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O22216

- GPDHC_ARATH

UniProt

O22216 - GPDHC_ARATH

Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic

Gene

GPDHC1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Involved in cell redox homeostasis. Required for maintaining a steady state cellular NADH/NAD+ ratio through a mitochondrial glycerol-3-phosphate redox shuttle. May function with the mitochondrial FAD-dependent glycerol-3-phosphate dehydrogenase SDP6 to shuttle reducing equivalents into the mitochondria for respiration.1 Publication

    Catalytic activityi

    sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei196 – 1961NAD; via amide nitrogenBy similarity
    Binding sitei196 – 1961SubstrateBy similarity
    Binding sitei235 – 2351NAD; via amide nitrogenBy similarity
    Active sitei285 – 2851Proton acceptorBy similarity
    Binding sitei347 – 3471NADBy similarity
    Binding sitei375 – 3751NADBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi48 – 536NADBy similarity

    GO - Molecular functioni

    1. glycerol-3-phosphate dehydrogenase [NAD+] activity Source: TAIR
    2. NAD binding Source: TAIR

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. glycerol-3-phosphate catabolic process Source: InterPro
    3. response to stress Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Stress response

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciARA:AT2G41540-MONOMER.
    ARA:GQT-489-MONOMER.
    ARA:GQT-490-MONOMER.
    BRENDAi1.1.1.8. 302.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic (EC:1.1.1.8)
    Gene namesi
    Name:GPDHC1
    Ordered Locus Names:At2g41540
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G41540.

    Subcellular locationi

    Cytoplasmcytosol By similarity

    GO - Cellular componenti

    1. cytosol Source: TAIR
    2. glycerol-3-phosphate dehydrogenase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype under normal growth conditions, but mutant plants have increased NADH/NAD+ ratios, decreased levels of glycerol-3-phosphate, and produce constitutive high levels of reactive oxygen species (ROS).1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 462462Glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolicPRO_0000420176Add
    BLAST

    Proteomic databases

    PRIDEiO22216.

    Expressioni

    Tissue specificityi

    Expressed in roots, leaves, flowers and siliques.1 Publication

    Inductioni

    By abscisic acid and salt and dehydration treatments. Down-regulated by hypoxia.1 Publication

    Gene expression databases

    GenevestigatoriO22216.

    Interactioni

    Protein-protein interaction databases

    STRINGi3702.AT2G41540.3-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO22216.
    SMRiO22216. Positions 42-419.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni347 – 3482Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    InParanoidiO22216.
    OMAiPTENILY.
    PhylomeDBiO22216.

    Family and domain databases

    Gene3Di1.10.1040.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR006168. G3P_DH_NAD-dep.
    IPR006109. G3P_DH_NAD-dep_C.
    IPR011128. G3P_DH_NAD-dep_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11728. PTHR11728. 1 hit.
    PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
    PF01210. NAD_Gly3P_dh_N. 1 hit.
    [Graphical view]
    PRINTSiPR00077. GPDHDRGNASE.
    SUPFAMiSSF48179. SSF48179. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O22216-1 [UniParc]FASTAAdd to Basket

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    MVGSIEAKSL QSNGSVHHIG LNLEEKLDEF RRLLGKSEKD PLRIVSVGAG    50
    AWGSVFAALL QESYGGFRDK FQIRIWRRAG RAVDRETAEH LFEVINSRED 100
    ILRRLIRRCA YLKYVEARLG DRTLYADEIL KDGFCLNMVD TPLCPLKVVT 150
    NLQEAVWDAD IVVNGLPSTE TREVFEEISK YWKERITVPI IISLSKGIET 200
    ALEPVPHIIT PTKMIHQATG VPIDNVLYLG GPNIAAEIYN KEYANARICG 250
    AAKWRKPLAK FLRQPHFIVW DNSDLVTHEV MGGLKNVYAI GAGMVAALTN 300
    ESATSKSVYF AHCTSEMIFI THLLAEEPEK LAGPLLADTY VTLLKGRNAW 350
    YGQMLAKGEI NRDMGDSISG KGMIQGVSAV GAFYQLLSQS SLSILPSEEK 400
    KPVAPVESCP ILKTLYKILI TREQSTQAIL QALRDETLND PRDRIEIAQS 450
    HAFYRPSLLG QP 462
    Length:462
    Mass (Da):51,491
    Last modified:January 1, 1998 - v1
    Checksum:iAD40AF1F464C4908
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti316 – 3161E → G in BAH20028. (PubMed:19423640)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ347019 mRNA. Translation: CAC69665.1.
    AC002510 Genomic DNA. Translation: AAB84336.1.
    CP002685 Genomic DNA. Translation: AEC09996.1.
    CP002685 Genomic DNA. Translation: AEC09997.1.
    CP002685 Genomic DNA. Translation: AEC09998.1.
    AY063920 mRNA. Translation: AAL36276.1.
    AY091247 mRNA. Translation: AAM14186.1.
    AK317356 mRNA. Translation: BAH20028.1.
    PIRiT00810.
    RefSeqiNP_001031525.1. NM_001036448.2.
    NP_181685.1. NM_129717.4.
    NP_850352.1. NM_180021.4.
    UniGeneiAt.21264.
    At.69847.

    Genome annotation databases

    EnsemblPlantsiAT2G41540.1; AT2G41540.1; AT2G41540.
    AT2G41540.2; AT2G41540.2; AT2G41540.
    AT2G41540.3; AT2G41540.3; AT2G41540.
    GeneIDi818752.
    KEGGiath:AT2G41540.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ347019 mRNA. Translation: CAC69665.1 .
    AC002510 Genomic DNA. Translation: AAB84336.1 .
    CP002685 Genomic DNA. Translation: AEC09996.1 .
    CP002685 Genomic DNA. Translation: AEC09997.1 .
    CP002685 Genomic DNA. Translation: AEC09998.1 .
    AY063920 mRNA. Translation: AAL36276.1 .
    AY091247 mRNA. Translation: AAM14186.1 .
    AK317356 mRNA. Translation: BAH20028.1 .
    PIRi T00810.
    RefSeqi NP_001031525.1. NM_001036448.2.
    NP_181685.1. NM_129717.4.
    NP_850352.1. NM_180021.4.
    UniGenei At.21264.
    At.69847.

    3D structure databases

    ProteinModelPortali O22216.
    SMRi O22216. Positions 42-419.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G41540.3-P.

    Proteomic databases

    PRIDEi O22216.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G41540.1 ; AT2G41540.1 ; AT2G41540 .
    AT2G41540.2 ; AT2G41540.2 ; AT2G41540 .
    AT2G41540.3 ; AT2G41540.3 ; AT2G41540 .
    GeneIDi 818752.
    KEGGi ath:AT2G41540.

    Organism-specific databases

    TAIRi AT2G41540.

    Phylogenomic databases

    InParanoidi O22216.
    OMAi PTENILY.
    PhylomeDBi O22216.

    Enzyme and pathway databases

    BioCyci ARA:AT2G41540-MONOMER.
    ARA:GQT-489-MONOMER.
    ARA:GQT-490-MONOMER.
    BRENDAi 1.1.1.8. 302.

    Gene expression databases

    Genevestigatori O22216.

    Family and domain databases

    Gene3Di 1.10.1040.10. 1 hit.
    3.40.50.720. 1 hit.
    InterProi IPR008927. 6-PGluconate_DH_C-like.
    IPR013328. DH_multihelical.
    IPR006168. G3P_DH_NAD-dep.
    IPR006109. G3P_DH_NAD-dep_C.
    IPR011128. G3P_DH_NAD-dep_N.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11728. PTHR11728. 1 hit.
    Pfami PF07479. NAD_Gly3P_dh_C. 1 hit.
    PF01210. NAD_Gly3P_dh_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00077. GPDHDRGNASE.
    SUPFAMi SSF48179. SSF48179. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Involvement of a glycerol-3-phosphate dehydrogenase in modulating the NADH/NAD+ ratio provides evidence of a mitochondrial glycerol-3-phosphate shuttle in Arabidopsis."
      Shen W., Wei Y., Dauk M., Tan Y., Taylor D.C., Selvaraj G., Zou J.
      Plant Cell 18:422-441(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
      Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
      DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
      Tissue: Rosette leaf.

    Entry informationi

    Entry nameiGPDHC_ARATH
    AccessioniPrimary (citable) accession number: O22216
    Secondary accession number(s): B9DH11
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 28, 2012
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3