Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrial

Gene

GCP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in mitochondrial tRNAs that read codons beginning with adenine. Probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Involved in mitochondrial genome maintenance (By similarity). May have a role in embryonic development in plants.UniRule annotation

Catalytic activityi

L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA.UniRule annotation

Cofactori

a divalent metal cationUniRule annotationNote: Binds 1 divalent metal cation per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi194 – 1941Divalent metal cationUniRule annotation
Metal bindingi198 – 1981Divalent metal cationUniRule annotation
Binding sitei250 – 2501SubstrateUniRule annotation
Binding sitei265 – 2651Substrate; via amide nitrogenUniRule annotation
Binding sitei269 – 2691SubstrateUniRule annotation
Binding sitei401 – 4011SubstrateUniRule annotation
Metal bindingi402 – 4021Divalent metal cationUniRule annotation

GO - Molecular functioni

GO - Biological processi

  • embryo development ending in seed dormancy Source: TAIR
  • tRNA threonylcarbamoyladenosine modification Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

Metal-binding

Enzyme and pathway databases

BioCyciARA:AT2G45270-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrialUniRule annotation (EC:2.3.1.234UniRule annotation)
Alternative name(s):
Glycoprotease 1UniRule annotation
N6-L-threonylcarbamoyladenine synthaseUniRule annotation
Short name:
t(6)A synthaseUniRule annotation
t(6)A37 threonylcarbamoyladenosine biosynthesis protein GCP1UniRule annotation
tRNA threonylcarbamoyladenosine biosynthesis protein GCP1UniRule annotation
Gene namesi
Name:GCP1UniRule annotation
Ordered Locus Names:At2g45270
ORF Names:F4L23.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G45270.

Subcellular locationi

GO - Cellular componenti

  • EKC/KEOPS complex Source: GO_Central
  • mitochondrial inner membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Disruption phenotypei

Embryonic lethal.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 8686MitochondrionUniRule annotationAdd
BLAST
Chaini87 – 480394Probable tRNA N6-adenosine threonylcarbamoyltransferase, mitochondrialPRO_0000424536Add
BLAST

Proteomic databases

PaxDbiO22145.
PRIDEiO22145.

Expressioni

Tissue specificityi

Expressed in young developing leaves, roots, flowers and siliques.1 Publication

Developmental stagei

Expressed transiently at the early stages of seedling development. Maximal expression is reached during week 3 after seed germination.1 Publication

Gene expression databases

GenevisibleiO22145. AT.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi3702.AT2G45270.1.

Structurei

3D structure databases

ProteinModelPortaliO22145.
SMRiO22145. Positions 86-444.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni217 – 2215Substrate bindingUniRule annotation
Regioni373 – 3742Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the KAE1 / TsaD family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2707. Eukaryota.
COG0533. LUCA.
HOGENOMiHOG000109570.
KOiK01409.
OMAiICIDHIL.
PhylomeDBiO22145.

Family and domain databases

HAMAPiMF_01445. TsaD.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_TsaD_YgjD. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O22145-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRLFLTLSP AISRFNLYPG ISILARNNNS LRLQKHHKLK TKTPTFSLIS
60 70 80 90 100
PSSSPNFQRT RFYSTETRIS SLPYSENPNF DDNLVVLGIE TSCDDTAAAV
110 120 130 140 150
VRGNGEILSQ VISSQAELLV QYGGVAPKQA EEAHSRVIDK VVQDALDKAN
160 170 180 190 200
LTEKDLSAVA VTIGPGLSLC LRVGVRKARR VAGNFSLPIV GVHHMEAHAL
210 220 230 240 250
VARLVEQELS FPFMALLISG GHNLLVLAHK LGQYTQLGTT VDDAIGEAFD
260 270 280 290 300
KTAKWLGLDM HRSGGPAVEE LALEGDAKSV KFNVPMKYHK DCNFSYAGLK
310 320 330 340 350
TQVRLAIEAK EIDAKCPVSS ATNEDRRNRA DIAASFQRVA VLHLEEKCER
360 370 380 390 400
AIDWALELEP SIKHMVISGG VASNKYVRLR LNNIVENKNL KLVCPPPSLC
410 420 430 440 450
TDNGVMVAWT GLEHFRVGRY DPPPPATEPE DYVYDLRPRW PLGEEYAKGR
460 470 480
SEARSMRTAR IHPSLTSIIR ADSLQQQTQT
Length:480
Mass (Da):52,996
Last modified:June 1, 2002 - v2
Checksum:i20DD6A86ACC1FFAD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY024338 mRNA. Translation: AAK00530.1.
AC002387 Genomic DNA. Translation: AAB82636.2.
CP002685 Genomic DNA. Translation: AEC10532.1.
AY063864 mRNA. Translation: AAL36220.1.
AY117283 mRNA. Translation: AAM51358.1.
PIRiE84888.
RefSeqiNP_566039.1. NM_130090.4.
UniGeneiAt.27172.

Genome annotation databases

EnsemblPlantsiAT2G45270.1; AT2G45270.1; AT2G45270.
GeneIDi819135.
GrameneiAT2G45270.1; AT2G45270.1; AT2G45270.
KEGGiath:AT2G45270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY024338 mRNA. Translation: AAK00530.1.
AC002387 Genomic DNA. Translation: AAB82636.2.
CP002685 Genomic DNA. Translation: AEC10532.1.
AY063864 mRNA. Translation: AAL36220.1.
AY117283 mRNA. Translation: AAM51358.1.
PIRiE84888.
RefSeqiNP_566039.1. NM_130090.4.
UniGeneiAt.27172.

3D structure databases

ProteinModelPortaliO22145.
SMRiO22145. Positions 86-444.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G45270.1.

Proteomic databases

PaxDbiO22145.
PRIDEiO22145.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G45270.1; AT2G45270.1; AT2G45270.
GeneIDi819135.
GrameneiAT2G45270.1; AT2G45270.1; AT2G45270.
KEGGiath:AT2G45270.

Organism-specific databases

TAIRiAT2G45270.

Phylogenomic databases

eggNOGiKOG2707. Eukaryota.
COG0533. LUCA.
HOGENOMiHOG000109570.
KOiK01409.
OMAiICIDHIL.
PhylomeDBiO22145.

Enzyme and pathway databases

BioCyciARA:AT2G45270-MONOMER.

Miscellaneous databases

PROiO22145.

Gene expression databases

GenevisibleiO22145. AT.

Family and domain databases

HAMAPiMF_01445. TsaD.
InterProiIPR000905. Gcp-like_dom.
IPR017861. KAE1/TsaD.
IPR022450. TsaD.
[Graphical view]
PfamiPF00814. Peptidase_M22. 1 hit.
[Graphical view]
PRINTSiPR00789. OSIALOPTASE.
TIGRFAMsiTIGR00329. gcp_kae1. 1 hit.
TIGR03723. T6A_TsaD_YgjD. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Eukaryotic GCP1 is a conserved mitochondrial protein required for progression of embryo development beyond the globular stage in Arabidopsis thaliana."
    Haussuehl K., Huesgen P.F., Meier M., Dessi P., Glaser E., Adamski J., Adamska I.
    Biochem. J. 423:333-341(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiOSGP2_ARATH
AccessioniPrimary (citable) accession number: O22145
Secondary accession number(s): Q8VWL2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2013
Last sequence update: June 1, 2002
Last modified: June 8, 2016
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.