Reviewed, UniProtKB/Swiss-Prot O19114 (NOS2_RABIT)

Names and origin

Protein names

Recommended name:
    Nitric oxide synthase, inducible
    EC=1.14.13.39
Alternative name(s):
    Inducible NO synthase
      Short name=Inducible NOS
      Short name=iNOS
    NOS type II

Gene names

Name:

NOS2

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	496 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body.
Catalytic activity	L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+.
Cofactor	Heme group By similarity. Binds 1 FAD By similarity. Binds 1 FMN By similarity. Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.
Enzyme regulation	Not stimulated by calcium/calmodulin By similarity.
Subunit structure	Homodimer. Binds SLC9A3R1 By similarity.
Sequence similarities	Belongs to the NOS family. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Ligand	Calmodulin-binding FAD FMN Heme Iron Metal-binding NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitric-oxide synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›496	›496	Nitric oxide synthase, inducible		PRO_0000170936
Regions
	Domain	101 – 341	241	FAD-binding FR-type
	Nucleotide binding	‹1 – 25	›25	FMN By similarity
	Nucleotide binding	138 – 149	12	FAD By similarity
	Nucleotide binding	274 – 284	11	FAD By similarity
	Nucleotide binding	349 – 367	19	NADP By similarity
	Nucleotide binding	447 – 462	16	NADP By similarity
Amino acid modifications
	Modified residue	85	1	Phosphoserine By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	496	1

Sequences

	Sequence		Length	Mass (Da)
	O19114-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: A0A1D303C57F0EEC	FASTA	496	56,397
	10 20 30 40 50 60 MYPHFCAFVM TSTRNCLTWA ASQLAPTGEG DELSGQEDAF RSWAVQTFRA ACETFDVRSK 70 80 90 100 110 120 HCIQIPKRYT SNATWEPEQY KLTQSPEPLD LNKALSSIHA KNLFTMRLRS LQNLHSEKSS 130 140 150 160 170 180 RTTLLVQLTF EGSRGPSYLP GEHLGIFPGN QTGLVQGILE RVVDCSSPDQ TVCLEVHDES 190 200 210 220 230 240 GSYWVKDKRL PPCSLRQALT YFLDITTPPT QLQLHKLARF ATEETHRQRL EALCQPSEYN 250 260 270 280 290 300 DWKFSNNPTF LEVLEEFPSL RVPAAFLLSQ LPILKPRYYS ISSSQDHTPS EVHLTVAVVT 310 320 330 340 350 360 YRTRDGQGPL HHGVCSTWIN NLKPEDPVPC FVRSVSGFQL PEDPSQPCIL IGPGTGIAPF 370 380 390 400 410 420 RSFWQQRLHD SQHRGLKGGR MTLVFGCRHP EEDHLYQEEM QEMVRKGVLF QVHTGYSRLP 430 440 450 460 470 480 GKPKVYVQDI LQKELADEVF SVLHGEQGHL YVCGDVRMAR DVATTLKKLV AAKLNLSEEQ 490 VEDYFFQLKY HEDIFG

References

[1]	"Rabbit iducible nitric oxide synthase gene-NOS II." Broner C.W., Eledath F.M. Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: New Zealand white. Tissue: Heart.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U85094 mRNA. Translation: AAB65618.1.
UniGene	Ocu.2556
3D structure databases
HSSP	HSSP built from PDB template 1F20 based on UniProtKB P29476.
ModBase	Search...
Phylogenomic databases
HOVERGEN	O19114.
Enzyme and pathway databases
BRENDA	1.14.13.39. 255.
Family and domain databases
InterPro	IPR003097. FAD-binding_1. IPR017927. Fd_Rdtase_FAD-bd. IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase. IPR004030. NO_synthase_oxygenase_reg. IPR001433. OxRdtase_FAD/NAD_bd. [Graphical view]
Pfam	PF00667. FAD_binding_1. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00371. FPNCR.
PROSITE	PS51384. FAD_FR. 1 hit. PS60001. NOS. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NOS2_RABIT

Accession

Primary (citable) accession number: O19114

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 1, 1998
Last modified:	May 5, 2009
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)