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O22216 (GPDHC_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic

EC=1.1.1.8
Gene names
Name:GPDHC1
Ordered Locus Names:At2g41540
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length462 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in cell redox homeostasis. Required for maintaining a steady state cellular NADH/NAD+ ratio through a mitochondrial glycerol-3-phosphate redox shuttle. May function with the mitochondrial FAD-dependent glycerol-3-phosphate dehydrogenase SDP6 to shuttle reducing equivalents into the mitochondria for respiration. Ref.1

Catalytic activity

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Subcellular location

Cytoplasmcytosol By similarity.

Tissue specificity

Expressed in roots, leaves, flowers and siliques. Ref.1

Induction

By abscisic acid and salt and dehydration treatments. Down-regulated by hypoxia. Ref.1

Disruption phenotype

No visible phenotype under normal growth conditions, but mutant plants have increased NADH/NAD+ ratios, decreased levels of glycerol-3-phosphate, and produce constitutive high levels of reactive oxygen species (ROS). Ref.1

Sequence similarities

Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 462462Glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic
PRO_0000420176

Regions

Nucleotide binding48 – 536NAD By similarity
Region347 – 3482Substrate binding By similarity

Sites

Active site2851Proton acceptor By similarity
Binding site1961NAD; via amide nitrogen By similarity
Binding site1961Substrate By similarity
Binding site2351NAD; via amide nitrogen By similarity
Binding site3471NAD By similarity
Binding site3751NAD By similarity

Experimental info

Sequence conflict3161E → G in BAH20028. Ref.5

Sequences

Sequence LengthMass (Da)Tools
O22216 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: AD40AF1F464C4908

FASTA46251,491
        10         20         30         40         50         60 
MVGSIEAKSL QSNGSVHHIG LNLEEKLDEF RRLLGKSEKD PLRIVSVGAG AWGSVFAALL 

        70         80         90        100        110        120 
QESYGGFRDK FQIRIWRRAG RAVDRETAEH LFEVINSRED ILRRLIRRCA YLKYVEARLG 

       130        140        150        160        170        180 
DRTLYADEIL KDGFCLNMVD TPLCPLKVVT NLQEAVWDAD IVVNGLPSTE TREVFEEISK 

       190        200        210        220        230        240 
YWKERITVPI IISLSKGIET ALEPVPHIIT PTKMIHQATG VPIDNVLYLG GPNIAAEIYN 

       250        260        270        280        290        300 
KEYANARICG AAKWRKPLAK FLRQPHFIVW DNSDLVTHEV MGGLKNVYAI GAGMVAALTN 

       310        320        330        340        350        360 
ESATSKSVYF AHCTSEMIFI THLLAEEPEK LAGPLLADTY VTLLKGRNAW YGQMLAKGEI 

       370        380        390        400        410        420 
NRDMGDSISG KGMIQGVSAV GAFYQLLSQS SLSILPSEEK KPVAPVESCP ILKTLYKILI 

       430        440        450        460 
TREQSTQAIL QALRDETLND PRDRIEIAQS HAFYRPSLLG QP 

« Hide

References

« Hide 'large scale' references
[1]"Involvement of a glycerol-3-phosphate dehydrogenase in modulating the NADH/NAD+ ratio provides evidence of a mitochondrial glycerol-3-phosphate shuttle in Arabidopsis."
Shen W., Wei Y., Dauk M., Tan Y., Taylor D.C., Selvaraj G., Zou J.
Plant Cell 18:422-441(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
Tissue: Rosette leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ347019 mRNA. Translation: CAC69665.1.
AC002510 Genomic DNA. Translation: AAB84336.1.
CP002685 Genomic DNA. Translation: AEC09996.1.
CP002685 Genomic DNA. Translation: AEC09997.1.
CP002685 Genomic DNA. Translation: AEC09998.1.
AY063920 mRNA. Translation: AAL36276.1.
AY091247 mRNA. Translation: AAM14186.1.
AK317356 mRNA. Translation: BAH20028.1.
PIRT00810.
RefSeqNP_001031525.1. NM_001036448.2.
NP_181685.1. NM_129717.4.
NP_850352.1. NM_180021.4.
UniGeneAt.21264.
At.69847.

3D structure databases

ProteinModelPortalO22216.
SMRO22216. Positions 42-419.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT2G41540.3-P.

Proteomic databases

PRIDEO22216.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G41540.1; AT2G41540.1; AT2G41540.
AT2G41540.2; AT2G41540.2; AT2G41540.
AT2G41540.3; AT2G41540.3; AT2G41540.
GeneID818752.
KEGGath:AT2G41540.

Organism-specific databases

TAIRAT2G41540.

Phylogenomic databases

InParanoidO22216.
OMAPTENILY.
PhylomeDBO22216.
ProtClustDBCLSN2683781.

Enzyme and pathway databases

BioCycARA:AT2G41540-MONOMER.
ARA:GQT-489-MONOMER.
ARA:GQT-490-MONOMER.
BRENDA1.1.1.8. 302.

Gene expression databases

GenevestigatorO22216.

Family and domain databases

Gene3D1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11728. PTHR11728. 1 hit.
PfamPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PRINTSPR00077. GPDHDRGNASE.
SUPFAMSSF48179. SSF48179. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGPDHC_ARATH
AccessionPrimary (citable) accession number: O22216
Secondary accession number(s): B9DH11
Entry history
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: January 1, 1998
Last modified: February 19, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names