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O22218 (ACA4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 135. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calcium-transporting ATPase 4, plasma membrane-type

EC=3.6.3.8
Alternative name(s):
Ca(2+)-ATPase isoform 4
Gene names
Name:ACA4
Ordered Locus Names:At2g41560
ORF Names:T32G6.8
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length1030 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into small vacuoles.

Catalytic activity

ATP + H2O + Ca2+(Side 1) = ADP + phosphate + Ca2+(Side 2).

Enzyme regulation

Activated by calmodulin.

Subcellular location

Vacuole membrane; Multi-pass membrane protein. Note: Tonoplast. Small vacuoles.

Domain

The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.

Sequence similarities

Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. [View classification]

Ontologies

Keywords
   Biological processCalcium transport
Ion transport
Transport
   Cellular componentMembrane
Vacuole
   DomainTransmembrane
Transmembrane helix
   LigandATP-binding
Calcium
Calmodulin-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionHydrolase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processanion homeostasis

Inferred from mutant phenotype PubMed 20837703. Source: TAIR

defense response to bacterium

Inferred from genetic interaction PubMed 20837703. Source: TAIR

negative regulation of programmed cell death

Inferred from genetic interaction PubMed 20837703. Source: TAIR

response to nematode

Inferred from expression pattern PubMed 16478044. Source: TAIR

response to osmotic stress

Inferred from genetic interaction Ref.1. Source: TAIR

response to salt stress

Inferred from genetic interaction Ref.1. Source: TAIR

   Cellular_componentchloroplast

Inferred from direct assay PubMed 18431481. Source: TAIR

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plant-type vacuole

Inferred from direct assay Ref.1PubMed 16618929. Source: TAIR

plant-type vacuole membrane

Inferred from direct assay PubMed 20837703. Source: TAIR

vacuolar membrane

Inferred from direct assay PubMed 16618929PubMed 17151019. Source: TAIR

vacuole

Inferred from direct assay PubMed 15539469PubMed 22923678. Source: TAIR

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

calcium-transporting ATPase activity

Inferred from sequence or structural similarity PubMed 12805592. Source: TAIR

calmodulin binding

Inferred from direct assay Ref.1. Source: TAIR

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10301030Calcium-transporting ATPase 4, plasma membrane-type
PRO_0000046412

Regions

Topological domain1 – 157157Cytoplasmic Potential
Transmembrane158 – 17821Helical; Potential
Topological domain179 – 19618Lumenal Potential
Transmembrane197 – 21721Helical; Potential
Topological domain218 – 345128Cytoplasmic Potential
Transmembrane346 – 36520Helical; Potential
Topological domain366 – 39530Lumenal Potential
Transmembrane396 – 41318Helical; Potential
Topological domain414 – 804391Cytoplasmic Potential
Transmembrane805 – 82319Helical; Potential
Topological domain824 – 83411Lumenal Potential
Transmembrane835 – 85521Helical; Potential
Topological domain856 – 87520Cytoplasmic Potential
Transmembrane876 – 89823Helical; Potential
Topological domain899 – 91012Lumenal Potential
Transmembrane911 – 93222Helical; Potential
Topological domain933 – 95018Cytoplasmic Potential
Transmembrane951 – 97222Helical; Potential
Topological domain973 – 98210Lumenal Potential
Transmembrane983 – 100422Helical; Potential
Topological domain1005 – 103026Cytoplasmic Potential
Region19 – 3012Interaction with calmodulin

Sites

Active site45114-aspartylphosphate intermediate By similarity
Metal binding7491Magnesium By similarity
Metal binding7531Magnesium By similarity

Amino acid modifications

Modified residue281Phosphoserine Ref.5

Sequences

Sequence LengthMass (Da)Tools
O22218 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: BD31D923E6AE2D13

FASTA1,030112,749
        10         20         30         40         50         60 
MSNLLRDFEV EAKNPSLEAR QRWRSSVSIV KNRTRRFRNI RDLDKLADYE NKKHQIQEKI 

        70         80         90        100        110        120 
RVAFFVQKAA LHFIDAAARP EYKLTDEVKK AGFSIEADEL ASMVRKNDTK SLAQKGGVEE 

       130        140        150        160        170        180 
LAKKVSVSLS EGIRSSEVPI REKIFGENRY TEKPARSFLM FVWEALHDIT LIILMVCAVV 

       190        200        210        220        230        240 
SIGVGVATEG FPRGMYDGTG ILLSILLVVM VTAISDYKQS LQFRDLDREK KKIIVQVTRD 

       250        260        270        280        290        300 
GSRQEISIHD LVVGDVVHLS IGDQVPADGI FISGYNLEID ESSLSGESEP SHVNKEKPFL 

       310        320        330        340        350        360 
LSGTKVQNGS AKMLVTTVGM RTEWGKLMET LVDGGEDETP LQVKLNGVAT IIGKIGLSFA 

       370        380        390        400        410        420 
VLTFVVLCIR FVLDKATSGS FTNWSSEDAL TLLDYFAISV TIIVVAVPEG LPLAVTLSLA 

       430        440        450        460        470        480 
FAMKKLMSDR ALVRHLAACE TMGSSTCICT DKTGTLTTNH MVVNKVWICD KVQERQEGSK 

       490        500        510        520        530        540 
ESFELELSEE VQSTLLQGIF QNTGSEVVKD KDGNTQILGS PTERAILEFG LLLGGDFNTQ 

       550        560        570        580        590        600 
RKEHKILKIE PFNSDKKKMS VLIALPGGGA RAFCKGASEI VLKMCENVVD SNGESVPLTE 

       610        620        630        640        650        660 
ERITSISDII EGFASEALRT LCLVYKDLDE APSGELPDGG YTMVAVVGIK DPVRPGVREA 

       670        680        690        700        710        720 
VQTCQAAGIT VRMVTGDNIS TAKAIAKECG IYTEGGLAIE GSEFRDLSPH EMRAIIPKIQ 

       730        740        750        760        770        780 
VMARSLPLDK HTLVSNLRKI GEVVAVTGDG TNDAPALHEA DIGLAMGIAG TEVAKENADV 

       790        800        810        820        830        840 
IIMDDNFKTI VNVARWGRAV YINIQKFVQF QLTVNVVALI INFVSACITG SAPLTAVQLL 

       850        860        870        880        890        900 
WVNMIMDTLG ALALATEPPN EGLMKRAPIA RTASFITKTM WRNIAGQSVY QLIVLGILNF 

       910        920        930        940        950        960 
AGKSLLKLDG PDSTAVLNTV IFNSFVFCQV FNEINSREIE KINVFKGMFN SWVFTWVMTV 

       970        980        990       1000       1010       1020 
TVVFQVIIVE FLGAFASTVP LSWQHWLLSI LIGSLNMIVA VILKCVPVES RHHHDGYDLL 

      1030 
PSGPSSSNSA 

« Hide

References

« Hide 'large scale' references
[1]"The ACA4 gene of Arabidopsis encodes a vacuolar membrane calcium pump that improves salt tolerance in yeast."
Geisler M., Frangne N., Gomes E., Martinoia E., Palmgren M.G.
Plant Physiol. 124:1814-1827(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]Erratum
Geisler M., Frangne N., Gomes E., Martinoia E., Palmgren M.G.
Plant Physiol. 126:1341-1342(2001)
[3]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[4]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[5]"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-28, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF200739 mRNA. Translation: AAG35585.1.
AC002510 Genomic DNA. Translation: AAB84338.1.
CP002685 Genomic DNA. Translation: AEC10000.1.
PIRT00812.
RefSeqNP_181687.1. NM_129719.3.
UniGeneAt.24960.
At.67009.

3D structure databases

ProteinModelPortalO22218.
SMRO22218. Positions 113-1007.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid4091. 1 interaction.

Proteomic databases

PaxDbO22218.
PRIDEO22218.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G41560.1; AT2G41560.1; AT2G41560.
GeneID818754.
KEGGath:AT2G41560.

Organism-specific databases

TAIRAT2G41560.

Phylogenomic databases

eggNOGCOG0474.
HOGENOMHOG000265623.
InParanoidO22218.
KOK01537.
OMAGARAFCK.
PhylomeDBO22218.

Enzyme and pathway databases

BioCycARA:AT2G41560-MONOMER.

Gene expression databases

ArrayExpressO22218.
GenevestigatorO22218.

Family and domain databases

Gene3D1.20.1110.10. 2 hits.
3.40.1110.10. 1 hit.
InterProIPR006408. ATPase_P-typ_Ca-transp_plasma.
IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR024750. Ca_ATPase_N_dom.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamPF12515. CaATP_NAI. 1 hit.
PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSPR00119. CATATPASE.
PR00120. HATPASE.
SMARTSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMSSF56784. SSF56784. 2 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsTIGR01517. ATPase-IIB_Ca. 1 hit.
TIGR01494. ATPase_P-type. 3 hits.
PROSITEPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROO22218.

Entry information

Entry nameACA4_ARATH
AccessionPrimary (citable) accession number: O22218
Entry history
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: January 1, 1998
Last modified: June 11, 2014
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names