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Protein

NADPH-dependent thioredoxin reductase 3

Gene

NTRC

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Thioredoxin reductase (TR) that exhibits both TR and thioredoxin (Trx) activities. Contains a C-terminal functional Trx domain. Functions as an electron donor for plastidial 2-Cys peroxiredoxins and participates in a NADPH-dependent hydrogen peroxide scavenging system in chloroplasts in the dark. Required for chlorophyll biosynthesis and biogenesis of the photosynthetic apparatus. Activates aerobic cyclase which converts Mg-protoporhyrin monomethyl ester into protochlorophyllide. Involved in a light-dependent regulation of starch biosynthesis by redox activation of the ADP-glucose pyrophosphorylase (AGPase), a central enzyme of starch synthesis.3 Publications

Catalytic activityi

Thioredoxin + NADP+ = thioredoxin disulfide + NADPH.

Cofactori

FADBy similarityNote: Binds 1 FAD per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei133FADBy similarity1
Binding sitei166FAD; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei220FADBy similarity1
Sitei241Important for activityBy similarity1
Sitei259Important for activityBy similarity1
Sitei260Important for activityBy similarity1
Binding sitei364FADBy similarity1
Active sitei454NucleophileBy similarity1
Active sitei457NucleophileBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi91 – 94FADBy similarity4
Nucleotide bindingi112 – 113FADBy similarity2
Nucleotide bindingi120 – 124FADBy similarity5
Nucleotide bindingi371 – 373FADBy similarity3

GO - Molecular functioni

  • enzyme activator activity Source: UniProtKB
  • identical protein binding Source: EnsemblPlants
  • oxidoreductase activity, acting on a sulfur group of donors, disulfide as acceptor Source: UniProtKB
  • thioredoxin-disulfide reductase activity Source: UniProtKB

GO - Biological processi

  • cell redox homeostasis Source: UniProtKB
  • hydrogen peroxide catabolic process Source: TAIR
  • positive regulation of catalytic activity Source: UniProtKB
  • regulation of chlorophyll biosynthetic process Source: UniProtKB
  • regulation of starch biosynthetic process Source: UniProtKB
  • removal of superoxide radicals Source: InterPro

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Transport
LigandFAD, Flavoprotein, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH-dependent thioredoxin reductase 3 (EC:1.8.1.9)
Short name:
NTR3
Alternative name(s):
NADPH-dependent thioredoxin reductase C
Short name:
ANTR-C
Short name:
AtNTRC
Gene namesi
Name:NTRC
Ordered Locus Names:At2g41680
ORF Names:T32G6.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G41680
TAIRilocus:2062683 AT2G41680

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

Pathology & Biotechi

Disruption phenotypei

Dwarf plants with chlorotic leaves. Accumulation of Mg-protoporhyrin after feeding with 5-aminolevulinic acid.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 67ChloroplastSequence analysisAdd BLAST67
ChainiPRO_000039455268 – 529NADPH-dependent thioredoxin reductase 3Add BLAST462

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi217 ↔ 220Redox-activePROSITE-ProRule annotation
Disulfide bondi454 ↔ 457Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiO22229

Expressioni

Gene expression databases

ExpressionAtlasiO22229 baseline and differential
GenevisibleiO22229 AT

Interactioni

Subunit structurei

May homodimerize. Interacts with the 2-Cys peroxiredoxin BAS1.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi4103, 14 interactors
IntActiO22229, 10 interactors
MINTiO22229
STRINGi3702.AT2G41680.1

Structurei

3D structure databases

ProteinModelPortaliO22229
SMRiO22229
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini403 – 529ThioredoxinPROSITE-ProRule annotationAdd BLAST127

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi72 – 78Poly-Ser7

Sequence similaritiesi

Keywords - Domaini

Redox-active center, Transit peptide

Phylogenomic databases

eggNOGiKOG0404 Eukaryota
KOG0907 Eukaryota
COG0492 LUCA
COG0526 LUCA
HOGENOMiHOG000072912
InParanoidiO22229
KOiK00384
OMAiYQEDVEF
OrthoDBiEOG09360E6S
PhylomeDBiO22229

Family and domain databases

Gene3Di3.50.50.60, 3 hits
InterProiView protein in InterPro
IPR036188 FAD/NAD-bd_sf
IPR023753 FAD/NAD-binding_dom
IPR008255 Pyr_nucl-diS_OxRdtase_2_AS
IPR000103 Pyridine_nuc-diS_OxRdtase_2
IPR005982 Thioredox_Rdtase
IPR036249 Thioredoxin-like_sf
IPR017937 Thioredoxin_CS
IPR013766 Thioredoxin_domain
PfamiView protein in Pfam
PF07992 Pyr_redox_2, 1 hit
PF00085 Thioredoxin, 1 hit
PRINTSiPR00469 PNDRDTASEII
SUPFAMiSSF51905 SSF51905, 1 hit
SSF52833 SSF52833, 1 hit
TIGRFAMsiTIGR01292 TRX_reduct, 1 hit
PROSITEiView protein in PROSITE
PS00573 PYRIDINE_REDOX_2, 1 hit
PS00194 THIOREDOXIN_1, 1 hit
PS51352 THIOREDOXIN_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O22229-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASPKIGIG IASVSSPHRV SAASSALSPP PHLFFLTTTT TTRHGGSYLL
60 70 80 90 100
RQPTRTRSSD SLRLRVSATA NSPSSSSSGG EIIENVVIIG SGPAGYTAAI
110 120 130 140 150
YAARANLKPV VFEGYQMGGV PGGQLMTTTE VENFPGFPDG ITGPDLMEKM
160 170 180 190 200
RKQAERWGAE LYPEDVESLS VTTAPFTVQT SERKVKCHSI IYATGATARR
210 220 230 240 250
LRLPREEEFW SRGISACAIC DGASPLFKGQ VLAVVGGGDT ATEEALYLTK
260 270 280 290 300
YARHVHLLVR RDQLRASKAM QDRVINNPNI TVHYNTETVD VLSNTKGQMS
310 320 330 340 350
GILLRRLDTG EETELEAKGL FYGIGHSPNS QLLEGQVELD SSGYVLVREG
360 370 380 390 400
TSNTSVEGVF AAGDVQDHEW RQAVTAAGSG CIAALSAERY LTSNNLLVEF
410 420 430 440 450
HQPQTEEAKK EFTQRDVQEK FDITLTKHKG QYALRKLYHE SPRVILVLYT
460 470 480 490 500
SPTCGPCRTL KPILNKVVDE YNHDVHFVEI DIEEDQEIAE AAGIMGTPCV
510 520
QFFKNKEMLR TISGVKMKKE YREFIEANK
Length:529
Mass (Da):57,950
Last modified:June 1, 2002 - v2
Checksum:i53853134652D64AA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti399E → K in AAN18085 (PubMed:14593172).Curated1
Sequence conflicti399E → K in AAL08250 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002510 Genomic DNA Translation: AAB84351.2
CP002685 Genomic DNA Translation: AEC10015.1
AY056394 mRNA Translation: AAL08250.1
BT000516 mRNA Translation: AAN18085.1
AK229969 mRNA Translation: BAF01794.1
PIRiT00824
RefSeqiNP_565954.1, NM_129731.6
UniGeneiAt.12409
At.42799

Genome annotation databases

EnsemblPlantsiAT2G41680.1; AT2G41680.1; AT2G41680
GeneIDi818766
GrameneiAT2G41680.1; AT2G41680.1; AT2G41680
KEGGiath:AT2G41680

Similar proteinsi

Entry informationi

Entry nameiTRXB3_ARATH
AccessioniPrimary (citable) accession number: O22229
Secondary accession number(s): Q0WM62, Q93ZQ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 15, 2010
Last sequence update: June 1, 2002
Last modified: April 25, 2018
This is version 126 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health