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O19114

- NOS2_RABIT

UniProt

O19114 - NOS2_RABIT

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Protein

Nitric oxide synthase, inducible

Gene
NOS2
Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 By similarity.

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Heme group By similarity.
Binds 1 FAD By similarity.
Binds 1 FMN By similarity.
Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.

Enzyme regulationi

Not stimulated by calcium/calmodulin By similarity.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi‹1 – 25›25FMN By similarityAdd
BLAST
Nucleotide bindingi138 – 14912FAD By similarityAdd
BLAST
Nucleotide bindingi274 – 28411FAD By similarityAdd
BLAST
Nucleotide bindingi349 – 36719NADP By similarityAdd
BLAST
Nucleotide bindingi447 – 46216NADP By similarityAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. nitric-oxide synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. peptidyl-cysteine S-nitrosylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase, inducible (EC:1.14.13.39)
Alternative name(s):
Inducible NO synthase
Short name:
Inducible NOS
Short name:
iNOS
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2
Gene namesi
Name:NOS2
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
ProteomesiUP000001811: Unplaced

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – ›496›496Nitric oxide synthase, induciblePRO_0000170936Add
BLAST

Interactioni

Subunit structurei

Homodimer. Binds SLC9A3R1 By similarity.

Structurei

3D structure databases

ProteinModelPortaliO19114.
SMRiO19114. Positions 74-496.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini101 – 341241FAD-binding FR-typeAdd
BLAST

Sequence similaritiesi

Belongs to the NOS family.

Family and domain databases

Gene3Di1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProiIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PfamiPF00667. FAD_binding_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSiPR00371. FPNCR.
SUPFAMiSSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEiPS51384. FAD_FR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

O19114-1 [UniParc]FASTAAdd to Basket

« Hide

MYPHFCAFVM TSTRNCLTWA ASQLAPTGEG DELSGQEDAF RSWAVQTFRA    50
ACETFDVRSK HCIQIPKRYT SNATWEPEQY KLTQSPEPLD LNKALSSIHA 100
KNLFTMRLRS LQNLHSEKSS RTTLLVQLTF EGSRGPSYLP GEHLGIFPGN 150
QTGLVQGILE RVVDCSSPDQ TVCLEVHDES GSYWVKDKRL PPCSLRQALT 200
YFLDITTPPT QLQLHKLARF ATEETHRQRL EALCQPSEYN DWKFSNNPTF 250
LEVLEEFPSL RVPAAFLLSQ LPILKPRYYS ISSSQDHTPS EVHLTVAVVT 300
YRTRDGQGPL HHGVCSTWIN NLKPEDPVPC FVRSVSGFQL PEDPSQPCIL 350
IGPGTGIAPF RSFWQQRLHD SQHRGLKGGR MTLVFGCRHP EEDHLYQEEM 400
QEMVRKGVLF QVHTGYSRLP GKPKVYVQDI LQKELADEVF SVLHGEQGHL 450
YVCGDVRMAR DVATTLKKLV AAKLNLSEEQ VEDYFFQLKY HEDIFG 496
Length:496
Mass (Da):56,397
Last modified:January 1, 1998 - v1
Checksum:iA0A1D303C57F0EEC
GO

Non-terminal residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Non-terminal residuei496 – 4961

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U85094 mRNA. Translation: AAB65618.1.
UniGeneiOcu.2556.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U85094 mRNA. Translation: AAB65618.1 .
UniGenei Ocu.2556.

3D structure databases

ProteinModelPortali O19114.
SMRi O19114. Positions 74-496.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.20.990.10. 1 hit.
3.40.50.360. 1 hit.
InterProi IPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR029039. Flavoprotein-like.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view ]
Pfami PF00667. FAD_binding_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view ]
PRINTSi PR00371. FPNCR.
SUPFAMi SSF52218. SSF52218. 1 hit.
SSF63380. SSF63380. 1 hit.
PROSITEi PS51384. FAD_FR. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Rabbit iducible nitric oxide synthase gene-NOS II."
    Broner C.W., Eledath F.M.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: New Zealand white.
    Tissue: Heart.

Entry informationi

Entry nameiNOS2_RABIT
AccessioniPrimary (citable) accession number: O19114
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: June 11, 2014
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3