NADH-ubiquinone oxidoreductase 75 kDa subunit

Preferred order of sections

Names and origin

Protein names

Recommended name:
NADH-ubiquinone oxidoreductase 75 kDa subunit
EC=1.6.5.3
EC=1.6.99.3

Alternative name(s):
Complex I-75kD
Short name=CI-75kD
NADH dehydrogenase subunit 11

Gene names

Name:

NAD11

Encoded on

Mitochondrion

Organism

Reclinomonas americana

Taxonomic identifier

48483 [NCBI]

Taxonomic lineage

Eukaryota › Jakobida › Histionidae › Reclinomonas

Protein attributes

Sequence length	691 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity.
Catalytic activity	NADH + ubiquinone = NAD⁺ + ubiquinol. NADH + acceptor = NAD⁺ + reduced acceptor.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity. Binds 2 4Fe-4S clusters per subunit By similarity.
Subunit structure	Complex I is composed of about 30 different subunits By similarity.
Subcellular location	Mitochondrion inner membrane By similarity. Note: Matrix and cytoplasmic side of the mitochondrial inner membrane By similarity.
Sequence similarities	Belongs to the complex I 75 kDa subunit family. Contains 1 2Fe-2S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro
Cellular_component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 691

691

NADH-ubiquinone oxidoreductase 75 kDa subunit

PRO_0000118537

Regions

Domain

1 – 78

78

2Fe-2S ferredoxin-type

Sites

Metal binding

34

1

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

45

1

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

48

1

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

62

1

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

94

1

Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity

Metal binding

98

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

101

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

107

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

146

1

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

149

1

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

152

1

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

196

1

Iron-sulfur 3 (4Fe-4S) By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

O21241 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 1FB45AF558F469E2
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FASTA

691

77,718

        10         20         30         40         50         60 
MVNVFVDGLS VEVKKGATIL QACAQVGIEI PRFCYHERLS IAGNCRMCLV EVEKSPKPVA 

        70         80         90        100        110        120 
SCAMPVMDNM KIFTNTPLVK KAREGVLEFL LVNHPLDCPI CDQGGECDLQ DLTMVYGSDR 

       130        140        150        160        170        180 
GRFHEYKRGV EDKNIGPLVK TVMTRCIHCT RCVRFATEVA GVPDLGTVGR GRDTEISTYI 

       190        200        210        220        230        240 
QKVFNSELSG NVIDLCPVGA LTSKPYAFTA RSWELQSTES IDVSDAIGSN IRIDVRGSEI 

       250        260        270        280        290        300 
MRILPRLNED VNEEWISDKA RFCYDGLKRQ RLNNPIIKEN GQYKTVTWEK AFNFILKNLQ 

       310        320        330        340        350        360 
EIQNSNRIVG VVGNLMDVES ILLFKELLNK LGSSKIYLES STPILQLNDD EKEDQILNNA 

       370        380        390        400        410        420 
DFRNNYISNT PLAKIEESDL CLLIGTNIRL EAPLLNTRIR KRYLQGNYSV YSVGPTNNLT 

       430        440        450        460        470        480 
YNTENLGNDI STLLEISEGR HPFCKKLMKS KKPLIIIGTH VLQRTDGTSI IELVKTLFKY 

       490        500        510        520        530        540 
TQIKTSNWNG FNILHTSASS VGALDLGIGS TKRYSEKISN SKIEKHFIYL LGADEIRIEN 

       550        560        570        580        590        600 
SKEHFIVYQG HHGDYGANIA DVILPGSAYT EKTATYVNVE GRVQNTKSAF YAPGNAREDW 

       610        620        630        640        650        660 
KIIRALSEVL NKKLPYDSFE DIHTRFMSIA PHLLKVNAIE KNKIVIENSL PFKGLIKNIG 

       670        680        690 
FKPLFNNFYL TNAICRSSQT MAKCSSIYKL N

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References

[1]

"An ancestral mitochondrial DNA resembling a eubacterial genome in miniature."
Lang B.F., Burger G., O'Kelly C.J., Cedergren R., Golding G.B., Lemieux C., Sankoff D., Turmel M., Gray M.W.
Nature 387:493-497(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 50394.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF007261 Genomic DNA. Translation: AAD11868.1.
PIR	S78135.
RefSeq	NP_044753.1. NC_001823.1.
3D structure databases
ProteinModelPortal	O21241.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	801135.
Family and domain databases
Gene3D	3.10.20.30. 1 hit.
InterPro	IPR001041. 2Fe-2S_ferredoxin-type. IPR012675. Beta-grasp_dom. IPR006656. Mopterin_OxRdtase. IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS. IPR010228. NADH_UbQ_OxRdtase_Gsu. IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd. IPR015405. NuoG_C. [Graphical view]
Pfam	PF09326. DUF1982. 1 hit. PF00111. Fer2. 1 hit. PF00384. Molybdopterin. 1 hit. PF10588. NADH-G_4Fe-4S_3. 1 hit. [Graphical view]
SMART	SM00929. NADH-G_4Fe-4S_3. 1 hit. [Graphical view]
SUPFAM	SSF54292. Ferredoxin. 1 hit.
TIGRFAMs	TIGR01973. NuoG. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. False negative. PS51085. 2FE2S_FER_2. 1 hit. PS00641. COMPLEX1_75K_1. 1 hit. PS00642. COMPLEX1_75K_2. 1 hit. PS00643. COMPLEX1_75K_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDUS1_RECAM

Accession

Primary (citable) accession number: O21241

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Family and domain databases

Entry information

Relevant documents