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O19116 (SFRP1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Secreted frizzled-related protein 1

Short name=sFRP-1
Alternative name(s):
Frizzled in aorta protein
Short name=FrzA protein
Gene names
Name:SFRP1
Synonyms:FRZA
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length308 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels By similarity. Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase By similarity. In kidney development, inhibits tubule formation and bud growth in metanephroi By similarity. Inhibits WNT1/WNT4-mediated TCF-dependent transcription.

Subunit structure

Interacts with WNT1, WNT2, WNT4, WNT8, MYOC and FRZD6 By similarity.

Subcellular location

Secreted. Note: Cell membrane or extracellular matrix-associated. Released by heparin-binding. Ref.1

Tissue specificity

Highest levels in aortic endothelium, heart, spleen and eye. Lower levels in lung, brain and kidney. Weak expression in liver, skeletal muscle and the medial layer of the aorta. In the cortical brain, localized to neurons and small blood vessels. In the retina, localized to the inner and outer nuclear layers with high expression in the neuronal cell bodies. In the heart, restricted to myocytes. In lung, highest expression found in the epithelium of terminal bronchioles. In kidney, localized to the epithelium of collecting ducts of the medulla and, in spleen, expression restricted to the red pulp in cells associated with the sinuses. Ref.1

Domain

The FZ domain is involved in binding with Wnt ligands By similarity.

Sequence similarities

Belongs to the secreted frizzled-related protein (sFRP) family.

Contains 1 FZ (frizzled) domain.

Contains 1 NTR domain.

Ontologies

Keywords
   Biological processDifferentiation
Wnt signaling pathway
   Cellular componentSecreted
   DomainSignal
   Molecular functionDevelopmental protein
   PTMDisulfide bond
Glycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processactin cytoskeleton organization

Inferred from direct assay PubMed 18156211. Source: MGI

brain development

Inferred from Biological aspect of Ancestor. Source: RefGenome

canonical Wnt signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

gonad development

Inferred from Biological aspect of Ancestor. Source: RefGenome

positive regulation of Rac GTPase activity

Inferred from mutant phenotype PubMed 18156211. Source: BHF-UCL

positive regulation of cell-matrix adhesion

Inferred from direct assay PubMed 18156211. Source: BHF-UCL

positive regulation of focal adhesion assembly

Inferred from direct assay PubMed 18156211. Source: BHF-UCL

positive regulation of stress fiber assembly

Inferred from direct assay PubMed 18156211. Source: BHF-UCL

regulation of angiogenesis

Inferred from mutant phenotype PubMed 18156211. Source: BHF-UCL

substrate adhesion-dependent cell spreading

Inferred from direct assay PubMed 18156211. Source: MGI

   Cellular_componentcytoplasm

Inferred from Biological aspect of Ancestor. Source: RefGenome

extracellular space

Inferred from direct assay PubMed 18156211. Source: BHF-UCL

plasma membrane

Inferred from mutant phenotype PubMed 18156211. Source: BHF-UCL

   Molecular_functionPDZ domain binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Wnt-activated receptor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Wnt-protein binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

frizzled binding

Inferred from physical interaction PubMed 18156211. Source: BHF-UCL

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2525 Potential
Chain26 – 308283Secreted frizzled-related protein 1
PRO_0000032537

Regions

Domain47 – 163117FZ
Domain180 – 300121NTR

Amino acid modifications

Glycosylation1671N-linked (GlcNAc...) By similarity
Disulfide bond52 ↔ 115 By similarity
Disulfide bond62 ↔ 108 By similarity
Disulfide bond99 ↔ 134 By similarity
Disulfide bond123 ↔ 160 By similarity
Disulfide bond127 ↔ 151 By similarity
Disulfide bond180 ↔ 250 By similarity
Disulfide bond183 ↔ 252 By similarity
Disulfide bond197 ↔ 300 By similarity

Sequences

Sequence LengthMass (Da)Tools
O19116 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 184D138B31123FEB

FASTA30834,763
        10         20         30         40         50         60 
MGGGRWAAAG ALLALAAGLL AAGSASEYDY VSFQSDIGAY QSGRFYTKPP QCVDIPADLR 

        70         80         90        100        110        120 
LCHNVGYKRM VLPNLLEHET MAEVKQQASS WVPLLNKNCH IGTQVFLCSL FAPVCLDRPI 

       130        140        150        160        170        180 
YPCRWLCEAV RDSCEPVMQF FGFYWPEMLK CDKFPEGDVC IAMTPPNATE ASKPQGTTVC 

       190        200        210        220        230        240 
PPCDNELKSE AIIEHLCASE FALRMKIKEV KKENGDKKIV PKKKKPLKLG PIKKKELKKL 

       250        260        270        280        290        300 
VLYLKNGADC PCHQLDNLSH HFLIMGRKVK SQYLLTAIHK WDKKNKEFKT FMKKMKNHEC 


PTFQSVFK 

« Hide

References

[1]"Identification and cloning of a secreted protein related to the cysteine-rich domain of frizzled: evidence for a role in endothelial cell growth control."
Duplaa C., Jaspard B., Moreau C., D'Amore P.A.
Circ. Res. 84:1433-1445(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Aortic endothelium.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U85945 mRNA. Translation: AAB67062.1.
RefSeqNP_776885.1. NM_174460.2.
UniGeneBt.5226.

3D structure databases

ProteinModelPortalO19116.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid159350. 2 interactions.
STRING9913.ENSBTAP00000039575.

Protein family/group databases

MEROPSI93.001.

Proteomic databases

PRIDEO19116.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID282068.
KEGGbta:282068.

Organism-specific databases

CTD6422.

Phylogenomic databases

eggNOGNOG240950.
HOGENOMHOG000231058.
HOVERGENHBG052928.
InParanoidO19116.
KOK02166.

Family and domain databases

Gene3D1.10.2000.10. 1 hit.
InterProIPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR026559. SFRP1.
IPR008993. TIMP-like_OB-fold.
[Graphical view]
PANTHERPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF46. PTHR11309:SF46. 1 hit.
PfamPF01392. Fz. 1 hit.
PF01759. NTR. 1 hit.
[Graphical view]
SMARTSM00643. C345C. 1 hit.
SM00063. FRI. 1 hit.
[Graphical view]
SUPFAMSSF50242. SSF50242. 1 hit.
SSF63501. SSF63501. 1 hit.
PROSITEPS50038. FZ. 1 hit.
PS50189. NTR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20805919.

Entry information

Entry nameSFRP1_BOVIN
AccessionPrimary (citable) accession number: O19116
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: January 1, 1998
Last modified: July 9, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families