Reviewed, UniProtKB/Swiss-Prot O19064 (JAK2_PIG)

Names and origin

Protein names

Recommended name:
    Tyrosine-protein kinase JAK2
    EC=2.7.10.2
Alternative name(s):
    Janus kinase 2
      Short name=JAK-2

Gene names

Name:

JAK2

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	1131 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Tyrosine kinase of the non-receptor type, involved in interleukin-3 and probably interleukin-23 signal transduction. Plays a role in leptin signaling and control of body weight By similarity.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure	Interacts with IL23R, SIRPA, SH2B1, SKB1 and STAM2 By similarity.
Subcellular location	Endomembrane system; Peripheral membrane protein By similarity. Note: Wholly intracellular, possibly membrane associated By similarity.
Domain	Possesses two phosphotransferase domains. The second one probably contains the catalytic domain By similarity, while the presence of slight differences suggest a different role for domain 1.
Post-translational modification	Leptin promotes phosphorylation on tyrosine residues, including phosphorylation on Tyr-813 By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily. Contains 1 FERM domain. Contains 1 protein kinase domain. Contains 1 SH2 domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat SH2 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoskeleton Inferred from electronic annotation. Source: InterPro extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW Janus kinase activity Inferred from electronic annotation. Source: InterPro non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1131	1131	Tyrosine-protein kinase JAK2		PRO_0000324093
Regions
	Domain	37 – 380	344	FERM
	Domain	401 – 482	82	SH2; atypical
	Domain	545 – 809	265	Protein kinase 1
	Domain	849 – 1126	278	Protein kinase 2
	Nucleotide binding	855 – 863	9	ATP By similarity
Sites
	Active site	976	1	Proton acceptor By similarity
	Binding site	882	1	ATP By similarity
Amino acid modifications
	Modified residue	523	1	Phosphoserine By similarity
	Modified residue	570	1	Phosphotyrosine By similarity
	Modified residue	813	1	Phosphotyrosine By similarity
	Modified residue	1007	1	Phosphotyrosine; by autocatalysis By similarity
	Modified residue	1008	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O19064-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 6339C7417087EB2C	FASTA	1,131	130,759
	10 20 30 40 50 60 MGMACLTMTE MEGTSTSPVH QNGDIPGNAN SVKQIDPVLQ VYLYHSLGKA EGDYLKFPAG 70 80 90 100 110 120 EYVAEEICVA ASKACGITPV YHSMFALMNE TERIWYPPNH VFHVDESTRH NVLYRIRFYF 130 140 150 160 170 180 PYWYCNGSNR TYRHGISRGA EAPLLDDFVM SYLFAQWRHD FLYGWVKIPV THETQEECLG 190 200 210 220 230 240 MAVLDMMRIA KEKDQTPLDI YSSVSYKTFL PKCVRAKIQD YHILTRKRIR YRFRRFIEQF 250 260 270 280 290 300 SHCKATARNL KLKYLINLET LQSAFYTEQF EVKEPGRGPS GEEIFATIII TGNGGIQWSR 310 320 330 340 350 360 GKHKESETLT EQDLQLYCDF PDIIDVSIKQ ANQEGSNESR IVTIHKQDGK SLEIELSSLR 370 380 390 400 410 420 EALSFVSLID GYYRLTADAH HYLCKEVAPP MVLENIQSNC HGPISMDFAI SKLKKAGNQT 430 440 450 460 470 480 GLFVLRCSPK DFNKYFLTFA VERENVTEYK HCLITKNENG EYNLSGTRKN FSNLKDLLNC 490 500 510 520 530 540 YQMETVRSDS IIFQFTKCCP PKPKDKSNLL VFRTNGISDV PTSPTLQRHN NVNQMVFHKI 550 560 570 580 590 600 RNEDLIFNES LGQGTFTKIF KGVRREVGDY GQLHETEVLL KVLDKAHRNY SESFFEAASM 610 620 630 640 650 660 MSQLSHKHLV LNYGVCVCGE ENILVQEFVK FGSLDTYLKK NKNSINILWK LEVAKQLAWA 670 680 690 700 710 720 MHFLEEKTLI HGNVCAKNIL LIREEDRKTG NPPFIKLSDP GISITVLPKD ILQERIPWVP 730 740 750 760 770 780 PECIENPKNL NLATDKWSFG TTLWEICSGG DKPLNALDSQ RKLQFYEDRH QLPAPKWTEL 790 800 810 820 830 840 ANLINNCMDY EPDFRPSFRA IIRDLNSLFT PDYELLTEND MLPNMRIGAL GFSGAFEDRD 850 860 870 880 890 900 PTQFEERHLK FLQQLGKGNF GSVEMCRYDP LQDNTGEVVA VKKLQHSTEE HLRDFEREIE 910 920 930 940 950 960 ILKSLQHDNI VKYKGVCYSA GRRNLRLIME YLPYGSLRDY LQKHKERIDH KKLLQYTSQI 970 980 990 1000 1010 1020 CKGMEYLGTK RYIHRDLATR NILVENENRV KIGDFGLTKV LPQDKEYYKV KEPGESPIFW 1030 1040 1050 1060 1070 1080 YAPESLTESK FSVASDVWSF GVVLYELFTY IEKSKSPPAE FMRMIGNDKQ GQMIVFHLIE 1090 1100 1110 1120 1130 LLKNNGRLPR PDGCPDEIYI IMTECWNNNV NQRPSFRDLA LRVDQIRDSM A

References

[1]	"Domestic pig mRNA for JAK2, complete cds." Ito Y., Mikawa S., Kobayashi E., Wada Y., Minezawa M. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Muscle.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB006011 mRNA. Translation: BAA21662.1.
RefSeq	NP_999278.1.
UniGene	Ssc.324
3D structure databases
HSSP	HSSP built from PDB template 1R1W based on UniProtKB P08581.
SMR	O19064. Positions 840-1131.
ModBase	Search...
Genome annotation databases
GeneID	397201.
KEGG	ssc:397201.
Organism-specific databases
CTD	397201.
Phylogenomic databases
HOVERGEN	O19064.
Enzyme and pathway databases
BRENDA	2.7.10.2. 249.
Family and domain databases
InterPro	IPR019749. Band_41_domain. IPR019747. FERM_CS. IPR000299. FERM_domain. IPR009127. JAK. IPR009129. JAK2. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR000980. SH2. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. IPR016251. TyrPK_Jak. [Graphical view]
Gene3D	G3DSA:3.30.505.10. SH2. 1 hit.
Pfam	PF07714. Pkinase_Tyr. 2 hits. PF00017. SH2. 1 hit. [Graphical view]
PIRSF	PIRSF000636. TyrPK_Jak. 1 hit.
PRINTS	PR01823. JANUSKINASE. PR01825. JANUSKINASE2. PR00109. TYRKINASE.
ProDom	PD000001. Prot_kinase. 2 hits. PD000093. SH2. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00295. B41. 1 hit. SM00252. SH2. 1 hit. SM00219. TyrKc. 2 hits. [Graphical view]
PROSITE	PS00660. FERM_1. False negative. PS00661. FERM_2. False negative. PS50057. FERM_3. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 2 hits. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

JAK2_PIG

Accession

Primary (citable) accession number: O19064

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	January 1, 1998
Last modified:	October 13, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)