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Protein

Glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic

Gene

GPDHC1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in cell redox homeostasis. Required for maintaining a steady state cellular NADH/NAD+ ratio through a mitochondrial glycerol-3-phosphate redox shuttle. May function with the mitochondrial FAD-dependent glycerol-3-phosphate dehydrogenase SDP6 to shuttle reducing equivalents into the mitochondria for respiration.1 Publication

Catalytic activityi

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei196 – 1961NAD; via amide nitrogenBy similarity
Binding sitei196 – 1961SubstrateBy similarity
Binding sitei235 – 2351NAD; via amide nitrogenBy similarity
Active sitei285 – 2851Proton acceptorBy similarity
Binding sitei347 – 3471NADBy similarity
Binding sitei375 – 3751NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi48 – 536NADBy similarity

GO - Molecular functioni

  1. glycerol-3-phosphate dehydrogenase [NAD+] activity Source: TAIR
  2. NAD binding Source: TAIR

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. glycerol-3-phosphate catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Stress response

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciARA:AT2G41540-MONOMER.
ARA:GQT-489-MONOMER.
ARA:GQT-490-MONOMER.
BRENDAi1.1.1.8. 302.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic (EC:1.1.1.8)
Gene namesi
Name:GPDHC1
Ordered Locus Names:At2g41540
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G41540.

Subcellular locationi

Cytoplasmcytosol By similarity

GO - Cellular componenti

  1. cytosol Source: TAIR
  2. glycerol-3-phosphate dehydrogenase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant plants have increased NADH/NAD+ ratios, decreased levels of glycerol-3-phosphate, and produce constitutive high levels of reactive oxygen species (ROS).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462Glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolicPRO_0000420176Add
BLAST

Proteomic databases

PRIDEiO22216.

Expressioni

Tissue specificityi

Expressed in roots, leaves, flowers and siliques.1 Publication

Inductioni

By abscisic acid and salt and dehydration treatments. Down-regulated by hypoxia.1 Publication

Gene expression databases

GenevestigatoriO22216.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G41540.3-P.

Structurei

3D structure databases

ProteinModelPortaliO22216.
SMRiO22216. Positions 42-419.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni347 – 3482Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

InParanoidiO22216.
OMAiTHAFFEL.
PhylomeDBiO22216.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11728. PTHR11728. 1 hit.
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.

Sequencei

Sequence statusi: Complete.

O22216-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVGSIEAKSL QSNGSVHHIG LNLEEKLDEF RRLLGKSEKD PLRIVSVGAG
60 70 80 90 100
AWGSVFAALL QESYGGFRDK FQIRIWRRAG RAVDRETAEH LFEVINSRED
110 120 130 140 150
ILRRLIRRCA YLKYVEARLG DRTLYADEIL KDGFCLNMVD TPLCPLKVVT
160 170 180 190 200
NLQEAVWDAD IVVNGLPSTE TREVFEEISK YWKERITVPI IISLSKGIET
210 220 230 240 250
ALEPVPHIIT PTKMIHQATG VPIDNVLYLG GPNIAAEIYN KEYANARICG
260 270 280 290 300
AAKWRKPLAK FLRQPHFIVW DNSDLVTHEV MGGLKNVYAI GAGMVAALTN
310 320 330 340 350
ESATSKSVYF AHCTSEMIFI THLLAEEPEK LAGPLLADTY VTLLKGRNAW
360 370 380 390 400
YGQMLAKGEI NRDMGDSISG KGMIQGVSAV GAFYQLLSQS SLSILPSEEK
410 420 430 440 450
KPVAPVESCP ILKTLYKILI TREQSTQAIL QALRDETLND PRDRIEIAQS
460
HAFYRPSLLG QP
Length:462
Mass (Da):51,491
Last modified:January 1, 1998 - v1
Checksum:iAD40AF1F464C4908
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti316 – 3161E → G in BAH20028 (PubMed:19423640).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ347019 mRNA. Translation: CAC69665.1.
AC002510 Genomic DNA. Translation: AAB84336.1.
CP002685 Genomic DNA. Translation: AEC09996.1.
CP002685 Genomic DNA. Translation: AEC09997.1.
CP002685 Genomic DNA. Translation: AEC09998.1.
AY063920 mRNA. Translation: AAL36276.1.
AY091247 mRNA. Translation: AAM14186.1.
AK317356 mRNA. Translation: BAH20028.1.
PIRiT00810.
RefSeqiNP_001031525.1. NM_001036448.2.
NP_181685.1. NM_129717.4.
NP_850352.1. NM_180021.4.
UniGeneiAt.21264.
At.69847.

Genome annotation databases

EnsemblPlantsiAT2G41540.1; AT2G41540.1; AT2G41540.
AT2G41540.2; AT2G41540.2; AT2G41540.
AT2G41540.3; AT2G41540.3; AT2G41540.
GeneIDi818752.
KEGGiath:AT2G41540.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ347019 mRNA. Translation: CAC69665.1.
AC002510 Genomic DNA. Translation: AAB84336.1.
CP002685 Genomic DNA. Translation: AEC09996.1.
CP002685 Genomic DNA. Translation: AEC09997.1.
CP002685 Genomic DNA. Translation: AEC09998.1.
AY063920 mRNA. Translation: AAL36276.1.
AY091247 mRNA. Translation: AAM14186.1.
AK317356 mRNA. Translation: BAH20028.1.
PIRiT00810.
RefSeqiNP_001031525.1. NM_001036448.2.
NP_181685.1. NM_129717.4.
NP_850352.1. NM_180021.4.
UniGeneiAt.21264.
At.69847.

3D structure databases

ProteinModelPortaliO22216.
SMRiO22216. Positions 42-419.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G41540.3-P.

Proteomic databases

PRIDEiO22216.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G41540.1; AT2G41540.1; AT2G41540.
AT2G41540.2; AT2G41540.2; AT2G41540.
AT2G41540.3; AT2G41540.3; AT2G41540.
GeneIDi818752.
KEGGiath:AT2G41540.

Organism-specific databases

TAIRiAT2G41540.

Phylogenomic databases

InParanoidiO22216.
OMAiTHAFFEL.
PhylomeDBiO22216.

Enzyme and pathway databases

BioCyciARA:AT2G41540-MONOMER.
ARA:GQT-489-MONOMER.
ARA:GQT-490-MONOMER.
BRENDAi1.1.1.8. 302.

Gene expression databases

GenevestigatoriO22216.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11728. PTHR11728. 1 hit.
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Involvement of a glycerol-3-phosphate dehydrogenase in modulating the NADH/NAD+ ratio provides evidence of a mitochondrial glycerol-3-phosphate shuttle in Arabidopsis."
    Shen W., Wei Y., Dauk M., Tan Y., Taylor D.C., Selvaraj G., Zou J.
    Plant Cell 18:422-441(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
    Tissue: Rosette leaf.

Entry informationi

Entry nameiGPDHC_ARATH
AccessioniPrimary (citable) accession number: O22216
Secondary accession number(s): B9DH11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: January 1, 1998
Last modified: January 7, 2015
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.