O18956 (ENTP1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ectonucleoside triphosphate diphosphohydrolase 1
Short name=NTPDase 1
EC=3.6.1.5

Alternative name(s):
Ecto-ATP diphosphohydrolase 1
Short name=Ecto-ATPDase 1
Short name=Ecto-ATPase 1
Ecto-apyrase
Lymphoid cell activation antigen
CD_antigen=CD39

Gene names

Name:	ENTPD1
Synonyms:	CD39

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	513 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation. Hydrolyzes ATP and ADP equally well.
Catalytic activity	ATP + 2 H2O = AMP + 2 phosphate.
Cofactor	Calcium. Magnesium.
Subunit structure	Homodimer; disulfide-linked By similarity.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the GDA1/CD39 NTPase family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Calcium Magnesium Nucleotide-binding
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 513	513	Ectonucleoside triphosphate diphosphohydrolase 1		PRO_0000209901
Regions
	Topological domain	1 – 16	16	Cytoplasmic Potential
	Transmembrane	17 – 37	21	Helical; Potential
	Topological domain	38 – 481	444	Extracellular Potential
	Transmembrane	482 – 502	21	Helical; Potential
	Topological domain	503 – 513	11	Cytoplasmic Potential
Sites
	Active site	174	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	65	1	Phosphotyrosine By similarity
	Glycosylation	73	1	N-linked (GlcNAc...) Potential
	Glycosylation	227	1	N-linked (GlcNAc...) Potential
	Glycosylation	245	1	N-linked (GlcNAc...) Potential
	Glycosylation	307	1	N-linked (GlcNAc...) Potential
	Glycosylation	336	1	N-linked (GlcNAc...) Potential
	Glycosylation	373	1	N-linked (GlcNAc...) Potential
	Glycosylation	460	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	97	1	K → N AA sequence Ref.2
	Sequence conflict	101 – 103	3	INV → CGF AA sequence Ref.2
	Sequence conflict	464	1	K → V AA sequence Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O18956 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 20FE98F27B6D2F96	FASTA	513	58,114
	10 20 30 40 50 60 MEDRRESELK VFCSKNILSI LGFSCIIAVI ALLALGLTQN KALPENVKFG IVLDAGSSHT 70 80 90 100 110 120 SLYIYRWPAE KENDTGVVTQ IEESNVKGPG ISGFAKKVNE INVYLTACME RAQKVIPSIQ 130 140 150 160 170 180 HMETPVYLGA TAGMRLLRME NKQMADKILA AVASSISEYP FDFQGARIIS GQEEGAYGWI 190 200 210 220 230 240 TVNYLLGKFT QKLSWFNLKP SKDDTQETYG ALDLGGASTQ ITFVPQNETT ESPNNNLYFR 250 260 270 280 290 300 LYGKNYSVYT HSFLCYGKDQ ALLQKLALGL QGTNGIIHEP CFHSRYMRKI KMSVLNEGFC 310 320 330 340 350 360 TKRHELNSSF YPLVDIEIRG AGNFQRCRQS IIQLFNTSYC PYSSCSFNGV FLPPLHGQFG 370 380 390 400 410 420 AFSAFYYVME FLNLTSEESV SVEQLTEKLE EFCAQRWEEV QKNFGEVKEK YLSEYCFSGT 430 440 450 460 470 480 YILVLLLNGY HFTAESWKNI HFMNKVRSTD VGWTLGYMLN LTNKIPAEEP MSPPLPHSTY 490 500 510 VFLMVLFSLI LLAVIIVGIV VFHKPSYFWK DMV

References

[1]	Chang A.S., Garcia R.L., Chang S.M., Schilling W.P. Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Aortic endothelium.
[2]	"Identification and characterization of CD39/vascular ATP diphosphohydrolase." Kaczmarek E., Koziak K., Sevigny J., Siegel J.B., Anrather J., Beaudoin A.R., Bach F.H., Robson S.C. J. Biol. Chem. 271:33116-33122(1996) [PubMed: 8955160] [Abstract] Cited for: PROTEIN SEQUENCE OF 97-103; 123-133; 136-140; 145-168 AND 459-471. Tissue: Aorta.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF005940 mRNA. Translation: AAB62382.1.
IPI	IPI00839599.
RefSeq	NP_776961.1. NM_174536.2.
UniGene	Bt.4117.
3D structure databases
ProteinModelPortal	O18956.
ModBase	Search...
Protein-protein interaction databases
STRING	O18956.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	282223.
KEGG	bta:282223.
Organism-specific databases
CTD	953.
Phylogenomic databases
GeneTree	ENSGT00550000074435.
HOVERGEN	HBG018982.
OrthoDB	EOG48WC1R.
PhylomeDB	O18956.
Family and domain databases
InterPro	IPR000407. GDA1_CD39_NTPase. [Graphical view]
KO	K01510.
PANTHER	PTHR11782. GDA1_CD39_NTPase. 1 hit.
Pfam	PF01150. GDA1_CD39. 1 hit. [Graphical view]
PROSITE	PS01238. GDA1_CD39_NTPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ENTP1_BOVIN

Accession

Primary (citable) accession number: O18956

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	January 1, 1998
Last modified:	November 16, 2011
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program