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Protein

Beta-galactosidase

Gene

GLB1

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.By similarity

Catalytic activityi

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei84SubstrateBy similarity1
Binding sitei130SubstrateBy similarity1
Binding sitei188SubstrateBy similarity1
Active sitei189Proton donorBy similarity1
Active sitei269NucleophileBy similarity1
Binding sitei334SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidase, Hydrolase

Enzyme and pathway databases

BRENDAi3.2.1.23 2235

Protein family/group databases

CAZyiGH35 Glycoside Hydrolase Family 35

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-galactosidase (EC:3.2.1.23By similarity)
Alternative name(s):
Acid beta-galactosidase
Short name:
Lactase
Gene namesi
Name:GLB1
Synonyms:BGAL
OrganismiFelis catus (Cat) (Felis silvestris catus)
Taxonomic identifieri9685 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
Proteomesi
  • UP000011712 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2331052

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000001218325 – 29By similarity5
ChainiPRO_000001218430 – 669Beta-galactosidaseAdd BLAST640

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi27N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi196 ↔ 231By similarity
Glycosylationi248N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi465N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi499N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi547N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi557N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi628 ↔ 636By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PRIDEiO19015

Interactioni

Subunit structurei

Homodimer. May form higher multimers.By similarity

Protein-protein interaction databases

STRINGi9685.ENSFCAP00000019661

Structurei

3D structure databases

ProteinModelPortaliO19015
SMRiO19015
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 35 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG0496 Eukaryota
COG1874 LUCA
HOVERGENiHBG004841
InParanoidiO19015
KOiK12309

Family and domain databases

Gene3Di2.60.120.260, 3 hits
InterProiView protein in InterPro
IPR026283 B-gal_1-like
IPR025300 BetaGal_jelly_roll_dom
IPR008979 Galactose-bd-like_sf
IPR031330 Gly_Hdrlase_35_cat
IPR019801 Glyco_hydro_35_CS
IPR001944 Glycoside_Hdrlase_35
IPR017853 Glycoside_hydrolase_SF
PANTHERiPTHR23421 PTHR23421, 1 hit
PfamiView protein in Pfam
PF13364 BetaGal_dom4_5, 1 hit
PF01301 Glyco_hydro_35, 1 hit
PIRSFiPIRSF006336 B-gal, 1 hit
PRINTSiPR00742 GLHYDRLASE35
SUPFAMiSSF49785 SSF49785, 2 hits
SSF51445 SSF51445, 1 hit
PROSITEiView protein in PROSITE
PS01182 GLYCOSYL_HYDROL_F35, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O19015-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDFPGAARLL SLLLVPLLLG PARGLRNASQ RTFKIDYGHN RFLKDGQPFR
60 70 80 90 100
YISGSIHYFR VPRFYWKDRL LKMKMAGLNA IQTYVPWNFH EPQPGQYQFS
110 120 130 140 150
GEHDVEYFLK LAHELGLLVI LRPGPYICAE WDMGGLPAWL LLKESIILRS
160 170 180 190 200
SDPDYLAAVD KWLGVLLPKM KPLLYQNGGP IITVQVENEY GSYFTCDYDY
210 220 230 240 250
LRFLQRRFRD HLGGDVLLFT TDGAHEKFLQ CGALQGIYAT VDFGPDANIT
260 270 280 290 300
AAFQIQRKSE PRGPLVNSEF YTGWLDHWGQ PHSRVRTEVV ASSLHDVLAH
310 320 330 340 350
GANVNLYMFI GGTNFAYWNG ANIPYQPQPT SYDYDAPLSE AGDLTDKYFA
360 370 380 390 400
LRDVIRKFEK VPEGFIPPST PKFAYGKVAL QKLKTVEDAL NVLCPAGPIK
410 420 430 440 450
SLYPLTFIQV KQYFGFVLYR TTLPQDCSNP TPLSSPLNGV RDRAYVAVDG
460 470 480 490 500
VPQGVLERSY VITLNITGQA GATLDLLVEN MGRVNYGRYI NDFKGLISNL
510 520 530 540 550
TLGSSVLTDW MIFPLDTEDA VRSHLGGWHG RNHGRQDNKA FAHHSSNYTL
560 570 580 590 600
PAFYAGNFSI PSGIPDLPQD TFIQFSGWTK GQVWINGFNL GRYWPGRGPQ
610 620 630 640 650
VTLFVPRHIL VTSAPNTIMV LELERAPCDD NGPELCTVEF VDRPLISATP
660
TSSHPLPDLS DRDSGWDRV
Length:669
Mass (Da):75,230
Last modified:January 1, 1998 - v1
Checksum:i35B84933BB5E2F76
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti483R → P in AAB86405 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF006749 mRNA Translation: AAB81350.1
AF029974 mRNA Translation: AAB86405.1
RefSeqiNP_001009860.1, NM_001009860.1

Genome annotation databases

GeneIDi493927
KEGGifca:493927

Similar proteinsi

Entry informationi

Entry nameiBGAL_FELCA
AccessioniPrimary (citable) accession number: O19015
Secondary accession number(s): O18898
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
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