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Protein

Calcium-transporting ATPase 4, plasma membrane-type

Gene

ACA4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into small vacuoles.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Enzyme regulationi

Activated by calmodulin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei4514-aspartylphosphate intermediateBy similarity1
Metal bindingi749MagnesiumBy similarity1
Metal bindingi753MagnesiumBy similarity1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • calcium-transporting ATPase activity Source: TAIR
  • calmodulin binding Source: TAIR
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • anion homeostasis Source: TAIR
  • defense response to bacterium Source: TAIR
  • negative regulation of programmed cell death Source: TAIR
  • response to nematode Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G41560-MONOMER.
ReactomeiR-ATH-418359. Reduction of cytosolic Ca++ levels.
R-ATH-5578775. Ion homeostasis.
R-ATH-936837. Ion transport by P-type ATPases.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase 4, plasma membrane-type (EC:3.6.3.8)
Alternative name(s):
Ca(2+)-ATPase isoform 4
Gene namesi
Name:ACA4
Ordered Locus Names:At2g41560
ORF Names:T32G6.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G41560.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 157CytoplasmicSequence analysisAdd BLAST157
Transmembranei158 – 178HelicalSequence analysisAdd BLAST21
Topological domaini179 – 196LumenalSequence analysisAdd BLAST18
Transmembranei197 – 217HelicalSequence analysisAdd BLAST21
Topological domaini218 – 345CytoplasmicSequence analysisAdd BLAST128
Transmembranei346 – 365HelicalSequence analysisAdd BLAST20
Topological domaini366 – 395LumenalSequence analysisAdd BLAST30
Transmembranei396 – 413HelicalSequence analysisAdd BLAST18
Topological domaini414 – 804CytoplasmicSequence analysisAdd BLAST391
Transmembranei805 – 823HelicalSequence analysisAdd BLAST19
Topological domaini824 – 834LumenalSequence analysisAdd BLAST11
Transmembranei835 – 855HelicalSequence analysisAdd BLAST21
Topological domaini856 – 875CytoplasmicSequence analysisAdd BLAST20
Transmembranei876 – 898HelicalSequence analysisAdd BLAST23
Topological domaini899 – 910LumenalSequence analysisAdd BLAST12
Transmembranei911 – 932HelicalSequence analysisAdd BLAST22
Topological domaini933 – 950CytoplasmicSequence analysisAdd BLAST18
Transmembranei951 – 972HelicalSequence analysisAdd BLAST22
Topological domaini973 – 982LumenalSequence analysis10
Transmembranei983 – 1004HelicalSequence analysisAdd BLAST22
Topological domaini1005 – 1030CytoplasmicSequence analysisAdd BLAST26

GO - Cellular componenti

  • chloroplast Source: TAIR
  • integral component of plasma membrane Source: GO_Central
  • plant-type vacuole Source: TAIR
  • plant-type vacuole membrane Source: TAIR
  • vacuolar membrane Source: TAIR
  • vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000464121 – 1030Calcium-transporting ATPase 4, plasma membrane-typeAdd BLAST1030

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei28PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO22218.
PRIDEiO22218.

PTM databases

iPTMnetiO22218.

Expressioni

Gene expression databases

GenevisibleiO22218. AT.

Interactioni

GO - Molecular functioni

  • calmodulin binding Source: TAIR

Protein-protein interaction databases

BioGridi4091. 1 interactor.
STRINGi3702.AT2G41560.1.

Structurei

3D structure databases

ProteinModelPortaliO22218.
SMRiO22218.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 30Interaction with calmodulinAdd BLAST12

Domaini

The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
HOGENOMiHOG000265623.
InParanoidiO22218.
KOiK01537.
OMAiGARAFCK.
OrthoDBiEOG09360106.
PhylomeDBiO22218.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR024750. Ca_ATPase_N_dom.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF12515. CaATP_NAI. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O22218-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNLLRDFEV EAKNPSLEAR QRWRSSVSIV KNRTRRFRNI RDLDKLADYE
60 70 80 90 100
NKKHQIQEKI RVAFFVQKAA LHFIDAAARP EYKLTDEVKK AGFSIEADEL
110 120 130 140 150
ASMVRKNDTK SLAQKGGVEE LAKKVSVSLS EGIRSSEVPI REKIFGENRY
160 170 180 190 200
TEKPARSFLM FVWEALHDIT LIILMVCAVV SIGVGVATEG FPRGMYDGTG
210 220 230 240 250
ILLSILLVVM VTAISDYKQS LQFRDLDREK KKIIVQVTRD GSRQEISIHD
260 270 280 290 300
LVVGDVVHLS IGDQVPADGI FISGYNLEID ESSLSGESEP SHVNKEKPFL
310 320 330 340 350
LSGTKVQNGS AKMLVTTVGM RTEWGKLMET LVDGGEDETP LQVKLNGVAT
360 370 380 390 400
IIGKIGLSFA VLTFVVLCIR FVLDKATSGS FTNWSSEDAL TLLDYFAISV
410 420 430 440 450
TIIVVAVPEG LPLAVTLSLA FAMKKLMSDR ALVRHLAACE TMGSSTCICT
460 470 480 490 500
DKTGTLTTNH MVVNKVWICD KVQERQEGSK ESFELELSEE VQSTLLQGIF
510 520 530 540 550
QNTGSEVVKD KDGNTQILGS PTERAILEFG LLLGGDFNTQ RKEHKILKIE
560 570 580 590 600
PFNSDKKKMS VLIALPGGGA RAFCKGASEI VLKMCENVVD SNGESVPLTE
610 620 630 640 650
ERITSISDII EGFASEALRT LCLVYKDLDE APSGELPDGG YTMVAVVGIK
660 670 680 690 700
DPVRPGVREA VQTCQAAGIT VRMVTGDNIS TAKAIAKECG IYTEGGLAIE
710 720 730 740 750
GSEFRDLSPH EMRAIIPKIQ VMARSLPLDK HTLVSNLRKI GEVVAVTGDG
760 770 780 790 800
TNDAPALHEA DIGLAMGIAG TEVAKENADV IIMDDNFKTI VNVARWGRAV
810 820 830 840 850
YINIQKFVQF QLTVNVVALI INFVSACITG SAPLTAVQLL WVNMIMDTLG
860 870 880 890 900
ALALATEPPN EGLMKRAPIA RTASFITKTM WRNIAGQSVY QLIVLGILNF
910 920 930 940 950
AGKSLLKLDG PDSTAVLNTV IFNSFVFCQV FNEINSREIE KINVFKGMFN
960 970 980 990 1000
SWVFTWVMTV TVVFQVIIVE FLGAFASTVP LSWQHWLLSI LIGSLNMIVA
1010 1020 1030
VILKCVPVES RHHHDGYDLL PSGPSSSNSA
Length:1,030
Mass (Da):112,749
Last modified:January 1, 1998 - v1
Checksum:iBD31D923E6AE2D13
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF200739 mRNA. Translation: AAG35585.1.
AC002510 Genomic DNA. Translation: AAB84338.1.
CP002685 Genomic DNA. Translation: AEC10000.1.
PIRiT00812.
RefSeqiNP_181687.1. NM_129719.4.
UniGeneiAt.24960.
At.67009.

Genome annotation databases

EnsemblPlantsiAT2G41560.1; AT2G41560.1; AT2G41560.
GeneIDi818754.
GrameneiAT2G41560.1; AT2G41560.1; AT2G41560.
KEGGiath:AT2G41560.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF200739 mRNA. Translation: AAG35585.1.
AC002510 Genomic DNA. Translation: AAB84338.1.
CP002685 Genomic DNA. Translation: AEC10000.1.
PIRiT00812.
RefSeqiNP_181687.1. NM_129719.4.
UniGeneiAt.24960.
At.67009.

3D structure databases

ProteinModelPortaliO22218.
SMRiO22218.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4091. 1 interactor.
STRINGi3702.AT2G41560.1.

PTM databases

iPTMnetiO22218.

Proteomic databases

PaxDbiO22218.
PRIDEiO22218.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G41560.1; AT2G41560.1; AT2G41560.
GeneIDi818754.
GrameneiAT2G41560.1; AT2G41560.1; AT2G41560.
KEGGiath:AT2G41560.

Organism-specific databases

TAIRiAT2G41560.

Phylogenomic databases

eggNOGiKOG0204. Eukaryota.
ENOG410XNNC. LUCA.
HOGENOMiHOG000265623.
InParanoidiO22218.
KOiK01537.
OMAiGARAFCK.
OrthoDBiEOG09360106.
PhylomeDBiO22218.

Enzyme and pathway databases

BioCyciARA:AT2G41560-MONOMER.
ReactomeiR-ATH-418359. Reduction of cytosolic Ca++ levels.
R-ATH-5578775. Ion homeostasis.
R-ATH-936837. Ion transport by P-type ATPases.

Miscellaneous databases

PROiO22218.

Gene expression databases

GenevisibleiO22218. AT.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR024750. Ca_ATPase_N_dom.
IPR023214. HAD-like_dom.
IPR006408. P-type_ATPase_IIB.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF12515. CaATP_NAI. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACA4_ARATH
AccessioniPrimary (citable) accession number: O22218
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 152 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.