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Protein

Secreted frizzled-related protein 1

Gene

SFRP1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription.By similarity

GO - Molecular functioni

  1. frizzled binding Source: BHF-UCL

GO - Biological processi

  1. actin cytoskeleton organization Source: MGI
  2. multicellular organismal development Source: UniProtKB-KW
  3. positive regulation of cell-matrix adhesion Source: BHF-UCL
  4. positive regulation of focal adhesion assembly Source: BHF-UCL
  5. positive regulation of Rac GTPase activity Source: BHF-UCL
  6. positive regulation of stress fiber assembly Source: BHF-UCL
  7. regulation of angiogenesis Source: BHF-UCL
  8. substrate adhesion-dependent cell spreading Source: MGI
  9. Wnt signaling pathway Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Wnt signaling pathway

Protein family/group databases

MEROPSiI93.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Secreted frizzled-related protein 1
Short name:
sFRP-1
Alternative name(s):
Frizzled in aorta protein
Short name:
FrzA protein
Gene namesi
Name:SFRP1
Synonyms:FRZA
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

Secreted 1 Publication
Note: Cell membrane or extracellular matrix-associated. Released by heparin-binding.

GO - Cellular componenti

  1. extracellular space Source: BHF-UCL
  2. plasma membrane Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence AnalysisAdd
BLAST
Chaini26 – 308283Secreted frizzled-related protein 1PRO_0000032537Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi52 ↔ 115By similarity
Disulfide bondi62 ↔ 108By similarity
Disulfide bondi99 ↔ 134By similarity
Disulfide bondi123 ↔ 160By similarity
Disulfide bondi127 ↔ 151By similarity
Glycosylationi167 – 1671N-linked (GlcNAc...)By similarity
Disulfide bondi180 ↔ 250By similarity
Disulfide bondi183 ↔ 252By similarity
Disulfide bondi197 ↔ 300By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiO19116.

Expressioni

Tissue specificityi

Highest levels in aortic endothelium, heart, spleen and eye. Lower levels in lung, brain and kidney. Weak expression in liver, skeletal muscle and the medial layer of the aorta. In the cortical brain, localized to neurons and small blood vessels. In the retina, localized to the inner and outer nuclear layers with high expression in the neuronal cell bodies. In the heart, restricted to myocytes. In lung, highest expression found in the epithelium of terminal bronchioles. In kidney, localized to the epithelium of collecting ducts of the medulla and, in spleen, expression restricted to the red pulp in cells associated with the sinuses.1 Publication

Interactioni

Subunit structurei

Interacts with WNT1, WNT2, WNT4, WNT8, MYOC and FRZD6.By similarity

Protein-protein interaction databases

BioGridi159350. 2 interactions.
STRINGi9913.ENSBTAP00000039575.

Structurei

3D structure databases

ProteinModelPortaliO19116.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini47 – 163117FZPROSITE-ProRule annotationAdd
BLAST
Domaini180 – 300121NTRPROSITE-ProRule annotationAdd
BLAST

Domaini

The FZ domain is involved in binding with Wnt ligands.By similarity

Sequence similaritiesi

Contains 1 FZ (frizzled) domain.PROSITE-ProRule annotation
Contains 1 NTR domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG240950.
HOGENOMiHOG000231058.
HOVERGENiHBG052928.
InParanoidiO19116.
KOiK02166.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiIPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR026559. SFRP1.
IPR008993. TIMP-like_OB-fold.
[Graphical view]
PANTHERiPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF46. PTHR11309:SF46. 1 hit.
PfamiPF01392. Fz. 1 hit.
PF01759. NTR. 1 hit.
[Graphical view]
SMARTiSM00643. C345C. 1 hit.
SM00063. FRI. 1 hit.
[Graphical view]
SUPFAMiSSF50242. SSF50242. 1 hit.
SSF63501. SSF63501. 1 hit.
PROSITEiPS50038. FZ. 1 hit.
PS50189. NTR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O19116-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGGGRWAAAG ALLALAAGLL AAGSASEYDY VSFQSDIGAY QSGRFYTKPP
60 70 80 90 100
QCVDIPADLR LCHNVGYKRM VLPNLLEHET MAEVKQQASS WVPLLNKNCH
110 120 130 140 150
IGTQVFLCSL FAPVCLDRPI YPCRWLCEAV RDSCEPVMQF FGFYWPEMLK
160 170 180 190 200
CDKFPEGDVC IAMTPPNATE ASKPQGTTVC PPCDNELKSE AIIEHLCASE
210 220 230 240 250
FALRMKIKEV KKENGDKKIV PKKKKPLKLG PIKKKELKKL VLYLKNGADC
260 270 280 290 300
PCHQLDNLSH HFLIMGRKVK SQYLLTAIHK WDKKNKEFKT FMKKMKNHEC

PTFQSVFK
Length:308
Mass (Da):34,763
Last modified:January 1, 1998 - v1
Checksum:i184D138B31123FEB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85945 mRNA. Translation: AAB67062.1.
RefSeqiNP_776885.1. NM_174460.2.
UniGeneiBt.5226.

Genome annotation databases

GeneIDi282068.
KEGGibta:282068.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U85945 mRNA. Translation: AAB67062.1.
RefSeqiNP_776885.1. NM_174460.2.
UniGeneiBt.5226.

3D structure databases

ProteinModelPortaliO19116.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi159350. 2 interactions.
STRINGi9913.ENSBTAP00000039575.

Protein family/group databases

MEROPSiI93.001.

Proteomic databases

PRIDEiO19116.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi282068.
KEGGibta:282068.

Organism-specific databases

CTDi6422.

Phylogenomic databases

eggNOGiNOG240950.
HOGENOMiHOG000231058.
HOVERGENiHBG052928.
InParanoidiO19116.
KOiK02166.

Miscellaneous databases

NextBioi20805919.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiIPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR026559. SFRP1.
IPR008993. TIMP-like_OB-fold.
[Graphical view]
PANTHERiPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF46. PTHR11309:SF46. 1 hit.
PfamiPF01392. Fz. 1 hit.
PF01759. NTR. 1 hit.
[Graphical view]
SMARTiSM00643. C345C. 1 hit.
SM00063. FRI. 1 hit.
[Graphical view]
SUPFAMiSSF50242. SSF50242. 1 hit.
SSF63501. SSF63501. 1 hit.
PROSITEiPS50038. FZ. 1 hit.
PS50189. NTR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Identification and cloning of a secreted protein related to the cysteine-rich domain of frizzled: evidence for a role in endothelial cell growth control."
    Duplaa C., Jaspard B., Moreau C., D'Amore P.A.
    Circ. Res. 84:1433-1445(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Aortic endothelium.

Entry informationi

Entry nameiSFRP1_BOVIN
AccessioniPrimary (citable) accession number: O19116
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: January 1, 1998
Last modified: February 4, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.