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O22207

- UBP5_ARATH

UniProt

O22207 - UBP5_ARATH

Protein

Ubiquitin carboxyl-terminal hydrolase 5

Gene

UBP5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 2 (01 Jun 2002)
      Previous versions | rss
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    Functioni

    Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.1 Publication

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei326 – 3261Nucleophile
    Active sitei874 – 8741Proton acceptorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ubiquitin-specific protease activity Source: InterPro

    GO - Biological processi

    1. ubiquitin-dependent protein catabolic process Source: TAIR

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Ubl conjugation pathway

    Enzyme and pathway databases

    BioCyciARA:AT2G40930-MONOMER.

    Protein family/group databases

    MEROPSiC19.093.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 5 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 5
    Short name:
    AtUBP5
    Ubiquitin thioesterase 5
    Ubiquitin-specific-processing protease 5
    Gene namesi
    Name:UBP5
    Ordered Locus Names:At2g40930
    ORF Names:T20B5.13
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G40930.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: TAIR

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi326 – 3261C → S: Loss of activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 924924Ubiquitin carboxyl-terminal hydrolase 5PRO_0000080696Add
    BLAST

    Proteomic databases

    PaxDbiO22207.
    PRIDEiO22207.

    Expressioni

    Gene expression databases

    GenevestigatoriO22207.

    Interactioni

    Protein-protein interaction databases

    BioGridi4028. 1 interaction.
    IntActiO22207. 1 interaction.
    MINTiMINT-8064604.
    STRINGi3702.AT2G40930.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliO22207.
    SMRiO22207. Positions 26-243, 315-525, 737-918.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini15 – 145131DUSPPROSITE-ProRule annotationAdd
    BLAST
    Domaini317 – 916600USPAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase C19 family.Curated
    Contains 1 DUSP domain.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Phylogenomic databases

    eggNOGiCOG5560.
    HOGENOMiHOG000264375.
    InParanoidiO22207.
    KOiK11835.
    OMAiSSLSEHC.
    PhylomeDBiO22207.

    Family and domain databases

    Gene3Di3.30.2230.10. 1 hit.
    InterProiIPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view]
    PfamiPF06337. DUSP. 1 hit.
    PF00443. UCH. 1 hit.
    [Graphical view]
    SMARTiSM00695. DUSP. 1 hit.
    [Graphical view]
    SUPFAMiSSF143791. SSF143791. 1 hit.
    PROSITEiPS51283. DUSP. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O22207-1 [UniParc]FASTAAdd to Basket

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    MAEVSMGSSS SSTDLSPEEE RVFIRDIAIA AEANSKEGDT FYLITQRWWQ    50
    EWIEYVNQDQ PCNTNDGSSL SEHCDSPGSS TLKKPSRIDN SDLIYDSSLE 100
    DPSNTSEIIE TLQEGRDYVL LPQEVWNQLR SWYGGGPTLA RRVISSGLSQ 150
    TELAVEVYPL RLQLLLMPKS DHSAIRISKK ETIRELHRRA CEIFDLDSEH 200
    VRIWDYYGHQ KYSLMNDLDK TLDDANLQMD QDILVEVLDI NGTLSSAHIQ 250
    SAQENGLVDG DSTSILIEPS KSSLAAAGGF SSSRNAFRTG SVEVSQSFDN 300
    TYSSTGVTTR GSTAGLTGLL NLGNTCFMNS AIQCLVHTPE FASYFQEDYH 350
    QEINWQNPLG MVGELALAFG DLLRKLWAPG RTPIAPRPFK AKLARFAPQF 400
    SGYNQHDSQE LLAFLLDGLH EDLNRVKHKP YINSRDADGR PDEEVADEFW 450
    KNHIARNDSI IVDVCQGQYK STLVCPICNK VSVTFDPFMY LSLPLQFNTT 500
    RAITVTVFSC DKTALPSTIT VNVSKQGRCR DLIQALTNAC SLKQSEELKL 550
    AEIRNNFIHR LFEDPLIPLS SIKDDDHLAA YKLSKSSENT TLLRLVLRRR 600
    DQKAGEREST VQLKPCGTPL LSSASCGDAL TKGKIHCLVQ NMLSPFRREE 650
    SVGKKGNSDS SIPERRSARF NNTEEEDKVG GLKKAKKSNS SDLGASKLSL 700
    QLIDEDNKTI NLPDNEAEAM KLPSSATVTI YLDWTPELSG MYDITCLESL 750
    PEVLKYGPTT KKARSEPLSL YACLEAFLRE EPLVPDEMWF CPQCNERRQA 800
    SKKLDLWRLP EVLVIHLKRF SYSRSMKHKL ETFVNFPIHD LDLTKYVANK 850
    NLSQPQLYEL YALTNHYGGM GSGHYTAHIK LLDDSRWYNF DDSHISHINE 900
    DDVKSGAAYV LFYRRKSDAG GKMT 924
    Length:924
    Mass (Da):103,875
    Last modified:June 1, 2002 - v2
    Checksum:i0DF2EA93C8D2D51A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF048705 mRNA. Translation: AAF21246.1.
    AC002409 Genomic DNA. Translation: AAB86453.2.
    CP002685 Genomic DNA. Translation: AEC09900.1.
    AY099701 mRNA. Translation: AAM20552.1.
    BT000300 mRNA. Translation: AAN15619.1.
    PIRiT00757.
    RefSeqiNP_565944.1. NM_129656.3.
    UniGeneiAt.12368.

    Genome annotation databases

    EnsemblPlantsiAT2G40930.1; AT2G40930.1; AT2G40930.
    GeneIDi818691.
    KEGGiath:AT2G40930.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF048705 mRNA. Translation: AAF21246.1 .
    AC002409 Genomic DNA. Translation: AAB86453.2 .
    CP002685 Genomic DNA. Translation: AEC09900.1 .
    AY099701 mRNA. Translation: AAM20552.1 .
    BT000300 mRNA. Translation: AAN15619.1 .
    PIRi T00757.
    RefSeqi NP_565944.1. NM_129656.3.
    UniGenei At.12368.

    3D structure databases

    ProteinModelPortali O22207.
    SMRi O22207. Positions 26-243, 315-525, 737-918.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 4028. 1 interaction.
    IntActi O22207. 1 interaction.
    MINTi MINT-8064604.
    STRINGi 3702.AT2G40930.1-P.

    Protein family/group databases

    MEROPSi C19.093.

    Proteomic databases

    PaxDbi O22207.
    PRIDEi O22207.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G40930.1 ; AT2G40930.1 ; AT2G40930 .
    GeneIDi 818691.
    KEGGi ath:AT2G40930.

    Organism-specific databases

    GeneFarmi 4913.
    TAIRi AT2G40930.

    Phylogenomic databases

    eggNOGi COG5560.
    HOGENOMi HOG000264375.
    InParanoidi O22207.
    KOi K11835.
    OMAi SSLSEHC.
    PhylomeDBi O22207.

    Enzyme and pathway databases

    BioCyci ARA:AT2G40930-MONOMER.

    Gene expression databases

    Genevestigatori O22207.

    Family and domain databases

    Gene3Di 3.30.2230.10. 1 hit.
    InterProi IPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    [Graphical view ]
    Pfami PF06337. DUSP. 1 hit.
    PF00443. UCH. 1 hit.
    [Graphical view ]
    SMARTi SM00695. DUSP. 1 hit.
    [Graphical view ]
    SUPFAMi SSF143791. SSF143791. 1 hit.
    PROSITEi PS51283. DUSP. 1 hit.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Ubiquitin-specific proteases from Arabidopsis thaliana: cloning of AtUBP5 and analysis of substrate specificity of AtUBP3, AtUBP4, and AtUBP5 using Escherichia coli in vivo and in vitro assays."
      Rao-Naik C., Chandler J.S., McArdle B., Callis J.
      Arch. Biochem. Biophys. 379:198-208(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF CYS-326.
      Strain: cv. Landsberg erecta.
      Tissue: Flower.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    5. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
      Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
      Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.

    Entry informationi

    Entry nameiUBP5_ARATH
    AccessioniPrimary (citable) accession number: O22207
    Secondary accession number(s): Q9SEX3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 26, 2005
    Last sequence update: June 1, 2002
    Last modified: October 1, 2014
    This is version 101 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3