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Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic

Gene

GPDHC1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in cell redox homeostasis. Required for maintaining a steady state cellular NADH/NAD+ ratio through a mitochondrial glycerol-3-phosphate redox shuttle. May function with the mitochondrial FAD-dependent glycerol-3-phosphate dehydrogenase SDP6 to shuttle reducing equivalents into the mitochondria for respiration.1 Publication

Catalytic activityi

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei196NAD; via amide nitrogenBy similarity1
Binding sitei196SubstrateBy similarity1
Binding sitei235NAD; via amide nitrogenBy similarity1
Active sitei285Proton acceptorBy similarity1
Binding sitei347NADBy similarity1
Binding sitei375NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi48 – 53NADBy similarity6

GO - Molecular functioni

  • glycerol-3-phosphate dehydrogenase [NAD+] activity Source: UniProtKB-EC
  • NAD binding Source: TAIR

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processStress response
LigandNAD

Enzyme and pathway databases

BioCyciARA:AT2G41540-MONOMER
BRENDAi1.1.1.8 399

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic (EC:1.1.1.8)
Gene namesi
Name:GPDHC1
Ordered Locus Names:At2g41540
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G41540
TAIRilocus:2062734 AT2G41540

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant plants have increased NADH/NAD+ ratios, decreased levels of glycerol-3-phosphate, and produce constitutive high levels of reactive oxygen species (ROS).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004201761 – 462Glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolicAdd BLAST462

Proteomic databases

PaxDbiO22216

Expressioni

Tissue specificityi

Expressed in roots, leaves, flowers and siliques.1 Publication

Inductioni

By abscisic acid and salt and dehydration treatments. Down-regulated by hypoxia.1 Publication

Gene expression databases

ExpressionAtlasiO22216 baseline and differential
GenevisibleiO22216 AT

Interactioni

Protein-protein interaction databases

BioGridi4089, 3 interactors
STRINGi3702.AT2G41540.1

Structurei

3D structure databases

ProteinModelPortaliO22216
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni347 – 348Substrate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2711 Eukaryota
COG0240 LUCA
HOGENOMiHOG000238366
InParanoidiO22216
KOiK00006
OMAiRICGAAK
OrthoDBiEOG093609YK
PhylomeDBiO22216

Family and domain databases

Gene3Di1.10.1040.10, 1 hit
InterProiView protein in InterPro
IPR008927 6-PGluconate_DH-like_C_sf
IPR013328 6PGD_dom2
IPR006168 G3P_DH_NAD-dep
IPR006109 G3P_DH_NAD-dep_C
IPR011128 G3P_DH_NAD-dep_N
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF07479 NAD_Gly3P_dh_C, 1 hit
PF01210 NAD_Gly3P_dh_N, 1 hit
PRINTSiPR00077 GPDHDRGNASE
SUPFAMiSSF48179 SSF48179, 1 hit
SSF51735 SSF51735, 2 hits

Sequencei

Sequence statusi: Complete.

O22216-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGSIEAKSL QSNGSVHHIG LNLEEKLDEF RRLLGKSEKD PLRIVSVGAG
60 70 80 90 100
AWGSVFAALL QESYGGFRDK FQIRIWRRAG RAVDRETAEH LFEVINSRED
110 120 130 140 150
ILRRLIRRCA YLKYVEARLG DRTLYADEIL KDGFCLNMVD TPLCPLKVVT
160 170 180 190 200
NLQEAVWDAD IVVNGLPSTE TREVFEEISK YWKERITVPI IISLSKGIET
210 220 230 240 250
ALEPVPHIIT PTKMIHQATG VPIDNVLYLG GPNIAAEIYN KEYANARICG
260 270 280 290 300
AAKWRKPLAK FLRQPHFIVW DNSDLVTHEV MGGLKNVYAI GAGMVAALTN
310 320 330 340 350
ESATSKSVYF AHCTSEMIFI THLLAEEPEK LAGPLLADTY VTLLKGRNAW
360 370 380 390 400
YGQMLAKGEI NRDMGDSISG KGMIQGVSAV GAFYQLLSQS SLSILPSEEK
410 420 430 440 450
KPVAPVESCP ILKTLYKILI TREQSTQAIL QALRDETLND PRDRIEIAQS
460
HAFYRPSLLG QP
Length:462
Mass (Da):51,491
Last modified:January 1, 1998 - v1
Checksum:iAD40AF1F464C4908
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti316E → G in BAH20028 (PubMed:19423640).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ347019 mRNA Translation: CAC69665.1
AC002510 Genomic DNA Translation: AAB84336.1
CP002685 Genomic DNA Translation: AEC09996.1
CP002685 Genomic DNA Translation: AEC09997.1
CP002685 Genomic DNA Translation: AEC09998.1
CP002685 Genomic DNA Translation: ANM63134.1
AY063920 mRNA Translation: AAL36276.1
AY091247 mRNA Translation: AAM14186.1
AK317356 mRNA Translation: BAH20028.1
PIRiT00810
RefSeqiNP_001031525.1, NM_001036448.2
NP_001325243.1, NM_001336924.1
NP_181685.1, NM_129717.5
NP_850352.1, NM_180021.5
UniGeneiAt.21264
At.69847

Genome annotation databases

EnsemblPlantsiAT2G41540.1; AT2G41540.1; AT2G41540
AT2G41540.2; AT2G41540.2; AT2G41540
AT2G41540.3; AT2G41540.3; AT2G41540
AT2G41540.4; AT2G41540.4; AT2G41540
GeneIDi818752
GrameneiAT2G41540.1; AT2G41540.1; AT2G41540
AT2G41540.2; AT2G41540.2; AT2G41540
AT2G41540.3; AT2G41540.3; AT2G41540
AT2G41540.4; AT2G41540.4; AT2G41540
KEGGiath:AT2G41540

Similar proteinsi

Entry informationi

Entry nameiGPDHC_ARATH
AccessioniPrimary (citable) accession number: O22216
Secondary accession number(s): B9DH11
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 135 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families
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