Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

UDP-glucuronate 4-epimerase 4

Gene

GAE4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the synthesis of the negatively charged monosaccharide that forms the backbone of pectic cell wall components.

Catalytic activityi

UDP-glucuronate = UDP-D-galacturonate.

Enzyme regulationi

Activated by glycerol, not effected by dimethyl sulfoxide and inhibited by high concentration of monovalent salts, UDP-xylose, UDP-arabinose or UDP.1 Publication

Kineticsi

Equilibrium between UDP-glucuronate and UDP-D-galacturonate established at 1:1.9. No activity with UDP-Galactose, UDP-Glucose, UDP-arabinose, UDP-Xylose, CDP-glucose or GDP-manose.
  1. KM=0.72 mM for UDP-glucuronate1 Publication

    pH dependencei

    Optimum pH is 7.5. Active from pH 6.8 to 8.2.1 Publication

    Temperature dependencei

    Active between 20 and 55 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei248Proton acceptorBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi98 – 129NADBy similarityAdd BLAST32

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionIsomerase
    Biological processCarbohydrate metabolism
    LigandNAD

    Enzyme and pathway databases

    BioCyciARA:AT2G45310-MONOMER
    MetaCyc:AT2G45310-MONOMER
    BRENDAi5.1.3.6 399
    SABIO-RKO22141

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-glucuronate 4-epimerase 4 (EC:5.1.3.6)
    Alternative name(s):
    UDP-glucuronic acid epimerase 4
    Short name:
    AtUGlcAE1
    Gene namesi
    Name:GAE4
    Synonyms:UGlcAE1
    Ordered Locus Names:At2g45310
    ORF Names:F4L23
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    Proteomesi
    • UP000006548 Componenti: Chromosome 2

    Organism-specific databases

    AraportiAT2G45310
    TAIRilocus:2050921 AT2G45310

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei30 – 50HelicalSequence analysisAdd BLAST21
    Transmembranei96 – 116HelicalSequence analysisAdd BLAST21

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002925991 – 437UDP-glucuronate 4-epimerase 4Add BLAST437

    Proteomic databases

    PaxDbiO22141

    PTM databases

    iPTMnetiO22141

    Expressioni

    Tissue specificityi

    In roots, leaves, siliques, flowers, pollen and stems.3 Publications

    Gene expression databases

    ExpressionAtlasiO22141 differential
    GenevisibleiO22141 AT

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi3702.AT2G45310.1

    Structurei

    3D structure databases

    ProteinModelPortaliO22141
    SMRiO22141
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Compositional biasi222 – 225Poly-Ser4

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiENOG410IU3F Eukaryota
    COG0451 LUCA
    HOGENOMiHOG000168000
    InParanoidiO22141
    KOiK08679
    OMAiCFADISK
    OrthoDBiEOG09360AZR
    PhylomeDBiO22141

    Family and domain databases

    InterProiView protein in InterPro
    IPR001509 Epimerase_deHydtase
    IPR036291 NAD(P)-bd_dom_sf
    IPR008089 Nuc_sugar_epim
    PfamiView protein in Pfam
    PF01370 Epimerase, 1 hit
    PRINTSiPR01713 NUCEPIMERASE
    SUPFAMiSSF51735 SSF51735, 1 hit

    Sequencei

    Sequence statusi: Complete.

    O22141-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MSRLDDIPSS PGKFKMEKSS YLHRLRFQSS LTKFAFFSFF LLCLISLLFL
    60 70 80 90 100
    RSPPSINPSS PSDPSRRSLR TNTYGGPAWE KRLRSSARIR TSTNNGITVL
    110 120 130 140 150
    VTGAAGFVGT HVSAALKRRG DGVIGLDNFN DYYDPSLKRA RRALLERSGI
    160 170 180 190 200
    FIVEGDINDV ELLRKLFKIV SFTHVMHLAA QAGVRYAMEN PSSYVHSNIA
    210 220 230 240 250
    GFVNLLEICK SVNPQPAIVW ASSSSVYGLN TKVPFSEKDK TDQPASLYAA
    260 270 280 290 300
    TKKAGEEIAH TYNHIYGLSL TGLRFFTVYG PWGRPDMAYF FFTKDILKGK
    310 320 330 340 350
    SISIFESANH GTVARDFTYI DDIVKGCLAA LDTAEKSTGS GGKKRGPAQL
    360 370 380 390 400
    RVFNLGNTSP VPVSDLVRIL ERQLKVKAKK NLIKMPRNGD VPFTHANISL
    410 420 430
    AQRELGYKPT TDLQTGLKKF VRWYLSYYSG DKKAAAR
    Length:437
    Mass (Da):48,536
    Last modified:January 1, 1998 - v1
    Checksum:iFF8D60C56550CB41
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AY594693 mRNA Translation: AAT06796.1
    AC002387 Genomic DNA Translation: AAB82632.1
    CP002685 Genomic DNA Translation: AEC10537.1
    BT005652 mRNA Translation: AAO64072.1
    BT004225 mRNA Translation: AAO42241.1
    PIRiA84889
    RefSeqiNP_182056.1, NM_130094.2
    UniGeneiAt.66419

    Genome annotation databases

    EnsemblPlantsiAT2G45310.1; AT2G45310.1; AT2G45310
    GeneIDi819139
    GrameneiAT2G45310.1; AT2G45310.1; AT2G45310
    KEGGiath:AT2G45310

    Similar proteinsi

    Entry informationi

    Entry nameiGAE4_ARATH
    AccessioniPrimary (citable) accession number: O22141
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2007
    Last sequence update: January 1, 1998
    Last modified: May 23, 2018
    This is version 116 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Cookie policy

    We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health