O19071 (MGAT2_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
EC=2.4.1.143

Alternative name(s):
Beta-1,2-N-acetylglucosaminyltransferase II
GlcNAc-T II
Short name=GNT-II
Mannoside acetylglucosaminyltransferase 2
N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase II

Gene names

Name:	MGAT2
Synonyms:	GNT2

Organism

Sus scrofa (Pig) [Complete proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	446 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes an essential step in the conversion of oligo-mannose to complex N-glycans By similarity.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 6-(alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oligosaccharide biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell Golgi stack Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,6-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 446	446	Alpha-1,6-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase		PRO_0000080519
Regions
	Topological domain	1 – 9	9	Cytoplasmic Potential
	Transmembrane	10 – 29	20	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	30 – 446	417	Lumenal Potential
Amino acid modifications
	Glycosylation	69	1	N-linked (GlcNAc...) Potential
	Glycosylation	86	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O19071 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 9B03F1C20778D897	FASTA	446	51,527
	10 20 30 40 50 60 MRFRIYKRKV LILTFVVAAC GFVLWSSNGR QRKNEALAPP LLDAEPVRGA GARAGDHPAI 70 80 90 100 110 120 SVGIRRGSND SAAPLVAAAP QPEVDNLTLR YRSLVYQLNF DQTLRNVDKV SSWVPRELVL 130 140 150 160 170 180 VVQVHNRAEY LKLLLDSLRK AQGIDNVLVI FSHDFWSTEI NQLIAGVDFC PVLQVFFPFS 190 200 210 220 230 240 IQLYPNEFPG TDPRDCPRDL EKNAALKMGC INAEYPDSFG HYREAKFSQT KHHWWWKLHF 250 260 270 280 290 300 VWERVKVLRD YAGLILFLEE DHYVAPDFYH VFKKMWNLKQ QECPECDVLS LGTYTTVRSF 310 320 330 340 350 360 RDVADKVDVK TWKSTEHNMG LALTRDAYQK LIECTDTFCT YDDYNWDWTL QYLTVSCLPK 370 380 390 400 410 420 FWKVLVPQVP RIFHAGDCGM HHKKTCRPST QSAQIESLLN SNKQYMFPET LTISEKLTAA 430 440 LSPPRKNGGW GDIRDHELCK SYRRLQ

References

[1]

"Molecular cloning of the porcine beta-1,2-N-acetylglucosaminyltransferase II gene and assignment to chromosome 1q23-q27." Leeb T., Kriegesmann B., Baumgartner B., Klett C., Yerle M., Hameister H., Brenig B. Biochim. Biophys. Acta 1336:361-366(1997) [PubMed: 9367162] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Brain and Liver.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y09537 Genomic DNA. Translation: CAA70732.1.
RefSeq	NP_001121954.1. NM_001128482.1.
UniGene	Ssc.43963. Ssc.94564.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O19071.
Protein family/group databases
CAZy	GT16. Glycosyltransferase Family 16.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSSSCT00000005522; ENSSSCP00000005385; ENSSSCG00000005006.
GeneID	100151745.
KEGG	ssc:100151745.
Organism-specific databases
CTD	4247.
Phylogenomic databases
GeneTree	ENSGT00390000007341.
HOVERGEN	HBG052467.
OMA	GDCGMHH.
OrthoDB	EOG4VX255.
Family and domain databases
InterPro	IPR007754. GlcNAc_II. [Graphical view]
KO	K00736.
PANTHER	PTHR12871. MGAT2. 1 hit.
Pfam	PF05060. MGAT2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MGAT2_PIG

Accession

Primary (citable) accession number: O19071

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 10, 2004
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program