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Protein

Uroporphyrinogen-III C-methyltransferase

Gene

cobA

Organism
Cyanidium caldarium
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin.By similarity

Catalytic activityi

S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1.
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei17 – 171S-adenosyl-L-methionine; via carbonyl oxygenBy similarity
Binding sitei123 – 1231S-adenosyl-L-methionineBy similarity
Binding sitei177 – 1771S-adenosyl-L-methionine; via amide nitrogenBy similarity

GO - Molecular functioni

  1. precorrin-2 dehydrogenase activity Source: InterPro
  2. uroporphyrin-III C-methyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. cobalamin biosynthetic process Source: UniProtKB-UniPathway
  2. siroheme biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Cobalamin biosynthesis, Porphyrin biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00148; UER00211.
UPA00262; UER00211.

Names & Taxonomyi

Protein namesi
Recommended name:
Uroporphyrinogen-III C-methyltransferase (EC:2.1.1.107)
Short name:
Urogen III methylase
Alternative name(s):
SUMT
Uroporphyrinogen III methylase
Short name:
UROM
Gene namesi
Name:cobA
Encoded oniPlastid; Chloroplast
OrganismiCyanidium caldarium
Taxonomic identifieri2771 [NCBI]
Taxonomic lineageiEukaryotaRhodophytaBangiophyceaeCyanidialesCyanidiaceaeCyanidium

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 251251Uroporphyrinogen-III C-methyltransferasePRO_0000150376Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliO19889.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni93 – 953S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Belongs to the precorrin methyltransferase family.Curated

Family and domain databases

Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF00590. TP_methylase. 1 hit.
[Graphical view]
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O19889-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTHNKTIKKV YIVGAGPGDP ELLTLKAQRV LREADVVIYD ALVNPEILQH
60 70 80 90 100
CKPNAEIIKV GKRRNNHSFS QLQIASLLID RAKKGERVIR LKGGDPLIFG
110 120 130 140 150
RLGEEILELF SKNIEIEIIP GITSAFAVAA DMKFPLTHRQ LGSSITFLTG
160 170 180 190 200
EEFYEKTTNK LKWERIIWGA DTIIVYMILY NLPKIIKKFL SVGYSAEKPI
210 220 230 240 250
VLVQWASLKK KNFLIGTIGT IIHQVIESKF GPPSLAIIGE VIEISSIIQK

L
Length:251
Mass (Da):28,126
Last modified:December 31, 1997 - v1
Checksum:i86F8734B91F94A76
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF022186 Genomic DNA. Translation: AAB82700.1.
PIRiT11957.
RefSeqiNP_045061.1. NC_001840.1.

Genome annotation databases

GeneIDi800259.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF022186 Genomic DNA. Translation: AAB82700.1.
PIRiT11957.
RefSeqiNP_045061.1. NC_001840.1.

3D structure databases

ProteinModelPortaliO19889.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi800259.

Enzyme and pathway databases

UniPathwayiUPA00148; UER00211.
UPA00262; UER00211.

Family and domain databases

Gene3Di3.30.950.10. 1 hit.
3.40.1010.10. 1 hit.
InterProiIPR000878. 4pyrrol_Mease.
IPR014777. 4pyrrole_Mease_sub1.
IPR014776. 4pyrrole_Mease_sub2.
IPR006366. CobA/CysG_C.
IPR003043. Uropor_MeTrfase_CS.
[Graphical view]
PfamiPF00590. TP_methylase. 1 hit.
[Graphical view]
SUPFAMiSSF53790. SSF53790. 1 hit.
TIGRFAMsiTIGR01469. cobA_cysG_Cterm. 1 hit.
PROSITEiPS00839. SUMT_1. 1 hit.
PS00840. SUMT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The structure and gene repertoire of an ancient red algal plastid genome."
    Gloeckner G., Rosenthal A., Valentin K.-U.
    J. Mol. Evol. 51:382-390(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: RK-1.

Entry informationi

Entry nameiSUMT_CYACA
AccessioniPrimary (citable) accession number: O19889
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 19, 2001
Last sequence update: December 31, 1997
Last modified: January 6, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.