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O22241

- AROD4_ARATH

UniProt

O22241 - AROD4_ARATH

Protein

Arogenate dehydratase 4, chloroplastic

Gene

ADT4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Converts the prephenate produced from the shikimate-chorismate pathway into phenylalanine.1 Publication

    Catalytic activityi

    L-arogenate = L-phenylalanine + H2O + CO2.

    Kineticsi

    1. KM=10.08 mM for arogenate1 Publication

    Vmax=52.32 pmol/sec/µg enzyme with arogenate as substrate1 Publication

    Pathwayi

    GO - Molecular functioni

    1. amino acid binding Source: InterPro
    2. arogenate dehydratase activity Source: TAIR
    3. prephenate dehydratase activity Source: InterPro

    GO - Biological processi

    1. L-phenylalanine biosynthetic process Source: UniProtKB-UniPathway
    2. response to karrikin Source: TAIR

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Phenylalanine biosynthesis

    Enzyme and pathway databases

    BioCyciARA:AT3G44720-MONOMER.
    BRENDAi4.2.1.91. 399.
    SABIO-RKO22241.
    UniPathwayiUPA00121; UER00344.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Arogenate dehydratase 4, chloroplastic (EC:4.2.1.91)
    Short name:
    AtADT4
    Gene namesi
    Name:ADT4
    Ordered Locus Names:At3g44720
    ORF Names:T32N15.11
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 3

    Organism-specific databases

    TAIRiAT3G44720.

    Subcellular locationi

    Plastidchloroplast stroma 1 Publication

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast stroma Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3434ChloroplastSequence AnalysisAdd
    BLAST
    Chaini35 – 424390Arogenate dehydratase 4, chloroplasticPRO_0000373793Add
    BLAST

    Proteomic databases

    PaxDbiO22241.
    PRIDEiO22241.

    Expressioni

    Tissue specificityi

    Expressed in roots, leaves, stems, flowers and siliques. More abundant in stems and roots.1 Publication

    Gene expression databases

    GenevestigatoriO22241.

    Interactioni

    Protein-protein interaction databases

    BioGridi8921. 10 interactions.
    IntActiO22241. 13 interactions.
    STRINGi3702.AT3G44720.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO22241.
    SMRiO22241. Positions 127-409.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini126 – 303178Prephenate dehydratasePROSITE-ProRule annotationAdd
    BLAST
    Domaini319 – 41092ACTPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi244 – 27633Ala-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 ACT domain.PROSITE-ProRule annotation
    Contains 1 prephenate dehydratase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0077.
    HOGENOMiHOG000018970.
    InParanoidiO22241.
    KOiK05359.
    OMAiSLAMATH.
    PhylomeDBiO22241.

    Family and domain databases

    InterProiIPR002912. ACT_dom.
    IPR001086. Preph_deHydtase.
    IPR018528. Preph_deHydtase_CS.
    [Graphical view]
    PfamiPF00800. PDT. 1 hit.
    [Graphical view]
    PROSITEiPS51671. ACT. 1 hit.
    PS00857. PREPHENATE_DEHYDR_1. 1 hit.
    PS00858. PREPHENATE_DEHYDR_2. 1 hit.
    PS51171. PREPHENATE_DEHYDR_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O22241-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQAATSCDLK FRSTDPTSRN KCFSHAIPKR VAVTCGYRSE SFSFPNGVSV    50
    SRSDWQSSCA ILSSKVASVE NTGGLADKIA AVNGHTNGSV NLGLVAVEST 100
    NGKLAPAQPL TITDLSPAPL HGSSLRVAYQ GVPGAYSEAA AGKAYPNCDA 150
    IPCDQFDVAF QAVELWIADR AVLPVENSLG GSIHRNYDLL LRHRLHIVGE 200
    VQIPVHHCLL ALPGVRTDCV SRVISHPQAL AQTEHSLDVL TPHAAREAFH 250
    DTAAAAEYIS ANDLHDTAAV ASARAAELYN LQILADGIQD DPGNVTRFLM 300
    LAREPIIPRT DRPFKTSIVF AAQEHKGTSV LFKVLSAFAF RDISLTKIES 350
    RPHHNRPLRV VGDGSFGTSK NFEYMFYVDF EASMAEPRAQ NALAEVQEYT 400
    SFLRVLGSYP MDMTPWSMTS TEEA 424
    Length:424
    Mass (Da):45,925
    Last modified:January 1, 1998 - v1
    Checksum:iB12A05BE6C0A0D24
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ411467 mRNA. Translation: ABD67753.1.
    AC002534 Genomic DNA. Translation: AAB70035.1.
    CP002686 Genomic DNA. Translation: AEE77939.1.
    AY062692 mRNA. Translation: AAL32770.1.
    BT008862 mRNA. Translation: AAP68301.1.
    RefSeqiNP_190058.1. NM_114340.3.
    UniGeneiAt.22683.
    At.5118.
    At.67075.

    Genome annotation databases

    EnsemblPlantsiAT3G44720.1; AT3G44720.1; AT3G44720.
    GeneIDi823601.
    KEGGiath:AT3G44720.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ411467 mRNA. Translation: ABD67753.1 .
    AC002534 Genomic DNA. Translation: AAB70035.1 .
    CP002686 Genomic DNA. Translation: AEE77939.1 .
    AY062692 mRNA. Translation: AAL32770.1 .
    BT008862 mRNA. Translation: AAP68301.1 .
    RefSeqi NP_190058.1. NM_114340.3.
    UniGenei At.22683.
    At.5118.
    At.67075.

    3D structure databases

    ProteinModelPortali O22241.
    SMRi O22241. Positions 127-409.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 8921. 10 interactions.
    IntActi O22241. 13 interactions.
    STRINGi 3702.AT3G44720.1-P.

    Proteomic databases

    PaxDbi O22241.
    PRIDEi O22241.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT3G44720.1 ; AT3G44720.1 ; AT3G44720 .
    GeneIDi 823601.
    KEGGi ath:AT3G44720.

    Organism-specific databases

    TAIRi AT3G44720.

    Phylogenomic databases

    eggNOGi COG0077.
    HOGENOMi HOG000018970.
    InParanoidi O22241.
    KOi K05359.
    OMAi SLAMATH.
    PhylomeDBi O22241.

    Enzyme and pathway databases

    UniPathwayi UPA00121 ; UER00344 .
    BioCyci ARA:AT3G44720-MONOMER.
    BRENDAi 4.2.1.91. 399.
    SABIO-RK O22241.

    Gene expression databases

    Genevestigatori O22241.

    Family and domain databases

    InterProi IPR002912. ACT_dom.
    IPR001086. Preph_deHydtase.
    IPR018528. Preph_deHydtase_CS.
    [Graphical view ]
    Pfami PF00800. PDT. 1 hit.
    [Graphical view ]
    PROSITEi PS51671. ACT. 1 hit.
    PS00857. PREPHENATE_DEHYDR_1. 1 hit.
    PS00858. PREPHENATE_DEHYDR_2. 1 hit.
    PS51171. PREPHENATE_DEHYDR_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Matringe M., Grisollet D., Rippert P.
      Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
      Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
      , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
      Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Phenylalanine biosynthesis in Arabidopsis thaliana. Identification and characterization of arogenate dehydratases."
      Cho M.-H., Corea O.R.A., Yang H., Bedgar D.L., Laskar D.D., Anterola A.M., Moog-Anterola F.A., Hood R.L., Kohalmi S.E., Bernards M.A., Kang C., Davin L.B., Lewis N.G.
      J. Biol. Chem. 282:30827-30835(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES.
    6. "Tyrosine and phenylalanine are synthesized within the plastids in Arabidopsis."
      Rippert P., Puyaubert J., Grisollet D., Derrier L., Matringe M.
      Plant Physiol. 149:1251-1260(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiAROD4_ARATH
    AccessioniPrimary (citable) accession number: O22241
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Has no detectable prehenate dehydratase activity.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3