Reviewed, UniProtKB/Swiss-Prot O18994 (GPX5_PIG)

Names and origin

Protein names

Recommended name:
    Epididymal secretory glutathione peroxidase
    EC=1.11.1.9
Alternative name(s):
    Epididymis-specific glutathione peroxidase-like protein
      Short name=EGLP
    Glutathione peroxidase 5
      Short name=GSHPx-5
      Short name=GPx-5

Gene names

Name:

GPX5

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May constitute a glutathione peroxidase-like protective system against peroxide damage in sperm membrane lipids. Since the purified porcine enzyme has very little activity towards hydrogen peroxide or organic hydroperoxides the protective effect is not likely to be exerted by its enzymatic activity. Instead, may protect sperm from premature acrosome reaction in the epididymis by binding to lipid peroxides, which might otherwise interact with phospholipase A2 and induce the acrosome reaction.
Catalytic activity	2 glutathione + H2O2 = glutathione disulfide + 2 H2O.
Subunit structure	Homotetramer.
Subcellular location	Secreted.
Tissue specificity	Proximal caput epididymis.
Sequence similarities	Belongs to the glutathione peroxidase family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Oxidoreductase Peroxidase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to oxidative stress Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutathione peroxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Ref.1
	Chain	22 – 219	198	Epididymal secretory glutathione peroxidase		PRO_0000013079
Sites
	Active site	71	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	O18994-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: A22850A6477A262D	FASTA	219	24,936
	10 20 30 40 50 60 MTVQLGAFYL FPLFMAGFVQ TNSNLEKMDC YKDVTGTIYD YDAFTLNGNE HIQFKQYAGK 70 80 90 100 110 120 HVLFVNVATY CGLTAQYPEL NTLQEELKPF GLVVLGFPCN QFGKQEPGEN SEILLGLKYV 130 140 150 160 170 180 RPGGGYVPNF QLFEKGDVNG EKEQKVFTFL KHSCPHPSEL IGSIGYISWE PIRVHDIRWN 190 200 210 FEKFLVGPDG VPVMRWVHET PISTVKSDIL AYLKQFKTE

References

[1]

"Molecular cloning and characterization of the epididymis-specific glutathione peroxidase-like protein secreted in the porcine epididymal fluid." Okamura N., Iwaki Y., Hiramoto S., Tamba M., Bannai S., Sugita Y., Syntin P., Dacheux F., Dacheux J.-L. Biochim. Biophys. Acta 1336:99-109(1997) [PubMed: 9271255] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-46, FUNCTION. Tissue: Epididymis.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D37916 mRNA. Translation: BAA22149.1.
RefSeq	NP_999051.1.
UniGene	Ssc.14513
3D structure databases
SMR	O18994. Positions 29-216.
ModBase	Search...
Protein family/group databases
PeroxiBase	3724. SscGPx05.
Genome annotation databases
Ensembl	ENSSSCT00000001311; ENSSSCP00000001282; ENSSSCG00000001213; Sus scrofa. [Genome view] ENSSSCT00000001312; ENSSSCP00000001283; ENSSSCG00000001214; Sus scrofa. [Genome view]
GeneID	396920.
KEGG	ssc:396920.
Organism-specific databases
CTD	396920.
Phylogenomic databases
HOVERGEN	O18994.
Enzyme and pathway databases
BRENDA	1.11.1.9. 249.
Family and domain databases
InterPro	IPR000889. Glutathione_peroxidase. IPR012336. Thioredoxin-like_fold. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR11592. Glut_peroxidase. 1 hit.
Pfam	PF00255. GSHPx. 1 hit. [Graphical view]
PIRSF	PIRSF000303. Glutathion_perox. 1 hit.
PRINTS	PR01011. GLUTPROXDASE.
PROSITE	PS00460. GLUTATHIONE_PEROXID_1. 1 hit. PS00763. GLUTATHIONE_PEROXID_2. 1 hit. PS51355. GLUTATHIONE_PEROXID_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPX5_PIG

Accession

Primary (citable) accession number: O18994

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 1, 1998
Last modified:	February 9, 2010
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)