Alternative oxidase 2, mitochondrial precursor - Arabidopsis thaliana (Mouse-ear cress)

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O22049 (AOX2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 92. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Alternative oxidase 2, mitochondrial
EC=1.-.-.-

Gene names

Name:	AOX2
Ordered Locus Names:	At5g64210
ORF Names:	MSJ1.5

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	353 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes cyanide-resistant oxygen consumption. May increase respiration when the cytochrome respiratory pathway is restricted, or in response to low temperatures By similarity.
Cofactor	Binds 2 iron ions per subunit.
Subunit structure	Homodimer; disulfide-linked Probable.
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein Probable. Note: Mitochondrial, possibly in the inner surface of the inner mitochondrial membrane. Ref.2
Tissue specificity	Maximally expressed in dry seeds. Ref.2
Developmental stage	Found predominantly during early stages of germination with maximum level at 12 hours after imbibition. Ref.2
Induction	Antimycin A has no effect on expression. Ref.2
Sequence similarities	Belongs to the alternative oxidase family.
Sequence caution	The sequence BAB09852.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide Transmembrane Transmembrane helix
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW respiratory gaseous exchange Inferred from electronic annotation. Source: InterPro transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 21

Mitochondrion Potential

Chain

22 – 353

332

Alternative oxidase 2, mitochondrial

PRO_0000001734

Regions

Transmembrane

178 – 198

Helical; Potential

Transmembrane

240 – 260

Helical; Potential

Sites

Metal binding

182

Iron Potential

Metal binding

221

Iron Potential

Metal binding

224

Iron Potential

Metal binding

273

Iron Potential

Metal binding

323

Iron Potential

Metal binding

326

Iron Potential

Amino acid modifications

Disulfide bond

126

Interchain Potential

Sequences

Sequence

Length

Mass (Da)

Tools

O22049 [UniParc].

Last modified May 27, 2002. Version 2.
Checksum: DF7ACB113E57A133
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FASTA

353

40,087

        10         20         30         40         50         60 
MSQLITKAAL RVLLVCGRGN CNMFVSSVSS TSVMKSPYEI TAPMRIHDWC GGFGDFKIGS 

        70         80         90        100        110        120 
KHVQGNFNLR WMGMSSASAM EKKDENLTVK KGQNGGGSVA VPSYWGIETA KMKITRKDGS 

       130        140        150        160        170        180 
DWPWNCFMPW ETYQANLSID LKKHHVPKNI ADKVAYRIVK LLRIPTDIFF QRRYGCRAMM 

       190        200        210        220        230        240 
LETVAAVPGM VGGMLLHLKS IRKFEHSGGW IKALLEEAEN ERMHLMTMME LVKPKWYERL 

       250        260        270        280        290        300 
LVMLVQGIFF NSFFVCYVIS PRLAHRVVGY LEEEAIHSYT EFLKDIDNGK IENVAAPAIA 

       310        320        330        340        350 
IDYWRLPKDA TLKDVVTVIR ADEAHHRDVN HFASDIRNQG KELREAAAPI GYH

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the gene family for alternative oxidase from Arabidopsis thaliana." Saisho D., Nambara E., Naito S., Tsutsumi N., Hirai A., Nakazono M. Plant Mol. Biol. 35:585-596(1997) [PubMed: 9349280] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: cv. Columbia. Tissue: Leaf and Stem.
[2]	"The gene for alternative oxidase-2 (AOX2) from Arabidopsis thaliana consists of five exons unlike other AOX genes and is transcribed at an early stage during germination." Saisho D., Nakazono M., Lee K.-H., Tsutsumi N., Akita S., Hirai A. Genes Genet. Syst. 76:89-97(2001) [PubMed: 11434463] [Abstract] Cited for: SEQUENCE REVISION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
[3]	"Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence features of the regions of 1,191,918 bp covered by seventeen physically assigned P1 clones." Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N., Tabata S. DNA Res. 4:401-414(1997) [PubMed: 9501997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[5]	Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W., Redman J.C., Wu H.C., Utterback T., Town C.D. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"A revised model of the active site of alternative oxidase." Andersson M.E., Nordlund P. FEBS Lett. 449:17-22(1999) [PubMed: 10225419] [Abstract] Cited for: IRON-BINDING SITES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB003176 Genomic DNA. Translation: BAA22636.2. AB008268 Genomic DNA. Translation: BAB09852.1. Sequence problems. CP002688 Genomic DNA. Translation: AED97855.1. DQ056738 mRNA. Translation: AAY78882.1.
IPI	IPI00542540.
RefSeq	NP_201226.2. NM_125817.2.
UniGene	At.55697.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O22049.
Proteomic databases
PRIDE	O22049.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G64210.1; AT5G64210.1; AT5G64210.
GeneID	836542.
GenomeReviews	Gene locus AT5G64210 in contig BA000015_GR.
KEGG	ath:AT5G64210.
NMPDR	fig\|3702.1.peg.28479.
Organism-specific databases
GeneFarm	1754. 131.
TAIR	At5g64210.
Phylogenomic databases
eggNOG	euNOG04325.
GeneTree	EPGT00050000014563.
HOGENOM	HBG597538.
InParanoid	O22049.
OMA	KVAYRIV.
PhylomeDB	O22049.
ProtClustDB	PLN02478.
Gene expression databases
ArrayExpress	O22049.
Genevestigator	O22049.
GermOnline	AT5G64210. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR002680. AOX. [Graphical view]
KO	K00540.
Pfam	PF01786. AOX. 1 hit. [Graphical view]
PIRSF	PIRSF005229. AOX. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AOX2_ARATH

Accession

Primary (citable) accession number: O22049
Secondary accession number(s): Q4PS98

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 27, 2002
Last modified:	December 14, 2011
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents