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O22207

- UBP5_ARATH

UniProt

O22207 - UBP5_ARATH

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Protein

Ubiquitin carboxyl-terminal hydrolase 5

Gene
UBP5, At2g40930, T20B5.13
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei326 – 3261Nucleophile
Active sitei874 – 8741Proton acceptor By similarity

GO - Molecular functioni

  1. ubiquitin-specific protease activity Source: InterPro

GO - Biological processi

  1. ubiquitin-dependent protein catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciARA:AT2G40930-MONOMER.

Protein family/group databases

MEROPSiC19.093.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 5 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 5
Short name:
AtUBP5
Ubiquitin thioesterase 5
Ubiquitin-specific-processing protease 5
Gene namesi
Name:UBP5
Ordered Locus Names:At2g40930
ORF Names:T20B5.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G40930.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi326 – 3261C → S: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 924924Ubiquitin carboxyl-terminal hydrolase 5PRO_0000080696Add
BLAST

Proteomic databases

PaxDbiO22207.
PRIDEiO22207.

Expressioni

Gene expression databases

GenevestigatoriO22207.

Interactioni

Protein-protein interaction databases

BioGridi4028. 1 interaction.
IntActiO22207. 1 interaction.
MINTiMINT-8064604.
STRINGi3702.AT2G40930.1-P.

Structurei

3D structure databases

ProteinModelPortaliO22207.
SMRiO22207. Positions 26-243, 315-525, 737-918.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini15 – 145131DUSPAdd
BLAST
Domaini317 – 916600USPAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C19 family.
Contains 1 DUSP domain.
Contains 1 USP domain.

Phylogenomic databases

eggNOGiCOG5560.
HOGENOMiHOG000264375.
InParanoidiO22207.
KOiK11835.
OMAiSSLSEHC.
PhylomeDBiO22207.

Family and domain databases

Gene3Di3.30.2230.10. 1 hit.
InterProiIPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view]
PfamiPF06337. DUSP. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00695. DUSP. 1 hit.
[Graphical view]
SUPFAMiSSF143791. SSF143791. 1 hit.
PROSITEiPS51283. DUSP. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O22207-1 [UniParc]FASTAAdd to Basket

« Hide

MAEVSMGSSS SSTDLSPEEE RVFIRDIAIA AEANSKEGDT FYLITQRWWQ    50
EWIEYVNQDQ PCNTNDGSSL SEHCDSPGSS TLKKPSRIDN SDLIYDSSLE 100
DPSNTSEIIE TLQEGRDYVL LPQEVWNQLR SWYGGGPTLA RRVISSGLSQ 150
TELAVEVYPL RLQLLLMPKS DHSAIRISKK ETIRELHRRA CEIFDLDSEH 200
VRIWDYYGHQ KYSLMNDLDK TLDDANLQMD QDILVEVLDI NGTLSSAHIQ 250
SAQENGLVDG DSTSILIEPS KSSLAAAGGF SSSRNAFRTG SVEVSQSFDN 300
TYSSTGVTTR GSTAGLTGLL NLGNTCFMNS AIQCLVHTPE FASYFQEDYH 350
QEINWQNPLG MVGELALAFG DLLRKLWAPG RTPIAPRPFK AKLARFAPQF 400
SGYNQHDSQE LLAFLLDGLH EDLNRVKHKP YINSRDADGR PDEEVADEFW 450
KNHIARNDSI IVDVCQGQYK STLVCPICNK VSVTFDPFMY LSLPLQFNTT 500
RAITVTVFSC DKTALPSTIT VNVSKQGRCR DLIQALTNAC SLKQSEELKL 550
AEIRNNFIHR LFEDPLIPLS SIKDDDHLAA YKLSKSSENT TLLRLVLRRR 600
DQKAGEREST VQLKPCGTPL LSSASCGDAL TKGKIHCLVQ NMLSPFRREE 650
SVGKKGNSDS SIPERRSARF NNTEEEDKVG GLKKAKKSNS SDLGASKLSL 700
QLIDEDNKTI NLPDNEAEAM KLPSSATVTI YLDWTPELSG MYDITCLESL 750
PEVLKYGPTT KKARSEPLSL YACLEAFLRE EPLVPDEMWF CPQCNERRQA 800
SKKLDLWRLP EVLVIHLKRF SYSRSMKHKL ETFVNFPIHD LDLTKYVANK 850
NLSQPQLYEL YALTNHYGGM GSGHYTAHIK LLDDSRWYNF DDSHISHINE 900
DDVKSGAAYV LFYRRKSDAG GKMT 924
Length:924
Mass (Da):103,875
Last modified:June 1, 2002 - v2
Checksum:i0DF2EA93C8D2D51A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF048705 mRNA. Translation: AAF21246.1.
AC002409 Genomic DNA. Translation: AAB86453.2.
CP002685 Genomic DNA. Translation: AEC09900.1.
AY099701 mRNA. Translation: AAM20552.1.
BT000300 mRNA. Translation: AAN15619.1.
PIRiT00757.
RefSeqiNP_565944.1. NM_129656.3.
UniGeneiAt.12368.

Genome annotation databases

EnsemblPlantsiAT2G40930.1; AT2G40930.1; AT2G40930.
GeneIDi818691.
KEGGiath:AT2G40930.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF048705 mRNA. Translation: AAF21246.1 .
AC002409 Genomic DNA. Translation: AAB86453.2 .
CP002685 Genomic DNA. Translation: AEC09900.1 .
AY099701 mRNA. Translation: AAM20552.1 .
BT000300 mRNA. Translation: AAN15619.1 .
PIRi T00757.
RefSeqi NP_565944.1. NM_129656.3.
UniGenei At.12368.

3D structure databases

ProteinModelPortali O22207.
SMRi O22207. Positions 26-243, 315-525, 737-918.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 4028. 1 interaction.
IntActi O22207. 1 interaction.
MINTi MINT-8064604.
STRINGi 3702.AT2G40930.1-P.

Protein family/group databases

MEROPSi C19.093.

Proteomic databases

PaxDbi O22207.
PRIDEi O22207.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G40930.1 ; AT2G40930.1 ; AT2G40930 .
GeneIDi 818691.
KEGGi ath:AT2G40930.

Organism-specific databases

GeneFarmi 4913.
TAIRi AT2G40930.

Phylogenomic databases

eggNOGi COG5560.
HOGENOMi HOG000264375.
InParanoidi O22207.
KOi K11835.
OMAi SSLSEHC.
PhylomeDBi O22207.

Enzyme and pathway databases

BioCyci ARA:AT2G40930-MONOMER.

Gene expression databases

Genevestigatori O22207.

Family and domain databases

Gene3Di 3.30.2230.10. 1 hit.
InterProi IPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
[Graphical view ]
Pfami PF06337. DUSP. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view ]
SMARTi SM00695. DUSP. 1 hit.
[Graphical view ]
SUPFAMi SSF143791. SSF143791. 1 hit.
PROSITEi PS51283. DUSP. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Ubiquitin-specific proteases from Arabidopsis thaliana: cloning of AtUBP5 and analysis of substrate specificity of AtUBP3, AtUBP4, and AtUBP5 using Escherichia coli in vivo and in vitro assays."
    Rao-Naik C., Chandler J.S., McArdle B., Callis J.
    Arch. Biochem. Biophys. 379:198-208(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF CYS-326.
    Strain: cv. Landsberg erecta.
    Tissue: Flower.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine."
    Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D.
    Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.

Entry informationi

Entry nameiUBP5_ARATH
AccessioniPrimary (citable) accession number: O22207
Secondary accession number(s): Q9SEX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2002
Last modified: June 11, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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