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Reviewed, UniProtKB/Swiss-Prot O19004 (ARAF_PIG)

Last modified June 16, 2009. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    A-Raf proto-oncogene serine/threonine-protein kinase
    EC=2.7.11.1
Alternative name(s):
    A-Raf-1
Gene names
Name: ARAF
Synonyms: ARAF1
OrganismSus scrofa (Pig)
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

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Protein attributes

Sequence length606 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Involved in the transduction of mitogenic signals from the cell membrane to the nucleus By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Binds 2 zinc ions per subunit By similarity.

Subunit structure

Interacts with TH1L/NELFD By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.

Contains 1 phorbol-ester/DAG-type zinc finger.

Contains 1 protein kinase domain.

Contains 1 RBD (Ras-binding) domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 606606A-Raf proto-oncogene serine/threonine-protein kinase
PRO_0000085624

Regions

Domain19 – 9173RBD
Domain310 – 570261Protein kinase
Zinc finger98 – 14447Phorbol-ester/DAG-type
Nucleotide binding316 – 3249ATP By similarity

Sites

Active site4291Proton acceptor By similarity
Metal binding991Zinc 1 By similarity
Metal binding1121Zinc 2 By similarity
Metal binding1151Zinc 2 By similarity
Metal binding1251Zinc 1 By similarity
Metal binding1281Zinc 1 By similarity
Metal binding1331Zinc 2 By similarity
Metal binding1361Zinc 2 By similarity
Metal binding1441Zinc 1 By similarity
Binding site3361ATP By similarity

Amino acid modifications

Modified residue1861Phosphoserine By similarity
Modified residue2131Phosphothreonine By similarity
Modified residue2151Phosphothreonine By similarity
Modified residue2571Phosphoserine By similarity
Modified residue5801Phosphoserine By similarity
Modified residue5821Phosphoserine By similarity

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Sequences

Sequence LengthMass (Da)Tools
O19004-1 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 1A7EEB9A5D9DE152

FASTA60667,538
        10         20         30         40         50         60 
MEPPRGPPAN GAEPSRAVGT VKVYLPNKQR TVVTVRDGMS VYDSLDKALK VRGLNQDCCV 

        70         80         90        100        110        120 
VYRLIKGRKT VTAWDTAIAP LDGEELIVEV LEDVPLTMHN FVRKTFFSLA FCDFCLKFLF 

       130        140        150        160        170        180 
HGFRCQTCGY KFHQHCSSKV PTVCVDMSTN RRQFYHSVQD LSGGSRQHET PSNRPLNEPL 

       190        200        210        220        230        240 
TPQGPSSCTQ HRDPEHFPFP APANAPLQRI RSTSTPNVHM VSTTAPMDSG LVQLTAQSFN 

       250        260        270        280        290        300 
TDAAGNRGGG DGAPRGSPSP ASVSSGRKSP HSKSPSEQRE RKSLADDKKK VKNLGYRDSG 

       310        320        330        340        350        360 
YYWEVPPSEV QLLKRIGTGS FGTVFRGRWH GDVAVKVLKV AQPTAEQAQA FKNEMQVLRK 

       370        380        390        400        410        420 
TRHVNILLFM GFMTRPGFAI ITQWCEGSSL YHHLHVADTR FDMVQLIDVA RQTAQGMDYL 

       430        440        450        460        470        480 
HAKNIIHRDL KSNNIFLHEG LTVKIGDFGL ATVKTRWSGA QPLEQPSGSV LWMAAEVIRM 

       490        500        510        520        530        540 
QDPNPYSFQS DVYAYGVVLY ELMTGSLPYS HIGSRDQIIF MVGRGYLSPD LSKISSNCPK 

       550        560        570        580        590        600 
AMRRLLSDCL KFQREERPLF PQILATIELL QRSLPKIERS ASEPSLHRTQ ADELPACLLS 


AARLVP

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References

[1]"Assignment of ARAF1 to porcine chromosome Xp11.2-p13 by fluorescence in situ hybridization."
Yasue H., Adams L., Ozawa A., Hanazono M., Li N., Lin Z.H., Kusumoto H.
Mamm. Genome 8:457-458(1997) [PubMed: 9166601] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Landrace.
Tissue: Liver.

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Cross-references

Sequence databases

D88385 mRNA. Translation: BAA22379.1.
RefSeqNP_999494.1.
UniGeneSsc.2370

3D structure databases

HSSPHSSP built from PDB template 1FAR based on UniProtKB P04049.
SMRO19004. Positions 20-91, 96-146, 303-576.
ModBaseSearch...

Genome annotation databases

GeneID397601.
KEGGssc:397601.

Phylogenomic databases

HOVERGENO19004.

Enzyme and pathway databases

BRENDA2.7.10.2. 249.
2.7.11.1. 249.

Family and domain databases

InterProIPR002219. DAG_PE_bd.
IPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR003116. Raf-like_ras_bd.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
[Graphical view]
PfamPF00130. C1_1. 1 hit.
PF00069. Pkinase. 1 hit.
PF02196. RBD. 1 hit.
[Graphical view]
ProDomPD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00109. C1. 1 hit.
SM00455. RBD. 1 hit.
[Graphical view]
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50898. RBD. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameARAF_PIG
AccessionPrimary (citable) accession number: O19004
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: January 1, 1998
Last modified: June 16, 2009
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents