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Protein

Cytochrome P450 2E1

Gene

CYP2E1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites.By similarity

Catalytic activityi

4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi437 – 4371Iron (heme axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2E1 (EC:1.14.13.-)
Alternative name(s):
4-nitrophenol 2-hydroxylase (EC:1.14.13.n7)
CYPIIE1
Gene namesi
Name:CYP2E1
Synonyms:CYP2E
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 495494Cytochrome P450 2E1PRO_0000051750Add
BLAST

Proteomic databases

PaxDbiO18963.
PeptideAtlasiO18963.
PRIDEiO18963.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000024437.

Structurei

3D structure databases

ProteinModelPortaliO18963.
SMRiO18963. Positions 33-493.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni298 – 3036Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiO18963.
KOiK07415.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008070. Cyt_P450_E_grp-I_CYP2E-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01687. EP450ICYP2E.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O18963-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAALGITVAL LVWMATLLFI SIWKHIYSSW KLPPGPFPLP IIGNLLQLDI
60 70 80 90 100
KNIPKSFTRL AERYGPVFTL YLGSQRAVVV HGYKPVKEVL LDYKNEFSGR
110 120 130 140 150
GENPGFQMHK NNGIIFNNGS TWRDTRRFSL TTLRDLGMGK QGNEQRIQRE
160 170 180 190 200
AHFLLEVLRK TQGQPFDPTF VVGFAPYNVI SDILFHKRFD YKDQTSLRLM
210 220 230 240 250
SLFNENFYLL SSPWIQLYNN FPDYLQYLPG SHRKLLKNVS EVKSYALERV
260 270 280 290 300
KDHQKSLEPS CPRGFLDTML IEMAKERHSV DPMYTLENIA VTVADLLFAG
310 320 330 340 350
TETTSTTLRY GLLILMKYPE VEEKLHEEID RVIGPSRIPA VKDRLDMPYL
360 370 380 390 400
DAVVHEIQRF IDLLPSNLLH EATQDTVFRG YVIPKGTVVI PTLDSVLHDR
410 420 430 440 450
QEFPEPEKFK PEHFLNENGK FKYSDHFKAF SAGKRVCVGE GLARMELFLL
460 470 480 490
LAAILQHFNL KSLVDPKDID LSPIAIGFGK IPPRYKLCLI PRSKV
Length:495
Mass (Da):56,827
Last modified:January 1, 1998 - v1
Checksum:i0055EB5EB6FCE1AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001715 mRNA. Translation: CAA04948.1.
DQ058608 mRNA. Translation: AAY83882.1.
DQ058605 Genomic DNA. Translation: AAY83880.1.
RefSeqiNP_776955.2. NM_174530.3.
UniGeneiBt.5532.

Genome annotation databases

GeneIDi282213.
KEGGibta:282213.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001715 mRNA. Translation: CAA04948.1.
DQ058608 mRNA. Translation: AAY83882.1.
DQ058605 Genomic DNA. Translation: AAY83880.1.
RefSeqiNP_776955.2. NM_174530.3.
UniGeneiBt.5532.

3D structure databases

ProteinModelPortaliO18963.
SMRiO18963. Positions 33-493.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000024437.

Proteomic databases

PaxDbiO18963.
PeptideAtlasiO18963.
PRIDEiO18963.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi282213.
KEGGibta:282213.

Organism-specific databases

CTDi1571.

Phylogenomic databases

eggNOGiKOG0156. Eukaryota.
COG2124. LUCA.
HOGENOMiHOG000036992.
HOVERGENiHBG015789.
InParanoidiO18963.
KOiK07415.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008070. Cyt_P450_E_grp-I_CYP2E-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01687. EP450ICYP2E.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation of a full length cytochrome P450 (CYP2E) cDNA sequence and its functional expression in V79 cells."
    van Raak M., Natsuhori M., Ligtenberg M., Kleij L., ten Berghe D., de Groene E.M., van Miert A.S., Witkamp R.F., Horbach G.J.
    Submitted (OCT-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Hereford.
    Tissue: Liver.
  2. "Comparative mapping of the bovine SPRN locus."
    Ferretti L., Uboldi C., Del Vecchio I., Eggen A., Brunner R., Iannuzzi L.
    Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].

Entry informationi

Entry nameiCP2E1_BOVIN
AccessioniPrimary (citable) accession number: O18963
Secondary accession number(s): Q4PS78
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: July 6, 2016
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.