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Protein

Serine/threonine-protein kinase A-Raf

Gene

ARAF

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May also regulate the TOR signaling cascade (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi99Zinc 1By similarity1
Metal bindingi112Zinc 2By similarity1
Metal bindingi115Zinc 2By similarity1
Metal bindingi125Zinc 1By similarity1
Metal bindingi128Zinc 1By similarity1
Metal bindingi133Zinc 2By similarity1
Metal bindingi136Zinc 2By similarity1
Metal bindingi144Zinc 1By similarity1
Binding sitei336ATPPROSITE-ProRule annotation1
Active sitei429Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri98 – 144Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST47
Nucleotide bindingi316 – 324ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.10.2. 6170.
ReactomeiR-SSC-5673000. RAF activation.
R-SSC-5675221. Negative regulation of MAPK pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase A-Raf (EC:2.7.11.1)
Alternative name(s):
Proto-oncogene A-Raf
Proto-oncogene A-Raf-1
Gene namesi
Name:ARAF
Synonyms:ARAF1
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Chromosome X

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000856241 – 606Serine/threonine-protein kinase A-RafAdd BLAST606

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei157PhosphoserineBy similarity1
Modified residuei162PhosphoserineBy similarity1
Modified residuei181PhosphothreonineBy similarity1
Modified residuei186PhosphoserineBy similarity1
Modified residuei257PhosphoserineBy similarity1
Modified residuei269PhosphoserineBy similarity1
Modified residuei318PhosphothreonineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO19004.
PRIDEiO19004.

Expressioni

Gene expression databases

BgeeiENSSSCG00000012275.
GenevisibleiO19004. SS.

Interactioni

Subunit structurei

Interacts with TH1L/NELFD.By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000013063.

Structurei

3D structure databases

ProteinModelPortaliO19004.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 91RBDPROSITE-ProRule annotationAdd BLAST73
Domaini310 – 570Protein kinasePROSITE-ProRule annotationAdd BLAST261

Sequence similaritiesi

Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 RBD (Ras-binding) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri98 – 144Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST47

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG0193. Eukaryota.
ENOG410Y4UP. LUCA.
GeneTreeiENSGT00760000118807.
HOGENOMiHOG000252972.
HOVERGENiHBG001886.
InParanoidiO19004.
KOiK08845.
OMAiGAQQVEQ.
OrthoDBiEOG091G09SB.
TreeFamiTF317006.

Family and domain databases

CDDicd00029. C1. 1 hit.
InterProiIPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR003116. RBD_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF02196. RBD. 1 hit.
[Graphical view]
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00455. RBD. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50898. RBD. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O19004-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPPRGPPAN GAEPSRAVGT VKVYLPNKQR TVVTVRDGMS VYDSLDKALK
60 70 80 90 100
VRGLNQDCCV VYRLIKGRKT VTAWDTAIAP LDGEELIVEV LEDVPLTMHN
110 120 130 140 150
FVRKTFFSLA FCDFCLKFLF HGFRCQTCGY KFHQHCSSKV PTVCVDMSTN
160 170 180 190 200
RRQFYHSVQD LSGGSRQHET PSNRPLNEPL TPQGPSSCTQ HRDPEHFPFP
210 220 230 240 250
APANAPLQRI RSTSTPNVHM VSTTAPMDSG LVQLTAQSFN TDAAGNRGGG
260 270 280 290 300
DGAPRGSPSP ASVSSGRKSP HSKSPSEQRE RKSLADDKKK VKNLGYRDSG
310 320 330 340 350
YYWEVPPSEV QLLKRIGTGS FGTVFRGRWH GDVAVKVLKV AQPTAEQAQA
360 370 380 390 400
FKNEMQVLRK TRHVNILLFM GFMTRPGFAI ITQWCEGSSL YHHLHVADTR
410 420 430 440 450
FDMVQLIDVA RQTAQGMDYL HAKNIIHRDL KSNNIFLHEG LTVKIGDFGL
460 470 480 490 500
ATVKTRWSGA QPLEQPSGSV LWMAAEVIRM QDPNPYSFQS DVYAYGVVLY
510 520 530 540 550
ELMTGSLPYS HIGSRDQIIF MVGRGYLSPD LSKISSNCPK AMRRLLSDCL
560 570 580 590 600
KFQREERPLF PQILATIELL QRSLPKIERS ASEPSLHRTQ ADELPACLLS

AARLVP
Length:606
Mass (Da):67,538
Last modified:January 1, 1998 - v1
Checksum:i1A7EEB9A5D9DE152
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88385 mRNA. Translation: BAA22379.1.
RefSeqiNP_999494.1. NM_214329.1.
XP_005657855.1. XM_005657798.2.
UniGeneiSsc.2370.

Genome annotation databases

EnsembliENSSSCT00000013423; ENSSSCP00000013063; ENSSSCG00000012275.
ENSSSCT00000034636; ENSSSCP00000030304; ENSSSCG00000012275.
GeneIDi397601.
KEGGissc:397601.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D88385 mRNA. Translation: BAA22379.1.
RefSeqiNP_999494.1. NM_214329.1.
XP_005657855.1. XM_005657798.2.
UniGeneiSsc.2370.

3D structure databases

ProteinModelPortaliO19004.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000013063.

Proteomic databases

PaxDbiO19004.
PRIDEiO19004.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSSSCT00000013423; ENSSSCP00000013063; ENSSSCG00000012275.
ENSSSCT00000034636; ENSSSCP00000030304; ENSSSCG00000012275.
GeneIDi397601.
KEGGissc:397601.

Organism-specific databases

CTDi369.

Phylogenomic databases

eggNOGiKOG0193. Eukaryota.
ENOG410Y4UP. LUCA.
GeneTreeiENSGT00760000118807.
HOGENOMiHOG000252972.
HOVERGENiHBG001886.
InParanoidiO19004.
KOiK08845.
OMAiGAQQVEQ.
OrthoDBiEOG091G09SB.
TreeFamiTF317006.

Enzyme and pathway databases

BRENDAi2.7.10.2. 6170.
ReactomeiR-SSC-5673000. RAF activation.
R-SSC-5675221. Negative regulation of MAPK pathway.

Gene expression databases

BgeeiENSSSCG00000012275.
GenevisibleiO19004. SS.

Family and domain databases

CDDicd00029. C1. 1 hit.
InterProiIPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR003116. RBD_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF02196. RBD. 1 hit.
[Graphical view]
PRINTSiPR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00455. RBD. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50898. RBD. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARAF_PIG
AccessioniPrimary (citable) accession number: O19004
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: November 30, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.