O19113 (CTGF_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Connective tissue growth factor

Alternative name(s):
CCN family member 2

Gene names

Name:	CTGF
Synonyms:	CCN2

Organism

Sus scrofa (Pig) [Reference proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes By similarity. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells By similarity. Enhances fibroblast growth factor-induced DNA synthesis By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix By similarity. Secreted By similarity.
Sequence similarities	Belongs to the CCN family. Contains 1 CTCK (C-terminal cystine knot-like) domain. Contains 1 IGFBP N-terminal domain. Contains 1 TSP type-1 domain. Contains 1 VWFC domain.

Ontologies

Keywords
Biological process	Cell adhesion DNA synthesis
Cellular component	Extracellular matrix Secreted
Domain	Signal
Ligand	Heparin-binding
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell growth Inferred from electronic annotation. Source: InterPro
Cellular_component	proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	heparin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 349	323	Connective tissue growth factor		PRO_0000014404
Regions
	Domain	27 – 98	72	IGFBP N-terminal
	Domain	101 – 167	67	VWFC
	Domain	198 – 243	46	TSP type-1
	Domain	256 – 330	75	CTCK
	Region	247 – 349	103	Heparin-binding By similarity
Amino acid modifications
	Disulfide bond	256 ↔ 293		By similarity
	Disulfide bond	273 ↔ 307		By similarity
	Disulfide bond	284 ↔ 323		By similarity
	Disulfide bond	287 ↔ 325		By similarity
	Disulfide bond	292 ↔ 329		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O19113 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: BB510E2B2B52D4A0	FASTA	349	38,007
	10 20 30 40 50 60 MSATGLSPVR CAFVLLLALC SRPASGQDCS GQCQCAAGKR RACPAGVSLV LDGCGCCRLC 70 80 90 100 110 120 AKQLGELCTE RDPCDPHKGL FCDFGSPANR KIGVCTAKDG APCVFGGTVY RSGESFQSSC 130 140 150 160 170 180 KYQCTCLDGA VGCVPLCSMD VRLPSPDCPF PRRVKLPGKC CEEWVCDEPK DHTVVGPALA 190 200 210 220 230 240 AYRLEDTFGP DPTMMRANCL VQTTEWSACS KTCGMGISTR VTNDNAFCRL EKQSRLCMVR 250 260 270 280 290 300 PCEADLEENI KKGKKCIRTP KISKPVKFEL SGCTSVKTYR AKFCGVCTDG RCCTPHRTTT 310 320 330 340 LPVEFKCPDG EVMKKSMMFI KTCACHYNCP GDNDIFESLY YRKMYGDMA

References

[1]

"Purification and characterization of novel heparin-binding growth factors in uterine secretory fluids. Identification as heparin-regulated Mr 10,000 forms of connective tissue growth factor." Brigstock D.R., Steffen C.L., Kim G.Y., Vegunta R.K., Diehl J.R., Harding P.A. J. Biol. Chem. 272:20275-20282(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Uterus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U83916 mRNA. Translation: AAC48756.1.
UniGene	Ssc.70001.
3D structure databases
ProteinModelPortal	O19113.
ModBase	Search...
Proteomic databases
PRIDE	O19113.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	NOG72934.
HOGENOM	HOG000231462.
HOVERGEN	HBG000635.
OrthoDB	EOG44XJGZ.
Family and domain databases
InterPro	IPR006208. Cys_knot. IPR006207. Cys_knot_C. IPR000867. IGFBP-like. IPR012395. IGFBP_CNN. IPR017891. Insulin_GF-bd_Cys-rich_CS. IPR000884. Thrombospondin_1_rpt. IPR001007. VWF_C. [Graphical view]
Pfam	PF00007. Cys_knot. 1 hit. PF00219. IGFBP. 1 hit. PF00090. TSP_1. 1 hit. PF00093. VWC. 1 hit. [Graphical view]
PIRSF	PIRSF036495. IGFBP_rP_CNN. 1 hit.
SMART	SM00041. CT. 1 hit. SM00121. IB. 1 hit. SM00209. TSP1. 1 hit. SM00214. VWC. 1 hit. [Graphical view]
SUPFAM	SSF82895. TSP1. 1 hit.
PROSITE	PS01185. CTCK_1. 1 hit. PS01225. CTCK_2. 1 hit. PS00222. IGFBP_N_1. 1 hit. PS51323. IGFBP_N_2. 1 hit. PS50092. TSP1. 1 hit. PS01208. VWFC_1. 1 hit. PS50184. VWFC_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CTGF_PIG

Accession

Primary (citable) accession number: O19113

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	April 3, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program