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O21241

- NDUS1_RECAM

UniProt

O21241 - NDUS1_RECAM

Protein

NADH-ubiquinone oxidoreductase 75 kDa subunit

Gene

NAD11

Organism
Reclinomonas americana
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 95 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone By similarity. This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized By similarity.By similarity

    Catalytic activityi

    NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).
    NADH + acceptor = NAD+ + reduced acceptor.

    Cofactori

    Binds 1 2Fe-2S cluster per subunit.By similarity
    Binds 2 4Fe-4S clusters per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi34 – 341Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi45 – 451Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi48 – 481Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi62 – 621Iron-sulfur 1 (2Fe-2S)By similarity
    Metal bindingi94 – 941Iron-sulfur 2 (4Fe-4S); via pros nitrogenBy similarity
    Metal bindingi98 – 981Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi101 – 1011Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi107 – 1071Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi146 – 1461Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi149 – 1491Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi152 – 1521Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi196 – 1961Iron-sulfur 3 (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. electron carrier activity Source: InterPro
    4. metal ion binding Source: UniProtKB-KW
    5. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB-EC

    GO - Biological processi

    1. ATP synthesis coupled electron transport Source: InterPro

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Respiratory chain, Transport

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

    Enzyme and pathway databases

    BioCyciRETL1328306-WGS:GSTH-1631-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NADH-ubiquinone oxidoreductase 75 kDa subunit (EC:1.6.5.3, EC:1.6.99.3)
    Alternative name(s):
    Complex I-75kD
    Short name:
    CI-75kD
    NADH dehydrogenase subunit 11
    Gene namesi
    Name:NAD11
    Encoded oniMitochondrion
    OrganismiReclinomonas americana
    Taxonomic identifieri48483 [NCBI]
    Taxonomic lineageiEukaryotaJakobidaHistionidaeReclinomonas

    Subcellular locationi

    Mitochondrion inner membrane By similarity
    Note: Matrix and cytoplasmic side of the mitochondrial inner membrane.By similarity

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: UniProtKB-SubCell
    2. respiratory chain Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 691691NADH-ubiquinone oxidoreductase 75 kDa subunitPRO_0000118537Add
    BLAST

    Interactioni

    Subunit structurei

    Complex I is composed of about 30 different subunits.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO21241.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1 – 78782Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini215 – 271574Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the complex I 75 kDa subunit family.Curated
    Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di3.10.20.30. 1 hit.
    InterProiIPR001041. 2Fe-2S_ferredoxin-type.
    IPR012675. Beta-grasp_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
    IPR010228. NADH_UbQ_OxRdtase_Gsu.
    IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
    IPR015405. NuoG_C.
    [Graphical view]
    PfamiPF09326. DUF1982. 1 hit.
    PF00111. Fer2. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF10588. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view]
    SMARTiSM00929. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view]
    SUPFAMiSSF54292. SSF54292. 1 hit.
    TIGRFAMsiTIGR01973. NuoG. 1 hit.
    PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
    PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00641. COMPLEX1_75K_1. 1 hit.
    PS00642. COMPLEX1_75K_2. 1 hit.
    PS00643. COMPLEX1_75K_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O21241-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVNVFVDGLS VEVKKGATIL QACAQVGIEI PRFCYHERLS IAGNCRMCLV    50
    EVEKSPKPVA SCAMPVMDNM KIFTNTPLVK KAREGVLEFL LVNHPLDCPI 100
    CDQGGECDLQ DLTMVYGSDR GRFHEYKRGV EDKNIGPLVK TVMTRCIHCT 150
    RCVRFATEVA GVPDLGTVGR GRDTEISTYI QKVFNSELSG NVIDLCPVGA 200
    LTSKPYAFTA RSWELQSTES IDVSDAIGSN IRIDVRGSEI MRILPRLNED 250
    VNEEWISDKA RFCYDGLKRQ RLNNPIIKEN GQYKTVTWEK AFNFILKNLQ 300
    EIQNSNRIVG VVGNLMDVES ILLFKELLNK LGSSKIYLES STPILQLNDD 350
    EKEDQILNNA DFRNNYISNT PLAKIEESDL CLLIGTNIRL EAPLLNTRIR 400
    KRYLQGNYSV YSVGPTNNLT YNTENLGNDI STLLEISEGR HPFCKKLMKS 450
    KKPLIIIGTH VLQRTDGTSI IELVKTLFKY TQIKTSNWNG FNILHTSASS 500
    VGALDLGIGS TKRYSEKISN SKIEKHFIYL LGADEIRIEN SKEHFIVYQG 550
    HHGDYGANIA DVILPGSAYT EKTATYVNVE GRVQNTKSAF YAPGNAREDW 600
    KIIRALSEVL NKKLPYDSFE DIHTRFMSIA PHLLKVNAIE KNKIVIENSL 650
    PFKGLIKNIG FKPLFNNFYL TNAICRSSQT MAKCSSIYKL N 691
    Length:691
    Mass (Da):77,718
    Last modified:January 1, 1998 - v1
    Checksum:i1FB45AF558F469E2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF007261 Genomic DNA. Translation: AAD11868.1.
    PIRiS78135.
    RefSeqiNP_044753.1. NC_001823.1.

    Genome annotation databases

    GeneIDi801135.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF007261 Genomic DNA. Translation: AAD11868.1 .
    PIRi S78135.
    RefSeqi NP_044753.1. NC_001823.1.

    3D structure databases

    ProteinModelPortali O21241.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 801135.

    Enzyme and pathway databases

    BioCyci RETL1328306-WGS:GSTH-1631-MONOMER.

    Family and domain databases

    Gene3Di 3.10.20.30. 1 hit.
    InterProi IPR001041. 2Fe-2S_ferredoxin-type.
    IPR012675. Beta-grasp_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR000283. NADH_UbQ_OxRdtase_75kDa_su_CS.
    IPR010228. NADH_UbQ_OxRdtase_Gsu.
    IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
    IPR015405. NuoG_C.
    [Graphical view ]
    Pfami PF09326. DUF1982. 1 hit.
    PF00111. Fer2. 1 hit.
    PF00384. Molybdopterin. 1 hit.
    PF10588. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view ]
    SMARTi SM00929. NADH-G_4Fe-4S_3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54292. SSF54292. 1 hit.
    TIGRFAMsi TIGR01973. NuoG. 1 hit.
    PROSITEi PS51085. 2FE2S_FER_2. 1 hit.
    PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00641. COMPLEX1_75K_1. 1 hit.
    PS00642. COMPLEX1_75K_2. 1 hit.
    PS00643. COMPLEX1_75K_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "An ancestral mitochondrial DNA resembling a eubacterial genome in miniature."
      Lang B.F., Burger G., O'Kelly C.J., Cedergren R., Golding G.B., Lemieux C., Sankoff D., Turmel M., Gray M.W.
      Nature 387:493-497(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 50394.

    Entry informationi

    Entry nameiNDUS1_RECAM
    AccessioniPrimary (citable) accession number: O21241
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 95 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3