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Protein

Beta-galactosidase

Gene

GLB1

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei189 – 1891Proton donorBy similarity
Active sitei269 – 2691NucleophileSequence Analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.23. 2235.

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23)
Alternative name(s):
Acid beta-galactosidase
Short name:
Lactase
Gene namesi
Name:GLB1
Synonyms:BGAL
OrganismiFelis catus (Cat) (Felis silvestris catus)
Taxonomic identifieri9685 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
ProteomesiUP000011712 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Propeptidei25 – 295By similarityPRO_0000012183
Chaini30 – 669640Beta-galactosidasePRO_0000012184Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi27 – 271N-linked (GlcNAc...)Sequence Analysis
Glycosylationi248 – 2481N-linked (GlcNAc...)Sequence Analysis
Glycosylationi465 – 4651N-linked (GlcNAc...)Sequence Analysis
Glycosylationi499 – 4991N-linked (GlcNAc...)Sequence Analysis
Glycosylationi547 – 5471N-linked (GlcNAc...)Sequence Analysis
Glycosylationi557 – 5571N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein, Zymogen

Proteomic databases

PRIDEiO19015.

Structurei

3D structure databases

ProteinModelPortaliO19015.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG004841.
InParanoidiO19015.
KOiK12309.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR026283. B-gal_1-like.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF13364. BetaGal_dom4_5. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PIRSFiPIRSF006336. B-gal. 1 hit.
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O19015-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFPGAARLL SLLLVPLLLG PARGLRNASQ RTFKIDYGHN RFLKDGQPFR
60 70 80 90 100
YISGSIHYFR VPRFYWKDRL LKMKMAGLNA IQTYVPWNFH EPQPGQYQFS
110 120 130 140 150
GEHDVEYFLK LAHELGLLVI LRPGPYICAE WDMGGLPAWL LLKESIILRS
160 170 180 190 200
SDPDYLAAVD KWLGVLLPKM KPLLYQNGGP IITVQVENEY GSYFTCDYDY
210 220 230 240 250
LRFLQRRFRD HLGGDVLLFT TDGAHEKFLQ CGALQGIYAT VDFGPDANIT
260 270 280 290 300
AAFQIQRKSE PRGPLVNSEF YTGWLDHWGQ PHSRVRTEVV ASSLHDVLAH
310 320 330 340 350
GANVNLYMFI GGTNFAYWNG ANIPYQPQPT SYDYDAPLSE AGDLTDKYFA
360 370 380 390 400
LRDVIRKFEK VPEGFIPPST PKFAYGKVAL QKLKTVEDAL NVLCPAGPIK
410 420 430 440 450
SLYPLTFIQV KQYFGFVLYR TTLPQDCSNP TPLSSPLNGV RDRAYVAVDG
460 470 480 490 500
VPQGVLERSY VITLNITGQA GATLDLLVEN MGRVNYGRYI NDFKGLISNL
510 520 530 540 550
TLGSSVLTDW MIFPLDTEDA VRSHLGGWHG RNHGRQDNKA FAHHSSNYTL
560 570 580 590 600
PAFYAGNFSI PSGIPDLPQD TFIQFSGWTK GQVWINGFNL GRYWPGRGPQ
610 620 630 640 650
VTLFVPRHIL VTSAPNTIMV LELERAPCDD NGPELCTVEF VDRPLISATP
660
TSSHPLPDLS DRDSGWDRV
Length:669
Mass (Da):75,230
Last modified:January 1, 1998 - v1
Checksum:i35B84933BB5E2F76
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti483 – 4831R → P in AAB86405 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006749 mRNA. Translation: AAB81350.1.
AF029974 mRNA. Translation: AAB86405.1.
RefSeqiNP_001009860.1. NM_001009860.1.

Genome annotation databases

GeneIDi493927.
KEGGifca:493927.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006749 mRNA. Translation: AAB81350.1.
AF029974 mRNA. Translation: AAB86405.1.
RefSeqiNP_001009860.1. NM_001009860.1.

3D structure databases

ProteinModelPortaliO19015.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

ChEMBLiCHEMBL2331052.

Protein family/group databases

CAZyiGH35. Glycoside Hydrolase Family 35.

Proteomic databases

PRIDEiO19015.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi493927.
KEGGifca:493927.

Organism-specific databases

CTDi2720.

Phylogenomic databases

HOVERGENiHBG004841.
InParanoidiO19015.
KOiK12309.

Enzyme and pathway databases

BRENDAi3.2.1.23. 2235.

Miscellaneous databases

PROiO19015.

Family and domain databases

Gene3Di2.60.120.260. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR026283. B-gal_1-like.
IPR025300. BetaGal_jelly_roll_dom.
IPR008979. Galactose-bd-like.
IPR019801. Glyco_hydro_35_CS.
IPR013781. Glyco_hydro_catalytic_dom.
IPR001944. Glycoside_Hdrlase_35.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR23421. PTHR23421. 1 hit.
PfamiPF13364. BetaGal_dom4_5. 1 hit.
PF01301. Glyco_hydro_35. 1 hit.
[Graphical view]
PIRSFiPIRSF006336. B-gal. 1 hit.
PRINTSiPR00742. GLHYDRLASE35.
SUPFAMiSSF49785. SSF49785. 2 hits.
SSF51445. SSF51445. 1 hit.
PROSITEiPS01182. GLYCOSYL_HYDROL_F35. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The sequence of feline lysosomal beta-galactosidase."
    Varadarajan G.S., Smith B.F., Foureman P., Martin D.R., Varadarajan U., Georgeson M., Baker H.J.
    Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The mutation in feline beta-galactosidase deficiency (GM1 gangliosidosis)."
    Smith B.F., Foureman P., Georgeson M., Martin D.R., Baker H.J.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiBGAL_FELCA
AccessioniPrimary (citable) accession number: O19015
Secondary accession number(s): O18898
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: May 27, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.