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Protein

NADH-ubiquinone oxidoreductase subunit 8

Gene

NAD8

Organism
Reclinomonas americana
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). May donate electrons to ubiquinone.By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi63 – 631Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi69 – 691Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi73 – 731Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi102 – 1021Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi105 – 1051Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi108 – 1081Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi112 – 1121Iron-sulfur 1 (4Fe-4S)By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase subunit 8 (EC:1.6.5.3)
Gene namesi
Name:NAD8
Encoded oniMitochondrion
OrganismiReclinomonas americana
Taxonomic identifieri48483 [NCBI]
Taxonomic lineageiEukaryotaJakobidaHistionidaeReclinomonas

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 162162NADH-ubiquinone oxidoreductase subunit 8PRO_0000118718Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO21233.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini54 – 83304Fe-4S ferredoxin-type 1Add
BLAST
Domaini93 – 122304Fe-4S ferredoxin-type 2Add
BLAST

Sequence similaritiesi

Belongs to the complex I 23 kDa subunit family.Curated
Contains 2 4Fe-4S ferredoxin-type domains.Curated

Keywords - Domaini

Repeat

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O21233-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIINKTAQT LFLTELVKGM SLTLDYFFRK KVTLNYPFEK GPLSPRFRGE
60 70 80 90 100
HALRRYQTGE ERCIACKLCE AICPAQAITI ESEPRIDGSR RTTRYDIDMT
110 120 130 140 150
KCIYCGFCQE ACPVDAIVEG PNFEFATETH EELLYDKEKL LQNGDRWETE
160
IAANLANEAL YR
Length:162
Mass (Da):18,662
Last modified:January 1, 1998 - v1
Checksum:i0254DC6C0E54A724
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007261 Genomic DNA. Translation: AAD11860.1.
PIRiS78127.
RefSeqiNP_044745.1. NC_001823.1.

Genome annotation databases

GeneIDi801137.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007261 Genomic DNA. Translation: AAD11860.1.
PIRiS78127.
RefSeqiNP_044745.1. NC_001823.1.

3D structure databases

ProteinModelPortaliO21233.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi801137.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "An ancestral mitochondrial DNA resembling a eubacterial genome in miniature."
    Lang B.F., Burger G., O'Kelly C.J., Cedergren R., Golding G.B., Lemieux C., Sankoff D., Turmel M., Gray M.W.
    Nature 387:493-497(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 50394.

Entry informationi

Entry nameiNDUS8_RECAM
AccessioniPrimary (citable) accession number: O21233
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: September 16, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.