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Protein

Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase

Gene

MGAT2

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes an essential step in the conversion of oligo-mannose to complex N-glycans.By similarity

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 6-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Pathway: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiREACT_337870. Reactions specific to the complex N-glycan synthesis pathway.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT16. Glycosyltransferase Family 16.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (EC:2.4.1.143)
Alternative name(s):
Beta-1,2-N-acetylglucosaminyltransferase II
GlcNAc-T II
Short name:
GNT-II
Mannoside acetylglucosaminyltransferase 2
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II
Gene namesi
Name:MGAT2
Synonyms:GNT2
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227 Componenti: Chromosome 1

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 99CytoplasmicSequence Analysis
Transmembranei10 – 2920Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini30 – 446417LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 446446Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferasePRO_0000080519Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi69 – 691N-linked (GlcNAc...)Sequence Analysis
Glycosylationi86 – 861N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Expressioni

Gene expression databases

GenevisibleiO19071. SS.

Interactioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000005385.

Family & Domainsi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG239633.
GeneTreeiENSGT00390000007341.
HOGENOMiHOG000261661.
HOVERGENiHBG052467.
InParanoidiO19071.
KOiK00736.
OMAiASVDFCQ.
OrthoDBiEOG7TF79W.
TreeFamiTF314772.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
InterProiIPR007754. GlcNAc_II.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR12871. PTHR12871. 1 hit.
PfamiPF05060. MGAT2. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.

Sequencei

Sequence statusi: Complete.

O19071-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRFRIYKRKV LILTFVVAAC GFVLWSSNGR QRKNEALAPP LLDAEPVRGA
60 70 80 90 100
GARAGDHPAI SVGIRRGSND SAAPLVAAAP QPEVDNLTLR YRSLVYQLNF
110 120 130 140 150
DQTLRNVDKV SSWVPRELVL VVQVHNRAEY LKLLLDSLRK AQGIDNVLVI
160 170 180 190 200
FSHDFWSTEI NQLIAGVDFC PVLQVFFPFS IQLYPNEFPG TDPRDCPRDL
210 220 230 240 250
EKNAALKMGC INAEYPDSFG HYREAKFSQT KHHWWWKLHF VWERVKVLRD
260 270 280 290 300
YAGLILFLEE DHYVAPDFYH VFKKMWNLKQ QECPECDVLS LGTYTTVRSF
310 320 330 340 350
RDVADKVDVK TWKSTEHNMG LALTRDAYQK LIECTDTFCT YDDYNWDWTL
360 370 380 390 400
QYLTVSCLPK FWKVLVPQVP RIFHAGDCGM HHKKTCRPST QSAQIESLLN
410 420 430 440
SNKQYMFPET LTISEKLTAA LSPPRKNGGW GDIRDHELCK SYRRLQ
Length:446
Mass (Da):51,527
Last modified:January 1, 1998 - v1
Checksum:i9B03F1C20778D897
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09537 Genomic DNA. Translation: CAA70732.1.
RefSeqiNP_001121954.1. NM_001128482.1.
UniGeneiSsc.43963.
Ssc.94564.

Genome annotation databases

EnsembliENSSSCT00000005522; ENSSSCP00000005385; ENSSSCG00000005006.
GeneIDi100151745.
KEGGissc:100151745.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09537 Genomic DNA. Translation: CAA70732.1.
RefSeqiNP_001121954.1. NM_001128482.1.
UniGeneiSsc.43963.
Ssc.94564.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000005385.

Protein family/group databases

CAZyiGT16. Glycosyltransferase Family 16.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000005522; ENSSSCP00000005385; ENSSSCG00000005006.
GeneIDi100151745.
KEGGissc:100151745.

Organism-specific databases

CTDi4247.

Phylogenomic databases

eggNOGiNOG239633.
GeneTreeiENSGT00390000007341.
HOGENOMiHOG000261661.
HOVERGENiHBG052467.
InParanoidiO19071.
KOiK00736.
OMAiASVDFCQ.
OrthoDBiEOG7TF79W.
TreeFamiTF314772.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiREACT_337870. Reactions specific to the complex N-glycan synthesis pathway.

Gene expression databases

GenevisibleiO19071. SS.

Family and domain databases

Gene3Di3.90.550.10. 2 hits.
InterProiIPR007754. GlcNAc_II.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR12871. PTHR12871. 1 hit.
PfamiPF05060. MGAT2. 1 hit.
[Graphical view]
SUPFAMiSSF53448. SSF53448. 2 hits.
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning of the porcine beta-1,2-N-acetylglucosaminyltransferase II gene and assignment to chromosome 1q23-q27."
    Leeb T., Kriegesmann B., Baumgartner B., Klett C., Yerle M., Hameister H., Brenig B.
    Biochim. Biophys. Acta 1336:361-366(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Brain and Liver.

Entry informationi

Entry nameiMGAT2_PIG
AccessioniPrimary (citable) accession number: O19071
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: January 1, 1998
Last modified: June 24, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.