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Protein

Mitogen-activated protein kinase kinase kinase 3

Gene

ANP3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in cortical microtubules organization and stabilization by regulating the phosphorylation state of microtubule-associated proteins such as MAP65-1.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei97 – 971ATPPROSITE-ProRule annotation
Active sitei196 – 1961Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi74 – 829ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • cortical microtubule organization Source: UniProtKB
  • protein autophosphorylation Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT3G06030-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase kinase 3 (EC:2.7.11.25)
Alternative name(s):
Arabidopsis NPK1-related protein kinase 3
Gene namesi
Name:ANP3
Ordered Locus Names:At3g06030
ORF Names:F24F17.1, F2O10.1
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G06030.

Subcellular locationi

GO - Cellular componenti

  • apoplast Source: TAIR
  • cytoplasm Source: GO_Central
  • microtubule Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 651651Mitogen-activated protein kinase kinase kinase 3PRO_0000086272Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki108 – 108Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki110 – 110Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiO22042.
PRIDEiO22042.

PTM databases

iPTMnetiO22042.

Expressioni

Tissue specificityi

Expressed in roots and flowers.1 Publication

Gene expression databases

GenevisibleiO22042. AT.

Interactioni

Subunit structurei

Interacts with NACK2 and MKK6.1 Publication

Protein-protein interaction databases

BioGridi5109. 3 interactions.
IntActiO22042. 2 interactions.
STRINGi3702.AT3G06030.1.

Structurei

3D structure databases

ProteinModelPortaliO22042.
SMRiO22042. Positions 68-342.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini68 – 330263Protein kinasePROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili105 – 13026Sequence analysisAdd
BLAST
Coiled coili618 – 64124Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0198. Eukaryota.
ENOG410XQGS. LUCA.
HOGENOMiHOG000033953.
InParanoidiO22042.
OMAiEFHNAMN.
PhylomeDBiO22042.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O22042-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQDILGSVRR SLVFRSSLAG DDGTSGGGLS GFVGKINSSI RSSRIGLFSK
60 70 80 90 100
PPPGLPAPRK EEAPSIRWRK GELIGCGAFG RVYMGMNLDS GELLAIKQVL
110 120 130 140 150
IAPSSASKEK TQGHIRELEE EVQLLKNLSH PNIVRYLGTV RESDSLNILM
160 170 180 190 200
EFVPGGSISS LLEKFGSFPE PVIIMYTKQL LLGLEYLHNN GIMHRDIKGA
210 220 230 240 250
NILVDNKGCI RLADFGASKK VVELATVNGA KSMKGTPYWM APEVILQTGH
260 270 280 290 300
SFSADIWSVG CTVIEMATGK PPWSEQYQQF AAVLHIGRTK AHPPIPEDLS
310 320 330 340 350
PEAKDFLMKC LHKEPSLRLS ATELLQHPFV TGKRQEPYPA YRNSLTECGN
360 370 380 390 400
PITTQGMNVR SSINSLIRRS TCSGLKDVCE LGSLRSSIIY PQKSNNSGFG
410 420 430 440 450
WRDGDSDDLC QTDMDDLCNI ESVRNNVLSQ STDLNKSFNP MCDSTDNWSC
460 470 480 490 500
KFDESPKVMK SKSNLLSYQA SQLQTGVPCD EETSLTFAGG SSVAEDDYKG
510 520 530 540 550
TELKIKSFLD EKAQDLKRLQ TPLLEEFHNA MNPGIPQGAL GDTNIYNLPN
560 570 580 590 600
LPSISKTPKR LPSRRLSAIS DAMPSPLKSS KRTLNTSRVM QSGTEPTQVN
610 620 630 640 650
ESTKKGVNNS RCFSEIRRKW EEELYEELER HRENLRHAGA GGKTPLSGHK

G
Length:651
Mass (Da):71,654
Last modified:January 1, 1998 - v1
Checksum:iE8FDF683C7E31268
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000799 mRNA. Translation: BAA21857.1.
AC013454 Genomic DNA. Translation: AAF23214.1.
AC068073 Genomic DNA. Translation: AAF66131.1.
CP002686 Genomic DNA. Translation: AEE74332.1.
AY069917 mRNA. Translation: AAL47465.1.
BT001024 mRNA. Translation: AAN46778.1.
RefSeqiNP_187254.1. NM_111477.3.
UniGeneiAt.23105.

Genome annotation databases

EnsemblPlantsiAT3G06030.1; AT3G06030.1; AT3G06030.
GeneIDi819774.
GrameneiAT3G06030.1; AT3G06030.1; AT3G06030.
KEGGiath:AT3G06030.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000799 mRNA. Translation: BAA21857.1.
AC013454 Genomic DNA. Translation: AAF23214.1.
AC068073 Genomic DNA. Translation: AAF66131.1.
CP002686 Genomic DNA. Translation: AEE74332.1.
AY069917 mRNA. Translation: AAL47465.1.
BT001024 mRNA. Translation: AAN46778.1.
RefSeqiNP_187254.1. NM_111477.3.
UniGeneiAt.23105.

3D structure databases

ProteinModelPortaliO22042.
SMRiO22042. Positions 68-342.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi5109. 3 interactions.
IntActiO22042. 2 interactions.
STRINGi3702.AT3G06030.1.

PTM databases

iPTMnetiO22042.

Proteomic databases

PaxDbiO22042.
PRIDEiO22042.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G06030.1; AT3G06030.1; AT3G06030.
GeneIDi819774.
GrameneiAT3G06030.1; AT3G06030.1; AT3G06030.
KEGGiath:AT3G06030.

Organism-specific databases

TAIRiAT3G06030.

Phylogenomic databases

eggNOGiKOG0198. Eukaryota.
ENOG410XQGS. LUCA.
HOGENOMiHOG000033953.
InParanoidiO22042.
OMAiEFHNAMN.
PhylomeDBiO22042.

Enzyme and pathway databases

BioCyciARA:AT3G06030-MONOMER.

Miscellaneous databases

PROiO22042.

Gene expression databases

GenevisibleiO22042. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Possible involvement of differential splicing in regulation of the activity of Arabidopsis ANP1 that is related to mitogen-activated protein kinase kinase kinases (MAPKKKs)."
    Nishihama R., Banno H., Kawahara E., Irie K., Machida Y.
    Plant J. 12:39-48(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Multidimensional protein identification technology (MudPIT) analysis of ubiquitinated proteins in plants."
    Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K.
    Mol. Cell. Proteomics 6:601-610(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-108 AND LYS-110.
    Strain: cv. Landsberg erecta.
  6. "Arabidopsis homologs of nucleus- and phragmoplast-localized kinase 2 and 3 and mitogen-activated protein kinase 4 are essential for microtubule organization."
    Beck M., Komis G., Mueller J., Menzel D., Samaj J.
    Plant Cell 22:755-771(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "AtMPK4 is required for male-specific meiotic cytokinesis in Arabidopsis."
    Zeng Q., Chen J.G., Ellis B.E.
    Plant J. 67:895-906(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NACK2 AND MKK6.

Entry informationi

Entry nameiM3K3_ARATH
AccessioniPrimary (citable) accession number: O22042
Secondary accession number(s): Q9SFG7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: January 1, 1998
Last modified: July 6, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.