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Protein

NADH-ubiquinone oxidoreductase 75 kDa subunit

Gene

NAD11

Organism
Reclinomonas americana
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi34Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi45Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi48Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi62Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi94Iron-sulfur 2 (4Fe-4S); via pros nitrogenBy similarity1
Metal bindingi98Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi101Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi107Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi146Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi149Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi152Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi196Iron-sulfur 3 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 75 kDa subunit (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-75kD
Short name:
CI-75kD
NADH dehydrogenase subunit 11
Gene namesi
Name:NAD11
Encoded oniMitochondrion
OrganismiReclinomonas americana
Taxonomic identifieri48483 [NCBI]
Taxonomic lineageiEukaryotaJakobidaHistionidaeReclinomonas

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001185371 – 691NADH-ubiquinone oxidoreductase 75 kDa subunitAdd BLAST691

Interactioni

Subunit structurei

Complex I is composed of about 30 different subunits.By similarity

Structurei

3D structure databases

ProteinModelPortaliO21241.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 782Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST78
Domaini215 – 2714Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST57

Sequence similaritiesi

Belongs to the complex I 75 kDa subunit family.Curated
Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

Family and domain databases

CDDicd00207. fer2. 1 hit.
Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
IPR015405. NuoG_C.
[Graphical view]
PfamiPF00384. Molybdopterin. 1 hit.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
PF09326. NADH_dhqG_C. 1 hit.
[Graphical view]
SMARTiSM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SUPFAMiSSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR01973. NuoG. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00641. COMPLEX1_75K_1. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O21241-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNVFVDGLS VEVKKGATIL QACAQVGIEI PRFCYHERLS IAGNCRMCLV
60 70 80 90 100
EVEKSPKPVA SCAMPVMDNM KIFTNTPLVK KAREGVLEFL LVNHPLDCPI
110 120 130 140 150
CDQGGECDLQ DLTMVYGSDR GRFHEYKRGV EDKNIGPLVK TVMTRCIHCT
160 170 180 190 200
RCVRFATEVA GVPDLGTVGR GRDTEISTYI QKVFNSELSG NVIDLCPVGA
210 220 230 240 250
LTSKPYAFTA RSWELQSTES IDVSDAIGSN IRIDVRGSEI MRILPRLNED
260 270 280 290 300
VNEEWISDKA RFCYDGLKRQ RLNNPIIKEN GQYKTVTWEK AFNFILKNLQ
310 320 330 340 350
EIQNSNRIVG VVGNLMDVES ILLFKELLNK LGSSKIYLES STPILQLNDD
360 370 380 390 400
EKEDQILNNA DFRNNYISNT PLAKIEESDL CLLIGTNIRL EAPLLNTRIR
410 420 430 440 450
KRYLQGNYSV YSVGPTNNLT YNTENLGNDI STLLEISEGR HPFCKKLMKS
460 470 480 490 500
KKPLIIIGTH VLQRTDGTSI IELVKTLFKY TQIKTSNWNG FNILHTSASS
510 520 530 540 550
VGALDLGIGS TKRYSEKISN SKIEKHFIYL LGADEIRIEN SKEHFIVYQG
560 570 580 590 600
HHGDYGANIA DVILPGSAYT EKTATYVNVE GRVQNTKSAF YAPGNAREDW
610 620 630 640 650
KIIRALSEVL NKKLPYDSFE DIHTRFMSIA PHLLKVNAIE KNKIVIENSL
660 670 680 690
PFKGLIKNIG FKPLFNNFYL TNAICRSSQT MAKCSSIYKL N
Length:691
Mass (Da):77,718
Last modified:January 1, 1998 - v1
Checksum:i1FB45AF558F469E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007261 Genomic DNA. Translation: AAD11868.1.
PIRiS78135.
RefSeqiNP_044753.1. NC_001823.1.

Genome annotation databases

GeneIDi801135.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007261 Genomic DNA. Translation: AAD11868.1.
PIRiS78135.
RefSeqiNP_044753.1. NC_001823.1.

3D structure databases

ProteinModelPortaliO21241.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi801135.

Family and domain databases

CDDicd00207. fer2. 1 hit.
Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
IPR015405. NuoG_C.
[Graphical view]
PfamiPF00384. Molybdopterin. 1 hit.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
PF09326. NADH_dhqG_C. 1 hit.
[Graphical view]
SMARTiSM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SUPFAMiSSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR01973. NuoG. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00641. COMPLEX1_75K_1. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNDUS1_RECAM
AccessioniPrimary (citable) accession number: O21241
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 2, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.