phenylalanine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-phenylalanine from L-arogenate, the pathway L-phenylalanine biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

  • arogenate dehydratase activity Source: TAIR

GO - Biological processi

  • L-phenylalanine biosynthetic process Source: GO_Central
  • response to karrikin Source: TAIR

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Phenylalanine biosynthesis

Enzyme and pathway databases

BioCyciARA:AT3G44720-MONOMER
BRENDAi4.2.1.91 399
SABIO-RKiO22241
UniPathwayiUPA00121; UER00344

Names & Taxonomyi

Protein namesi
Recommended name:
Arogenate dehydratase 4, chloroplastic (EC:4.2.1.91)
Short name:
AtADT4
Gene namesi
Name:ADT4
Ordered Locus Names:At3g44720
ORF Names:T32N15.11
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi

Organism-specific databases

AraportiAT3G44720
TAIRilocus:2101630 AT3G44720

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 34ChloroplastSequence analysisAdd BLAST34
ChainiPRO_000037379335 – 424Arogenate dehydratase 4, chloroplasticAdd BLAST390

Proteomic databases

PaxDbiO22241
PRIDEiO22241

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems, flowers and siliques. More abundant in stems and roots.1 Publication

Gene expression databases

ExpressionAtlasiO22241 baseline and differential
GenevisibleiO22241 AT

Interactioni

Protein-protein interaction databases

BioGridi8921, 10 interactors
IntActiO22241, 13 interactors
STRINGi3702.AT3G44720.1

Structurei

3D structure databases

ProteinModelPortaliO22241
SMRiO22241
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini126 – 303Prephenate dehydratasePROSITE-ProRule annotationAdd BLAST178
Domaini319 – 410ACTPROSITE-ProRule annotationAdd BLAST92

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi244 – 276Ala-richAdd BLAST33

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2797 Eukaryota
COG0077 LUCA
HOGENOMiHOG000018970
InParanoidiO22241
KOiK05359
OMAiSHAIPKR
OrthoDBiEOG093609W8
PhylomeDBiO22241

Family and domain databases

InterProiView protein in InterPro
IPR002912 ACT_dom
IPR001086 Preph_deHydtase
IPR018528 Preph_deHydtase_CS
PfamiView protein in Pfam
PF00800 PDT, 1 hit
PROSITEiView protein in PROSITE
PS51671 ACT, 1 hit
PS00857 PREPHENATE_DEHYDR_1, 1 hit
PS00858 PREPHENATE_DEHYDR_2, 1 hit
PS51171 PREPHENATE_DEHYDR_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O22241-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQAATSCDLK FRSTDPTSRN KCFSHAIPKR VAVTCGYRSE SFSFPNGVSV
60 70 80 90 100
SRSDWQSSCA ILSSKVASVE NTGGLADKIA AVNGHTNGSV NLGLVAVEST
110 120 130 140 150
NGKLAPAQPL TITDLSPAPL HGSSLRVAYQ GVPGAYSEAA AGKAYPNCDA
160 170 180 190 200
IPCDQFDVAF QAVELWIADR AVLPVENSLG GSIHRNYDLL LRHRLHIVGE
210 220 230 240 250
VQIPVHHCLL ALPGVRTDCV SRVISHPQAL AQTEHSLDVL TPHAAREAFH
260 270 280 290 300
DTAAAAEYIS ANDLHDTAAV ASARAAELYN LQILADGIQD DPGNVTRFLM
310 320 330 340 350
LAREPIIPRT DRPFKTSIVF AAQEHKGTSV LFKVLSAFAF RDISLTKIES
360 370 380 390 400
RPHHNRPLRV VGDGSFGTSK NFEYMFYVDF EASMAEPRAQ NALAEVQEYT
410 420
SFLRVLGSYP MDMTPWSMTS TEEA
Length:424
Mass (Da):45,925
Last modified:January 1, 1998 - v1
Checksum:iB12A05BE6C0A0D24
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ411467 mRNA Translation: ABD67753.1
AC002534 Genomic DNA Translation: AAB70035.1
CP002686 Genomic DNA Translation: AEE77939.1
AY062692 mRNA Translation: AAL32770.1
BT008862 mRNA Translation: AAP68301.1
RefSeqiNP_190058.1, NM_114340.4
UniGeneiAt.22683
At.5118
At.67075

Genome annotation databases

EnsemblPlantsiAT3G44720.1; AT3G44720.1; AT3G44720
GeneIDi823601
GrameneiAT3G44720.1; AT3G44720.1; AT3G44720
KEGGiath:AT3G44720

Entry informationi

Entry nameiAROD4_ARATH
AccessioniPrimary (citable) accession number: O22241
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

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