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O21042 (COX1_DICDI) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 99. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytochrome c oxidase subunit 1+2

EC=1.9.3.1
Alternative name(s):
Cytochrome c oxidase polypeptide I+II
Gene names
Name:cox1/2
Synonyms:cox1, coxI, Ddmco
ORF Names:DDB_G0294088
Encoded onMitochondrion
OrganismDictyostelium discoideum (Slime mold) [Reference proteome]
Taxonomic identifier44689 [NCBI]
Taxonomic lineageEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium

Protein attributes

Sequence length764 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B By similarity.

Catalytic activity

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Pathway

Energy metabolism; oxidative phosphorylation.

Subcellular location

Mitochondrion inner membrane; Multi-pass membrane protein.

Sequence similarities

In the N-terminal section; belongs to the heme-copper respiratory oxidase family.

In the C-terminal section; belongs to the cytochrome c oxidase subunit 2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 764764Cytochrome c oxidase subunit 1+2
PRO_0000312383

Regions

Transmembrane36 – 5621Helical; Potential
Transmembrane82 – 10221Helical; Potential
Transmembrane121 – 14121Helical; Potential
Transmembrane164 – 18421Helical; Potential
Transmembrane202 – 22221Helical; Potential
Transmembrane253 – 27321Helical; Potential
Transmembrane285 – 30521Helical; Potential
Transmembrane329 – 34921Helical; Potential
Transmembrane356 – 37621Helical; Potential
Transmembrane390 – 41021Helical; Potential
Transmembrane438 – 45821Helical; Potential
Transmembrane477 – 49721Helical; Potential
Transmembrane545 – 56521Helical; Potential
Transmembrane594 – 61421Helical; Potential
Region1 – 485485COX1
Region486 – 764279COX2

Sites

Metal binding801Iron (heme A axial ligand) By similarity
Metal binding2591Copper B By similarity
Metal binding2631Copper B By similarity
Metal binding3081Copper B By similarity
Metal binding3091Copper B By similarity
Metal binding3941Iron (heme A3 axial ligand) By similarity
Metal binding3961Iron (heme A axial ligand) By similarity
Metal binding6991Copper A By similarity
Metal binding7341Copper A By similarity
Metal binding7381Copper A By similarity
Metal binding7421Copper A By similarity

Amino acid modifications

Cross-link259 ↔ 2631'-histidyl-3'-tyrosine (His-Tyr) By similarity

Experimental info

Sequence conflict83 – 897IMIFFVV → MKVFMNC in AAB42021. Ref.3
Sequence conflict1041I → II in AAB42021. Ref.3
Sequence conflict1981S → P in AAB42021. Ref.3
Sequence conflict2591H → D in CAA65139. Ref.5

Sequences

Sequence LengthMass (Da)Tools
O21042 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: BF363A039A0BA912

FASTA76485,501
        10         20         30         40         50         60 
MKILEIYDKQ IAEKEGNIFI FISKWIISVD HKNIGTMYTN FSILAGIVGT LLSLVIRMEL 

        70         80         90        100        110        120 
STGNMLDGDG QQYNVIVTAH GLIMIFFVVM PAMLGGFANW FIPIMVGSPD VAFPRLNNIS 

       130        140        150        160        170        180 
LWLIIVSFFL LLTSSCVGIG VGTGWTVYPP LSTMEYHPGH AVDVGILSLH IAGASSLLGA 

       190        200        210        220        230        240 
INFLTTVFNM KIAGLSWSKV SLFVWSILIT AVLLVLSLPV LAGGLTMLIT DRNFETTFFD 

       250        260        270        280        290        300 
PIGGGDPILY QHLFWFFGHP EVYILILPGF GLVSIILSKY SNKGIFGVKG MISAMSAIGF 

       310        320        330        340        350        360 
LGFLVWAHHM YTVGLDVDTR AYFTAATMII AIPTGIKIFS WLATLWGGVI KITTPMLFVI 

       370        380        390        400        410        420 
GFLVLFTIGG LTGVVLANGG LDISLHDTYY VVAHFHYVLS MGAIFAIFAG YYYYYSIMNS 

       430        440        450        460        470        480 
TRLFGVVRYN EQLGRIHFWT MFIGVNVTFF PMHFLGLAGM PRRIGDYPDA YIGWNLIASY 

       490        500        510        520        530        540 
GSLITAFGLL FFVVNIFTPY IRRSVNIKNG AIILMGLDFA RDWQIGFQDP ATPIMEGIID 

       550        560        570        580        590        600 
LHNYIFFYLI VVAVFIGWVM GRILWRFSYK WSYPTIGDIE IFKNFTAYNQ IIHGTVIEIV 

       610        620        630        640        650        660 
WTLIPTVILY LIAIPSFTLL YAMDEIINPT VTIKIIGHQW YWSYEYGDNA SNLIEFDSYM 

       670        680        690        700        710        720 
VYERDLAEGQ LRLLEVDNAM VVPVKTHIRL IITSGDVLHS WAIPSFGIKV DAVPGRLNQI 

       730        740        750        760 
GLYVKREGTF YGQCSELCGV DHGFMPIKVQ AVKLGEYFSK LNEK 

« Hide

References

« Hide 'large scale' references
[1]"Group-I introns in the cytochrome c oxidase genes of Dictyostelium discoideum: two related ORFs in one loop of a group-I intron, a cox1/2 hybrid gene and an unusually large cox3 gene."
Ogawa S., Matsuo K., Angata K., Yanagisawa K., Tanaka Y.
Curr. Genet. 31:80-88(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: AX3.
[2]"The mitochondrial DNA of Dictyostelium discoideum: complete sequence, gene content and genome organization."
Ogawa S., Yoshino R., Angata K., Iwamoto M., Pi M., Kuroe K., Matsuo K., Morio T., Urushihara H., Yanagisawa K., Tanaka Y.
Mol. Gen. Genet. 263:514-519(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AX3.
[3]"Dictyostelium discoideum Ddmco gene sequence."
Mueller-Taubenberger A.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 83-205.
Strain: AX3.
[4]"Subunits I and II of Dictyostelium cytochrome c oxidase are specified by a single open reading frame transcribed into a large polycistronic RNA."
Pellizzari R., Anjard C., Bisson R.
Biochim. Biophys. Acta 1320:1-7(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 259-764.
Strain: AX3.
[5]Anjard C., Reymond C.D.
Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 259-341.
Strain: AX2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D50297 Genomic DNA. Translation: BAA21123.1.
AB000109 Genomic DNA. Translation: BAA78055.1.
U87391 mRNA. Translation: AAB42021.1.
X81884 Genomic DNA. Translation: CAA57467.1.
X95896 Genomic DNA. Translation: CAA65139.1.
PIRT43751.
RefSeqNP_050073.1. NC_000895.1.

3D structure databases

ProteinModelPortalO21042.
SMRO21042. Positions 20-494.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING44689.DidioMp06.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID2193894.
KEGGddi:DidioMp06.

Organism-specific databases

dictyBaseDDB_G0294088. cox1/2.

Phylogenomic databases

eggNOGCOG1622.
KOK02256.
OMAMISAMSA.
PhylomeDBO21042.

Enzyme and pathway databases

UniPathwayUPA00705.

Family and domain databases

Gene3D1.10.287.90. 1 hit.
1.20.210.10. 1 hit.
2.60.40.420. 1 hit.
InterProIPR001505. Copper_CuA.
IPR008972. Cupredoxin.
IPR000883. Cyt_c_Oxase_su1.
IPR023615. Cyt_c_Oxase_su1_BS.
IPR023616. Cyt_c_Oxase_su1_dom.
IPR014222. Cyt_c_oxidase_su2.
IPR002429. Cyt_c_oxidase_su2_C.
IPR011759. Cyt_c_oxidase_su2_TM_dom.
[Graphical view]
PANTHERPTHR10422. PTHR10422. 1 hit.
PfamPF00115. COX1. 1 hit.
PF00116. COX2. 1 hit.
PF02790. COX2_TM. 1 hit.
[Graphical view]
PRINTSPR01165. CYCOXIDASEI.
SUPFAMSSF49503. SSF49503. 1 hit.
SSF81442. SSF81442. 1 hit.
SSF81464. SSF81464. 1 hit.
TIGRFAMsTIGR02866. CoxB. 1 hit.
PROSITEPS50855. COX1. 1 hit.
PS00077. COX1_CUB. 1 hit.
PS00078. COX2. 1 hit.
PS50857. COX2_CUA. 1 hit.
PS50999. COX2_TM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROO21042.

Entry information

Entry nameCOX1_DICDI
AccessionPrimary (citable) accession number: O21042
Secondary accession number(s): P92625, Q23893, Q7GET3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 4, 2007
Last sequence update: January 1, 1998
Last modified: June 11, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Dictyostelium discoideum

Dictyostelium discoideum: entries, gene names and cross-references to dictyBase