Reviewed, UniProtKB/Swiss-Prot O19906 (GLTB_CYACA)

Names and origin

Protein names

Recommended name:
    Ferredoxin-dependent glutamate synthase
    EC=1.4.7.1
Alternative name(s):
    Fd-GOGAT

Gene names

Name:

gltB

Encoded on

Plastid; Chloroplast

Organism

Cyanidium caldarium

Taxonomic identifier

2771 [NCBI]

Taxonomic lineage

Eukaryota › Rhodophyta › Bangiophyceae › Cyanidiales › Cyanidiaceae › Cyanidium

Protein attributes

Sequence length	1549 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 L-glutamate + 2 oxidized ferredoxin = L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+.
Cofactor	Binds 1 3Fe-4S cluster. FAD. FMN.
Pathway	Amino-acid biosynthesis; L-glutamate biosynthesis via GLT pathway; L-glutamate from 2-oxoglutarate and L-glutamine (ferredoxin route): step 1/1. Energy metabolism; nitrogen metabolism.
Subunit structure	Monomer By similarity.
Subcellular location	Plastid › chloroplast stroma.
Sequence similarities	Belongs to the glutamate synthase family. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Glutamate biosynthesis
Cellular component	Chloroplast Plastid
Domain	Glutamine amidotransferase
Ligand	3Fe-4S FAD FMN Flavoprotein Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate synthase (ferredoxin) activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1549	1549	Ferredoxin-dependent glutamate synthase		PRO_0000170791
Regions
	Domain	37 – 435	399	Glutamine amidotransferase type-2
	Nucleotide binding	1116 – 1173	58	FMN By similarity
Sites
	Active site	37	1	For GATase activity By similarity
	Metal binding	1169	1	Iron-sulfur (3Fe-4S) By similarity
	Metal binding	1175	1	Iron-sulfur (3Fe-4S) By similarity
	Metal binding	1180	1	Iron-sulfur (3Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	O19906-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 6C0965E071453CB4	FASTA	1,549	172,012
	10 20 30 40 50 60 MISISKRTSI ARINLSEFHT ISKIDRYPWL NKEKDACGVG FIAHLDNKFG HKLMMNALEA 70 80 90 100 110 120 LATMEHRGAC SADEESGDGA GILFSIPWKF FVEWSLRYKQ FKINISQAAV AMLFLPCLSS 130 140 150 160 170 180 DIQVSKNIVE EIFQDEDFIV IGWREVPYVK EVLGPLALRN MPQIYQIVVQ SKRYQGRSLD 190 200 210 220 230 240 FHLYRVRRKI EKEITVRAYS WAKDFYFCSC SNHTIVYKGM VKSTSLGQFY QDLYNPDFEI 250 260 270 280 290 300 SFAVFHRRFS TNTMPRWPLA QPMRILGHNG EINTLLGNLK WMEARESSLN HPTLNEVASI 310 320 330 340 350 360 GPVVNVSNSD SANLDSVVEL FLHVGHSCPE ALMFLIPEAY ENNPKLKYHQ NLISFYEYCA 370 380 390 400 410 420 GFQEAWDGPA LIVFSDGHTV GASLDRNGLR PARYCVTEDN VLILASEGGV LNLDPSLIRL 430 440 450 460 470 480 KGRLGPGEMI VLDLQEKLLM SNLEIKNKIA SLRPYSDWIK QNRQVLIPTS FLTSTTLPLQ 490 500 510 520 530 540 EVFKRQTCFG YTSEDIELVI ENMAIQGKEP TFCMGDDTPL AVLSGKSHVL YDYFKQRFAQ 550 560 570 580 590 600 VTNPPIDSLR ESLVMSISSY LGSKTNSFEE SSEKILKIKT PILSENDLVL IKNSELLTET 610 620 630 640 650 660 LVTTFEAHFD SPQANGQSLF STINQLCKQA KNLIQAGTKI IILSDKVCFE SRTESYIPPL 670 680 690 700 710 720 LVVGSLHQYL IKQGVRQKVS LIVETGQCWS THHFACLLGY GASAVCPYLA LETVRHWWMS 730 740 750 760 770 780 ERTQNLMSKG KMPNLTLIEV QNNYCKSVER GLLKILSKMG ISLLTSYIGA QIFEILGLGK 790 800 810 820 830 840 EVVDLAFEGT VSRIGGLSFA DLAMETIDLC SAGFSKLNKK KLDNHGFVQY RPGGEYHLNN 850 860 870 880 890 900 PEMSKALHKA VRENNYTLYE AYKQLLANRP PTNIRDLLEF NFRSCSVPLE KVENIFEITK 910 920 930 940 950 960 RFCTGGMSLG ALSREAHETL SIAMNRIGGK SNSGEGGEDS LRFTVLTDVD ETGNSPSFPH 970 980 990 1000 1010 1020 LKGLKNGDSL SSAIKQIASG RFGVTPEYLV NAKQLEIKIS QGAKPGEGGQ LPGKKVSPYI 1030 1040 1050 1060 1070 1080 ATLRACKPGV TLISPPPHHD IYSIEDLAQL IFDLHQVNPE CKVSVKLVSE IGVGTIAVGV 1090 1100 1110 1120 1130 1140 AKAGAEIIQI SGHDGGTGAS PLSSIKHAGV PWELGLHEVH CLLVENNLRE KVILRVDGGL 1150 1160 1170 1180 1190 1200 RTGQDVVMAA LLGADEYGFG TIAMIAGGCI MARVCHTNSC PVGVATQKEE LRMRYPGVPE 1210 1220 1230 1240 1250 1260 NVVNYFIFLA EEIRVILSKL GFETLSQIIG RKDLINHNFD KKLCKTHCID TSIFFNIKTN 1270 1280 1290 1300 1310 1320 EYNFLEIPGG HSKKLKTSLL DYELLNSSDI LYAIDNHKTL EKHIKISNSD RSVGAKLAGR 1330 1340 1350 1360 1370 1380 LAKQYKNEGF RGSLILNFYG TAGQSFGSFN IKGVTLRLIG EANDYVGKSM SGGEIVIVPP 1390 1400 1410 1420 1430 1440 SEVAFDASEQ VILGNTCLYG ATGGFLFAYG AAGERFAVRN SNAFSVLEGV GDHACEYMTG 1450 1460 1470 1480 1490 1500 GRVVVLGKAG RNIAAGMTGG IAYFLDEYSN LPEKVNLDIV RIQRVVTNEA RKQLIQLIEK 1510 1520 1530 1540 HVLKTGSKKA VLILQQWEIF IHYFWQIVPP SESETSETNY FVENKVLAN

References

[1]	"The structure and gene repertoire of an ancient red algal plastid genome." Gloeckner G., Rosenthal A., Valentin K.-U. J. Mol. Evol. 51:382-390(2000) [PubMed: 11040290] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RK-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF022186 Genomic DNA. Translation: AAB82683.1.
PIR	T11974.
RefSeq	NP_045078.1.
3D structure databases
HSSP	HSSP built from PDB template 1OFD based on UniProtKB P55038.
ModBase	Search...
Genome annotation databases
GeneID	800212.
Enzyme and pathway databases
BRENDA	1.4.7.1. 145.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR000583. GATase_2. IPR017932. GATase_II. IPR002932. Glu_synth_centr_C. IPR006982. Glu_synth_centr_N. IPR002489. Glu_synthase_C. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 2 hits. G3DSA:2.160.20.60. Glu_synthase_C. 1 hit.
Pfam	PF00310. GATase_2. 1 hit. PF04898. Glu_syn_central. 1 hit. PF01645. Glu_synthase. 1 hit. PF01493. GXGXG. 1 hit. [Graphical view]
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLTB_CYACA

Accession

Primary (citable) accession number: O19906

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)