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Protein

Ubiquitin carboxyl-terminal hydrolase 5

Gene

UBP5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei326Nucleophile1
Active sitei874Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • protein deubiquitination Source: InterPro
  • ubiquitin-dependent protein catabolic process Source: TAIR

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

ReactomeiR-ATH-5689880 Ub-specific processing proteases

Protein family/group databases

MEROPSiC19.093

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 5 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 5
Short name:
AtUBP5
Ubiquitin thioesterase 5
Ubiquitin-specific-processing protease 5
Gene namesi
Name:UBP5
Ordered Locus Names:At2g40930
ORF Names:T20B5.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G40930
TAIRilocus:2058490 AT2G40930

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi326C → S: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000806961 – 924Ubiquitin carboxyl-terminal hydrolase 5Add BLAST924

Proteomic databases

PaxDbiO22207
PRIDEiO22207

PTM databases

iPTMnetiO22207

Expressioni

Gene expression databases

ExpressionAtlasiO22207 baseline and differential
GenevisibleiO22207 AT

Interactioni

Protein-protein interaction databases

BioGridi4028, 2 interactors
IntActiO22207, 1 interactor
STRINGi3702.AT2G40930.1

Structurei

3D structure databases

ProteinModelPortaliO22207
SMRiO22207
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 145DUSPPROSITE-ProRule annotationAdd BLAST131
Domaini317 – 916USPAdd BLAST600

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated

Phylogenomic databases

eggNOGiKOG1870 Eukaryota
COG5560 LUCA
HOGENOMiHOG000264375
InParanoidiO22207
OMAiAAVIFVN
OrthoDBiEOG09360187
PhylomeDBiO22207

Family and domain databases

InterProiView protein in InterPro
IPR035927 DUSP-like_sf
IPR006615 Pept_C19_DUSP
IPR001394 Peptidase_C19_UCH
IPR028134 USP4
IPR018200 USP_CS
IPR028889 USP_dom
PANTHERiPTHR43913 PTHR43913, 1 hit
PfamiView protein in Pfam
PF06337 DUSP, 1 hit
PF00443 UCH, 1 hit
SMARTiView protein in SMART
SM00695 DUSP, 1 hit
SUPFAMiSSF143791 SSF143791, 1 hit
PROSITEiView protein in PROSITE
PS51283 DUSP, 1 hit
PS00972 USP_1, 1 hit
PS00973 USP_2, 1 hit
PS50235 USP_3, 1 hit

Sequencei

Sequence statusi: Complete.

O22207-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEVSMGSSS SSTDLSPEEE RVFIRDIAIA AEANSKEGDT FYLITQRWWQ
60 70 80 90 100
EWIEYVNQDQ PCNTNDGSSL SEHCDSPGSS TLKKPSRIDN SDLIYDSSLE
110 120 130 140 150
DPSNTSEIIE TLQEGRDYVL LPQEVWNQLR SWYGGGPTLA RRVISSGLSQ
160 170 180 190 200
TELAVEVYPL RLQLLLMPKS DHSAIRISKK ETIRELHRRA CEIFDLDSEH
210 220 230 240 250
VRIWDYYGHQ KYSLMNDLDK TLDDANLQMD QDILVEVLDI NGTLSSAHIQ
260 270 280 290 300
SAQENGLVDG DSTSILIEPS KSSLAAAGGF SSSRNAFRTG SVEVSQSFDN
310 320 330 340 350
TYSSTGVTTR GSTAGLTGLL NLGNTCFMNS AIQCLVHTPE FASYFQEDYH
360 370 380 390 400
QEINWQNPLG MVGELALAFG DLLRKLWAPG RTPIAPRPFK AKLARFAPQF
410 420 430 440 450
SGYNQHDSQE LLAFLLDGLH EDLNRVKHKP YINSRDADGR PDEEVADEFW
460 470 480 490 500
KNHIARNDSI IVDVCQGQYK STLVCPICNK VSVTFDPFMY LSLPLQFNTT
510 520 530 540 550
RAITVTVFSC DKTALPSTIT VNVSKQGRCR DLIQALTNAC SLKQSEELKL
560 570 580 590 600
AEIRNNFIHR LFEDPLIPLS SIKDDDHLAA YKLSKSSENT TLLRLVLRRR
610 620 630 640 650
DQKAGEREST VQLKPCGTPL LSSASCGDAL TKGKIHCLVQ NMLSPFRREE
660 670 680 690 700
SVGKKGNSDS SIPERRSARF NNTEEEDKVG GLKKAKKSNS SDLGASKLSL
710 720 730 740 750
QLIDEDNKTI NLPDNEAEAM KLPSSATVTI YLDWTPELSG MYDITCLESL
760 770 780 790 800
PEVLKYGPTT KKARSEPLSL YACLEAFLRE EPLVPDEMWF CPQCNERRQA
810 820 830 840 850
SKKLDLWRLP EVLVIHLKRF SYSRSMKHKL ETFVNFPIHD LDLTKYVANK
860 870 880 890 900
NLSQPQLYEL YALTNHYGGM GSGHYTAHIK LLDDSRWYNF DDSHISHINE
910 920
DDVKSGAAYV LFYRRKSDAG GKMT
Length:924
Mass (Da):103,875
Last modified:June 1, 2002 - v2
Checksum:i0DF2EA93C8D2D51A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF048705 mRNA Translation: AAF21246.1
AC002409 Genomic DNA Translation: AAB86453.2
CP002685 Genomic DNA Translation: AEC09900.1
CP002685 Genomic DNA Translation: ANM61508.1
AY099701 mRNA Translation: AAM20552.1
BT000300 mRNA Translation: AAN15619.1
PIRiT00757
RefSeqiNP_001323724.1, NM_001336876.1
NP_565944.1, NM_129656.4
UniGeneiAt.12368

Genome annotation databases

EnsemblPlantsiAT2G40930.1; AT2G40930.1; AT2G40930
AT2G40930.2; AT2G40930.2; AT2G40930
GeneIDi818691
GrameneiAT2G40930.1; AT2G40930.1; AT2G40930
AT2G40930.2; AT2G40930.2; AT2G40930
KEGGiath:AT2G40930

Similar proteinsi

Entry informationi

Entry nameiUBP5_ARATH
AccessioniPrimary (citable) accession number: O22207
Secondary accession number(s): Q9SEX3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2002
Last modified: April 25, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health