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Protein

Mitogen-activated protein kinase kinase kinase 3

Gene

ANP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in cortical microtubules organization and stabilization by regulating the phosphorylation state of microtubule-associated proteins such as MAP65-1.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei97ATPPROSITE-ProRule annotation1
Active sitei196Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi74 – 82ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • cortical microtubule organization Source: UniProtKB
  • protein autophosphorylation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase kinase 3 (EC:2.7.11.25)
Alternative name(s):
Arabidopsis NPK1-related protein kinase 3
Gene namesi
Name:ANP3
Ordered Locus Names:At3g06030
ORF Names:F24F17.1, F2O10.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G06030.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • cytoplasm Source: GO_Central
  • microtubule Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000862721 – 651Mitogen-activated protein kinase kinase kinase 3Add BLAST651

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki108Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki110Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiO22042.
PRIDEiO22042.

PTM databases

iPTMnetiO22042.

Expressioni

Tissue specificityi

Expressed in roots and flowers.1 Publication

Gene expression databases

GenevisibleiO22042. AT.

Interactioni

Subunit structurei

Interacts with NACK2 and MKK6.1 Publication

Protein-protein interaction databases

BioGridi5109. 3 interactors.
IntActiO22042. 2 interactors.
STRINGi3702.AT3G06030.1.

Structurei

3D structure databases

ProteinModelPortaliO22042.
SMRiO22042.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 330Protein kinasePROSITE-ProRule annotationAdd BLAST263

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili105 – 130Sequence analysisAdd BLAST26
Coiled coili618 – 641Sequence analysisAdd BLAST24

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0198. Eukaryota.
ENOG410XQGS. LUCA.
HOGENOMiHOG000033953.
InParanoidiO22042.
OMAiEFHNAMN.
OrthoDBiEOG09360531.
PhylomeDBiO22042.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O22042-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQDILGSVRR SLVFRSSLAG DDGTSGGGLS GFVGKINSSI RSSRIGLFSK
60 70 80 90 100
PPPGLPAPRK EEAPSIRWRK GELIGCGAFG RVYMGMNLDS GELLAIKQVL
110 120 130 140 150
IAPSSASKEK TQGHIRELEE EVQLLKNLSH PNIVRYLGTV RESDSLNILM
160 170 180 190 200
EFVPGGSISS LLEKFGSFPE PVIIMYTKQL LLGLEYLHNN GIMHRDIKGA
210 220 230 240 250
NILVDNKGCI RLADFGASKK VVELATVNGA KSMKGTPYWM APEVILQTGH
260 270 280 290 300
SFSADIWSVG CTVIEMATGK PPWSEQYQQF AAVLHIGRTK AHPPIPEDLS
310 320 330 340 350
PEAKDFLMKC LHKEPSLRLS ATELLQHPFV TGKRQEPYPA YRNSLTECGN
360 370 380 390 400
PITTQGMNVR SSINSLIRRS TCSGLKDVCE LGSLRSSIIY PQKSNNSGFG
410 420 430 440 450
WRDGDSDDLC QTDMDDLCNI ESVRNNVLSQ STDLNKSFNP MCDSTDNWSC
460 470 480 490 500
KFDESPKVMK SKSNLLSYQA SQLQTGVPCD EETSLTFAGG SSVAEDDYKG
510 520 530 540 550
TELKIKSFLD EKAQDLKRLQ TPLLEEFHNA MNPGIPQGAL GDTNIYNLPN
560 570 580 590 600
LPSISKTPKR LPSRRLSAIS DAMPSPLKSS KRTLNTSRVM QSGTEPTQVN
610 620 630 640 650
ESTKKGVNNS RCFSEIRRKW EEELYEELER HRENLRHAGA GGKTPLSGHK

G
Length:651
Mass (Da):71,654
Last modified:January 1, 1998 - v1
Checksum:iE8FDF683C7E31268
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000799 mRNA. Translation: BAA21857.1.
AC013454 Genomic DNA. Translation: AAF23214.1.
AC068073 Genomic DNA. Translation: AAF66131.1.
CP002686 Genomic DNA. Translation: AEE74332.1.
AY069917 mRNA. Translation: AAL47465.1.
BT001024 mRNA. Translation: AAN46778.1.
RefSeqiNP_187254.1. NM_111477.4.
UniGeneiAt.23105.

Genome annotation databases

EnsemblPlantsiAT3G06030.1; AT3G06030.1; AT3G06030.
GeneIDi819774.
GrameneiAT3G06030.1; AT3G06030.1; AT3G06030.
KEGGiath:AT3G06030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000799 mRNA. Translation: BAA21857.1.
AC013454 Genomic DNA. Translation: AAF23214.1.
AC068073 Genomic DNA. Translation: AAF66131.1.
CP002686 Genomic DNA. Translation: AEE74332.1.
AY069917 mRNA. Translation: AAL47465.1.
BT001024 mRNA. Translation: AAN46778.1.
RefSeqiNP_187254.1. NM_111477.4.
UniGeneiAt.23105.

3D structure databases

ProteinModelPortaliO22042.
SMRiO22042.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi5109. 3 interactors.
IntActiO22042. 2 interactors.
STRINGi3702.AT3G06030.1.

PTM databases

iPTMnetiO22042.

Proteomic databases

PaxDbiO22042.
PRIDEiO22042.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G06030.1; AT3G06030.1; AT3G06030.
GeneIDi819774.
GrameneiAT3G06030.1; AT3G06030.1; AT3G06030.
KEGGiath:AT3G06030.

Organism-specific databases

TAIRiAT3G06030.

Phylogenomic databases

eggNOGiKOG0198. Eukaryota.
ENOG410XQGS. LUCA.
HOGENOMiHOG000033953.
InParanoidiO22042.
OMAiEFHNAMN.
OrthoDBiEOG09360531.
PhylomeDBiO22042.

Miscellaneous databases

PROiO22042.

Gene expression databases

GenevisibleiO22042. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiM3K3_ARATH
AccessioniPrimary (citable) accession number: O22042
Secondary accession number(s): Q9SFG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.