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O21241 (NDUS1_RECAM) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
NADH-ubiquinone oxidoreductase 75 kDa subunit

EC=1.6.5.3
EC=1.6.99.3
Alternative name(s):
Complex I-75kD
Short name=CI-75kD
NADH dehydrogenase subunit 11
Gene names
Name:NAD11
Encoded onMitochondrion
OrganismReclinomonas americana
Taxonomic identifier48483 [NCBI]
Taxonomic lineageEukaryotaJakobidaHistionidaeReclinomonas

Protein attributes

Sequence length691 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity.

Catalytic activity

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).

NADH + acceptor = NAD+ + reduced acceptor.

Cofactor

Binds 1 2Fe-2S cluster per subunit By similarity.

Binds 2 4Fe-4S clusters per subunit By similarity.

Subunit structure

Complex I is composed of about 30 different subunits By similarity.

Subcellular location

Mitochondrion inner membrane By similarity. Note: Matrix and cytoplasmic side of the mitochondrial inner membrane By similarity.

Sequence similarities

Belongs to the complex I 75 kDa subunit family.

Contains 1 2Fe-2S ferredoxin-type domain.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 691691NADH-ubiquinone oxidoreductase 75 kDa subunit
PRO_0000118537

Regions

Domain1 – 78782Fe-2S ferredoxin-type
Domain215 – 271574Fe-4S Mo/W bis-MGD-type

Sites

Metal binding341Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding451Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding481Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding621Iron-sulfur 1 (2Fe-2S) By similarity
Metal binding941Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
Metal binding981Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1011Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1071Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1461Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding1491Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding1521Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding1961Iron-sulfur 3 (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
O21241 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 1FB45AF558F469E2

FASTA69177,718
        10         20         30         40         50         60 
MVNVFVDGLS VEVKKGATIL QACAQVGIEI PRFCYHERLS IAGNCRMCLV EVEKSPKPVA 

        70         80         90        100        110        120 
SCAMPVMDNM KIFTNTPLVK KAREGVLEFL LVNHPLDCPI CDQGGECDLQ DLTMVYGSDR 

       130        140        150        160        170        180 
GRFHEYKRGV EDKNIGPLVK TVMTRCIHCT RCVRFATEVA GVPDLGTVGR GRDTEISTYI 

       190        200        210        220        230        240 
QKVFNSELSG NVIDLCPVGA LTSKPYAFTA RSWELQSTES IDVSDAIGSN IRIDVRGSEI 

       250        260        270        280        290        300 
MRILPRLNED VNEEWISDKA RFCYDGLKRQ RLNNPIIKEN GQYKTVTWEK AFNFILKNLQ 

       310        320        330        340        350        360 
EIQNSNRIVG VVGNLMDVES ILLFKELLNK LGSSKIYLES STPILQLNDD EKEDQILNNA 

       370        380        390        400        410        420 
DFRNNYISNT PLAKIEESDL CLLIGTNIRL EAPLLNTRIR KRYLQGNYSV YSVGPTNNLT 

       430        440        450        460        470        480 
YNTENLGNDI STLLEISEGR HPFCKKLMKS KKPLIIIGTH VLQRTDGTSI IELVKTLFKY 

       490        500        510        520        530        540 
TQIKTSNWNG FNILHTSASS VGALDLGIGS TKRYSEKISN SKIEKHFIYL LGADEIRIEN 

       550        560        570        580        590        600 
SKEHFIVYQG HHGDYGANIA DVILPGSAYT EKTATYVNVE GRVQNTKSAF YAPGNAREDW 

       610        620        630        640        650        660 
KIIRALSEVL NKKLPYDSFE DIHTRFMSIA PHLLKVNAIE KNKIVIENSL PFKGLIKNIG 

       670        680        690 
FKPLFNNFYL TNAICRSSQT MAKCSSIYKL N 

« Hide

References

[1]"An ancestral mitochondrial DNA resembling a eubacterial genome in miniature."
Lang B.F., Burger G., O'Kelly C.J., Cedergren R., Golding G.B., Lemieux C., Sankoff D., Turmel M., Gray M.W.
Nature 387:493-497(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 50394.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF007261 Genomic DNA. Translation: AAD11868.1.
PIRS78135.
RefSeqNP_044753.1. NC_001823.1.

3D structure databases

ProteinModelPortalO21241.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID801135.

Family and domain databases

Gene3D3.10.20.30. 1 hit.
InterProIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
IPR015405. NuoG_C.
[Graphical view]
PfamPF09326. DUF1982. 1 hit.
PF00111. Fer2. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SMARTSM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SUPFAMSSF54292. SSF54292. 1 hit.
TIGRFAMsTIGR01973. NuoG. 1 hit.
PROSITEPS51085. 2FE2S_FER_2. 1 hit.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00641. COMPLEX1_75K_1. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNDUS1_RECAM
AccessionPrimary (citable) accession number: O21241
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: February 19, 2014
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families