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O22207 (UBP5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin carboxyl-terminal hydrolase 5

EC=3.4.19.12
Alternative name(s):
Deubiquitinating enzyme 5
Short name=AtUBP5
Ubiquitin thioesterase 5
Ubiquitin-specific-processing protease 5
Gene names
Name:UBP5
Ordered Locus Names:At2g40930
ORF Names:T20B5.13
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length924 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Ref.1

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sequence similarities

Belongs to the peptidase C19 family.

Contains 1 DUSP domain.

Contains 1 USP domain.

Ontologies

Keywords
   Biological processUbl conjugation pathway
   Molecular functionHydrolase
Protease
Thiol protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processubiquitin-dependent protein catabolic process

Traceable author statement Ref.1. Source: TAIR

   Cellular_componentnucleus

Inferred from sequence or structural similarity Ref.1. Source: TAIR

   Molecular_functionubiquitin-specific protease activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 924924Ubiquitin carboxyl-terminal hydrolase 5
PRO_0000080696

Regions

Domain15 – 145131DUSP
Domain317 – 916600USP

Sites

Active site3261Nucleophile
Active site8741Proton acceptor By similarity

Experimental info

Mutagenesis3261C → S: Loss of activity. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O22207 [UniParc].

Last modified June 1, 2002. Version 2.
Checksum: 0DF2EA93C8D2D51A

FASTA924103,875
        10         20         30         40         50         60 
MAEVSMGSSS SSTDLSPEEE RVFIRDIAIA AEANSKEGDT FYLITQRWWQ EWIEYVNQDQ 

        70         80         90        100        110        120 
PCNTNDGSSL SEHCDSPGSS TLKKPSRIDN SDLIYDSSLE DPSNTSEIIE TLQEGRDYVL 

       130        140        150        160        170        180 
LPQEVWNQLR SWYGGGPTLA RRVISSGLSQ TELAVEVYPL RLQLLLMPKS DHSAIRISKK 

       190        200        210        220        230        240 
ETIRELHRRA CEIFDLDSEH VRIWDYYGHQ KYSLMNDLDK TLDDANLQMD QDILVEVLDI 

       250        260        270        280        290        300 
NGTLSSAHIQ SAQENGLVDG DSTSILIEPS KSSLAAAGGF SSSRNAFRTG SVEVSQSFDN 

       310        320        330        340        350        360 
TYSSTGVTTR GSTAGLTGLL NLGNTCFMNS AIQCLVHTPE FASYFQEDYH QEINWQNPLG 

       370        380        390        400        410        420 
MVGELALAFG DLLRKLWAPG RTPIAPRPFK AKLARFAPQF SGYNQHDSQE LLAFLLDGLH 

       430        440        450        460        470        480 
EDLNRVKHKP YINSRDADGR PDEEVADEFW KNHIARNDSI IVDVCQGQYK STLVCPICNK 

       490        500        510        520        530        540 
VSVTFDPFMY LSLPLQFNTT RAITVTVFSC DKTALPSTIT VNVSKQGRCR DLIQALTNAC 

       550        560        570        580        590        600 
SLKQSEELKL AEIRNNFIHR LFEDPLIPLS SIKDDDHLAA YKLSKSSENT TLLRLVLRRR 

       610        620        630        640        650        660 
DQKAGEREST VQLKPCGTPL LSSASCGDAL TKGKIHCLVQ NMLSPFRREE SVGKKGNSDS 

       670        680        690        700        710        720 
SIPERRSARF NNTEEEDKVG GLKKAKKSNS SDLGASKLSL QLIDEDNKTI NLPDNEAEAM 

       730        740        750        760        770        780 
KLPSSATVTI YLDWTPELSG MYDITCLESL PEVLKYGPTT KKARSEPLSL YACLEAFLRE 

       790        800        810        820        830        840 
EPLVPDEMWF CPQCNERRQA SKKLDLWRLP EVLVIHLKRF SYSRSMKHKL ETFVNFPIHD 

       850        860        870        880        890        900 
LDLTKYVANK NLSQPQLYEL YALTNHYGGM GSGHYTAHIK LLDDSRWYNF DDSHISHINE 

       910        920 
DDVKSGAAYV LFYRRKSDAG GKMT 

« Hide

References

« Hide 'large scale' references
[1]"Ubiquitin-specific proteases from Arabidopsis thaliana: cloning of AtUBP5 and analysis of substrate specificity of AtUBP3, AtUBP4, and AtUBP5 using Escherichia coli in vivo and in vitro assays."
Rao-Naik C., Chandler J.S., McArdle B., Callis J.
Arch. Biochem. Biophys. 379:198-208(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF CYS-326.
Strain: cv. Landsberg erecta.
Tissue: Flower.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[5]"The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF048705 mRNA. Translation: AAF21246.1.
AC002409 Genomic DNA. Translation: AAB86453.2.
CP002685 Genomic DNA. Translation: AEC09900.1.
AY099701 mRNA. Translation: AAM20552.1.
BT000300 mRNA. Translation: AAN15619.1.
PIRT00757.
RefSeqNP_565944.1. NM_129656.3.
UniGeneAt.12368.

3D structure databases

ProteinModelPortalO22207.
SMRO22207. Positions 26-243, 315-525, 737-918.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid4028. 1 interaction.
IntActO22207. 1 interaction.
MINTMINT-8064604.
STRING3702.AT2G40930.1-P.

Protein family/group databases

MEROPSC19.093.

Proteomic databases

PaxDbO22207.
PRIDEO22207.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G40930.1; AT2G40930.1; AT2G40930.
GeneID818691.
KEGGath:AT2G40930.

Organism-specific databases

GeneFarm4913.
TAIRAT2G40930.

Phylogenomic databases

eggNOGCOG5560.
HOGENOMHOG000264375.
InParanoidO22207.
KOK11835.
OMASSLSEHC.
PhylomeDBO22207.

Enzyme and pathway databases

BioCycARA:AT2G40930-MONOMER.

Gene expression databases

GenevestigatorO22207.

Family and domain databases

Gene3D3.30.2230.10. 1 hit.
InterProIPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamPF06337. DUSP. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTSM00695. DUSP. 1 hit.
[Graphical view]
SUPFAMSSF143791. SSF143791. 1 hit.
PROSITEPS51283. DUSP. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameUBP5_ARATH
AccessionPrimary (citable) accession number: O22207
Secondary accession number(s): Q9SEX3
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names