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O19069 (SUCA_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial
EC=6.2.1.4
EC=6.2.1.5
Alternative name(s):
Succinyl-CoA synthetase subunit alpha
Short name=SCS-alpha
Gene names
Name:SUCLG1
OrganismSus scrofa (Pig) [Complete proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the ATP- or GTP-dependent ligation of succinate and CoA to form succinyl-CoA. The nature of the beta subunit determines the nucleotide specificity By similarity.

Catalytic activity

GTP + succinate + CoA = GDP + phosphate + succinyl-CoA.

ATP + succinate + CoA = ADP + phosphate + succinyl-CoA.

Pathway

Carbohydrate metabolism; tricarboxylic acid cycle.

Subunit structure

Heterodimer of an alpha and a beta subunit.

Subcellular location

Mitochondrion By similarity.

Tissue specificity

Isoform I is expressed in all tissues examined: liver, brain, heart, and skeletal muscle. Isoform II is expressed only in heart and skeletal muscle.

Sequence similarities

Belongs to the succinate/malate CoA ligase alpha subunit family.

Sequence caution

The sequence AAB94003.1 differs from that shown. Reason: Erroneous initiation.

The sequence AAB94004.1 differs from that shown. Reason: Erroneous initiation.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform I (identifier: O19069-2)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform II (identifier: O19069-1)

The sequence of this isoform differs from the canonical sequence as follows:
     178-196: PGECKIGIMPGHIHKKGRI → RFLRFAVYITSRVFTCTQQEEYRKIPLLFGTRSIHM

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4040Mitochondrion By similarity
Chain41 – 346306Succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial
PRO_0000033342

Sites

Active site2991Tele-phosphohistidine intermediate By similarity

Amino acid modifications

Modified residue541N6-acetyllysine By similarity
Cross-link280Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity

Natural variations

Alternative sequence178 – 19619PGECK…KKGRI → RFLRFAVYITSRVFTCTQQE EYRKIPLLFGTRSIHM in isoform II.
VSP_036394

Experimental info

Sequence conflict2 – 43TAA → PAG in AAB94003. Ref.2
Sequence conflict2 – 43TAA → PAG in AAB94004. Ref.2

Secondary structure

1 ............................... 346
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform I [UniParc].

Last modified February 10, 2009. Version 2.
Checksum: C5ACA670C214C2D4

FASTA34636,117
        10         20         30         40         50         60 
MTAALVAAPA AATMASGSSG LAAARLLSRS FLLQQNGIRH CSYTASRKHL YVDKNTKVIC 

        70         80         90        100        110        120 
QGFTGKQGTF HSQQALEYGT NLVGGTTPGK GGKTHLGLPV FNTVKEAKEQ TGATASVIYV 

       130        140        150        160        170        180 
PPPFAAAAIN EAIDAEVPLV VCITEGIPQQ DMVRVKHRLL RQGKTRLIGP NCPGVINPGE 

       190        200        210        220        230        240 
CKIGIMPGHI HKKGRIGIVS RSGTLTYEAV HQTTQVGLGQ SLCVGIGGDP FNGTDFTDCL 

       250        260        270        280        290        300 
EIFLNDPATE GIILIGEIGG NAEENAAEFL KQHNSGPKSK PVVSFIAGLT APPGRRMGHA 

       310        320        330        340 
GAIIAGGKGG AKEKITALQS AGVVVSMSPA QLGTTIYKEF EKRKML

« Hide

Isoform II [UniParc].

Checksum: 537EAA9D870E9C01
Show »

FASTA36338,454

References

« Hide 'large scale' references
[1]"PEDE (Pig EST Data Explorer): construction of a database for ESTs derived from porcine full-length cDNA libraries."
Uenishi H., Eguchi T., Suzuki K., Sawazaki T., Toki D., Shinkai H., Okumura N., Hamasima N., Awata T.
Nucleic Acids Res. 32:D484-D488(2004) [PubMed: 14681463] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-291 (ISOFORM I).
[2]"Mutually exclusive splicing generates two distinct isoforms of pig heart succinyl-CoA synthetase."
Ryan D.G., Lin T., Brownie E., Bridger W.A., Wolodko W.T.
J. Biol. Chem. 272:21151-21159(1997) [PubMed: 9261120] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2-346 (ISOFORMS I AND II), NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 133-234, ALTERNATIVE SPLICING.
Tissue: Heart.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BP167826 mRNA. No translation available.
AF008432, AF008430 Genomic DNA. Translation: AAB94001.1.
AF008433, AF008430, AF008431 Genomic DNA. Translation: AAB94002.2.
AF008588 mRNA. Translation: AAB94003.1. Different initiation.
AF008589 mRNA. Translation: AAB94004.1. Different initiation.
RefSeqNP_999574.1. NM_214409.1.
UniGeneSsc.14502.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1EUCX-ray2.10A38-333[»]
1EUDX-ray2.10A38-346[»]
2FP4X-ray2.08A42-346[»]
2FPGX-ray2.96A42-346[»]
2FPIX-ray2.70A42-346[»]
2FPPX-ray2.35A42-346[»]
ProteinModelPortalO19069.
ModBaseSearch...

Protein-protein interaction databases

STRINGO19069.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID399539.
KEGGssc:399539.

Organism-specific databases

CTD8802.

Phylogenomic databases

GeneTreeENSGT00530000063275.
HOVERGENHBG000957.
OrthoDBEOG4WQ136.

Family and domain databases

InterProIPR017440. Cit_synth/succinyl-CoA_lig_AS.
IPR003781. CoA-bd.
IPR005810. CoA_lig_alpha.
IPR005811. CoA_ligase.
IPR016040. NAD(P)-bd_dom.
IPR016102. Succinyl-CoA_synth-like.
[Graphical view]
Gene3DG3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
G3DSA:3.40.50.261. Succinyl-CoA_synth-like. 1 hit.
KOK01899.
PfamPF02629. CoA_binding. 1 hit.
PF00549. Ligase_CoA. 1 hit.
[Graphical view]
PIRSFPIRSF001553. SucCS_alpha. 1 hit.
PRINTSPR01798. SCOASYNTHASE.
SMARTSM00881. CoA_binding. 1 hit.
[Graphical view]
SUPFAMSSF52210. CoA_ligase. 1 hit.
TIGRFAMsTIGR01019. SucCoAalpha. 1 hit.
PROSITEPS01216. SUCCINYL_COA_LIG_1. 1 hit.
PS00399. SUCCINYL_COA_LIG_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSUCA_PIG
AccessionPrimary (citable) accession number: O19069
Secondary accession number(s): O19068
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 10, 2009
Last modified: November 16, 2011
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents