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Protein

Mitogen-activated protein kinase kinase kinase 3

Gene

ANP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in cortical microtubules organization and stabilization by regulating the phosphorylation state of microtubule-associated proteins such as MAP65-1.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei97ATPPROSITE-ProRule annotation1
Active sitei196Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi74 – 82ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase kinase 3 (EC:2.7.11.25)
Alternative name(s):
Arabidopsis NPK1-related protein kinase 3
Gene namesi
Name:ANP3
Ordered Locus Names:At3g06030
ORF Names:F24F17.1, F2O10.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G06030
TAIRilocus:2080394 AT3G06030

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000862721 – 651Mitogen-activated protein kinase kinase kinase 3Add BLAST651

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki108Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki110Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiO22042
PRIDEiO22042

PTM databases

iPTMnetiO22042

Expressioni

Tissue specificityi

Expressed in roots and flowers.1 Publication

Gene expression databases

ExpressionAtlasiO22042 baseline and differential
GenevisibleiO22042 AT

Interactioni

Subunit structurei

Interacts with NACK2 and MKK6.1 Publication

Protein-protein interaction databases

BioGridi5109, 3 interactors
IntActiO22042, 2 interactors
STRINGi3702.AT3G06030.1

Structurei

3D structure databases

ProteinModelPortaliO22042
SMRiO22042
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini68 – 330Protein kinasePROSITE-ProRule annotationAdd BLAST263

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili105 – 130Sequence analysisAdd BLAST26
Coiled coili618 – 641Sequence analysisAdd BLAST24

Sequence similaritiesi

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0198 Eukaryota
ENOG410XQGS LUCA
HOGENOMiHOG000033953
InParanoidiO22042
OMAiSCKFDES
OrthoDBiEOG09360531
PhylomeDBiO22042

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

O22042-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQDILGSVRR SLVFRSSLAG DDGTSGGGLS GFVGKINSSI RSSRIGLFSK
60 70 80 90 100
PPPGLPAPRK EEAPSIRWRK GELIGCGAFG RVYMGMNLDS GELLAIKQVL
110 120 130 140 150
IAPSSASKEK TQGHIRELEE EVQLLKNLSH PNIVRYLGTV RESDSLNILM
160 170 180 190 200
EFVPGGSISS LLEKFGSFPE PVIIMYTKQL LLGLEYLHNN GIMHRDIKGA
210 220 230 240 250
NILVDNKGCI RLADFGASKK VVELATVNGA KSMKGTPYWM APEVILQTGH
260 270 280 290 300
SFSADIWSVG CTVIEMATGK PPWSEQYQQF AAVLHIGRTK AHPPIPEDLS
310 320 330 340 350
PEAKDFLMKC LHKEPSLRLS ATELLQHPFV TGKRQEPYPA YRNSLTECGN
360 370 380 390 400
PITTQGMNVR SSINSLIRRS TCSGLKDVCE LGSLRSSIIY PQKSNNSGFG
410 420 430 440 450
WRDGDSDDLC QTDMDDLCNI ESVRNNVLSQ STDLNKSFNP MCDSTDNWSC
460 470 480 490 500
KFDESPKVMK SKSNLLSYQA SQLQTGVPCD EETSLTFAGG SSVAEDDYKG
510 520 530 540 550
TELKIKSFLD EKAQDLKRLQ TPLLEEFHNA MNPGIPQGAL GDTNIYNLPN
560 570 580 590 600
LPSISKTPKR LPSRRLSAIS DAMPSPLKSS KRTLNTSRVM QSGTEPTQVN
610 620 630 640 650
ESTKKGVNNS RCFSEIRRKW EEELYEELER HRENLRHAGA GGKTPLSGHK

G
Length:651
Mass (Da):71,654
Last modified:January 1, 1998 - v1
Checksum:iE8FDF683C7E31268
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000799 mRNA Translation: BAA21857.1
AC013454 Genomic DNA Translation: AAF23214.1
AC068073 Genomic DNA Translation: AAF66131.1
CP002686 Genomic DNA Translation: AEE74332.1
AY069917 mRNA Translation: AAL47465.1
BT001024 mRNA Translation: AAN46778.1
RefSeqiNP_187254.1, NM_111477.4
UniGeneiAt.23105

Genome annotation databases

EnsemblPlantsiAT3G06030.1; AT3G06030.1; AT3G06030
GeneIDi819774
GrameneiAT3G06030.1; AT3G06030.1; AT3G06030
KEGGiath:AT3G06030

Similar proteinsi

Entry informationi

Entry nameiM3K3_ARATH
AccessioniPrimary (citable) accession number: O22042
Secondary accession number(s): Q9SFG7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: January 1, 1998
Last modified: April 25, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health