Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O21241

- NDUS1_RECAM

UniProt

O21241 - NDUS1_RECAM

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

NADH-ubiquinone oxidoreductase 75 kDa subunit

Gene

NAD11

Organism
Reclinomonas americana
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
NADH + acceptor = NAD+ + reduced acceptor.

Cofactori

Protein has several cofactor binding sites:
  • [2Fe-2S] clusterBy similarityNote: Binds 1 [2Fe-2S] cluster per subunit.By similarity
  • [4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi34 – 341Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi45 – 451Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi48 – 481Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi62 – 621Iron-sulfur 1 (2Fe-2S)By similarity
Metal bindingi94 – 941Iron-sulfur 2 (4Fe-4S); via pros nitrogenBy similarity
Metal bindingi98 – 981Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi101 – 1011Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi107 – 1071Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi146 – 1461Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi149 – 1491Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi152 – 1521Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi196 – 1961Iron-sulfur 3 (4Fe-4S)By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. electron carrier activity Source: InterPro
  4. metal ion binding Source: UniProtKB-KW
  5. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

GO - Biological processi

  1. ATP synthesis coupled electron transport Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-1631-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase 75 kDa subunit (EC:1.6.5.3, EC:1.6.99.3)
Alternative name(s):
Complex I-75kD
Short name:
CI-75kD
NADH dehydrogenase subunit 11
Gene namesi
Name:NAD11
Encoded oniMitochondrion
OrganismiReclinomonas americana
Taxonomic identifieri48483 [NCBI]
Taxonomic lineageiEukaryotaJakobidaHistionidaeReclinomonas

Subcellular locationi

Mitochondrion inner membrane By similarity
Note: Matrix and cytoplasmic side of the mitochondrial inner membrane.By similarity

GO - Cellular componenti

  1. mitochondrial inner membrane Source: UniProtKB-KW
  2. respiratory chain Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 691691NADH-ubiquinone oxidoreductase 75 kDa subunitPRO_0000118537Add
BLAST

Interactioni

Subunit structurei

Complex I is composed of about 30 different subunits.By similarity

Structurei

3D structure databases

ProteinModelPortaliO21241.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 78782Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
BLAST
Domaini215 – 271574Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the complex I 75 kDa subunit family.Curated
Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di3.10.20.30. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
IPR015405. NuoG_C.
[Graphical view]
PfamiPF09326. DUF1982. 1 hit.
PF00111. Fer2. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SMARTiSM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SUPFAMiSSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR01973. NuoG. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00641. COMPLEX1_75K_1. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O21241-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVNVFVDGLS VEVKKGATIL QACAQVGIEI PRFCYHERLS IAGNCRMCLV
60 70 80 90 100
EVEKSPKPVA SCAMPVMDNM KIFTNTPLVK KAREGVLEFL LVNHPLDCPI
110 120 130 140 150
CDQGGECDLQ DLTMVYGSDR GRFHEYKRGV EDKNIGPLVK TVMTRCIHCT
160 170 180 190 200
RCVRFATEVA GVPDLGTVGR GRDTEISTYI QKVFNSELSG NVIDLCPVGA
210 220 230 240 250
LTSKPYAFTA RSWELQSTES IDVSDAIGSN IRIDVRGSEI MRILPRLNED
260 270 280 290 300
VNEEWISDKA RFCYDGLKRQ RLNNPIIKEN GQYKTVTWEK AFNFILKNLQ
310 320 330 340 350
EIQNSNRIVG VVGNLMDVES ILLFKELLNK LGSSKIYLES STPILQLNDD
360 370 380 390 400
EKEDQILNNA DFRNNYISNT PLAKIEESDL CLLIGTNIRL EAPLLNTRIR
410 420 430 440 450
KRYLQGNYSV YSVGPTNNLT YNTENLGNDI STLLEISEGR HPFCKKLMKS
460 470 480 490 500
KKPLIIIGTH VLQRTDGTSI IELVKTLFKY TQIKTSNWNG FNILHTSASS
510 520 530 540 550
VGALDLGIGS TKRYSEKISN SKIEKHFIYL LGADEIRIEN SKEHFIVYQG
560 570 580 590 600
HHGDYGANIA DVILPGSAYT EKTATYVNVE GRVQNTKSAF YAPGNAREDW
610 620 630 640 650
KIIRALSEVL NKKLPYDSFE DIHTRFMSIA PHLLKVNAIE KNKIVIENSL
660 670 680 690
PFKGLIKNIG FKPLFNNFYL TNAICRSSQT MAKCSSIYKL N
Length:691
Mass (Da):77,718
Last modified:January 1, 1998 - v1
Checksum:i1FB45AF558F469E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007261 Genomic DNA. Translation: AAD11868.1.
PIRiS78135.
RefSeqiNP_044753.1. NC_001823.1.

Genome annotation databases

GeneIDi801135.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007261 Genomic DNA. Translation: AAD11868.1 .
PIRi S78135.
RefSeqi NP_044753.1. NC_001823.1.

3D structure databases

ProteinModelPortali O21241.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 801135.

Enzyme and pathway databases

BioCyci RETL1328306-WGS:GSTH-1631-MONOMER.

Family and domain databases

Gene3Di 3.10.20.30. 1 hit.
InterProi IPR001041. 2Fe-2S_ferredoxin-type.
IPR012675. Beta-grasp_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
IPR010228. NADH_UbQ_OxRdtase_Gsu.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
IPR015405. NuoG_C.
[Graphical view ]
Pfami PF09326. DUF1982. 1 hit.
PF00111. Fer2. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view ]
SMARTi SM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view ]
SUPFAMi SSF54292. SSF54292. 1 hit.
TIGRFAMsi TIGR01973. NuoG. 1 hit.
PROSITEi PS51085. 2FE2S_FER_2. 1 hit.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00641. COMPLEX1_75K_1. 1 hit.
PS00642. COMPLEX1_75K_2. 1 hit.
PS00643. COMPLEX1_75K_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "An ancestral mitochondrial DNA resembling a eubacterial genome in miniature."
    Lang B.F., Burger G., O'Kelly C.J., Cedergren R., Golding G.B., Lemieux C., Sankoff D., Turmel M., Gray M.W.
    Nature 387:493-497(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 50394.

Entry informationi

Entry nameiNDUS1_RECAM
AccessioniPrimary (citable) accession number: O21241
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 26, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3