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Protein

NADH-ubiquinone oxidoreductase chain 4

Gene

nad4

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity).By similarity

Catalytic activityi

NADH + ubiquinone + 5 H+(In) = NAD+ + ubiquinol + 4 H+(Out).

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Keywords - Ligandi

NAD, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
NADH-ubiquinone oxidoreductase chain 4 (EC:1.6.5.3)
Alternative name(s):
NADH dehydrogenase subunit 4
Gene namesi
Name:nad4
ORF Names:DDB_G0294048
Encoded oniMitochondrion
OrganismiDictyostelium discoideum (Slime mold)
Taxonomic identifieri44689 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
Proteomesi
  • UP000002195 Componenti: Mitochondrion

Organism-specific databases

dictyBaseiDDB_G0294048. nad4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2 – 2221HelicalSequence analysisAdd
BLAST
Transmembranei35 – 5521HelicalSequence analysisAdd
BLAST
Transmembranei89 – 10921HelicalSequence analysisAdd
BLAST
Transmembranei118 – 13821HelicalSequence analysisAdd
BLAST
Transmembranei140 – 16021HelicalSequence analysisAdd
BLAST
Transmembranei172 – 19221HelicalSequence analysisAdd
BLAST
Transmembranei218 – 23821HelicalSequence analysisAdd
BLAST
Transmembranei248 – 26821HelicalSequence analysisAdd
BLAST
Transmembranei282 – 30221HelicalSequence analysisAdd
BLAST
Transmembranei310 – 33021HelicalSequence analysisAdd
BLAST
Transmembranei338 – 35821HelicalSequence analysisAdd
BLAST
Transmembranei376 – 39621HelicalSequence analysisAdd
BLAST
Transmembranei415 – 43521HelicalSequence analysisAdd
BLAST
Transmembranei501 – 52121HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 540540NADH-ubiquinone oxidoreductase chain 4PRO_0000311825Add
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the complex I subunit 4 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiO21047.
KOiK03881.
OMAiLYSAYKF.
PhylomeDBiO21047.

Family and domain databases

InterProiIPR010227. NADH_Q_OxRdtase_chainM/4.
IPR003918. NADH_UbQ_OxRdtase.
IPR001750. ND/Mrp_mem.
[Graphical view]
PfamiPF00361. Proton_antipo_M. 1 hit.
[Graphical view]
PRINTSiPR01437. NUOXDRDTASE4.
TIGRFAMsiTIGR01972. NDH_I_M. 1 hit.

Sequencei

Sequence statusi: Complete.

O21047-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIIAISIMN VVIGIAILGV ILRKKIMPNQ KFQRIFILGV QGILIVLSGI
60 70 80 90 100
MLIGCEGTDI INRISPYINM VTVYSNNVNI NIGYSIDGIS AIFIFLTIIL
110 120 130 140 150
ILSCNLISIR VIKEKTEQKF QIMLLLTEIL IINFFAATDL VQLYIVYEAT
160 170 180 190 200
LIPMVIMIGV WGSRTEKKIA AFQILIYTLI GSIFMLMSIG ILYSTLGTTD
210 220 230 240 250
YIMIREYIDV LPENVRKLIF IGFFIGFAVK IPIAPLHLWL LRAHVEAPTA
260 270 280 290 300
GSVLLAGILL KLGGYGYIRY NIGLFPDLCE YYFPIIGGIC LISILYTGIA
310 320 330 340 350
TLTQLDVKRI VAYSSISHMN VIVLGLFSGV LQGIEGGIIL MIGHGVVSGG
360 370 380 390 400
LFLCIGVIYD RCKTRIVYAY NNLVHVMPIM AILFFLLVLG NIAFPITSNF
410 420 430 440 450
VGELLIFIGL IKKNIIIAFF SALSMIVTAI YSFWLYNRIF FVNEIIKREA
460 470 480 490 500
NEVISSKGQI VADMNALFLI EDVMKKKEER GIDDIGQPKE VKKEQLIYSD
510 520 530 540
VNIFEFTSIS LMVIMMIIIG MKPSVVEGFI AINCLELISK
Length:540
Mass (Da):60,234
Last modified:January 1, 1998 - v1
Checksum:iD6C54F38BA01742C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16579 Genomic DNA. Translation: BAA21118.1.
AB000109 Genomic DNA. Translation: BAA78051.1.
PIRiT43747.
RefSeqiNP_050069.1. NC_000895.1.

Genome annotation databases

GeneIDi2193891.
KEGGiddi:DidioMp02.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D16579 Genomic DNA. Translation: BAA21118.1.
AB000109 Genomic DNA. Translation: BAA78051.1.
PIRiT43747.
RefSeqiNP_050069.1. NC_000895.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi2193891.
KEGGiddi:DidioMp02.

Organism-specific databases

dictyBaseiDDB_G0294048. nad4.

Phylogenomic databases

InParanoidiO21047.
KOiK03881.
OMAiLYSAYKF.
PhylomeDBiO21047.

Miscellaneous databases

PROiO21047.

Family and domain databases

InterProiIPR010227. NADH_Q_OxRdtase_chainM/4.
IPR003918. NADH_UbQ_OxRdtase.
IPR001750. ND/Mrp_mem.
[Graphical view]
PfamiPF00361. Proton_antipo_M. 1 hit.
[Graphical view]
PRINTSiPR01437. NUOXDRDTASE4.
TIGRFAMsiTIGR01972. NDH_I_M. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Group-I introns in the cytochrome c oxidase genes of Dictyostelium discoideum: two related ORFs in one loop of a group-I intron, a cox1/2 hybrid gene and an unusually large cox3 gene."
    Ogawa S., Matsuo K., Angata K., Yanagisawa K., Tanaka Y.
    Curr. Genet. 31:80-88(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: AX3.
  2. "The mitochondrial DNA of Dictyostelium discoideum: complete sequence, gene content and genome organization."
    Ogawa S., Yoshino R., Angata K., Iwamoto M., Pi M., Kuroe K., Matsuo K., Morio T., Urushihara H., Yanagisawa K., Tanaka Y.
    Mol. Gen. Genet. 263:514-519(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AX3.

Entry informationi

Entry nameiNU4M_DICDI
AccessioniPrimary (citable) accession number: O21047
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: January 1, 1998
Last modified: November 11, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Dictyostelium discoideum
    Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.