Reviewed, UniProtKB/Swiss-Prot O19886 (CARA_CYACA)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:

carA

Encoded on

Plastid; Chloroplast

Organism

Cyanidium caldarium

Taxonomic identifier

2771 [NCBI]

Taxonomic lineage

Eukaryota › Rhodophyta › Bangiophyceae › Cyanidiales › Cyanidiaceae › Cyanidium

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from HCO(3)(-): step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 1/6. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity.
Subcellular location	Plastid › chloroplast. HAMAP MF_01209
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 399	399	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112369
Regions
	Domain	213 – 399	187	Glutamine amidotransferase type-1
	Region	1 – 209	209	CPSase HAMAP MF_01209
Sites
	Active site	289	1	Nucleophile By similarity
	Active site	372	1	By similarity
	Active site	374	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O19886-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 3BFDBB74FFA9218A	FASTA	399	45,519
	10 20 30 40 50 60 MEKFKLLKLG KPATLLLEDG TVFRGKSFSN PQTVTGEIVF NTGMTGYQEI FTDPSYNEQL 70 80 90 100 110 120 VVLTYPEIGN TGTNLDDYES LKIQAKGIIL KNLSNAYSNF RAMRSLLSFL KRHKVVEIHD 130 140 150 160 170 180 IDTRALVLHI RNNKTMLAVI SNETYNLTFK EAKATIKYGK HTLNSNLASR VSTSLDYYQW 190 200 210 220 230 240 MQPLNRKLKF GYSIIRSFNS AINKKLHTSK KLRIIVLDLG VKFNILRYLK SFECEVIIVN 250 260 270 280 290 300 YKSTYQQVMK FHPDGIVISN GPGDPAKINR VVVRVNKFIN KKIPILGICL GHQILARCFE 310 320 330 340 350 360 AKTFKLKFGH RGLNHPVGIN KRMEITSQNH GFAVNFEFLK SNKFYANHLN LNDGTLAGIS 370 380 390 SQNFPLISVQ YHPEGSPGPH DSNYLFKYWV YIIYKSKSS

References

[1]	"The structure and gene repertoire of an ancient red algal plastid genome." Gloeckner G., Rosenthal A., Valentin K.-U. J. Mol. Evol. 51:382-390(2000) [PubMed: 11040290] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RK-1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF022186 Genomic DNA. Translation: AAB82703.1.
PIR	T11954.
RefSeq	NP_045058.1.
3D structure databases
HSSP	HSSP built from PDB template 1M6V based on UniProtKB P00907.
ModBase	Search...
Genome annotation databases
GeneID	800256.
Enzyme and pathway databases
BRENDA	6.3.5.5. 145.
Family and domain databases
HAMAP	MF_01209. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_CYACA

Accession

Primary (citable) accession number: O19886

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	January 1, 1998
Last modified:	June 16, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)