O22207 (UBP5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 5
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 5
Short name=AtUBP5
Ubiquitin thioesterase 5
Ubiquitin-specific-processing protease 5

Gene names

Name:	UBP5
Ordered Locus Names:	At2g40930
ORF Names:	T20B5.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	924 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins. Ref.1
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Belongs to the peptidase C19 family. Contains 1 DUSP domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ubiquitin-dependent protein catabolic process Traceable author statement Ref.1. Source: TAIR
Cellular_component	nucleus Inferred from sequence or structural similarity Ref.1. Source: TAIR
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin thiolesterase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 924	924	Ubiquitin carboxyl-terminal hydrolase 5		PRO_0000080696
Regions
	Domain	15 – 145	131	DUSP
Sites
	Active site	326	1	Nucleophile
	Active site	874	1	Proton acceptor By similarity
Experimental info
	Mutagenesis	326	1	C → S: Loss of activity. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O22207 [UniParc]. Last modified June 1, 2002. Version 2. Checksum: 0DF2EA93C8D2D51A	FASTA	924	103,875
	10 20 30 40 50 60 MAEVSMGSSS SSTDLSPEEE RVFIRDIAIA AEANSKEGDT FYLITQRWWQ EWIEYVNQDQ 70 80 90 100 110 120 PCNTNDGSSL SEHCDSPGSS TLKKPSRIDN SDLIYDSSLE DPSNTSEIIE TLQEGRDYVL 130 140 150 160 170 180 LPQEVWNQLR SWYGGGPTLA RRVISSGLSQ TELAVEVYPL RLQLLLMPKS DHSAIRISKK 190 200 210 220 230 240 ETIRELHRRA CEIFDLDSEH VRIWDYYGHQ KYSLMNDLDK TLDDANLQMD QDILVEVLDI 250 260 270 280 290 300 NGTLSSAHIQ SAQENGLVDG DSTSILIEPS KSSLAAAGGF SSSRNAFRTG SVEVSQSFDN 310 320 330 340 350 360 TYSSTGVTTR GSTAGLTGLL NLGNTCFMNS AIQCLVHTPE FASYFQEDYH QEINWQNPLG 370 380 390 400 410 420 MVGELALAFG DLLRKLWAPG RTPIAPRPFK AKLARFAPQF SGYNQHDSQE LLAFLLDGLH 430 440 450 460 470 480 EDLNRVKHKP YINSRDADGR PDEEVADEFW KNHIARNDSI IVDVCQGQYK STLVCPICNK 490 500 510 520 530 540 VSVTFDPFMY LSLPLQFNTT RAITVTVFSC DKTALPSTIT VNVSKQGRCR DLIQALTNAC 550 560 570 580 590 600 SLKQSEELKL AEIRNNFIHR LFEDPLIPLS SIKDDDHLAA YKLSKSSENT TLLRLVLRRR 610 620 630 640 650 660 DQKAGEREST VQLKPCGTPL LSSASCGDAL TKGKIHCLVQ NMLSPFRREE SVGKKGNSDS 670 680 690 700 710 720 SIPERRSARF NNTEEEDKVG GLKKAKKSNS SDLGASKLSL QLIDEDNKTI NLPDNEAEAM 730 740 750 760 770 780 KLPSSATVTI YLDWTPELSG MYDITCLESL PEVLKYGPTT KKARSEPLSL YACLEAFLRE 790 800 810 820 830 840 EPLVPDEMWF CPQCNERRQA SKKLDLWRLP EVLVIHLKRF SYSRSMKHKL ETFVNFPIHD 850 860 870 880 890 900 LDLTKYVANK NLSQPQLYEL YALTNHYGGM GSGHYTAHIK LLDDSRWYNF DDSHISHINE 910 920 DDVKSGAAYV LFYRRKSDAG GKMT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Ubiquitin-specific proteases from Arabidopsis thaliana: cloning of AtUBP5 and analysis of substrate specificity of AtUBP3, AtUBP4, and AtUBP5 using Escherichia coli in vivo and in vitro assays." Rao-Naik C., Chandler J.S., McArdle B., Callis J. Arch. Biochem. Biophys. 379:198-208(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF CYS-326. Strain: cv. Landsberg erecta. Tissue: Flower.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"The ubiquitin-specific protease family from Arabidopsis. AtUBP1 and 2 are required for the resistance to the amino acid analog canavanine." Yan N., Doelling J.H., Falbel T.G., Durski A.M., Vierstra R.D. Plant Physiol. 124:1828-1843(2000) [PubMed] [Europe PMC] [Abstract] Cited for: GENE FAMILY ORGANIZATION, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF048705 mRNA. Translation: AAF21246.1. AC002409 Genomic DNA. Translation: AAB86453.2. CP002685 Genomic DNA. Translation: AEC09900.1. AY099701 mRNA. Translation: AAM20552.1. BT000300 mRNA. Translation: AAN15619.1.
IPI	IPI00536303.
PIR	T00757.
RefSeq	NP_565944.1. NM_129656.3.
UniGene	At.12368.
3D structure databases
ProteinModelPortal	O22207.
SMR	O22207. Positions 26-243, 315-525, 737-918.
ModBase	Search...
Protein-protein interaction databases
IntAct	O22207. 1 interaction.
STRING	3702.AT2G40930.1-P.
Protein family/group databases
MEROPS	C19.093.
Proteomic databases
PaxDb	O22207.
PRIDE	O22207.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G40930.1; AT2G40930.1; AT2G40930.
GeneID	818691.
KEGG	ath:AT2G40930.
Organism-specific databases
GeneFarm	4913.
TAIR	At2g40930.
Phylogenomic databases
eggNOG	COG5560.
HOGENOM	HOG000264375.
InParanoid	O22207.
KO	K11835.
OMA	ADEYWAN.
PhylomeDB	O22207.
ProtClustDB	CLSN2688853.
Gene expression databases
Genevestigator	O22207.
GermOnline	AT2G40930. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR006615. Pept_C19_DUSP. IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF06337. DUSP. 1 hit. PF00443. UCH. 1 hit. [Graphical view]
SMART	SM00695. DUSP. 1 hit. [Graphical view]
PROSITE	PS51283. DUSP. 1 hit. PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBP5_ARATH

Accession

Primary (citable) accession number: O22207
Secondary accession number(s): Q9SEX3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	June 1, 2002
Last modified:	May 1, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program