Reviewed, UniProtKB/Swiss-Prot O19111 (KPCZ_RABIT)

Names and origin

Protein names

Recommended name:
    Protein kinase C zeta type
    EC=2.7.11.13
Alternative name(s):
    nPKC-zeta

Gene names

Name:

PRKCZ

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates KCNAB2. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Phosphatidylinositol 3,4,5-trisphosphate might be a physiological activator. Two specific sites, Thr-409 (activation loop of the kinase domain) and Thr-559 (turn motif), need to be phosphorylated for its full activation.
Subunit structure	Interacts with PARD6A, PARD6B and PARD6G. Part of a complex with PARD3, PARD6A or PARD6B or PARD6G and CDC42 or RAC1. Interacts with ADAP1/CENTA1. Interacts directly with SQSTM1. Forms a ternary complex with SQSTM1 and KCNAB2. Forms another ternary complex with SQSTM1 and GABRR3. Forms a complex with SQSTM1 and MAP2K5 By similarity.
Subcellular location	Cytoplasm By similarity. Endosome By similarity. Note: In the retina, localizes in the terminals of the rod bipolar cells. Associated with endosomes By similarity.
Domain	The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B By similarity. The C1 domain does not bind the diacylglycerol (DAG).
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 OPR domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Endosome
Domain	Phorbol-ester binding Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	intracellular signaling cascade Inferred from electronic annotation. Source: InterPro protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	endosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW diacylglycerol binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase C activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 591	591	Protein kinase C zeta type		PRO_0000055703
Regions
	Domain	15 – 98	84	OPR
	Domain	251 – 517	267	Protein kinase
	Domain	518 – 589	72	AGC-kinase C-terminal
	Zinc finger	130 – 180	51	Phorbol-ester/DAG-type
	Nucleotide binding	257 – 265	9	ATP By similarity
	Region	79 – 145	67	Interaction with SQSTM1 By similarity
Sites
	Active site	375	1	Proton acceptor By similarity
	Binding site	280	1	ATP By similarity
Amino acid modifications
	Modified residue	222	1	Phosphoserine By similarity
	Modified residue	409	1	Phosphothreonine; by PDPK1 By similarity
	Modified residue	559	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O19111-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 83D32A8744CADDB6	FASTA	591	67,095
	10 20 30 40 50 60 MPSRAGPKMD GSGGRVRLKA HYSGDIFITS VDAATTFEEL CEEVRDMCGL HQHHPLTLKW 70 80 90 100 110 120 VDSEGDPRTV SSQMELGEAF RLAGQHRDDG LILHVFPSTP EQPGMPCPGE DKSIYRRGAR 130 140 150 160 170 180 RWRKLYRANG HLFQAKRFNR RAYCGQCSER IWGLARQGYR CINCKLLVHK RCHGLVPLTC 190 200 210 220 230 240 RRHMDSVMPS QEPPVADKSD DADLPSQETD GIAFISTRKQ DSGQEDAEDL KPVIDGVDGI 250 260 270 280 290 300 KISQGLGLQD FDLIRVIGRG SYAKVLLVRL KKNGQVYAMK VVKKELVHDD EDIDWVQTEK 310 320 330 340 350 360 HVFEQASGNP FLVGLHSCFQ TTSRLFLVIE YVNGGDLMFH MQRQRKLPEE HARFYAAEIC 370 380 390 400 410 420 IALNFLHERG IIYRDLKLDN VLLDADGHIK LTDYGMCKEG LGPGDTTSTF CGTPNYIAPE 430 440 450 460 470 480 ILRGEEYGFS VDWWALGVLM FEMMAGRSPF DIITDNPDMN TEDYLFQVIL EKPIRIPRFL 490 500 510 520 530 540 SVKASHVLKG FLNKDPKERL GCRPQTGFSD IKSHAFFRSI DWDLLEKKQA LPPFQPQITD 550 560 570 580 590 DYGLDNSDTQ FTSEPVQLTP DDEDVIKRID QSEFEGFEYI NPLLLSTEES V

References

[1]	"Intrarenal distribution of rabbit PKC zeta." Hao C.-M., Breyer R.M., Davis L.S., Breyer M.D. Kidney Int. 51:1831-1837(1997) [PubMed: 9186872] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: New Zealand white. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U78768 mRNA. Translation: AAB67317.1.
RefSeq	NP_001076227.1.
UniGene	Ocu.2166
3D structure databases
HSSP	HSSP built from PDB template 1GZK based on UniProtKB P31751.
ModBase	Search...
Genome annotation databases
GeneID	100009538.
Organism-specific databases
CTD	100009538.
Phylogenomic databases
HOVERGEN	O19111.
Enzyme and pathway databases
BRENDA	2.7.11.13. 255.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR020454. DAG/PE_bd. IPR000270. OPR_PB1. IPR015745. PKC. IPR012233. PKC_zeta. IPR017892. Pkinase_C. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
PANTHER	PTHR22985:SF86. PKC. 1 hit.
Pfam	PF00130. C1_1. 1 hit. PF00564. PB1. 1 hit. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
PIRSF	PIRSF000554. PKC_zeta. 1 hit.
PRINTS	PR00008. DAGPEDOMAIN.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00109. C1. 1 hit. SM00666. PB1. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 1 hit. PS50081. ZF_DAG_PE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPCZ_RABIT

Accession

Primary (citable) accession number: O19111

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	January 1, 1998
Last modified:	September 22, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)