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O18994 (GPX5_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Epididymal secretory glutathione peroxidase
EC=1.11.1.9
Alternative name(s):
Epididymis-specific glutathione peroxidase-like protein
Short name=EGLP
Glutathione peroxidase 5
Short name=GPx-5
Short name=GSHPx-5
Gene names
Name:GPX5
OrganismSus scrofa (Pig) [Complete proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length219 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May constitute a glutathione peroxidase-like protective system against peroxide damage in sperm membrane lipids. Since the purified porcine enzyme has very little activity towards hydrogen peroxide or organic hydroperoxides the protective effect is not likely to be exerted by its enzymatic activity. Instead, may protect sperm from premature acrosome reaction in the epididymis by binding to lipid peroxides, which might otherwise interact with phospholipase A2 and induce the acrosome reaction. Ref.1

Catalytic activity

2 glutathione + H2O2 = glutathione disulfide + 2 H2O.

Subunit structure

Homotetramer.

Subcellular location

Secreted.

Tissue specificity

Proximal caput epididymis.

Sequence similarities

Belongs to the glutathione peroxidase family.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   Molecular functionOxidoreductase
Peroxidase
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processresponse to oxidative stress

Inferred from electronic annotation. Source: InterPro

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionglutathione peroxidase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Ref.1
Chain22 – 219198Epididymal secretory glutathione peroxidase
PRO_0000013079

Sites

Active site711 By similarity

Sequences

Sequence LengthMass (Da)Tools
O18994 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: A22850A6477A262D

FASTA21924,936
        10         20         30         40         50         60 
MTVQLGAFYL FPLFMAGFVQ TNSNLEKMDC YKDVTGTIYD YDAFTLNGNE HIQFKQYAGK 

        70         80         90        100        110        120 
HVLFVNVATY CGLTAQYPEL NTLQEELKPF GLVVLGFPCN QFGKQEPGEN SEILLGLKYV 

       130        140        150        160        170        180 
RPGGGYVPNF QLFEKGDVNG EKEQKVFTFL KHSCPHPSEL IGSIGYISWE PIRVHDIRWN 

       190        200        210 
FEKFLVGPDG VPVMRWVHET PISTVKSDIL AYLKQFKTE

« Hide

References

[1]"Molecular cloning and characterization of the epididymis-specific glutathione peroxidase-like protein secreted in the porcine epididymal fluid."
Okamura N., Iwaki Y., Hiramoto S., Tamba M., Bannai S., Sugita Y., Syntin P., Dacheux F., Dacheux J.-L.
Biochim. Biophys. Acta 1336:99-109(1997) [PubMed: 9271255] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-46, FUNCTION.
Tissue: Epididymis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D37916 mRNA. Translation: BAA22149.1.
RefSeqNP_999051.1. NM_213886.1.
UniGeneSsc.14513.

3D structure databases

ProteinModelPortalO18994.
SMRO18994. Positions 29-216.
ModBaseSearch...

Protein-protein interaction databases

STRINGO18994.

Protein family/group databases

PeroxiBase3724. SscGPx05.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSSSCT00000001311; ENSSSCP00000001282; ENSSSCG00000001213.
ENSSSCT00000001312; ENSSSCP00000001283; ENSSSCG00000001214.
GeneID396920.
KEGGssc:396920.

Organism-specific databases

CTD2880.

Phylogenomic databases

GeneTreeENSGT00550000074312.
HOVERGENHBG004333.
OMAEPGDNKE.
OrthoDBEOG4CRM10.

Family and domain databases

InterProIPR000889. Glutathione_peroxidase.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
Gene3DG3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
KOK00432.
PANTHERPTHR11592. Glut_peroxidase. 1 hit.
PfamPF00255. GSHPx. 1 hit.
[Graphical view]
PIRSFPIRSF000303. Glutathion_perox. 1 hit.
PRINTSPR01011. GLUTPROXDASE.
SUPFAMSSF52833. Thiordxn-like_fd. 1 hit.
PROSITEPS00460. GLUTATHIONE_PEROXID_1. 1 hit.
PS00763. GLUTATHIONE_PEROXID_2. 1 hit.
PS51355. GLUTATHIONE_PEROXID_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGPX5_PIG
AccessionPrimary (citable) accession number: O18994
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: November 16, 2011
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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