Reviewed, UniProtKB/Swiss-Prot O22040 (ANP1_ARATH)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase kinase kinase ANP1
    EC=2.7.11.25
Alternative name(s):
    Arabidopsis NPK1-related kinase 1

Gene names

Name:	ANP1
Ordered Locus Names:	At1g09000
ORF Names:	F7G19.13

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	666 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in an oxidative stress-mediated signaling cascade that phosphorylates downstream MAP kinases MPK3 and MPK6. May suppress auxin signaling that promotes cell cycle. Functionally redundant to ANP2 and ANP3 in the positive regulation of cytokinesis. Ref.5 Ref.6
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Tissue specificity	Expressed in roots, inflorescence stems, flower buds and flowers. Low amount in rosette and cauline leaves. Ref.1
Miscellaneous	The protein kinase activity of isoform 1S is higher than that of isoform 1L.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence AAB70419.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	flower development Ref.6 Inferred from genetic interaction. Source: TAIR protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro response to oxidative stress Ref.5 Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1L (identifier: O22040-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1S (identifier: O22040-2)

The sequence of this isoform differs from the canonical sequence as follows:
     372-376: VGDMC → MMRIS
     377-666: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 666	666	Mitogen-activated protein kinase kinase kinase ANP1		PRO_0000086270
Regions
	Domain	69 – 331	263	Protein kinase
	Nucleotide binding	75 – 83	9	ATP By similarity
	Coiled coil	101 – 131	31	Potential
	Coiled coil	620 – 643	24	Potential
Sites
	Active site	197	1	Proton acceptor By similarity
	Binding site	98	1	ATP By similarity
Natural variations
	Alternative sequence	372 – 376	5	VGDMC → MMRIS in isoform 1S.		VSP_010124
	Alternative sequence	377 – 666	290	Missing in isoform 1S.		VSP_010125
Experimental info
	Mutagenesis	98	1	K → M: Loss of kinase activity. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1L [UniParc]. Last modified April 26, 2004. Version 2. Checksum: 588C6339CDC58C18	FASTA	666	73,470
	10 20 30 40 50 60 MQDFFGSVRR SLVFRPSSDD DNQENQPPFP GVLADKITSC IRKSKIFIKP SFSPPPPANT 70 80 90 100 110 120 VDMAPPISWR KGQLIGRGAF GTVYMGMNLD SGELLAVKQV LIAANFASKE KTQAHIQELE 130 140 150 160 170 180 EEVKLLKNLS HPNIVRYLGT VREDDTLNIL LEFVPGGSIS SLLEKFGPFP ESVVRTYTRQ 190 200 210 220 230 240 LLLGLEYLHN HAIMHRDIKG ANILVDNKGC IKLADFGASK QVAELATMTG AKSMKGTPYW 250 260 270 280 290 300 MAPEVILQTG HSFSADIWSV GCTVIEMVTG KAPWSQQYKE VAAIFFIGTT KSHPPIPDTL 310 320 330 340 350 360 SSDAKDFLLK CLQEVPNLRP TASELLKHPF VMGKHKESAS TDLGSVLNNL STPLPLQINN 370 380 390 400 410 420 TKSTPDSTCD DVGDMCNFGS LNYSLVDPVK SIQNKNLWQQ NDNGGDEDDM CLIDDENFLT 430 440 450 460 470 480 FDGEMSSTLE KDCHLKKSCD DISDMSIALK SKFDESPGNG EKESTMSMEC DQPSYSEDDD 490 500 510 520 530 540 ELTESKIKAF LDEKAADLKK LQTPLYEEFY NSLITFSPSC MESNLSNSKR EDTARGFLKL 550 560 570 580 590 600 PPKSRSPSRG PLGGSPSRAT DATSCSKSPG SGGSRELNIN NGGDEASQDG VSARVTDWRG 610 620 630 640 650 660 LVVDTKQELS QCVALSEIEK KWKEELDQEL ERKRQEIMRQ AGLGSSPRDR GMSRQREKSR FASPGK
	Isoform 1S. Checksum: F23B0B5218673456	FASTA	376	41,385
	10 20 30 40 50 60 MQDFFGSVRR SLVFRPSSDD DNQENQPPFP GVLADKITSC IRKSKIFIKP SFSPPPPANT 70 80 90 100 110 120 VDMAPPISWR KGQLIGRGAF GTVYMGMNLD SGELLAVKQV LIAANFASKE KTQAHIQELE 130 140 150 160 170 180 EEVKLLKNLS HPNIVRYLGT VREDDTLNIL LEFVPGGSIS SLLEKFGPFP ESVVRTYTRQ 190 200 210 220 230 240 LLLGLEYLHN HAIMHRDIKG ANILVDNKGC IKLADFGASK QVAELATMTG AKSMKGTPYW 250 260 270 280 290 300 MAPEVILQTG HSFSADIWSV GCTVIEMVTG KAPWSQQYKE VAAIFFIGTT KSHPPIPDTL 310 320 330 340 350 360 SSDAKDFLLK CLQEVPNLRP TASELLKHPF VMGKHKESAS TDLGSVLNNL STPLPLQINN 370 TKSTPDSTCD DMMRIS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Possible involvement of differential splicing in regulation of the activity of Arabidopsis ANP1 that is related to mitogen-activated protein kinase kinase kinases (MAPKKKs)." Nishihama R., Banno H., Kawahara E., Irie K., Machida Y. Plant J. 12:39-48(1997) [PubMed: 9263451] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1S AND 1L), TISSUE SPECIFICITY. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed: 11910074] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1L). Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1L). Strain: cv. Columbia.
[5]	"Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants." Kovtun Y., Chiu W.-L., Tena G., Sheen J. Proc. Natl. Acad. Sci. U.S.A. 97:2940-2945(2000) [PubMed: 10717008] [Abstract] Cited for: FUNCTION, ENZYME REGULATION, MUTAGENESIS OF LYS-98.
[6]	"An Arabidopsis mitogen-activated protein kinase kinase kinase gene family encodes essential positive regulators of cytokinesis." Krysan P.J., Jester P.J., Gottwald J.R., Sussman M.R. Plant Cell 14:1109-1120(2002) [PubMed: 12034900] [Abstract] Cited for: FUNCTION.
[7]	"Mitogen-activated protein kinase cascades in plants: a new nomenclature." MAPK group Trends Plant Sci. 7:301-308(2002) [PubMed: 12119167] [Abstract] Cited for: NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB000796 mRNA. Translation: BAA21854.1. AB000797 mRNA. Translation: BAA21855.1. AC000106 Genomic DNA. Translation: AAB70419.1. Sequence problems. AK117282 mRNA. Translation: BAC41954.1. BT005949 mRNA. Translation: AAO64884.1.
IPI	IPI00531470. IPI00538145.
PIR	H86221.
RefSeq	NP_563832.2.
UniGene	At.70237
3D structure databases
HSSP	HSSP built from PDB template 1AQ1 based on UniProtKB P24941.
ModBase	Search...
Proteomic databases
PRIDE	O22040.
Genome annotation databases
GeneID	837421.
GenomeReviews	Gene locus AT1G09000 in contig CT485782_GR.
KEGG	ath:AT1G09000.
NMPDR	fig|3702.1.peg.1117.
Organism-specific databases
GeneFarm	898. 89.
TAIR	At1g09000.
Phylogenomic databases
OMA	O22040. IDSHEAS.
Enzyme and pathway databases
BRENDA	2.7.11.25. 302.
Gene expression databases
ArrayExpress	O22040.
GermOnline	AT1G09000. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR015748. MAPKKK3. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
PANTHER	PTHR22986:SF64. MAPKKK3. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ANP1_ARATH

Accession

Primary (citable) accession number: O22040
Secondary accession number(s): O04030, O22039, Q8GZ05

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	April 26, 2004
Last modified:	June 16, 2009
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)