ID   LOX12_RABIT             Reviewed;         663 AA.
AC   O19043;
DT   11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   16-JUN-2009, entry version 61.
DE   RecName: Full=Arachidonate 12-lipoxygenase, 12S-type;
DE            Short=12-LOX;
DE            EC=1.13.11.31;
GN   Name=ALOX12;
OS   Oryctolagus cuniculus (Rabbit).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Lagomorpha; Leporidae;
OC   Oryctolagus.
OX   NCBI_TaxID=9986;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RX   MEDLINE=98263260; PubMed=9600854; DOI=10.1006/jmbi.1998.1737;
RA   Berger M., Schwarz K., Thiele H., Reimann I., Huth A., Borngraeber S.,
RA   Kuehn H., Thiele B.-J.;
RT   "Simultaneous expression of leukocyte-type 12-lipoxygenase and
RT   reticulocyte-type 15-lipoxygenase in rabbits.";
RL   J. Mol. Biol. 278:935-948(1998).
CC   -!- FUNCTION: Oxygenase and 14,15-leukotriene A4 synthase activity.
CC   -!- CATALYTIC ACTIVITY: Arachidonate + O(2) = (5Z,8Z,10E,14Z)-(12S)-
CC       12-hydroperoxyicosa-5,8,10,14-tetraenoate.
CC   -!- COFACTOR: Binds 1 iron ion per subunit.
CC   -!- PATHWAY: Lipid metabolism; leukotriene D4 biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the lipoxygenase family.
CC   -!- SIMILARITY: Contains 1 lipoxygenase domain.
CC   -!- SIMILARITY: Contains 1 PLAT domain.
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DR   EMBL; Z97654; CAB10746.1; -; mRNA.
DR   RefSeq; NP_001139620.1; -.
DR   HSSP; P12530; 1LOX.
DR   SMR; O19043; 2-663.
DR   GeneID; 100271999; -.
DR   HOVERGEN; O19043; -.
DR   BRENDA; 1.13.11.31; 255.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004052; F:arachidonate 12-lipoxygenase activity; IEA:EC.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0016165; F:lipoxygenase activity; IEA:InterPro.
DR   GO; GO:0019370; P:leukotriene biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR000907; LipOase.
DR   InterPro; IPR013819; LipOase_C.
DR   InterPro; IPR001024; LipOase_LH2.
DR   InterPro; IPR001885; LipOase_mml.
DR   Gene3D; G3DSA:2.60.60.20; Lipase_LipOase; 1.
DR   PANTHER; PTHR11771; LipOase; 1.
DR   Pfam; PF00305; Lipoxygenase; 1.
DR   Pfam; PF01477; PLAT; 1.
DR   PRINTS; PR00087; LIPOXYGENASE.
DR   PRINTS; PR00467; MAMLPOXGNASE.
DR   SMART; SM00308; LH2; 1.
DR   PROSITE; PS00711; LIPOXYGENASE_1; 1.
DR   PROSITE; PS00081; LIPOXYGENASE_2; 1.
DR   PROSITE; PS51393; LIPOXYGENASE_3; 1.
DR   PROSITE; PS50095; PLAT; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Dioxygenase; Iron; Leukotriene biosynthesis; Metal-binding;
KW   Oxidoreductase.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    663       Arachidonate 12-lipoxygenase, 12S-type.
FT                                /FTId=PRO_0000220685.
FT   DOMAIN        2    115       PLAT.
FT   DOMAIN      116    663       Lipoxygenase.
FT   METAL       361    361       Iron; catalytic (By similarity).
FT   METAL       366    366       Iron; catalytic (By similarity).
FT   METAL       541    541       Iron; catalytic (By similarity).
FT   METAL       663    663       Iron; via carboxylate; catalytic (By
FT                                similarity).
SQ   SEQUENCE   663 AA;  75234 MW;  19670402E5C22A8B CRC64;
     MGVYRVCVST GASIYAGSKN KVELWLVGQH GEVELGSCLR PSRNKEEEFK VNVSKYLGSL
     LFVKLRKKHF LKEDAWFCNW ISVQALGAAE DKYWFPCYRW VVGDGVQSLP VGTGCTTVGD
     PQGLFQKHRE QELEERRKLY QWGSWKEGLI LNVAGSKLTD LPVDERFLED KKIDFEASLA
     WGLAELALKN SLNILAPWKT LDDFNRIFWC GRSKLARRVR DSWQEDSLFG YQFLNGANPM
     LLRRSVQLPA RLVFPPGMEE LQAQLEKELK AGTLFEADFA LLDNIKANVI LYCQQYLAAP
     LVMLKLQPDG KLMPMVIQLH LPKIGSSPPP LFLPTDPPMV WLLAKCWVRS SDLQVHELNS
     HLLRGHLMAE VFTVATMRCL PSIHPVFKLI VPHLRYTLEI NVRARNGLVS DFGIFDQIMS
     TGGGGHVQLL QQAGAFLTYR SFCPPDDLAD RGLLGVESSF YAQDALRLWE IISRYVQGIM
     GLYYKTDEAV RDDLELQSWC REITEIGLQG AQKQGFPTSL QSVAQACHFV TMCIFTCTGQ
     HSSIHLGQLD WFTWVPNAPC TMRLPPPTTK DATLETVMAT LPNLHQSSLQ MSIVWQLGRD
     QPIMVPLGQH QEEYFSGPEP RAVLEKFREE LAIMDKEIEV RNEKLDIPYE YLRPSIVENS
     VAI
//