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Protein

Probable pectinesterase/pectinesterase inhibitor 17

Gene

PME17

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Acts in the modification of cell walls via demethylesterification of cell wall pectin.By similarity

Catalytic activityi

Pectin + n H2O = n methanol + pectate.

Pathwayi: pectin degradation

This protein is involved in step 1 of the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Probable pectinesterase/pectinesterase inhibitor 7 (PME7), Pectinesterase (AXX17_At1g11890), Pectinesterase (AXX17_At4g02940), Pectinesterase (AXX17_At5g52320), Probable pectinesterase/pectinesterase inhibitor 42 (PME42), Probable pectinesterase 15 (PME15), Putative pectinesterase 14 (PME14), Pectinesterase (At3g10720), Pectinesterase (AXX17_At2g33410), Probable pectinesterase 55 (PME55), Probable pectinesterase/pectinesterase inhibitor 23 (PME23), Pectinesterase (AXX17_At4g18720), Pectinesterase 4 (PME4), Putative pectinesterase/pectinesterase inhibitor 22 (PME22), Pectinesterase (AXX17_At1g48290), Pectinesterase (AXX17_At5g47900), Pectinesterase (At3g14310), Pectinesterase (AXX17_At4g38050), Pectinesterase (AXX17_At5g07100), Pectinesterase/pectinesterase inhibitor 18 (PME18), Pectinesterase (AXX17_At2g14510), Pectinesterase (AXX17_At3g43380), Pectinesterase (AXX17_At5g64270), Putative pectinesterase 10 (PME10), Probable pectinesterase/pectinesterase inhibitor 64 (PME64), Pectinesterase (F14I3.7), Probable pectinesterase 29 (PME29), Pectinesterase, Pectinesterase (AXX17_At4g38040), Probable pectinesterase 8 (PME8), Pectinesterase (AXX17_At5g27870), Pectinesterase (AXX17_At5g18820), Pectinesterase (AXX17_At2g45340), Pectinesterase 31 (PME31), Pectinesterase (AXX17_At3g41380), Pectinesterase (AXX17_At1g11650), Probable pectinesterase/pectinesterase inhibitor 25 (PME25), Probable pectinesterase/pectinesterase inhibitor 51 (PME51), Pectinesterase (AXX17_At3g36460), Pectinesterase (AXX17_At5g04430), Pectinesterase (At3g62170), Probable pectinesterase/pectinesterase inhibitor 20 (PME20), Pectinesterase (T27B3.30), Probable pectinesterase/pectinesterase inhibitor 60 (PME60), Pectinesterase QRT1 (QRT1), Probable pectinesterase/pectinesterase inhibitor 59 (PME59), Pectinesterase (AXX17_At3g53530), Pectinesterase (AXX17_At5g61190), Pectinesterase PPME1 (PPME1), Pectinesterase (AXX17_At3g56460), Pectinesterase (AXX17_At3g06710), Probable pectinesterase/pectinesterase inhibitor 32 (PME32), Pectinesterase (AXX17_At1g44540), Probable pectinesterase/pectinesterase inhibitor 33 (PME33), Probable pectinesterase/pectinesterase inhibitor 36 (PME36), Probable pectinesterase/pectinesterase inhibitor 13 (PME13), Putative pectinesterase 52 (PME52), Probable pectinesterase/pectinesterase inhibitor 54 (PME54), Pectinesterase (AXX17_At2g33420), Pectinesterase (AXX17_At2g40510), Pectinesterase (At1g53840), Pectinesterase 5 (PME5), Probable pectinesterase/pectinesterase inhibitor 16 (PME16), Probable pectinesterase 30 (PME30), Probable pectinesterase/pectinesterase inhibitor VGDH2 (VGDH2), Pectinesterase (AXX17_At3g26070), Probable pectinesterase 68 (PME68), Pectinesterase, Pectinesterase (AXX17_At3g14900), Pectinesterase (AXX17_At5g54740), Probable pectinesterase 67 (PME67), Putative pectinesterase/pectinesterase inhibitor 38 (PME38), Probable pectinesterase 56 (PME56), Pectinesterase, Putative pectinesterase/pectinesterase inhibitor 28 (PME28), Probable pectinesterase/pectinesterase inhibitor 47 (PME47), Probable pectinesterase/pectinesterase inhibitor 46 (PME46), Probable pectinesterase 53 (PME53), Pectinesterase (AXX17_At5g07090), Probable pectinesterase/pectinesterase inhibitor 17 (PME17), Pectinesterase (AXX17_At2g22270), Probable pectinesterase/pectinesterase inhibitor 61 (PME61), Probable pectinesterase 66 (PME66), Pectinesterase (AXX17_At2g42790), Pectinesterase (AXX17_At5g45990), Probable pectinesterase/pectinesterase inhibitor 6 (PME6), Probable pectinesterase 48 (PME48), Probable pectinesterase 49 (PME49), Pectinesterase (AXX17_At1g64100), Probable pectinesterase 50 (PME50), Pectinesterase (AXX17_At3g17940), Pectinesterase, Probable pectinesterase/pectinesterase inhibitor 19 (PME19), Pectinesterase (AXX17_At2g45050), Probable pectinesterase/pectinesterase inhibitor 40 (PME40), Pectinesterase (AXX17_At1g11920), Pectinesterase (AXX17_At3g30550), PME26 (AXX17_At3g14890), Probable pectinesterase/pectinesterase inhibitor 39 (PME39), Pectinesterase (AXX17_At2g44790), Pectinesterase (AXX17_At2g17140), Pectinesterase (AXX17_At5g19650), Putative pectinesterase/pectinesterase inhibitor 43 (PME43), Pectinesterase (AXX17_At3g31890), Pectinesterase (AXX17_At4g02900), Probable pectinesterase/pectinesterase inhibitor 34 (PME34), Pectinesterase 1 (PME1), Putative pectinesterase 63 (PME63), Pectinesterase (AXX17_At3g05180), Pectinesterase 2 (PME2), Probable pectinesterase/pectinesterase inhibitor 21 (PME21), Pectinesterase (AXX17_At2g22280), Pectinesterase (AXX17_At1g51200), Putative pectinesterase/pectinesterase inhibitor 45 (PME45), Probable pectinesterase/pectinesterase inhibitor 12 (PME12), Pectinesterase (AXX17_At4g02930), Probable pectinesterase/pectinesterase inhibitor 44 (PME44), Pectinesterase, Pectinesterase/pectinesterase inhibitor 3 (PME3), Pectinesterase (AXX17_At1g24390), Pectinesterase (AXX17_At5g50300), Pectinesterase (AXX17_At1g11900), Probable pectinesterase/pectinesterase inhibitor 58 (PME58), Putative pectinesterase 57 (PME57), Pectinesterase (AXX17_At3g10560), Pectinesterase (AXX17_At3g55110), Pectinesterase (AXX17_At3g10570), Putative pectinesterase 11 (PME11), Pectinesterase (AXX17_At1g01970), Pectinesterase (AXX17_At5g07110), Pectinesterase (AXX17_At4g06570), Pectinesterase (AXX17_At5g50310), Pectinesterase, Pectinesterase (AXX17_At1g51190), Pectinesterase (AXX17_At2g33420), Pectinesterase (AXX17_At1g48300), Pectinesterase (AXX17_At3g05190), Putative pectinesterase/pectinesterase inhibitor 24 (PME24), Putative pectinesterase/pectinesterase inhibitor 26 (PME26), Probable pectinesterase/pectinesterase inhibitor 35 (PME35), Pectinesterase (AXX17_At5g04420), Pectinesterase (AXX17_At2g44780), Pectinesterase (AXX17_At4g00230), Pectinesterase (AXX17_At5g09310), Pectinesterase (AXX17_At5g20800), Pectinesterase (AXX17_At1g04730), Probable pectinesterase/pectinesterase inhibitor 41 (PME41), Pectinesterase (AXX17_At1g11660), Pectinesterase (AXX17_At5g26780)
  2. Pectate lyase (AXX17_At3g26720), Probable pectate lyase 7 (At3g01270), Probable pectate lyase 18 (At4g24780), Probable pectate lyase 16 (At4g22080), Putative pectate lyase 17 (At4g22090), Pectate lyase (AXX17_At3g09180), Putative pectate lyase 14 (At4g13210), Pectate lyase (At3g55140), Pectate lyase (AXX17_At5g54880), Pectate lyase (AXX17_At4g15660), Probable pectate lyase 22 (At5g63180), Pectate lyase (T5E8_80), Pectate lyase (AXX17_At3g00310), Pectate lyase (At3g07010), Probable pectate lyase 8 (At3g07010), Probable pectate lyase 4 (At1g30350), Pectate lyase (AXX17_At2g01680), Putative pectate lyase 21 (At5g55720), Pectate lyase (At4g13210), Pectate lyase (At5g04310), Pectate lyase (AXX17_At5g03700), Pectate lyase (At4g13710), Pectate lyase (AXX17_At3g49720), Pectate lyase (At3g01270), Pectate lyase (At5g04310), Pectate lyase (AXX17_At1g12300), Probable pectate lyase 9 (At3g24230), Probable pectate lyase 1 (At1g04680), Pectate lyase (AXX17_At5g08800), Pectate lyase (T26I12.20), Pectate lyase (AXX17_At5g62760), Pectate lyase (AXX17_At3g47590), Pectate lyase (AXX17_At4g14900), Pectate lyase (AXX17_At3g49450), Pectate lyase (AXX17_At4g25600), Pectate lyase (AXX17_At1g61710), Probable pectate lyase 20 (At5g48900), Pectate lyase (AXX17_At5g47570), Probable pectate lyase 6 (At2g02720), Pectate lyase (AXX17_At3g06910), Pectate lyase (At1g14420), Pectate lyase (At3g01270), Pectate lyase (At2g02720), Pectate lyase (AXX17_At1g04020), Pectate lyase (At3g01270), Pectate lyase (At3g55140), Putative pectate lyase 2 (At1g11920), Pectate lyase, Pectate lyase (AXX17_At1g30780), Probable pectate lyase 19 (At5g15110), Pectate lyase (F11F8_12), Probable pectate lyase 13 (PMR6), Probable pectate lyase 3 (AT59), Pectate lyase (AXX17_At3g26200), Probable pectate lyase 5 (At1g67750), Probable pectate lyase 12 (At3g53190), Pectate lyase (At4g24780), Pectate lyase (AXX17_At4g25610), Probable pectate lyase 15 (At4g13710), Pectate lyase (At3g53190), Putative pectate lyase 11 (At3g27400), Pectate lyase (AXX17_At1g15010), Pectate lyase (At3g07010), Pectate lyase (AXX17_At3g29900), Pectate lyase (AXX17_At5g14600), Probable pectate lyase 10 (At3g24670)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway pectin degradation, which is itself part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 2-dehydro-3-deoxy-D-gluconate from pectin, the pathway pectin degradation and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei277Substrate; for pectinesterase activityBy similarity1
Binding sitei307Substrate; for pectinesterase activityBy similarity1
Sitei329Transition state stabilizerBy similarity1
Active sitei330Proton donor; for pectinesterase activityPROSITE-ProRule annotation1
Active sitei351Nucleophile; for pectinesterase activityPROSITE-ProRule annotation1
Binding sitei418Substrate; for pectinesterase activityBy similarity1
Binding sitei420Substrate; for pectinesterase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cell wall modification Source: InterPro
  • defense response to Gram-negative bacterium Source: TAIR
  • pectin catabolic process Source: UniProtKB-UniPathway
  • response to bacterium Source: TAIR
  • response to fungus Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Aspartyl esterase, Hydrolase

Keywords - Biological processi

Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT2G45220-MONOMER.
UniPathwayiUPA00545; UER00823.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable pectinesterase/pectinesterase inhibitor 17
Including the following 2 domains:
Pectinesterase inhibitor 17
Alternative name(s):
Pectin methylesterase inhibitor 17
Pectinesterase 17 (EC:3.1.1.11)
Short name:
PE 17
Alternative name(s):
Pectin methylesterase 17
Short name:
AtPME17
Gene namesi
Name:PME17
Synonyms:ARATH17
Ordered Locus Names:At2g45220
ORF Names:F4L23.27
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G45220.

Subcellular locationi

GO - Cellular componenti

  • cell periphery Source: GO_Central
  • cell wall Source: UniProtKB-SubCell
  • extracellular region Source: UniProtKB-KW
  • membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000037167424 – 511Probable pectinesterase/pectinesterase inhibitor 17Add BLAST488

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi112N-linked (GlcNAc...)Sequence analysis1
Glycosylationi160N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi344 ↔ 364By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO22149.
PRIDEiO22149.

Expressioni

Tissue specificityi

Expressed in siliques.2 Publications

Developmental stagei

Expressed during late developmental phases of siliques.1 Publication

Gene expression databases

GenevisibleiO22149. AT.

Interactioni

Protein-protein interaction databases

BioGridi4466. 2 interactors.
IntActiO22149. 2 interactors.
STRINGi3702.AT2G45220.1.

Structurei

3D structure databases

ProteinModelPortaliO22149.
SMRiO22149.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni24 – 171Pectinesterase inhibitor 17Add BLAST148
Regioni237 – 414Pectinesterase 17Add BLAST178

Sequence similaritiesi

In the N-terminal section; belongs to the PMEI family.Curated
In the C-terminal section; belongs to the pectinesterase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IF38. Eukaryota.
COG4677. LUCA.
HOGENOMiHOG000217409.
InParanoidiO22149.
KOiK01051.
OMAiFLTHNSN.
OrthoDBiEOG093607RJ.
PhylomeDBiO22149.

Family and domain databases

Gene3Di1.20.140.40. 1 hit.
2.160.20.10. 1 hit.
InterProiIPR006633. Carb-bd_sugar_hydrolysis-dom.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib_dom.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
SMARTiSM00722. CASH. 1 hit.
SM00856. PMEI. 1 hit.
[Graphical view]
SUPFAMiSSF101148. SSF101148. 1 hit.
SSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR01614. PME_inhib. 1 hit.
PROSITEiPS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O22149-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMAFRAYIIN FVILCILVAS TVSGYNQKDV KAWCSQTPNP KPCEYFLTHN
60 70 80 90 100
SNNEPIKSES EFLKISMKLV LDRAILAKTH AFTLGPKCRD TREKAAWEDC
110 120 130 140 150
IKLYDLTVSK INETMDPNVK CSKLDAQTWL STALTNLDTC RAGFLELGVT
160 170 180 190 200
DIVLPLMSNN VSNLLCNTLA INKVPFNYTP PEKDGFPSWV KPGDRKLLQS
210 220 230 240 250
STPKDNAVVA KDGSGNFKTI KEAIDAASGS GRFVIYVKQG VYSENLEIRK
260 270 280 290 300
KNVMLRGDGI GKTIITGSKS VGGGTTTFNS ATVAAVGDGF IARGITFRNT
310 320 330 340 350
AGASNEQAVA LRSGSDLSVF YQCSFEAYQD TLYVHSNRQF YRDCDVYGTV
360 370 380 390 400
DFIFGNAAAV LQNCNIFARR PRSKTNTITA QGRSDPNQNT GIIIHNSRVT
410 420 430 440 450
AASDLRPVLG STKTYLGRPW RQYSRTVFMK TSLDSLIDPR GWLEWDGNFA
460 470 480 490 500
LKTLFYAEFQ NTGPGASTSG RVTWPGFRVL GSASEASKFT VGTFLAGGSW
510
IPSSVPFTSG L
Length:511
Mass (Da):55,977
Last modified:June 1, 2002 - v2
Checksum:i96487096A87F9375
GO

Sequence cautioni

The sequence BAD93862 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002387 Genomic DNA. Translation: AAB82640.2.
CP002685 Genomic DNA. Translation: AEC10527.1.
AF361829 mRNA. Translation: AAK32841.1.
AK220726 mRNA. Translation: BAD93862.1. Different initiation.
PIRiH84887.
RefSeqiNP_566038.1. NM_130085.4.
UniGeneiAt.27946.

Genome annotation databases

EnsemblPlantsiAT2G45220.1; AT2G45220.1; AT2G45220.
GeneIDi819130.
GrameneiAT2G45220.1; AT2G45220.1; AT2G45220.
KEGGiath:AT2G45220.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC002387 Genomic DNA. Translation: AAB82640.2.
CP002685 Genomic DNA. Translation: AEC10527.1.
AF361829 mRNA. Translation: AAK32841.1.
AK220726 mRNA. Translation: BAD93862.1. Different initiation.
PIRiH84887.
RefSeqiNP_566038.1. NM_130085.4.
UniGeneiAt.27946.

3D structure databases

ProteinModelPortaliO22149.
SMRiO22149.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4466. 2 interactors.
IntActiO22149. 2 interactors.
STRINGi3702.AT2G45220.1.

Proteomic databases

PaxDbiO22149.
PRIDEiO22149.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G45220.1; AT2G45220.1; AT2G45220.
GeneIDi819130.
GrameneiAT2G45220.1; AT2G45220.1; AT2G45220.
KEGGiath:AT2G45220.

Organism-specific databases

TAIRiAT2G45220.

Phylogenomic databases

eggNOGiENOG410IF38. Eukaryota.
COG4677. LUCA.
HOGENOMiHOG000217409.
InParanoidiO22149.
KOiK01051.
OMAiFLTHNSN.
OrthoDBiEOG093607RJ.
PhylomeDBiO22149.

Enzyme and pathway databases

UniPathwayiUPA00545; UER00823.
BioCyciARA:AT2G45220-MONOMER.

Miscellaneous databases

PROiO22149.

Gene expression databases

GenevisibleiO22149. AT.

Family and domain databases

Gene3Di1.20.140.40. 1 hit.
2.160.20.10. 1 hit.
InterProiIPR006633. Carb-bd_sugar_hydrolysis-dom.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
IPR033131. Pectinesterase_Asp_AS.
IPR000070. Pectinesterase_cat.
IPR006501. Pectinesterase_inhib_dom.
[Graphical view]
PfamiPF01095. Pectinesterase. 1 hit.
PF04043. PMEI. 1 hit.
[Graphical view]
SMARTiSM00722. CASH. 1 hit.
SM00856. PMEI. 1 hit.
[Graphical view]
SUPFAMiSSF101148. SSF101148. 1 hit.
SSF51126. SSF51126. 1 hit.
TIGRFAMsiTIGR01614. PME_inhib. 1 hit.
PROSITEiPS00503. PECTINESTERASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPME17_ARATH
AccessioniPrimary (citable) accession number: O22149
Secondary accession number(s): Q570I2, Q9ASU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: June 1, 2002
Last modified: November 30, 2016
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The PMEI region may act as an autoinhibitory domain and prevent untimely PME activity during transport.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.