Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NADH-ubiquinone oxidoreductase subunit 8

Gene

NAD8

Organism
Reclinomonas americana
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). May donate electrons to ubiquinone.By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi63Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi66Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi69Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi73Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi102Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi105Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi108Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi112Iron-sulfur 1 (4Fe-4S)By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase subunit 8 (EC:1.6.5.3)
Gene namesi
Name:NAD8
Encoded oniMitochondrion
OrganismiReclinomonas americana
Taxonomic identifieri48483 [NCBI]
Taxonomic lineageiEukaryotaJakobidaHistionidaeReclinomonas

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001187181 – 162NADH-ubiquinone oxidoreductase subunit 8Add BLAST162

Structurei

3D structure databases

ProteinModelPortaliO21233.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 834Fe-4S ferredoxin-type 1Add BLAST30
Domaini93 – 1224Fe-4S ferredoxin-type 2Add BLAST30

Sequence similaritiesi

Belongs to the complex I 23 kDa subunit family.Curated

Keywords - Domaini

Repeat

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI. 1 hit.
InterProiView protein in InterPro
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
PfamiView protein in Pfam
PF12838. Fer4_7. 1 hit.
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiView protein in PROSITE
PS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.

Sequencei

Sequence statusi: Complete.

O21233-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIINKTAQT LFLTELVKGM SLTLDYFFRK KVTLNYPFEK GPLSPRFRGE
60 70 80 90 100
HALRRYQTGE ERCIACKLCE AICPAQAITI ESEPRIDGSR RTTRYDIDMT
110 120 130 140 150
KCIYCGFCQE ACPVDAIVEG PNFEFATETH EELLYDKEKL LQNGDRWETE
160
IAANLANEAL YR
Length:162
Mass (Da):18,662
Last modified:January 1, 1998 - v1
Checksum:i0254DC6C0E54A724
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007261 Genomic DNA. Translation: AAD11860.1.
PIRiS78127.
RefSeqiNP_044745.1. NC_001823.1.

Genome annotation databases

GeneIDi801137.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF007261 Genomic DNA. Translation: AAD11860.1.
PIRiS78127.
RefSeqiNP_044745.1. NC_001823.1.

3D structure databases

ProteinModelPortaliO21233.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi801137.

Family and domain databases

HAMAPiMF_01351. NDH1_NuoI. 1 hit.
InterProiView protein in InterPro
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR010226. NADH_quinone_OxRdtase_chainI.
PfamiView protein in Pfam
PF12838. Fer4_7. 1 hit.
TIGRFAMsiTIGR01971. NuoI. 1 hit.
PROSITEiView protein in PROSITE
PS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiNDUS8_RECAM
AccessioniPrimary (citable) accession number: O21233
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: February 15, 2017
This is version 85 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.