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O22218

- ACA4_ARATH

UniProt

O22218 - ACA4_ARATH

Protein

Calcium-transporting ATPase 4, plasma membrane-type

Gene

ACA4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into small vacuoles.

    Catalytic activityi

    ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

    Enzyme regulationi

    Activated by calmodulin.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei451 – 45114-aspartylphosphate intermediateBy similarity
    Metal bindingi749 – 7491MagnesiumBy similarity
    Metal bindingi753 – 7531MagnesiumBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. calcium-transporting ATPase activity Source: TAIR
    3. calmodulin binding Source: TAIR
    4. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. anion homeostasis Source: TAIR
    2. defense response to bacterium Source: TAIR
    3. negative regulation of programmed cell death Source: TAIR
    4. response to nematode Source: TAIR
    5. response to osmotic stress Source: TAIR
    6. response to salt stress Source: TAIR

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Calcium transport, Ion transport, Transport

    Keywords - Ligandi

    ATP-binding, Calcium, Calmodulin-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G41560-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Calcium-transporting ATPase 4, plasma membrane-type (EC:3.6.3.8)
    Alternative name(s):
    Ca(2+)-ATPase isoform 4
    Gene namesi
    Name:ACA4
    Ordered Locus Names:At2g41560
    ORF Names:T32G6.8
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G41560.

    Subcellular locationi

    Vacuole membrane; Multi-pass membrane protein
    Note: Tonoplast. Small vacuoles.

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. integral component of membrane Source: UniProtKB-KW
    3. plant-type vacuole Source: TAIR
    4. plant-type vacuole membrane Source: TAIR
    5. vacuolar membrane Source: TAIR
    6. vacuole Source: TAIR

    Keywords - Cellular componenti

    Membrane, Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10301030Calcium-transporting ATPase 4, plasma membrane-typePRO_0000046412Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei28 – 281Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiO22218.
    PRIDEiO22218.

    Expressioni

    Gene expression databases

    ArrayExpressiO22218.
    GenevestigatoriO22218.

    Interactioni

    Protein-protein interaction databases

    BioGridi4091. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliO22218.
    SMRiO22218. Positions 113-1007.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 157157CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini179 – 19618LumenalSequence AnalysisAdd
    BLAST
    Topological domaini218 – 345128CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini366 – 39530LumenalSequence AnalysisAdd
    BLAST
    Topological domaini414 – 804391CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini824 – 83411LumenalSequence AnalysisAdd
    BLAST
    Topological domaini856 – 87520CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini899 – 91012LumenalSequence AnalysisAdd
    BLAST
    Topological domaini933 – 95018CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini973 – 98210LumenalSequence Analysis
    Topological domaini1005 – 103026CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei158 – 17821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei197 – 21721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei346 – 36520HelicalSequence AnalysisAdd
    BLAST
    Transmembranei396 – 41318HelicalSequence AnalysisAdd
    BLAST
    Transmembranei805 – 82319HelicalSequence AnalysisAdd
    BLAST
    Transmembranei835 – 85521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei876 – 89823HelicalSequence AnalysisAdd
    BLAST
    Transmembranei911 – 93222HelicalSequence AnalysisAdd
    BLAST
    Transmembranei951 – 97222HelicalSequence AnalysisAdd
    BLAST
    Transmembranei983 – 100422HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni19 – 3012Interaction with calmodulinAdd
    BLAST

    Domaini

    The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0474.
    HOGENOMiHOG000265623.
    InParanoidiO22218.
    KOiK01537.
    OMAiGARAFCK.
    PhylomeDBiO22218.

    Family and domain databases

    Gene3Di1.20.1110.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProiIPR006408. ATPase_P-typ_Ca-transp_plasma.
    IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR024750. Ca_ATPase_N_dom.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view]
    PfamiPF12515. CaATP_NAI. 1 hit.
    PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    PR00120. HATPASE.
    SMARTiSM00831. Cation_ATPase_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsiTIGR01517. ATPase-IIB_Ca. 1 hit.
    TIGR01494. ATPase_P-type. 3 hits.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O22218-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNLLRDFEV EAKNPSLEAR QRWRSSVSIV KNRTRRFRNI RDLDKLADYE     50
    NKKHQIQEKI RVAFFVQKAA LHFIDAAARP EYKLTDEVKK AGFSIEADEL 100
    ASMVRKNDTK SLAQKGGVEE LAKKVSVSLS EGIRSSEVPI REKIFGENRY 150
    TEKPARSFLM FVWEALHDIT LIILMVCAVV SIGVGVATEG FPRGMYDGTG 200
    ILLSILLVVM VTAISDYKQS LQFRDLDREK KKIIVQVTRD GSRQEISIHD 250
    LVVGDVVHLS IGDQVPADGI FISGYNLEID ESSLSGESEP SHVNKEKPFL 300
    LSGTKVQNGS AKMLVTTVGM RTEWGKLMET LVDGGEDETP LQVKLNGVAT 350
    IIGKIGLSFA VLTFVVLCIR FVLDKATSGS FTNWSSEDAL TLLDYFAISV 400
    TIIVVAVPEG LPLAVTLSLA FAMKKLMSDR ALVRHLAACE TMGSSTCICT 450
    DKTGTLTTNH MVVNKVWICD KVQERQEGSK ESFELELSEE VQSTLLQGIF 500
    QNTGSEVVKD KDGNTQILGS PTERAILEFG LLLGGDFNTQ RKEHKILKIE 550
    PFNSDKKKMS VLIALPGGGA RAFCKGASEI VLKMCENVVD SNGESVPLTE 600
    ERITSISDII EGFASEALRT LCLVYKDLDE APSGELPDGG YTMVAVVGIK 650
    DPVRPGVREA VQTCQAAGIT VRMVTGDNIS TAKAIAKECG IYTEGGLAIE 700
    GSEFRDLSPH EMRAIIPKIQ VMARSLPLDK HTLVSNLRKI GEVVAVTGDG 750
    TNDAPALHEA DIGLAMGIAG TEVAKENADV IIMDDNFKTI VNVARWGRAV 800
    YINIQKFVQF QLTVNVVALI INFVSACITG SAPLTAVQLL WVNMIMDTLG 850
    ALALATEPPN EGLMKRAPIA RTASFITKTM WRNIAGQSVY QLIVLGILNF 900
    AGKSLLKLDG PDSTAVLNTV IFNSFVFCQV FNEINSREIE KINVFKGMFN 950
    SWVFTWVMTV TVVFQVIIVE FLGAFASTVP LSWQHWLLSI LIGSLNMIVA 1000
    VILKCVPVES RHHHDGYDLL PSGPSSSNSA 1030
    Length:1,030
    Mass (Da):112,749
    Last modified:January 1, 1998 - v1
    Checksum:iBD31D923E6AE2D13
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF200739 mRNA. Translation: AAG35585.1.
    AC002510 Genomic DNA. Translation: AAB84338.1.
    CP002685 Genomic DNA. Translation: AEC10000.1.
    PIRiT00812.
    RefSeqiNP_181687.1. NM_129719.3.
    UniGeneiAt.24960.
    At.67009.

    Genome annotation databases

    EnsemblPlantsiAT2G41560.1; AT2G41560.1; AT2G41560.
    GeneIDi818754.
    KEGGiath:AT2G41560.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF200739 mRNA. Translation: AAG35585.1 .
    AC002510 Genomic DNA. Translation: AAB84338.1 .
    CP002685 Genomic DNA. Translation: AEC10000.1 .
    PIRi T00812.
    RefSeqi NP_181687.1. NM_129719.3.
    UniGenei At.24960.
    At.67009.

    3D structure databases

    ProteinModelPortali O22218.
    SMRi O22218. Positions 113-1007.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 4091. 1 interaction.

    Proteomic databases

    PaxDbi O22218.
    PRIDEi O22218.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G41560.1 ; AT2G41560.1 ; AT2G41560 .
    GeneIDi 818754.
    KEGGi ath:AT2G41560.

    Organism-specific databases

    TAIRi AT2G41560.

    Phylogenomic databases

    eggNOGi COG0474.
    HOGENOMi HOG000265623.
    InParanoidi O22218.
    KOi K01537.
    OMAi GARAFCK.
    PhylomeDBi O22218.

    Enzyme and pathway databases

    BioCyci ARA:AT2G41560-MONOMER.

    Miscellaneous databases

    PROi O22218.

    Gene expression databases

    ArrayExpressi O22218.
    Genevestigatori O22218.

    Family and domain databases

    Gene3Di 1.20.1110.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProi IPR006408. ATPase_P-typ_Ca-transp_plasma.
    IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR024750. Ca_ATPase_N_dom.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view ]
    Pfami PF12515. CaATP_NAI. 1 hit.
    PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    PR00120. HATPASE.
    SMARTi SM00831. Cation_ATPase_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 2 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsi TIGR01517. ATPase-IIB_Ca. 1 hit.
    TIGR01494. ATPase_P-type. 3 hits.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The ACA4 gene of Arabidopsis encodes a vacuolar membrane calcium pump that improves salt tolerance in yeast."
      Geisler M., Frangne N., Gomes E., Martinoia E., Palmgren M.G.
      Plant Physiol. 124:1814-1827(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. Erratum
      Geisler M., Frangne N., Gomes E., Martinoia E., Palmgren M.G.
      Plant Physiol. 126:1341-1342(2001)
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    4. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    5. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
      Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
      Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-28, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiACA4_ARATH
    AccessioniPrimary (citable) accession number: O22218
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 11, 2001
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 136 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3