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O19114 (NOS2_RABIT) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitric oxide synthase, inducible

EC=1.14.13.39
Alternative name(s):
Inducible NO synthase
Short name=Inducible NOS
Short name=iNOS
NOS type II
Peptidyl-cysteine S-nitrosylase NOS2
Gene names
Name:NOS2
OrganismOryctolagus cuniculus (Rabbit) [Reference proteome]
Taxonomic identifier9986 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus

Protein attributes

Sequence length496 AA.
Sequence statusFragment.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2 By similarity.

Catalytic activity

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor

Heme group By similarity.

Binds 1 FAD By similarity.

Binds 1 FMN By similarity.

Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.

Enzyme regulation

Not stimulated by calcium/calmodulin By similarity.

Subunit structure

Homodimer. Binds SLC9A3R1 By similarity.

Sequence similarities

Belongs to the NOS family.

Contains 1 FAD-binding FR-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›496›496Nitric oxide synthase, inducible
PRO_0000170936

Regions

Domain101 – 341241FAD-binding FR-type
Nucleotide binding‹1 – 25›25FMN By similarity
Nucleotide binding138 – 14912FAD By similarity
Nucleotide binding274 – 28411FAD By similarity
Nucleotide binding349 – 36719NADP By similarity
Nucleotide binding447 – 46216NADP By similarity

Experimental info

Non-terminal residue11
Non-terminal residue4961

Sequences

Sequence LengthMass (Da)Tools
O19114 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: A0A1D303C57F0EEC

FASTA49656,397
        10         20         30         40         50         60 
MYPHFCAFVM TSTRNCLTWA ASQLAPTGEG DELSGQEDAF RSWAVQTFRA ACETFDVRSK 

        70         80         90        100        110        120 
HCIQIPKRYT SNATWEPEQY KLTQSPEPLD LNKALSSIHA KNLFTMRLRS LQNLHSEKSS 

       130        140        150        160        170        180 
RTTLLVQLTF EGSRGPSYLP GEHLGIFPGN QTGLVQGILE RVVDCSSPDQ TVCLEVHDES 

       190        200        210        220        230        240 
GSYWVKDKRL PPCSLRQALT YFLDITTPPT QLQLHKLARF ATEETHRQRL EALCQPSEYN 

       250        260        270        280        290        300 
DWKFSNNPTF LEVLEEFPSL RVPAAFLLSQ LPILKPRYYS ISSSQDHTPS EVHLTVAVVT 

       310        320        330        340        350        360 
YRTRDGQGPL HHGVCSTWIN NLKPEDPVPC FVRSVSGFQL PEDPSQPCIL IGPGTGIAPF 

       370        380        390        400        410        420 
RSFWQQRLHD SQHRGLKGGR MTLVFGCRHP EEDHLYQEEM QEMVRKGVLF QVHTGYSRLP 

       430        440        450        460        470        480 
GKPKVYVQDI LQKELADEVF SVLHGEQGHL YVCGDVRMAR DVATTLKKLV AAKLNLSEEQ 

       490 
VEDYFFQLKY HEDIFG 

« Hide

References

[1]"Rabbit iducible nitric oxide synthase gene-NOS II."
Broner C.W., Eledath F.M.
Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: New Zealand white.
Tissue: Heart.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U85094 mRNA. Translation: AAB65618.1.
UniGeneOcu.2556.

3D structure databases

ProteinModelPortalO19114.
SMRO19114. Positions 74-496.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.20.990.10. 1 hit.
InterProIPR003097. FAD-binding_1.
IPR017927. Fd_Rdtase_FAD-bd.
IPR001709. Flavoprot_Pyr_Nucl_cyt_Rdtase.
IPR023173. NADPH_Cyt_P450_Rdtase_dom3.
IPR012144. NOS_euk.
IPR001433. OxRdtase_FAD/NAD-bd.
IPR017938. Riboflavin_synthase-like_b-brl.
[Graphical view]
PANTHERPTHR19384:SF5. PTHR19384:SF5. 1 hit.
PfamPF00667. FAD_binding_1. 1 hit.
PF00175. NAD_binding_1. 1 hit.
[Graphical view]
PRINTSPR00371. FPNCR.
SUPFAMSSF63380. SSF63380. 1 hit.
PROSITEPS51384. FAD_FR. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNOS2_RABIT
AccessionPrimary (citable) accession number: O19114
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: February 19, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families