Reviewed, UniProtKB/Swiss-Prot O19929 (ILVB_CYACA)

Names and origin

Protein names

Recommended name:
    Acetolactate synthase large subunit
      Short name=AHAS
    EC=2.2.1.6
Alternative name(s):
    Acetohydroxy-acid synthase large subunit
      Short name=ALS

Gene names

Name:

ilvB

Encoded on

Plastid; Chloroplast

Organism

Cyanidium caldarium

Taxonomic identifier

2771 [NCBI]

Taxonomic lineage

Eukaryota › Rhodophyta › Bangiophyceae › Cyanidiales › Cyanidiaceae › Cyanidium

Protein attributes

Sequence length	585 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 pyruvate = 2-acetolactate + CO2.
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4. Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.
Subunit structure	Dimer of large and small chains By similarity.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the TPP enzyme family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Cellular component	Chloroplast Plastid
Ligand	FAD Flavoprotein Magnesium Metal-binding Thiamine pyrophosphate
Molecular function	Transferase
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro acetolactate synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW thiamin pyrophosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 585	585	Acetolactate synthase large subunit		PRO_0000090809
Regions
	Nucleotide binding	272 – 293	22	FAD By similarity
	Nucleotide binding	315 – 334	20	FAD By similarity
	Region	407 – 486	80	Thiamine pyrophosphate binding
Sites
	Metal binding	457	1	Magnesium By similarity
	Metal binding	484	1	Magnesium By similarity
	Binding site	60	1	Thiamine pyrophosphate By similarity
	Binding site	162	1	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	O19929-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 17D95558C6F76011	FASTA	585	65,123
	10 20 30 40 50 60 MNYKKNSLIK TGAFALIDML VKHKVKNIFG YPGGAILPIY DELYHWEKKK LIKHYLVRHE 70 80 90 100 110 120 QSAAHAADAY ARATNEVGVC LATSGPGATN LVTGIATAQM DSVPIIAITG QVSRAFIGTD 130 140 150 160 170 180 AFQEVDIFGI TLPIVKHSFV VRDPRDISTI VSEAFYISKH GRPGAVLIDV PKDVGLEEFN 190 200 210 220 230 240 YHDYDSIRDH KPITKYRPIY GPSIRQIEKF KKMLLESKQP ILYVGGGAVM SRAQHEIEEL 250 260 270 280 290 300 ASFIKIPVTT TLMGKGSFNE YNPLYLGMLG MHGTAYANFA VSECDLLIAL GARFDDRVTG 310 320 330 340 350 360 KLDEFACNAQ VIHVDIDPAE IGKNRIPQLA IISDIKIVLK ELLSSMKEGT NNMDKNQTQA 370 380 390 400 410 420 WLHRIHKWKK EYPLSIPHDS KLLYPQEVIN EISQIAQKAF FATDVGQHQM WAAQFLKVEQ 430 440 450 460 470 480 GKWLSSSGLG TMGYGLPAAI GAKIANPNDL IICITGDASF QMNLQELGTI AQYELDIKIF 490 500 510 520 530 540 IINNQWQGMV RQWQQAFYDQ RYAHSNMAKG QPDFVQLANS YGIRGIRVTT SKDLKSKIER 550 560 570 580 IISTPGPLLI DCIVATSENC YPMIAPGKSN SQMLGLTKQL KTITN

References

[1]	"The structure and gene repertoire of an ancient red algal plastid genome." Gloeckner G., Rosenthal A., Valentin K.-U. J. Mol. Evol. 51:382-390(2000) [PubMed: 11040290] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RK-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF022186 Genomic DNA. Translation: AAB82660.1.
PIR	T11997.
RefSeq	NP_045101.1.
3D structure databases
HSSP	HSSP built from PDB template 1JSC based on UniProtKB P07342.
ModBase	Search...
Genome annotation databases
GeneID	800227.
Enzyme and pathway databases
BRENDA	2.2.1.6. 145.
Family and domain databases
InterPro	IPR012846. Acetolactate_synth_lsu. IPR000399. TPP_bd_CS. IPR012001. TPP_bd_enzyme_N. IPR011766. TPP_enzyme_bd_C. IPR012000. TPP_enzyme_M. [Graphical view]
Pfam	PF02775. TPP_enzyme_C. 1 hit. PF00205. TPP_enzyme_M. 1 hit. PF02776. TPP_enzyme_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00118. acolac_lg. 1 hit.
PROSITE	PS00187. TPP_ENZYMES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVB_CYACA

Accession

Primary (citable) accession number: O19929

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)