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O22218

- ACA4_ARATH

UniProt

O22218 - ACA4_ARATH

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Protein

Calcium-transporting ATPase 4, plasma membrane-type

Gene

ACA4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into small vacuoles.

Catalytic activityi

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Enzyme regulationi

Activated by calmodulin.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei451 – 45114-aspartylphosphate intermediateBy similarity
Metal bindingi749 – 7491MagnesiumBy similarity
Metal bindingi753 – 7531MagnesiumBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. calcium-transporting ATPase activity Source: TAIR
  3. calmodulin binding Source: TAIR
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. anion homeostasis Source: TAIR
  2. defense response to bacterium Source: TAIR
  3. negative regulation of programmed cell death Source: TAIR
  4. response to nematode Source: TAIR
  5. response to osmotic stress Source: TAIR
  6. response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Calcium transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G41560-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-transporting ATPase 4, plasma membrane-type (EC:3.6.3.8)
Alternative name(s):
Ca(2+)-ATPase isoform 4
Gene namesi
Name:ACA4
Ordered Locus Names:At2g41560
ORF Names:T32G6.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G41560.

Subcellular locationi

Vacuole membrane; Multi-pass membrane protein
Note: Tonoplast. Small vacuoles.

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. integral component of membrane Source: UniProtKB-KW
  3. plant-type vacuole Source: TAIR
  4. plant-type vacuole membrane Source: TAIR
  5. vacuolar membrane Source: TAIR
  6. vacuole Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10301030Calcium-transporting ATPase 4, plasma membrane-typePRO_0000046412Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei28 – 281Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO22218.
PRIDEiO22218.

Expressioni

Gene expression databases

ExpressionAtlasiO22218. baseline and differential.
GenevestigatoriO22218.

Interactioni

Protein-protein interaction databases

BioGridi4091. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliO22218.
SMRiO22218. Positions 113-1007.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 157157CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini179 – 19618LumenalSequence AnalysisAdd
BLAST
Topological domaini218 – 345128CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini366 – 39530LumenalSequence AnalysisAdd
BLAST
Topological domaini414 – 804391CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini824 – 83411LumenalSequence AnalysisAdd
BLAST
Topological domaini856 – 87520CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini899 – 91012LumenalSequence AnalysisAdd
BLAST
Topological domaini933 – 95018CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini973 – 98210LumenalSequence Analysis
Topological domaini1005 – 103026CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei158 – 17821HelicalSequence AnalysisAdd
BLAST
Transmembranei197 – 21721HelicalSequence AnalysisAdd
BLAST
Transmembranei346 – 36520HelicalSequence AnalysisAdd
BLAST
Transmembranei396 – 41318HelicalSequence AnalysisAdd
BLAST
Transmembranei805 – 82319HelicalSequence AnalysisAdd
BLAST
Transmembranei835 – 85521HelicalSequence AnalysisAdd
BLAST
Transmembranei876 – 89823HelicalSequence AnalysisAdd
BLAST
Transmembranei911 – 93222HelicalSequence AnalysisAdd
BLAST
Transmembranei951 – 97222HelicalSequence AnalysisAdd
BLAST
Transmembranei983 – 100422HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni19 – 3012Interaction with calmodulinAdd
BLAST

Domaini

The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
HOGENOMiHOG000265623.
InParanoidiO22218.
KOiK01537.
OMAiGARAFCK.
PhylomeDBiO22218.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006408. ATPase_P-typ_Ca-transp_plasma.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR024750. Ca_ATPase_N_dom.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF12515. CaATP_NAI. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
PR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O22218-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNLLRDFEV EAKNPSLEAR QRWRSSVSIV KNRTRRFRNI RDLDKLADYE
60 70 80 90 100
NKKHQIQEKI RVAFFVQKAA LHFIDAAARP EYKLTDEVKK AGFSIEADEL
110 120 130 140 150
ASMVRKNDTK SLAQKGGVEE LAKKVSVSLS EGIRSSEVPI REKIFGENRY
160 170 180 190 200
TEKPARSFLM FVWEALHDIT LIILMVCAVV SIGVGVATEG FPRGMYDGTG
210 220 230 240 250
ILLSILLVVM VTAISDYKQS LQFRDLDREK KKIIVQVTRD GSRQEISIHD
260 270 280 290 300
LVVGDVVHLS IGDQVPADGI FISGYNLEID ESSLSGESEP SHVNKEKPFL
310 320 330 340 350
LSGTKVQNGS AKMLVTTVGM RTEWGKLMET LVDGGEDETP LQVKLNGVAT
360 370 380 390 400
IIGKIGLSFA VLTFVVLCIR FVLDKATSGS FTNWSSEDAL TLLDYFAISV
410 420 430 440 450
TIIVVAVPEG LPLAVTLSLA FAMKKLMSDR ALVRHLAACE TMGSSTCICT
460 470 480 490 500
DKTGTLTTNH MVVNKVWICD KVQERQEGSK ESFELELSEE VQSTLLQGIF
510 520 530 540 550
QNTGSEVVKD KDGNTQILGS PTERAILEFG LLLGGDFNTQ RKEHKILKIE
560 570 580 590 600
PFNSDKKKMS VLIALPGGGA RAFCKGASEI VLKMCENVVD SNGESVPLTE
610 620 630 640 650
ERITSISDII EGFASEALRT LCLVYKDLDE APSGELPDGG YTMVAVVGIK
660 670 680 690 700
DPVRPGVREA VQTCQAAGIT VRMVTGDNIS TAKAIAKECG IYTEGGLAIE
710 720 730 740 750
GSEFRDLSPH EMRAIIPKIQ VMARSLPLDK HTLVSNLRKI GEVVAVTGDG
760 770 780 790 800
TNDAPALHEA DIGLAMGIAG TEVAKENADV IIMDDNFKTI VNVARWGRAV
810 820 830 840 850
YINIQKFVQF QLTVNVVALI INFVSACITG SAPLTAVQLL WVNMIMDTLG
860 870 880 890 900
ALALATEPPN EGLMKRAPIA RTASFITKTM WRNIAGQSVY QLIVLGILNF
910 920 930 940 950
AGKSLLKLDG PDSTAVLNTV IFNSFVFCQV FNEINSREIE KINVFKGMFN
960 970 980 990 1000
SWVFTWVMTV TVVFQVIIVE FLGAFASTVP LSWQHWLLSI LIGSLNMIVA
1010 1020 1030
VILKCVPVES RHHHDGYDLL PSGPSSSNSA
Length:1,030
Mass (Da):112,749
Last modified:January 1, 1998 - v1
Checksum:iBD31D923E6AE2D13
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF200739 mRNA. Translation: AAG35585.1.
AC002510 Genomic DNA. Translation: AAB84338.1.
CP002685 Genomic DNA. Translation: AEC10000.1.
PIRiT00812.
RefSeqiNP_181687.1. NM_129719.3.
UniGeneiAt.24960.
At.67009.

Genome annotation databases

EnsemblPlantsiAT2G41560.1; AT2G41560.1; AT2G41560.
GeneIDi818754.
KEGGiath:AT2G41560.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF200739 mRNA. Translation: AAG35585.1 .
AC002510 Genomic DNA. Translation: AAB84338.1 .
CP002685 Genomic DNA. Translation: AEC10000.1 .
PIRi T00812.
RefSeqi NP_181687.1. NM_129719.3.
UniGenei At.24960.
At.67009.

3D structure databases

ProteinModelPortali O22218.
SMRi O22218. Positions 113-1007.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 4091. 1 interaction.

Proteomic databases

PaxDbi O22218.
PRIDEi O22218.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G41560.1 ; AT2G41560.1 ; AT2G41560 .
GeneIDi 818754.
KEGGi ath:AT2G41560.

Organism-specific databases

TAIRi AT2G41560.

Phylogenomic databases

eggNOGi COG0474.
HOGENOMi HOG000265623.
InParanoidi O22218.
KOi K01537.
OMAi GARAFCK.
PhylomeDBi O22218.

Enzyme and pathway databases

BioCyci ARA:AT2G41560-MONOMER.

Miscellaneous databases

PROi O22218.

Gene expression databases

ExpressionAtlasi O22218. baseline and differential.
Genevestigatori O22218.

Family and domain databases

Gene3Di 1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProi IPR006408. ATPase_P-typ_Ca-transp_plasma.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR024750. Ca_ATPase_N_dom.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF12515. CaATP_NAI. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
PR00120. HATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The ACA4 gene of Arabidopsis encodes a vacuolar membrane calcium pump that improves salt tolerance in yeast."
    Geisler M., Frangne N., Gomes E., Martinoia E., Palmgren M.G.
    Plant Physiol. 124:1814-1827(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. Erratum
    Geisler M., Frangne N., Gomes E., Martinoia E., Palmgren M.G.
    Plant Physiol. 126:1341-1342(2001)
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  4. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  5. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-28, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiACA4_ARATH
AccessioniPrimary (citable) accession number: O22218
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 1, 1998
Last modified: October 29, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3