O19015 (BGAL_FELCA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Beta-galactosidase
EC=3.2.1.23

Alternative name(s):
Acid beta-galactosidase
Short name=Lactase

Gene names

Name:	GLB1
Synonyms:	BGAL

Organism

Felis catus (Cat) (Felis silvestris catus)

Taxonomic identifier

9685 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Feliformia › Felidae › Felinae › Felis

Protein attributes

Sequence length	669 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cleaves beta-linked terminal galactosyl residues from gangliosides, glycoproteins, and glycosaminoglycans.
Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides.
Subcellular location	Lysosome.
Sequence similarities	Belongs to the glycosyl hydrolase 35 family.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Propeptide	25 – 29	5	By similarity		PRO_0000012183
	Chain	30 – 669	640	Beta-galactosidase		PRO_0000012184
Sites
	Active site	189	1	Proton donor By similarity
	Active site	269	1	Nucleophile Potential
Amino acid modifications
	Glycosylation	27	1	N-linked (GlcNAc...) Potential
	Glycosylation	248	1	N-linked (GlcNAc...) Potential
	Glycosylation	465	1	N-linked (GlcNAc...) Potential
	Glycosylation	499	1	N-linked (GlcNAc...) Potential
	Glycosylation	547	1	N-linked (GlcNAc...) Potential
	Glycosylation	557	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	483	1	R → P in AAB86405. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O19015 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 35B84933BB5E2F76	FASTA	669	75,230
	10 20 30 40 50 60 MDFPGAARLL SLLLVPLLLG PARGLRNASQ RTFKIDYGHN RFLKDGQPFR YISGSIHYFR 70 80 90 100 110 120 VPRFYWKDRL LKMKMAGLNA IQTYVPWNFH EPQPGQYQFS GEHDVEYFLK LAHELGLLVI 130 140 150 160 170 180 LRPGPYICAE WDMGGLPAWL LLKESIILRS SDPDYLAAVD KWLGVLLPKM KPLLYQNGGP 190 200 210 220 230 240 IITVQVENEY GSYFTCDYDY LRFLQRRFRD HLGGDVLLFT TDGAHEKFLQ CGALQGIYAT 250 260 270 280 290 300 VDFGPDANIT AAFQIQRKSE PRGPLVNSEF YTGWLDHWGQ PHSRVRTEVV ASSLHDVLAH 310 320 330 340 350 360 GANVNLYMFI GGTNFAYWNG ANIPYQPQPT SYDYDAPLSE AGDLTDKYFA LRDVIRKFEK 370 380 390 400 410 420 VPEGFIPPST PKFAYGKVAL QKLKTVEDAL NVLCPAGPIK SLYPLTFIQV KQYFGFVLYR 430 440 450 460 470 480 TTLPQDCSNP TPLSSPLNGV RDRAYVAVDG VPQGVLERSY VITLNITGQA GATLDLLVEN 490 500 510 520 530 540 MGRVNYGRYI NDFKGLISNL TLGSSVLTDW MIFPLDTEDA VRSHLGGWHG RNHGRQDNKA 550 560 570 580 590 600 FAHHSSNYTL PAFYAGNFSI PSGIPDLPQD TFIQFSGWTK GQVWINGFNL GRYWPGRGPQ 610 620 630 640 650 660 VTLFVPRHIL VTSAPNTIMV LELERAPCDD NGPELCTVEF VDRPLISATP TSSHPLPDLS DRDSGWDRV

References

[1]	"The sequence of feline lysosomal beta-galactosidase." Varadarajan G.S., Smith B.F., Foureman P., Martin D.R., Varadarajan U., Georgeson M., Baker H.J. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The mutation in feline beta-galactosidase deficiency (GM1 gangliosidosis)." Smith B.F., Foureman P., Georgeson M., Martin D.R., Baker H.J. Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF006749 mRNA. Translation: AAB81350.1. AF029974 mRNA. Translation: AAB86405.1.
RefSeq	NP_001009860.1. NM_001009860.1.
3D structure databases
ProteinModelPortal	O19015.
ModBase	Search...
Protein family/group databases
CAZy	GH35. Glycoside Hydrolase Family 35.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	493927.
Organism-specific databases
CTD	2720.
Phylogenomic databases
eggNOG	maNOG14643.
HOVERGEN	HBG004841.
Family and domain databases
InterPro	IPR008979. Galactose-bd-like. IPR019801. Glyco_hydro_35_CS. IPR013781. Glyco_hydro_subgr_catalytic. IPR001944. Glycoside_Hdrlase_35. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
PANTHER	PTHR23421. Glyco_hydro_35. 1 hit.
Pfam	PF01301. Glyco_hydro_35. 1 hit. [Graphical view]
PRINTS	PR00742. GLHYDRLASE35.
SUPFAM	SSF49785. Gal_bind_like. 1 hit. SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS01182. GLYCOSYL_HYDROL_F35. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BGAL_FELCA

Accession

Primary (citable) accession number: O19015
Secondary accession number(s): O18898

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	January 1, 1998
Last modified:	June 28, 2011
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program