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O22216

- GPDHC_ARATH

UniProt

O22216 - GPDHC_ARATH

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Protein
Glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic
Gene
GPDHC1, At2g41540
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Involved in cell redox homeostasis. Required for maintaining a steady state cellular NADH/NAD+ ratio through a mitochondrial glycerol-3-phosphate redox shuttle. May function with the mitochondrial FAD-dependent glycerol-3-phosphate dehydrogenase SDP6 to shuttle reducing equivalents into the mitochondria for respiration.1 Publication

Catalytic activityi

sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei196 – 1961NAD; via amide nitrogen By similarity
Binding sitei196 – 1961Substrate By similarity
Binding sitei235 – 2351NAD; via amide nitrogen By similarity
Active sitei285 – 2851Proton acceptor By similarity
Binding sitei347 – 3471NAD By similarity
Binding sitei375 – 3751NAD By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi48 – 536NAD By similarity

GO - Molecular functioni

  1. NAD binding Source: TAIR
  2. glycerol-3-phosphate dehydrogenase [NAD+] activity Source: TAIR

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. glycerol-3-phosphate catabolic process Source: InterPro
  3. response to stress Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Stress response

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciARA:AT2G41540-MONOMER.
ARA:GQT-489-MONOMER.
ARA:GQT-490-MONOMER.
BRENDAi1.1.1.8. 302.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic (EC:1.1.1.8)
Gene namesi
Name:GPDHC1
Ordered Locus Names:At2g41540
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G41540.

Subcellular locationi

Cytoplasmcytosol By similarity

GO - Cellular componenti

  1. cytosol Source: TAIR
  2. glycerol-3-phosphate dehydrogenase complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

No visible phenotype under normal growth conditions, but mutant plants have increased NADH/NAD+ ratios, decreased levels of glycerol-3-phosphate, and produce constitutive high levels of reactive oxygen species (ROS).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 462462Glycerol-3-phosphate dehydrogenase [NAD(+)] GPDHC1, cytosolic
PRO_0000420176Add
BLAST

Proteomic databases

PRIDEiO22216.

Expressioni

Tissue specificityi

Expressed in roots, leaves, flowers and siliques.1 Publication

Inductioni

By abscisic acid and salt and dehydration treatments. Down-regulated by hypoxia.1 Publication

Gene expression databases

GenevestigatoriO22216.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G41540.3-P.

Structurei

3D structure databases

ProteinModelPortaliO22216.
SMRiO22216. Positions 42-419.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni347 – 3482Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

InParanoidiO22216.
OMAiPTENILY.
PhylomeDBiO22216.

Family and domain databases

Gene3Di1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11728. PTHR11728. 1 hit.
PfamiPF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view]
PRINTSiPR00077. GPDHDRGNASE.
SUPFAMiSSF48179. SSF48179. 1 hit.

Sequencei

Sequence statusi: Complete.

O22216-1 [UniParc]FASTAAdd to Basket

« Hide

MVGSIEAKSL QSNGSVHHIG LNLEEKLDEF RRLLGKSEKD PLRIVSVGAG    50
AWGSVFAALL QESYGGFRDK FQIRIWRRAG RAVDRETAEH LFEVINSRED 100
ILRRLIRRCA YLKYVEARLG DRTLYADEIL KDGFCLNMVD TPLCPLKVVT 150
NLQEAVWDAD IVVNGLPSTE TREVFEEISK YWKERITVPI IISLSKGIET 200
ALEPVPHIIT PTKMIHQATG VPIDNVLYLG GPNIAAEIYN KEYANARICG 250
AAKWRKPLAK FLRQPHFIVW DNSDLVTHEV MGGLKNVYAI GAGMVAALTN 300
ESATSKSVYF AHCTSEMIFI THLLAEEPEK LAGPLLADTY VTLLKGRNAW 350
YGQMLAKGEI NRDMGDSISG KGMIQGVSAV GAFYQLLSQS SLSILPSEEK 400
KPVAPVESCP ILKTLYKILI TREQSTQAIL QALRDETLND PRDRIEIAQS 450
HAFYRPSLLG QP 462
Length:462
Mass (Da):51,491
Last modified:January 1, 1998 - v1
Checksum:iAD40AF1F464C4908
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti316 – 3161E → G in BAH20028. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ347019 mRNA. Translation: CAC69665.1.
AC002510 Genomic DNA. Translation: AAB84336.1.
CP002685 Genomic DNA. Translation: AEC09996.1.
CP002685 Genomic DNA. Translation: AEC09997.1.
CP002685 Genomic DNA. Translation: AEC09998.1.
AY063920 mRNA. Translation: AAL36276.1.
AY091247 mRNA. Translation: AAM14186.1.
AK317356 mRNA. Translation: BAH20028.1.
PIRiT00810.
RefSeqiNP_001031525.1. NM_001036448.2.
NP_181685.1. NM_129717.4.
NP_850352.1. NM_180021.4.
UniGeneiAt.21264.
At.69847.

Genome annotation databases

EnsemblPlantsiAT2G41540.1; AT2G41540.1; AT2G41540.
AT2G41540.2; AT2G41540.2; AT2G41540.
AT2G41540.3; AT2G41540.3; AT2G41540.
GeneIDi818752.
KEGGiath:AT2G41540.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ347019 mRNA. Translation: CAC69665.1 .
AC002510 Genomic DNA. Translation: AAB84336.1 .
CP002685 Genomic DNA. Translation: AEC09996.1 .
CP002685 Genomic DNA. Translation: AEC09997.1 .
CP002685 Genomic DNA. Translation: AEC09998.1 .
AY063920 mRNA. Translation: AAL36276.1 .
AY091247 mRNA. Translation: AAM14186.1 .
AK317356 mRNA. Translation: BAH20028.1 .
PIRi T00810.
RefSeqi NP_001031525.1. NM_001036448.2.
NP_181685.1. NM_129717.4.
NP_850352.1. NM_180021.4.
UniGenei At.21264.
At.69847.

3D structure databases

ProteinModelPortali O22216.
SMRi O22216. Positions 42-419.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT2G41540.3-P.

Proteomic databases

PRIDEi O22216.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G41540.1 ; AT2G41540.1 ; AT2G41540 .
AT2G41540.2 ; AT2G41540.2 ; AT2G41540 .
AT2G41540.3 ; AT2G41540.3 ; AT2G41540 .
GeneIDi 818752.
KEGGi ath:AT2G41540.

Organism-specific databases

TAIRi AT2G41540.

Phylogenomic databases

InParanoidi O22216.
OMAi PTENILY.
PhylomeDBi O22216.

Enzyme and pathway databases

BioCyci ARA:AT2G41540-MONOMER.
ARA:GQT-489-MONOMER.
ARA:GQT-490-MONOMER.
BRENDAi 1.1.1.8. 302.

Gene expression databases

Genevestigatori O22216.

Family and domain databases

Gene3Di 1.10.1040.10. 1 hit.
3.40.50.720. 1 hit.
InterProi IPR008927. 6-PGluconate_DH_C-like.
IPR013328. DH_multihelical.
IPR006168. G3P_DH_NAD-dep.
IPR006109. G3P_DH_NAD-dep_C.
IPR011128. G3P_DH_NAD-dep_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR11728. PTHR11728. 1 hit.
Pfami PF07479. NAD_Gly3P_dh_C. 1 hit.
PF01210. NAD_Gly3P_dh_N. 1 hit.
[Graphical view ]
PRINTSi PR00077. GPDHDRGNASE.
SUPFAMi SSF48179. SSF48179. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Involvement of a glycerol-3-phosphate dehydrogenase in modulating the NADH/NAD+ ratio provides evidence of a mitochondrial glycerol-3-phosphate shuttle in Arabidopsis."
    Shen W., Wei Y., Dauk M., Tan Y., Taylor D.C., Selvaraj G., Zou J.
    Plant Cell 18:422-441(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, INDUCTION, DISRUPTION PHENOTYPE.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs."
    Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K.
    DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
    Tissue: Rosette leaf.

Entry informationi

Entry nameiGPDHC_ARATH
AccessioniPrimary (citable) accession number: O22216
Secondary accession number(s): B9DH11
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2012
Last sequence update: January 1, 1998
Last modified: May 14, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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