Calcium-transporting ATPase 4, plasma membrane-type - Arabidopsis thaliana (Mouse-ear cress)

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O22218 (ACA4_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 124. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Calcium-transporting ATPase 4, plasma membrane-type
EC=3.6.3.8

Alternative name(s):
Ca(2+)-ATPase isoform 4

Gene names

Name:	ACA4
Ordered Locus Names:	At2g41560
ORF Names:	T32G6.8

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1030 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into small vacuoles.
Catalytic activity	ATP + H₂O + Ca²⁺(Side 1) = ADP + phosphate + Ca²⁺(Side 2).
Enzyme regulation	Activated by calmodulin.
Subcellular location	Vacuole membrane; Multi-pass membrane protein. Note: Tonoplast. Small vacuoles.
Domain	The N-terminus contains an autoinhibitory calmodulin-binding domain, which binds calmodulin in a calcium-dependent fashion.
Sequence similarities	Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIB subfamily. [View classification]

Ontologies

Keywords
Biological process	Calcium transport Ion transport Transport
Cellular component	Membrane Vacuole
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Calcium Calmodulin-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	anion homeostasis Inferred from mutant phenotype PubMed 20837703. Source: TAIR defense response to bacterium Inferred from genetic interaction PubMed 20837703. Source: TAIR negative regulation of programmed cell death Inferred from genetic interaction PubMed 20837703. Source: TAIR response to nematode Inferred from expression pattern PubMed 16478044. Source: TAIR response to salt stress Inferred from genetic interaction Ref.1. Source: TAIR
Cellular_component	chloroplast Inferred from direct assay PubMed 18431481. Source: TAIR integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plant-type vacuole membrane Inferred from direct assay PubMed 20837703. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calcium-transporting ATPase activity Inferred from sequence or structural similarity PubMed 12805592. Source: TAIR calmodulin binding Inferred from direct assay Ref.1. Source: TAIR metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1030

1030

Calcium-transporting ATPase 4, plasma membrane-type

PRO_0000046412

Regions

Topological domain

1 – 157

157

Cytoplasmic Potential

Transmembrane

158 – 178

Helical; Potential

Topological domain

179 – 196

Lumenal Potential

Transmembrane

197 – 217

Helical; Potential

Topological domain

218 – 345

128

Cytoplasmic Potential

Transmembrane

346 – 365

Helical; Potential

Topological domain

366 – 395

Lumenal Potential

Transmembrane

396 – 413

Helical; Potential

Topological domain

414 – 804

391

Cytoplasmic Potential

Transmembrane

805 – 823

Helical; Potential

Topological domain

824 – 834

Lumenal Potential

Transmembrane

835 – 855

Helical; Potential

Topological domain

856 – 875

Cytoplasmic Potential

Transmembrane

876 – 898

Helical; Potential

Topological domain

899 – 910

Lumenal Potential

Transmembrane

911 – 932

Helical; Potential

Topological domain

933 – 950

Cytoplasmic Potential

Transmembrane

951 – 972

Helical; Potential

Topological domain

973 – 982

Lumenal Potential

Transmembrane

983 – 1004

Helical; Potential

Topological domain

1005 – 1030

Cytoplasmic Potential

Region

19 – 30

Interaction with calmodulin

Sites

Active site

451

4-aspartylphosphate intermediate By similarity

Metal binding

749

Magnesium By similarity

Metal binding

753

Magnesium By similarity

Amino acid modifications

Modified residue

Phosphoserine Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

O22218 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: BD31D923E6AE2D13
Show »

FASTA

1,030

112,749

        10         20         30         40         50         60 
MSNLLRDFEV EAKNPSLEAR QRWRSSVSIV KNRTRRFRNI RDLDKLADYE NKKHQIQEKI 

        70         80         90        100        110        120 
RVAFFVQKAA LHFIDAAARP EYKLTDEVKK AGFSIEADEL ASMVRKNDTK SLAQKGGVEE 

       130        140        150        160        170        180 
LAKKVSVSLS EGIRSSEVPI REKIFGENRY TEKPARSFLM FVWEALHDIT LIILMVCAVV 

       190        200        210        220        230        240 
SIGVGVATEG FPRGMYDGTG ILLSILLVVM VTAISDYKQS LQFRDLDREK KKIIVQVTRD 

       250        260        270        280        290        300 
GSRQEISIHD LVVGDVVHLS IGDQVPADGI FISGYNLEID ESSLSGESEP SHVNKEKPFL 

       310        320        330        340        350        360 
LSGTKVQNGS AKMLVTTVGM RTEWGKLMET LVDGGEDETP LQVKLNGVAT IIGKIGLSFA 

       370        380        390        400        410        420 
VLTFVVLCIR FVLDKATSGS FTNWSSEDAL TLLDYFAISV TIIVVAVPEG LPLAVTLSLA 

       430        440        450        460        470        480 
FAMKKLMSDR ALVRHLAACE TMGSSTCICT DKTGTLTTNH MVVNKVWICD KVQERQEGSK 

       490        500        510        520        530        540 
ESFELELSEE VQSTLLQGIF QNTGSEVVKD KDGNTQILGS PTERAILEFG LLLGGDFNTQ 

       550        560        570        580        590        600 
RKEHKILKIE PFNSDKKKMS VLIALPGGGA RAFCKGASEI VLKMCENVVD SNGESVPLTE 

       610        620        630        640        650        660 
ERITSISDII EGFASEALRT LCLVYKDLDE APSGELPDGG YTMVAVVGIK DPVRPGVREA 

       670        680        690        700        710        720 
VQTCQAAGIT VRMVTGDNIS TAKAIAKECG IYTEGGLAIE GSEFRDLSPH EMRAIIPKIQ 

       730        740        750        760        770        780 
VMARSLPLDK HTLVSNLRKI GEVVAVTGDG TNDAPALHEA DIGLAMGIAG TEVAKENADV 

       790        800        810        820        830        840 
IIMDDNFKTI VNVARWGRAV YINIQKFVQF QLTVNVVALI INFVSACITG SAPLTAVQLL 

       850        860        870        880        890        900 
WVNMIMDTLG ALALATEPPN EGLMKRAPIA RTASFITKTM WRNIAGQSVY QLIVLGILNF 

       910        920        930        940        950        960 
AGKSLLKLDG PDSTAVLNTV IFNSFVFCQV FNEINSREIE KINVFKGMFN SWVFTWVMTV 

       970        980        990       1000       1010       1020 
TVVFQVIIVE FLGAFASTVP LSWQHWLLSI LIGSLNMIVA VILKCVPVES RHHHDGYDLL 

      1030 
PSGPSSSNSA

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The ACA4 gene of Arabidopsis encodes a vacuolar membrane calcium pump that improves salt tolerance in yeast." Geisler M., Frangne N., Gomes E., Martinoia E., Palmgren M.G. Plant Physiol. 124:1814-1827(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	Erratum Geisler M., Frangne N., Gomes E., Martinoia E., Palmgren M.G. Plant Physiol. 126:1341-1342(2001)
[3]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	"Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks." Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S. Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-28, MASS SPECTROMETRY. Strain: cv. Columbia. Tissue: Seedling.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF200739 mRNA. Translation: AAG35585.1. AC002510 Genomic DNA. Translation: AAB84338.1. CP002685 Genomic DNA. Translation: AEC10000.1.
IPI	IPI00546719.
PIR	T00812.
RefSeq	NP_181687.1. NM_129719.3.
UniGene	At.24960. At.67009.
3D structure databases
ProteinModelPortal	O22218.
SMR	O22218. Positions 15-75, 113-1007.
ModBase	Search...
Proteomic databases
PaxDb	O22218.
PRIDE	O22218.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G41560.1; AT2G41560.1; AT2G41560.
GeneID	818754.
KEGG	ath:AT2G41560.
Organism-specific databases
TAIR	At2g41560.
Phylogenomic databases
eggNOG	COG0474.
HOGENOM	HOG000265623.
InParanoid	O22218.
KO	K01537.
OMA	RFRNIRD.
PhylomeDB	O22218.
ProtClustDB	CLSN2683782.
Gene expression databases
ArrayExpress	O22218.
Genevestigator	O22218.
GermOnline	AT2G41560. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.20.1110.10. 2 hits. 3.40.1110.10. 1 hit.
InterPro	IPR006408. ATPase_P-typ_Ca-transp_plasma. IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR023299. ATPase_P-typ_cyto_domN. IPR018303. ATPase_P-typ_P_site. IPR023298. ATPase_P-typ_TM_dom. IPR008250. ATPase_P-typ_transduc_dom_A. IPR024750. Ca_ATPase_N_dom. IPR001757. Cation_transp_P_typ_ATPase. IPR023214. HAD-like_dom. [Graphical view]
PANTHER	PTHR24093. PTHR24093. 1 hit.
Pfam	PF12515. CaATP_NAI. 1 hit. PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE. PR00120. HATPASE.
SMART	SM00831. Cation_ATPase_N. 1 hit. [Graphical view]
SUPFAM	SSF81660. ATPase_cation_domN. 1 hit. SSF56784. HAD-like_dom. 1 hit.
TIGRFAMs	TIGR01517. ATPase-IIB_Ca. 1 hit. TIGR01494. ATPase_P-type. 3 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACA4_ARATH

Accession

Primary (citable) accession number: O22218

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	January 1, 1998
Last modified:	May 1, 2013
This is version 124 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents