Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mitogen-activated protein kinase kinase kinase ANP1

Gene

ANP1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in an oxidative stress-mediated signaling cascade that phosphorylates downstream MAP kinases MPK3 and MPK6. May suppress auxin signaling that promotes cell cycle. Functionally redundant to ANP2 and ANP3 in the positive regulation of cytokinesis.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei98 – 981ATPPROSITE-ProRule annotation
Active sitei197 – 1971Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi75 – 839ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase kinase kinase activity Source: TAIR
  • protein serine/threonine kinase activity Source: TAIR
  • receptor signaling protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

  • protein autophosphorylation Source: TAIR
  • response to oxidative stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G09000-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase kinase kinase ANP1 (EC:2.7.11.25)
Alternative name(s):
Arabidopsis NPK1-related kinase 1
Gene namesi
Name:ANP1
Ordered Locus Names:At1g09000
ORF Names:F7G19.13
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G09000.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi98 – 981K → M: Loss of kinase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 666666Mitogen-activated protein kinase kinase kinase ANP1PRO_0000086270Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki109 – 109Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki111 – 111Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiO22040.
PRIDEiO22040.

Expressioni

Tissue specificityi

Expressed in roots, inflorescence stems, flower buds and flowers. Low amount in rosette and cauline leaves.1 Publication

Gene expression databases

GenevisibleiO22040. AT.

Interactioni

Protein-protein interaction databases

BioGridi22662. 2 interactions.
STRINGi3702.AT1G09000.1.

Structurei

3D structure databases

ProteinModelPortaliO22040.
SMRiO22040. Positions 33-347.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini69 – 331263Protein kinasePROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili101 – 13131Sequence analysisAdd
BLAST
Coiled coili620 – 64324Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG0198. Eukaryota.
ENOG410XQGS. LUCA.
HOGENOMiHOG000033953.
InParanoidiO22040.
OMAiFYNTANA.
PhylomeDBiO22040.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1L (identifier: O22040-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQDFFGSVRR SLVFRPSSDD DNQENQPPFP GVLADKITSC IRKSKIFIKP
60 70 80 90 100
SFSPPPPANT VDMAPPISWR KGQLIGRGAF GTVYMGMNLD SGELLAVKQV
110 120 130 140 150
LIAANFASKE KTQAHIQELE EEVKLLKNLS HPNIVRYLGT VREDDTLNIL
160 170 180 190 200
LEFVPGGSIS SLLEKFGPFP ESVVRTYTRQ LLLGLEYLHN HAIMHRDIKG
210 220 230 240 250
ANILVDNKGC IKLADFGASK QVAELATMTG AKSMKGTPYW MAPEVILQTG
260 270 280 290 300
HSFSADIWSV GCTVIEMVTG KAPWSQQYKE VAAIFFIGTT KSHPPIPDTL
310 320 330 340 350
SSDAKDFLLK CLQEVPNLRP TASELLKHPF VMGKHKESAS TDLGSVLNNL
360 370 380 390 400
STPLPLQINN TKSTPDSTCD DVGDMCNFGS LNYSLVDPVK SIQNKNLWQQ
410 420 430 440 450
NDNGGDEDDM CLIDDENFLT FDGEMSSTLE KDCHLKKSCD DISDMSIALK
460 470 480 490 500
SKFDESPGNG EKESTMSMEC DQPSYSEDDD ELTESKIKAF LDEKAADLKK
510 520 530 540 550
LQTPLYEEFY NSLITFSPSC MESNLSNSKR EDTARGFLKL PPKSRSPSRG
560 570 580 590 600
PLGGSPSRAT DATSCSKSPG SGGSRELNIN NGGDEASQDG VSARVTDWRG
610 620 630 640 650
LVVDTKQELS QCVALSEIEK KWKEELDQEL ERKRQEIMRQ AGLGSSPRDR
660
GMSRQREKSR FASPGK
Length:666
Mass (Da):73,470
Last modified:April 26, 2004 - v2
Checksum:i588C6339CDC58C18
GO
Isoform 1S (identifier: O22040-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     372-376: VGDMC → MMRIS
     377-666: Missing.

Show »
Length:376
Mass (Da):41,385
Checksum:iF23B0B5218673456
GO

Sequence cautioni

The sequence AAB70419.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei372 – 3765VGDMC → MMRIS in isoform 1S. 1 PublicationVSP_010124
Alternative sequencei377 – 666290Missing in isoform 1S. 1 PublicationVSP_010125Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000796 mRNA. Translation: BAA21854.1.
AB000797 mRNA. Translation: BAA21855.1.
AC000106 Genomic DNA. Translation: AAB70419.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28381.1.
AK117282 mRNA. Translation: BAC41954.1.
BT005949 mRNA. Translation: AAO64884.1.
PIRiH86221.
RefSeqiNP_563832.2. NM_100771.3. [O22040-1]
UniGeneiAt.48170.
At.70237.

Genome annotation databases

EnsemblPlantsiAT1G09000.1; AT1G09000.1; AT1G09000. [O22040-1]
GeneIDi837421.
KEGGiath:AT1G09000.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB000796 mRNA. Translation: BAA21854.1.
AB000797 mRNA. Translation: BAA21855.1.
AC000106 Genomic DNA. Translation: AAB70419.1. Sequence problems.
CP002684 Genomic DNA. Translation: AEE28381.1.
AK117282 mRNA. Translation: BAC41954.1.
BT005949 mRNA. Translation: AAO64884.1.
PIRiH86221.
RefSeqiNP_563832.2. NM_100771.3. [O22040-1]
UniGeneiAt.48170.
At.70237.

3D structure databases

ProteinModelPortaliO22040.
SMRiO22040. Positions 33-347.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi22662. 2 interactions.
STRINGi3702.AT1G09000.1.

Proteomic databases

PaxDbiO22040.
PRIDEiO22040.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G09000.1; AT1G09000.1; AT1G09000. [O22040-1]
GeneIDi837421.
KEGGiath:AT1G09000.

Organism-specific databases

TAIRiAT1G09000.

Phylogenomic databases

eggNOGiKOG0198. Eukaryota.
ENOG410XQGS. LUCA.
HOGENOMiHOG000033953.
InParanoidiO22040.
OMAiFYNTANA.
PhylomeDBiO22040.

Enzyme and pathway databases

BioCyciARA:AT1G09000-MONOMER.

Miscellaneous databases

PROiO22040.

Gene expression databases

GenevisibleiO22040. AT.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Possible involvement of differential splicing in regulation of the activity of Arabidopsis ANP1 that is related to mitogen-activated protein kinase kinase kinases (MAPKKKs)."
    Nishihama R., Banno H., Kawahara E., Irie K., Machida Y.
    Plant J. 12:39-48(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1S AND 1L), TISSUE SPECIFICITY.
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1L).
    Strain: cv. Columbia.
  5. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1L).
    Strain: cv. Columbia.
  6. "Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants."
    Kovtun Y., Chiu W.-L., Tena G., Sheen J.
    Proc. Natl. Acad. Sci. U.S.A. 97:2940-2945(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ENZYME REGULATION, MUTAGENESIS OF LYS-98.
  7. "An Arabidopsis mitogen-activated protein kinase kinase kinase gene family encodes essential positive regulators of cytokinesis."
    Krysan P.J., Jester P.J., Gottwald J.R., Sussman M.R.
    Plant Cell 14:1109-1120(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. "Mitogen-activated protein kinase cascades in plants: a new nomenclature."
    MAPK group
    Trends Plant Sci. 7:301-308(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.

Entry informationi

Entry nameiANP1_ARATH
AccessioniPrimary (citable) accession number: O22040
Secondary accession number(s): O04030, O22039, Q8GZ05
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: April 26, 2004
Last modified: July 6, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

The protein kinase activity of isoform 1S is higher than that of isoform 1L.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.