Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial

Gene

GCP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in mitochondrial tRNAs that read codons beginning with adenine. Probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Involved in mitochondrial genome maintenance (By similarity). May have a role in embryonic development in plants.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N6-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

a divalent metal cationUniRule annotationNote: Binds 1 divalent metal cation per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi194Divalent metal cationUniRule annotation1
Metal bindingi198Divalent metal cationUniRule annotation1
Binding sitei250SubstrateUniRule annotation1
Binding sitei265Substrate; via amide nitrogenUniRule annotation1
Binding sitei269SubstrateUniRule annotation1
Binding sitei401SubstrateUniRule annotation1
Metal bindingi402Divalent metal cationUniRule annotation1

GO - Molecular functioni

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • tRNA threonylcarbamoyladenosine modification Source: GO_Central

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processtRNA processing
LigandMetal-binding

Enzyme and pathway databases

BioCyciARA:AT2G45270-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrialUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
Glycoprotease 1UniRule annotation
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein GCP1UniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein GCP1UniRule annotation
Gene namesi
Name:GCP1UniRule annotation
Ordered Locus Names:At2g45270
ORF Names:F4L23.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G45270
TAIRilocus:2050872 AT2G45270

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Disruption phenotypei

Embryonic lethal.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 86MitochondrionUniRule annotationAdd BLAST86
ChainiPRO_000042453687 – 480Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrialAdd BLAST394

Proteomic databases

PaxDbiO22145

Expressioni

Tissue specificityi

Expressed in young developing leaves, roots, flowers and siliques.1 Publication

Developmental stagei

Expressed transiently at the early stages of seedling development. Maximal expression is reached during week 3 after seed germination.1 Publication

Gene expression databases

ExpressionAtlasiO22145 baseline and differential
GenevisibleiO22145 AT

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi3702.AT2G45270.1

Structurei

3D structure databases

ProteinModelPortaliO22145
SMRiO22145
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni217 – 221Substrate bindingUniRule annotation5
Regioni373 – 374Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2707 Eukaryota
COG0533 LUCA
HOGENOMiHOG000109570
KOiK01409
OMAiHLEGHIY
OrthoDBiEOG09360APH
PhylomeDBiO22145

Family and domain databases

HAMAPiMF_01445 TsaD, 1 hit
InterProiView protein in InterPro
IPR000905 Gcp-like_dom
IPR017861 KAE1/TsaD
IPR022450 TsaD
PANTHERiPTHR11735 PTHR11735, 1 hit
PTHR11735:SF6 PTHR11735:SF6, 1 hit
PfamiView protein in Pfam
PF00814 Peptidase_M22, 1 hit
PRINTSiPR00789 OSIALOPTASE
TIGRFAMsiTIGR00329 gcp_kae1, 1 hit
TIGR03723 T6A_TsaD_YgjD, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O22145-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRLFLTLSP AISRFNLYPG ISILARNNNS LRLQKHHKLK TKTPTFSLIS
60 70 80 90 100
PSSSPNFQRT RFYSTETRIS SLPYSENPNF DDNLVVLGIE TSCDDTAAAV
110 120 130 140 150
VRGNGEILSQ VISSQAELLV QYGGVAPKQA EEAHSRVIDK VVQDALDKAN
160 170 180 190 200
LTEKDLSAVA VTIGPGLSLC LRVGVRKARR VAGNFSLPIV GVHHMEAHAL
210 220 230 240 250
VARLVEQELS FPFMALLISG GHNLLVLAHK LGQYTQLGTT VDDAIGEAFD
260 270 280 290 300
KTAKWLGLDM HRSGGPAVEE LALEGDAKSV KFNVPMKYHK DCNFSYAGLK
310 320 330 340 350
TQVRLAIEAK EIDAKCPVSS ATNEDRRNRA DIAASFQRVA VLHLEEKCER
360 370 380 390 400
AIDWALELEP SIKHMVISGG VASNKYVRLR LNNIVENKNL KLVCPPPSLC
410 420 430 440 450
TDNGVMVAWT GLEHFRVGRY DPPPPATEPE DYVYDLRPRW PLGEEYAKGR
460 470 480
SEARSMRTAR IHPSLTSIIR ADSLQQQTQT
Length:480
Mass (Da):52,996
Last modified:June 1, 2002 - v2
Checksum:i20DD6A86ACC1FFAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY024338 mRNA Translation: AAK00530.1
AC002387 Genomic DNA Translation: AAB82636.2
CP002685 Genomic DNA Translation: AEC10532.1
AY063864 mRNA Translation: AAL36220.1
AY117283 mRNA Translation: AAM51358.1
PIRiE84888
RefSeqiNP_566039.1, NM_130090.5
UniGeneiAt.27172

Genome annotation databases

EnsemblPlantsiAT2G45270.1; AT2G45270.1; AT2G45270
GeneIDi819135
GrameneiAT2G45270.1; AT2G45270.1; AT2G45270
KEGGiath:AT2G45270

Similar proteinsi

Entry informationi

Entry nameiOSGP2_ARATH
AccessioniPrimary (citable) accession number: O22145
Secondary accession number(s): Q8VWL2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: June 1, 2002
Last modified: April 25, 2018
This is version 133 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health