Calcium-transporting ATPase 4, plasma membrane-type - Arabidopsis thaliana (Mouse-ear cress)

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Reviewed, UniProtKB/Swiss-Prot O22218 (ACA4_ARATH)

Last modified November 3, 2009. Version 93. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Calcium-transporting ATPase 4, plasma membrane-type
    EC=3.6.3.8
Alternative name(s):
    Ca(2+)-ATPase isoform 4

Gene names

Name:	ACA4
Ordered Locus Names:	At2g41560
ORF Names:	T32G6.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

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Protein attributes

Sequence length	1030 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into small vacuoles.
Catalytic activity	ATP + H₂O + Ca²⁺(Cis) = ADP + phosphate + Ca²⁺(Trans).
Enzyme regulation	Activated by calmodulin.
Subcellular location	Vacuole membrane; Multi-pass membrane protein. Note: Tonoplast. Small vacuoles.
Domain	The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IIB subfamily.

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Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Membrane Vacuole
Domain	Transmembrane
Ligand	ATP-binding Calcium Calmodulin-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro calcium ion transport Inferred from electronic annotation. Source: UniProtKB-KW response to nematode Inferred from expression pattern. Source: TAIR response to salt stress Ref.1 Inferred from genetic interaction. Source: TAIR
Cellular component	chloroplast Inferred from direct assay. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plant-type vacuole Ref.1 Inferred from direct assay. Source: TAIR plasma membrane Inferred from direct assay. Source: TAIR vacuolar membrane Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calcium-transporting ATPase activity Inferred from electronic annotation. Source: EC calmodulin binding Ref.1 Inferred from direct assay. Source: TAIR magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1030

1030

Calcium-transporting ATPase 4, plasma membrane-type

PRO_0000046412

Regions

Topological domain

1 – 157

157

Cytoplasmic Potential

Transmembrane

158 – 178

Potential

Topological domain

179 – 196

Lumenal Potential

Transmembrane

197 – 217

Potential

Topological domain

218 – 345

128

Cytoplasmic Potential

Transmembrane

346 – 365

Potential

Topological domain

366 – 395

Lumenal Potential

Transmembrane

396 – 413

Potential

Topological domain

414 – 804

391

Cytoplasmic Potential

Transmembrane

805 – 823

Potential

Topological domain

824 – 834

Lumenal Potential

Transmembrane

835 – 855

Potential

Topological domain

856 – 875

Cytoplasmic Potential

Transmembrane

876 – 898

Potential

Topological domain

899 – 910

Lumenal Potential

Transmembrane

911 – 932

Potential

Topological domain

933 – 950

Cytoplasmic Potential

Transmembrane

951 – 972

Potential

Topological domain

973 – 982

Lumenal Potential

Transmembrane

983 – 1004

Potential

Topological domain

1005 – 1030

Cytoplasmic Potential

Region

19 – 30

Interaction with calmodulin

Sites

Active site

451

4-aspartylphosphate intermediate By similarity

Metal binding

749

Magnesium By similarity

Metal binding

753

Magnesium By similarity

Amino acid modifications

Modified residue

Phosphoserine Ref.4

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Sequences

Sequence

Length

Mass (Da)

Tools

O22218-1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: BD31D923E6AE2D13
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FASTA

1,030

112,749

        10         20         30         40         50         60 
MSNLLRDFEV EAKNPSLEAR QRWRSSVSIV KNRTRRFRNI RDLDKLADYE NKKHQIQEKI 

        70         80         90        100        110        120 
RVAFFVQKAA LHFIDAAARP EYKLTDEVKK AGFSIEADEL ASMVRKNDTK SLAQKGGVEE 

       130        140        150        160        170        180 
LAKKVSVSLS EGIRSSEVPI REKIFGENRY TEKPARSFLM FVWEALHDIT LIILMVCAVV 

       190        200        210        220        230        240 
SIGVGVATEG FPRGMYDGTG ILLSILLVVM VTAISDYKQS LQFRDLDREK KKIIVQVTRD 

       250        260        270        280        290        300 
GSRQEISIHD LVVGDVVHLS IGDQVPADGI FISGYNLEID ESSLSGESEP SHVNKEKPFL 

       310        320        330        340        350        360 
LSGTKVQNGS AKMLVTTVGM RTEWGKLMET LVDGGEDETP LQVKLNGVAT IIGKIGLSFA 

       370        380        390        400        410        420 
VLTFVVLCIR FVLDKATSGS FTNWSSEDAL TLLDYFAISV TIIVVAVPEG LPLAVTLSLA 

       430        440        450        460        470        480 
FAMKKLMSDR ALVRHLAACE TMGSSTCICT DKTGTLTTNH MVVNKVWICD KVQERQEGSK 

       490        500        510        520        530        540 
ESFELELSEE VQSTLLQGIF QNTGSEVVKD KDGNTQILGS PTERAILEFG LLLGGDFNTQ 

       550        560        570        580        590        600 
RKEHKILKIE PFNSDKKKMS VLIALPGGGA RAFCKGASEI VLKMCENVVD SNGESVPLTE 

       610        620        630        640        650        660 
ERITSISDII EGFASEALRT LCLVYKDLDE APSGELPDGG YTMVAVVGIK DPVRPGVREA 

       670        680        690        700        710        720 
VQTCQAAGIT VRMVTGDNIS TAKAIAKECG IYTEGGLAIE GSEFRDLSPH EMRAIIPKIQ 

       730        740        750        760        770        780 
VMARSLPLDK HTLVSNLRKI GEVVAVTGDG TNDAPALHEA DIGLAMGIAG TEVAKENADV 

       790        800        810        820        830        840 
IIMDDNFKTI VNVARWGRAV YINIQKFVQF QLTVNVVALI INFVSACITG SAPLTAVQLL 

       850        860        870        880        890        900 
WVNMIMDTLG ALALATEPPN EGLMKRAPIA RTASFITKTM WRNIAGQSVY QLIVLGILNF 

       910        920        930        940        950        960 
AGKSLLKLDG PDSTAVLNTV IFNSFVFCQV FNEINSREIE KINVFKGMFN SWVFTWVMTV 

       970        980        990       1000       1010       1020 
TVVFQVIIVE FLGAFASTVP LSWQHWLLSI LIGSLNMIVA VILKCVPVES RHHHDGYDLL 

      1030 
PSGPSSSNSA

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The ACA4 gene of Arabidopsis encodes a vacuolar membrane calcium pump that improves salt tolerance in yeast." Geisler M., Frangne N., Gomes E., Martinoia E., Palmgren M.G. Plant Physiol. 124:1814-1827(2000) [PubMed: 11115896] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	Erratum Geisler M., Frangne N., Gomes E., Martinoia E., Palmgren M.G. Plant Physiol. 126:1341-1342(2001)
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed: 19376835] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-28, MASS SPECTROMETRY. Tissue: Seedling.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	AF200739 mRNA. Translation: AAG35585.1. AC002510 Genomic DNA. Translation: AAB84338.1.
IPI	IPI00546719.
PIR	T00812.
RefSeq	NP_181687.1.
UniGene	At.24960 At.67009
3D structure databases
HSSP	HSSP built from PDB template 1EUL based on UniProtKB P04191.
ModBase	Search...
Proteomic databases
PRIDE	O22218.
Genome annotation databases
GeneID	818754.
GenomeReviews	Gene locus AT2G41560 in contig CT485783_GR.
KEGG	ath:AT2G41560.
NMPDR	fig\|3702.1.peg.11296.
Organism-specific databases
TAIR	At2g41560.
Phylogenomic databases
OMA	LNTVIFN.
Enzyme and pathway databases
BRENDA	3.6.3.8. 302.
Gene expression databases
ArrayExpress	O22218.
Genevestigator	O22218.
GermOnline	AT2G41560. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-reg. IPR006408. ATPase_P-typ_Ca-transp_PMCA. IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR000695. ATPase_P-typ_H-transp. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR005834. Dehalogen-like_hydro. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE. PR00120. HATPASE.
TIGRFAMs	TIGR01517. ATPase-IIB_Ca. 1 hit. TIGR01494. ATPase_P-type. 4 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

ACA4_ARATH

Accession

Primary (citable) accession number: O22218

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 1, 1998
Last modified:	November 3, 2009
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)

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Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents