Reviewed, UniProtKB/Swiss-Prot O19023 (ELA3B_MACMU)

Names and origin

Protein names

Recommended name:
    Elastase-3B
    EC=3.4.21.70
Alternative name(s):
    Elastase IIIB
    Protease E

Gene names

Name:

ELA3B

Organism

Macaca mulatta (Rhesus macaque)

Taxonomic identifier

9544 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Cercopithecidae › Cercopithecinae › Macaca

Protein attributes

Sequence length	257 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Efficient protease with alanine specificity but only little elastolytic activity By similarity.
Catalytic activity	Preferential cleavage: Ala-|-Xaa. Does not hydrolyze elastin.
Sequence similarities	Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	‹1 – 2	›2	Or 3 Potential
	Propeptide	3 – 15	13	Activation peptide Potential		PRO_0000027701
	Chain	16 – ›257	›242	Elastase-3B		PRO_0000027702
Regions
	Domain	16 – 255	240	Peptidase S1
Sites
	Active site	60	1	Charge relay system By similarity
	Active site	110	1	Charge relay system By similarity
	Active site	204	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	38	1	N-linked (GlcNAc...) Potential
	Disulfide bond	45 ↔ 61		By similarity
	Disulfide bond	104 ↔ 107		By similarity
	Disulfide bond	144 ↔ 210		By similarity
	Disulfide bond	175 ↔ 191		By similarity
	Disulfide bond	200 ↔ 231		By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	257	1

Sequences

	Sequence		Length	Mass (Da)
	O19023-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 4D443DB67233D8DC	FASTA	257	27,687
	10 20 30 40 50 60 VASGYGPPSS HPSSRVVNGE DAVPYSWPWQ VSLQYEKNGS FHHTCGGSLI APDWVVTAGH 70 80 90 100 110 120 CISSSLTYQV ALGDYNLAVK EGPEQVIPIN SGDLFVHPLW NRLCVACGND IALIKLSRSA 130 140 150 160 170 180 QLGDAVQLAS LPPAGDILPN ETPCYITGWG RLYTNGPLPD KLQRALLPVV DYEHCSKWNW 190 200 210 220 230 240 WGSTVKKTMV CAGGDIRSGC NGDSGGPLNC PTDDGGWQVH GVTSFVSSFG CNTQRKPTVF 250 TRVSAFIDWI EETIASH

References

[1]	"Pancreatic elastase from rhesus monkey." Sziegoleit A. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreas.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ000067 mRNA. Translation: CAA03899.1.
UniGene	Mmu.3417
3D structure databases
HSSP	HSSP built from PDB template 1PYT based on UniProtKB P05805.
SMR	O19023. Positions 10-257.
ModBase	Search...
Protein family/group databases
MEROPS	S01.154.
Genome annotation databases
Ensembl	ENSMMUG00000003887. Macaca mulatta. [Contig view]
Phylogenomic databases
HOVERGEN	O19023.
Enzyme and pathway databases
BRENDA	3.4.21.70. 1774.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ELA3B_MACMU

Accession

Primary (citable) accession number: O19023

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	January 1, 1998
Last modified:	March 3, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)