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O21241

- NDUS1_RECAM

UniProt

O21241 - NDUS1_RECAM

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Protein
NADH-ubiquinone oxidoreductase 75 kDa subunit
Gene
NAD11
Organism
Reclinomonas americana
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity.

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Binds 1 2Fe-2S cluster per subunit By similarity.
Binds 2 4Fe-4S clusters per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi34 – 341Iron-sulfur 1 (2Fe-2S) By similarity
Metal bindingi45 – 451Iron-sulfur 1 (2Fe-2S) By similarity
Metal bindingi48 – 481Iron-sulfur 1 (2Fe-2S) By similarity
Metal bindingi62 – 621Iron-sulfur 1 (2Fe-2S) By similarity
Metal bindingi94 – 941Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity
Metal bindingi98 – 981Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi101 – 1011Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi107 – 1071Iron-sulfur 2 (4Fe-4S) By similarity
Metal bindingi146 – 1461Iron-sulfur 3 (4Fe-4S) By similarity
Metal bindingi149 – 1491Iron-sulfur 3 (4Fe-4S) By similarity
Metal bindingi152 – 1521Iron-sulfur 3 (4Fe-4S) By similarity
Metal bindingi196 – 1961Iron-sulfur 3 (4Fe-4S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC
  4. electron carrier activity Source: InterPro
  5. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. ATP synthesis coupled electron transport Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-1631-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 75 kDa subunit (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-75kD
Short name:
CI-75kD
NADH dehydrogenase subunit 11
Gene namesi
Name:NAD11
Encoded oniMitochondrion
OrganismiReclinomonas americana
Taxonomic identifieri48483 [NCBI]
Taxonomic lineageiEukaryotaJakobidaHistionidaeReclinomonas

Subcellular locationi

Mitochondrion inner membrane By similarity
Note: Matrix and cytoplasmic side of the mitochondrial inner membrane By similarity.

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB-SubCell
  2. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 691691NADH-ubiquinone oxidoreductase 75 kDa subunit
PRO_0000118537Add
BLAST

Interactioni

Subunit structurei

Complex I is composed of about 30 different subunits By similarity.

Structurei

3D structure databases

ProteinModelPortaliO21241.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 78782Fe-2S ferredoxin-type
Add
BLAST
Domaini215 – 271574Fe-4S Mo/W bis-MGD-type
Add
BLAST

Sequence similaritiesi

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
IPR015405. NuoG_C.
[Graphical view]
PfamiPF09326. DUF1982. 1 hit.
PF00111. Fer2. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SMARTiSM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SUPFAMiSSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR01973. NuoG. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00641. COMPLEX1_75K_1. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O21241-1 [UniParc]FASTAAdd to Basket

« Hide

MVNVFVDGLS VEVKKGATIL QACAQVGIEI PRFCYHERLS IAGNCRMCLV    50
EVEKSPKPVA SCAMPVMDNM KIFTNTPLVK KAREGVLEFL LVNHPLDCPI 100
CDQGGECDLQ DLTMVYGSDR GRFHEYKRGV EDKNIGPLVK TVMTRCIHCT 150
RCVRFATEVA GVPDLGTVGR GRDTEISTYI QKVFNSELSG NVIDLCPVGA 200
LTSKPYAFTA RSWELQSTES IDVSDAIGSN IRIDVRGSEI MRILPRLNED 250
VNEEWISDKA RFCYDGLKRQ RLNNPIIKEN GQYKTVTWEK AFNFILKNLQ 300
EIQNSNRIVG VVGNLMDVES ILLFKELLNK LGSSKIYLES STPILQLNDD 350
EKEDQILNNA DFRNNYISNT PLAKIEESDL CLLIGTNIRL EAPLLNTRIR 400
KRYLQGNYSV YSVGPTNNLT YNTENLGNDI STLLEISEGR HPFCKKLMKS 450
KKPLIIIGTH VLQRTDGTSI IELVKTLFKY TQIKTSNWNG FNILHTSASS 500
VGALDLGIGS TKRYSEKISN SKIEKHFIYL LGADEIRIEN SKEHFIVYQG 550
HHGDYGANIA DVILPGSAYT EKTATYVNVE GRVQNTKSAF YAPGNAREDW 600
KIIRALSEVL NKKLPYDSFE DIHTRFMSIA PHLLKVNAIE KNKIVIENSL 650
PFKGLIKNIG FKPLFNNFYL TNAICRSSQT MAKCSSIYKL N 691
Length:691
Mass (Da):77,718
Last modified:January 1, 1998 - v1
Checksum:i1FB45AF558F469E2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF007261 Genomic DNA. Translation: AAD11868.1.
PIRiS78135.
RefSeqiNP_044753.1. NC_001823.1.

Genome annotation databases

GeneIDi801135.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF007261 Genomic DNA. Translation: AAD11868.1 .
PIRi S78135.
RefSeqi NP_044753.1. NC_001823.1.

3D structure databases

ProteinModelPortali O21241.
ModBasei Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 801135.

Enzyme and pathway databases

BioCyci RETL1328306-WGS:GSTH-1631-MONOMER.

Family and domain databases

Gene3Di 3.10.20.30. 1 hit.
InterProi IPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
IPR015405. NuoG_C.
[Graphical view ]
Pfami PF09326. DUF1982. 1 hit.
PF00111. Fer2. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view ]
SMARTi SM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view ]
SUPFAMi SSF54292. SSF54292. 1 hit.
TIGRFAMsi TIGR01973. NuoG. 1 hit.
PROSITEi PS51085. 2FE2S_FER_2. 1 hit.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00641. COMPLEX1_75K_1. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "An ancestral mitochondrial DNA resembling a eubacterial genome in miniature."
    Lang B.F., Burger G., O'Kelly C.J., Cedergren R., Golding G.B., Lemieux C., Sankoff D., Turmel M., Gray M.W.
    Nature 387:493-497(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 50394.

Entry informationi

Entry nameiNDUS1_RECAM
AccessioniPrimary (citable) accession number: O21241
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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