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Protein

Ubiquitin carboxyl-terminal hydrolase 5

Gene

UBP5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei326Nucleophile1
Active sitei874Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

  • protein deubiquitination Source: InterPro
  • ubiquitin-dependent protein catabolic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Enzyme and pathway databases

BioCyciARA:AT2G40930-MONOMER.

Protein family/group databases

MEROPSiC19.093.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 5 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 5
Short name:
AtUBP5
Ubiquitin thioesterase 5
Ubiquitin-specific-processing protease 5
Gene namesi
Name:UBP5
Ordered Locus Names:At2g40930
ORF Names:T20B5.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G40930.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: TAIR
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi326C → S: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000806961 – 924Ubiquitin carboxyl-terminal hydrolase 5Add BLAST924

Proteomic databases

PaxDbiO22207.
PRIDEiO22207.

PTM databases

iPTMnetiO22207.

Expressioni

Gene expression databases

GenevisibleiO22207. AT.

Interactioni

Protein-protein interaction databases

BioGridi4028. 2 interactors.
IntActiO22207. 1 interactor.
MINTiMINT-8064604.
STRINGi3702.AT2G40930.1.

Structurei

3D structure databases

ProteinModelPortaliO22207.
SMRiO22207.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini15 – 145DUSPPROSITE-ProRule annotationAdd BLAST131
Domaini317 – 916USPAdd BLAST600

Sequence similaritiesi

Belongs to the peptidase C19 family.Curated
Contains 1 DUSP domain.PROSITE-ProRule annotation
Contains 1 USP domain.Curated

Phylogenomic databases

eggNOGiKOG1870. Eukaryota.
COG5560. LUCA.
HOGENOMiHOG000264375.
InParanoidiO22207.
KOiK11835.
OMAiHQEINWQ.
OrthoDBiEOG09360187.
PhylomeDBiO22207.

Family and domain databases

Gene3Di3.30.2230.10. 1 hit.
InterProiIPR006615. Pept_C19_DUSP.
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF06337. DUSP. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00695. DUSP. 1 hit.
[Graphical view]
SUPFAMiSSF143791. SSF143791. 1 hit.
PROSITEiPS51283. DUSP. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O22207-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEVSMGSSS SSTDLSPEEE RVFIRDIAIA AEANSKEGDT FYLITQRWWQ
60 70 80 90 100
EWIEYVNQDQ PCNTNDGSSL SEHCDSPGSS TLKKPSRIDN SDLIYDSSLE
110 120 130 140 150
DPSNTSEIIE TLQEGRDYVL LPQEVWNQLR SWYGGGPTLA RRVISSGLSQ
160 170 180 190 200
TELAVEVYPL RLQLLLMPKS DHSAIRISKK ETIRELHRRA CEIFDLDSEH
210 220 230 240 250
VRIWDYYGHQ KYSLMNDLDK TLDDANLQMD QDILVEVLDI NGTLSSAHIQ
260 270 280 290 300
SAQENGLVDG DSTSILIEPS KSSLAAAGGF SSSRNAFRTG SVEVSQSFDN
310 320 330 340 350
TYSSTGVTTR GSTAGLTGLL NLGNTCFMNS AIQCLVHTPE FASYFQEDYH
360 370 380 390 400
QEINWQNPLG MVGELALAFG DLLRKLWAPG RTPIAPRPFK AKLARFAPQF
410 420 430 440 450
SGYNQHDSQE LLAFLLDGLH EDLNRVKHKP YINSRDADGR PDEEVADEFW
460 470 480 490 500
KNHIARNDSI IVDVCQGQYK STLVCPICNK VSVTFDPFMY LSLPLQFNTT
510 520 530 540 550
RAITVTVFSC DKTALPSTIT VNVSKQGRCR DLIQALTNAC SLKQSEELKL
560 570 580 590 600
AEIRNNFIHR LFEDPLIPLS SIKDDDHLAA YKLSKSSENT TLLRLVLRRR
610 620 630 640 650
DQKAGEREST VQLKPCGTPL LSSASCGDAL TKGKIHCLVQ NMLSPFRREE
660 670 680 690 700
SVGKKGNSDS SIPERRSARF NNTEEEDKVG GLKKAKKSNS SDLGASKLSL
710 720 730 740 750
QLIDEDNKTI NLPDNEAEAM KLPSSATVTI YLDWTPELSG MYDITCLESL
760 770 780 790 800
PEVLKYGPTT KKARSEPLSL YACLEAFLRE EPLVPDEMWF CPQCNERRQA
810 820 830 840 850
SKKLDLWRLP EVLVIHLKRF SYSRSMKHKL ETFVNFPIHD LDLTKYVANK
860 870 880 890 900
NLSQPQLYEL YALTNHYGGM GSGHYTAHIK LLDDSRWYNF DDSHISHINE
910 920
DDVKSGAAYV LFYRRKSDAG GKMT
Length:924
Mass (Da):103,875
Last modified:June 1, 2002 - v2
Checksum:i0DF2EA93C8D2D51A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF048705 mRNA. Translation: AAF21246.1.
AC002409 Genomic DNA. Translation: AAB86453.2.
CP002685 Genomic DNA. Translation: AEC09900.1.
AY099701 mRNA. Translation: AAM20552.1.
BT000300 mRNA. Translation: AAN15619.1.
PIRiT00757.
RefSeqiNP_565944.1. NM_129656.4.
UniGeneiAt.12368.

Genome annotation databases

EnsemblPlantsiAT2G40930.1; AT2G40930.1; AT2G40930.
GeneIDi818691.
GrameneiAT2G40930.1; AT2G40930.1; AT2G40930.
KEGGiath:AT2G40930.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF048705 mRNA. Translation: AAF21246.1.
AC002409 Genomic DNA. Translation: AAB86453.2.
CP002685 Genomic DNA. Translation: AEC09900.1.
AY099701 mRNA. Translation: AAM20552.1.
BT000300 mRNA. Translation: AAN15619.1.
PIRiT00757.
RefSeqiNP_565944.1. NM_129656.4.
UniGeneiAt.12368.

3D structure databases

ProteinModelPortaliO22207.
SMRiO22207.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4028. 2 interactors.
IntActiO22207. 1 interactor.
MINTiMINT-8064604.
STRINGi3702.AT2G40930.1.

Protein family/group databases

MEROPSiC19.093.

PTM databases

iPTMnetiO22207.

Proteomic databases

PaxDbiO22207.
PRIDEiO22207.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G40930.1; AT2G40930.1; AT2G40930.
GeneIDi818691.
GrameneiAT2G40930.1; AT2G40930.1; AT2G40930.
KEGGiath:AT2G40930.

Organism-specific databases

TAIRiAT2G40930.

Phylogenomic databases

eggNOGiKOG1870. Eukaryota.
COG5560. LUCA.
HOGENOMiHOG000264375.
InParanoidiO22207.
KOiK11835.
OMAiHQEINWQ.
OrthoDBiEOG09360187.
PhylomeDBiO22207.

Enzyme and pathway databases

BioCyciARA:AT2G40930-MONOMER.

Miscellaneous databases

PROiO22207.

Gene expression databases

GenevisibleiO22207. AT.

Family and domain databases

Gene3Di3.30.2230.10. 1 hit.
InterProiIPR006615. Pept_C19_DUSP.
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF06337. DUSP. 1 hit.
PF00443. UCH. 1 hit.
[Graphical view]
SMARTiSM00695. DUSP. 1 hit.
[Graphical view]
SUPFAMiSSF143791. SSF143791. 1 hit.
PROSITEiPS51283. DUSP. 1 hit.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBP5_ARATH
AccessioniPrimary (citable) accession number: O22207
Secondary accession number(s): Q9SEX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2002
Last modified: November 30, 2016
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.