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O19111

- KPCZ_RABIT

UniProt

O19111 - KPCZ_RABIT

Protein

Protein kinase C zeta type

Gene

PRKCZ

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Calcium- and diacylglycerol-independent serine/threonine-protein kinase that functions in phosphatidylinositol 3-kinase (PI3K) pathway and mitogen-activated protein (MAP) kinase cascade, and is involved in NF-kappa-B activation, mitogenic signaling, cell proliferation, cell polarity, inflammatory response and maintenance of long-term potentiation (LTP). Upon lipopolysaccharide (LPS) treatment in macrophages, or following mitogenic stimuli, functions downstream of PI3K to activate MAP2K1/MEK1-MAPK1/ERK2 signaling cascade independently of RAF1 activation. Required for insulin-dependent activation of AKT3, but may function as an adapter rather than a direct activator. Upon insulin treatment may act as a downstream effector of PI3K and contribute to the activation of translocation of the glucose transporter SLC2A4/GLUT4 and subsequent glucose transport in adipocytes. In EGF-induced cells, binds and activates MAP2K5/MEK5-MAPK7/ERK5 independently of its kinase activity and can activate JUN promoter through MEF2C. Through binding with SQSTM1/p62, functions in interleukin-1 signaling and activation of NF-kappa-B with the specific adapters RIPK1 and TRAF6. Participates in TNF-dependent transactivation of NF-kappa-B by phosphorylating and activating IKBKB kinase, which in turn leads to the degradation of NF-kappa-B inhibitors. In migrating astrocytes, forms a cytoplasmic complex with PARD6A and is recruited by CDC42 to function in the establishment of cell polarity along with the microtubule motor and dynein. In association with FEZ1, stimulates neuronal differentiation in PC12 cells. In the inflammatory response, is required for the T-helper 2 (Th2) differentiation process, including interleukin production, efficient activation of JAK1 and the subsequent phosphorylation and nuclear translocation of STAT6. May be involved in development of allergic airway inflammation (asthma), a process dependent on Th2 immune response. In the NF-kappa-B-mediated inflammatory response, can relieve SETD6-dependent repression of NF-kappa-B target genes by phosphorylating the RELA subunit at 'Ser-311'. Necessary and sufficient for LTP maintenance in hippocampal CA1 pyramidal cells. In vein endothelial cells treated with the oxidant peroxynitrite, phosphorylates STK11 leading to nuclear export of STK11, subsequent inhibition of PI3K/Akt signaling, and increased apoptosis By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Enzyme regulationi

    Atypical PKCs (PRKCI and PRKCZ) exhibit an elevated basal enzymatic activity (that may be due to the interaction with SMG1 or SQSTM1) and are not regulated by diacylglycerol, phosphatidylserine, phorbol esters or calcium ions. Two specific sites, Thr-409 (activation loop of the kinase domain) and Thr-559 (turn motif), need to be phosphorylated for its full activation. Phosphatidylinositol 3,4,5-trisphosphate might be a physiological activator By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei280 – 2801ATPPROSITE-ProRule annotation
    Active sitei375 – 3751Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri130 – 18051Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
    BLAST
    Nucleotide bindingi257 – 2659ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein kinase C activity Source: UniProtKB-EC
    3. protein serine/threonine kinase activity Source: UniProtKB
    4. zinc ion binding Source: InterPro

    GO - Biological processi

    1. establishment of cell polarity Source: UniProtKB
    2. inflammatory response Source: UniProtKB-KW
    3. intracellular signal transduction Source: InterPro
    4. long-term synaptic potentiation Source: UniProtKB
    5. positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
    6. positive regulation of excitatory postsynaptic membrane potential Source: UniProtKB
    7. positive regulation of insulin receptor signaling pathway Source: UniProtKB
    8. positive regulation of interleukin-10 secretion Source: UniProtKB
    9. positive regulation of interleukin-13 secretion Source: UniProtKB
    10. positive regulation of interleukin-4 production Source: UniProtKB
    11. positive regulation of interleukin-5 secretion Source: UniProtKB
    12. positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
    13. positive regulation of T-helper 2 cell cytokine production Source: UniProtKB
    14. positive regulation of T-helper 2 cell differentiation Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Inflammatory response

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BRENDAi2.7.11.13. 1749.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein kinase C zeta type (EC:2.7.11.13)
    Alternative name(s):
    nPKC-zeta
    Gene namesi
    Name:PRKCZ
    OrganismiOryctolagus cuniculus (Rabbit)
    Taxonomic identifieri9986 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
    ProteomesiUP000001811: Unplaced

    Subcellular locationi

    Cytoplasm By similarity. Endosome By similarity. Cell junction By similarity
    Note: In the retina, localizes in the terminals of the rod bipolar cells. Associated with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction By similarity.By similarity

    GO - Cellular componenti

    1. cell-cell junction Source: UniProtKB
    2. endosome Source: UniProtKB-SubCell
    3. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell junction, Cytoplasm, Endosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 591591Protein kinase C zeta typePRO_0000055703Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei409 – 4091Phosphothreonine; by PDPK1 and PI3KBy similarity
    Modified residuei559 – 5591PhosphothreonineBy similarity

    Post-translational modificationi

    CDH5 is required for its phosphorylation at Thr-409. Phosphorylated by protein kinase PDPK1; phosphorylation is inhibited by the apoptotic C-terminal cleavage product of PKN2. Phosphorylation at Thr-409 by PI3K activates the kinase By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Interacts with PARD6A, PARD6B and PARD6G. Part of a complex with PARD3, PARD6A or PARD6B or PARD6G and CDC42 or RAC1. Interacts with ADAP1/CENTA1. Interacts directly with SQSTM1. Forms a ternary complex with SQSTM1 and KCNAB2. Forms another ternary complex with SQSTM1 and GABRR3. Forms a complex with SQSTM1 and MAP2K5. Interacts (via the protein kinase domain) with WWC1. Forms a tripartite complex with WWC1 and DDR1, but predominantly in the absence of collagen. Component of the Par polarity complex, composed of at least phosphorylated PRKCZ, PARD3 and TIAM1. Interacts with PDPK1 (via N-terminal region) By similarity.By similarity

    Protein-protein interaction databases

    STRINGi9986.ENSOCUP00000006125.

    Structurei

    3D structure databases

    ProteinModelPortaliO19111.
    SMRiO19111. Positions 12-98, 246-583.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini15 – 9884OPRAdd
    BLAST
    Domaini251 – 517267Protein kinasePROSITE-ProRule annotationAdd
    BLAST
    Domaini518 – 58972AGC-kinase C-terminalAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni79 – 14567Interaction with SQSTM1By similarityAdd
    BLAST

    Domaini

    The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.By similarity
    The C1 domain does not bind the diacylglycerol (DAG).

    Sequence similaritiesi

    Contains 1 AGC-kinase C-terminal domain.Curated
    Contains 1 OPR domain.Curated
    Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri130 – 18051Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    eggNOGiCOG0515.
    HOVERGENiHBG108317.

    Family and domain databases

    InterProiIPR000961. AGC-kinase_C.
    IPR020454. DAG/PE-bd.
    IPR011009. Kinase-like_dom.
    IPR000270. OPR_PB1.
    IPR012233. PKC_zeta.
    IPR017892. Pkinase_C.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PANTHERiPTHR24357:SF60. PTHR24357:SF60. 1 hit.
    PfamiPF00130. C1_1. 1 hit.
    PF00564. PB1. 1 hit.
    PF00069. Pkinase. 1 hit.
    PF00433. Pkinase_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000554. PKC_zeta. 1 hit.
    PRINTSiPR00008. DAGPEDOMAIN.
    SMARTiSM00109. C1. 1 hit.
    SM00666. PB1. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS00479. ZF_DAG_PE_1. 1 hit.
    PS50081. ZF_DAG_PE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O19111-1 [UniParc]FASTAAdd to Basket

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    MPSRAGPKMD GSGGRVRLKA HYSGDIFITS VDAATTFEEL CEEVRDMCGL    50
    HQHHPLTLKW VDSEGDPRTV SSQMELGEAF RLAGQHRDDG LILHVFPSTP 100
    EQPGMPCPGE DKSIYRRGAR RWRKLYRANG HLFQAKRFNR RAYCGQCSER 150
    IWGLARQGYR CINCKLLVHK RCHGLVPLTC RRHMDSVMPS QEPPVADKSD 200
    DADLPSQETD GIAFISTRKQ DSGQEDAEDL KPVIDGVDGI KISQGLGLQD 250
    FDLIRVIGRG SYAKVLLVRL KKNGQVYAMK VVKKELVHDD EDIDWVQTEK 300
    HVFEQASGNP FLVGLHSCFQ TTSRLFLVIE YVNGGDLMFH MQRQRKLPEE 350
    HARFYAAEIC IALNFLHERG IIYRDLKLDN VLLDADGHIK LTDYGMCKEG 400
    LGPGDTTSTF CGTPNYIAPE ILRGEEYGFS VDWWALGVLM FEMMAGRSPF 450
    DIITDNPDMN TEDYLFQVIL EKPIRIPRFL SVKASHVLKG FLNKDPKERL 500
    GCRPQTGFSD IKSHAFFRSI DWDLLEKKQA LPPFQPQITD DYGLDNSDTQ 550
    FTSEPVQLTP DDEDVIKRID QSEFEGFEYI NPLLLSTEES V 591
    Length:591
    Mass (Da):67,095
    Last modified:January 1, 1998 - v1
    Checksum:i83D32A8744CADDB6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U78768 mRNA. Translation: AAB67317.1.
    RefSeqiNP_001076227.1. NM_001082758.1.
    UniGeneiOcu.2166.

    Genome annotation databases

    GeneIDi100009538.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U78768 mRNA. Translation: AAB67317.1 .
    RefSeqi NP_001076227.1. NM_001082758.1.
    UniGenei Ocu.2166.

    3D structure databases

    ProteinModelPortali O19111.
    SMRi O19111. Positions 12-98, 246-583.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9986.ENSOCUP00000006125.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100009538.

    Organism-specific databases

    CTDi 5590.

    Phylogenomic databases

    eggNOGi COG0515.
    HOVERGENi HBG108317.

    Enzyme and pathway databases

    BRENDAi 2.7.11.13. 1749.

    Family and domain databases

    InterProi IPR000961. AGC-kinase_C.
    IPR020454. DAG/PE-bd.
    IPR011009. Kinase-like_dom.
    IPR000270. OPR_PB1.
    IPR012233. PKC_zeta.
    IPR017892. Pkinase_C.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    PANTHERi PTHR24357:SF60. PTHR24357:SF60. 1 hit.
    Pfami PF00130. C1_1. 1 hit.
    PF00564. PB1. 1 hit.
    PF00069. Pkinase. 1 hit.
    PF00433. Pkinase_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000554. PKC_zeta. 1 hit.
    PRINTSi PR00008. DAGPEDOMAIN.
    SMARTi SM00109. C1. 1 hit.
    SM00666. PB1. 1 hit.
    SM00133. S_TK_X. 1 hit.
    SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS51285. AGC_KINASE_CTER. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS00479. ZF_DAG_PE_1. 1 hit.
    PS50081. ZF_DAG_PE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: New Zealand white.
      Tissue: Kidney.

    Entry informationi

    Entry nameiKPCZ_RABIT
    AccessioniPrimary (citable) accession number: O19111
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3