Reviewed, UniProtKB/Swiss-Prot O18823 (AOAH_RABIT)

Names and origin

Protein names

Recommended name:
    Acyloxyacyl hydrolase
    EC=3.1.1.77
Cleaved into the following 2 chains:
    1- Recommended name:
            Acyloxyacyl hydrolase small subunit
    2- Recommended name:
            Acyloxyacyl hydrolase large subunit

Gene names

Name:

AOAH

Organism

Oryctolagus cuniculus (Rabbit)

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	575 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Removes the secondary (acyloxyacyl-linked) fatty acyl chains from the lipid A region of bacterial lipopolysaccharides By similarity.
Catalytic activity	3-(acyloxy)acyl group of bacterial toxin = 3-hydroxyacyl group of bacterial toxin + a fatty acid.
Subunit structure	Heterodimer By similarity.
Post-translational modification	Both subunits contain a number of cysteine residues that may form disulfide bridges By similarity.
Sequence similarities	Contains 1 saposin B-type domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	lipid metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	acyloxyacyl hydrolase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Propeptide	26 – 34	9	By similarity		PRO_0000041815
	Chain	35 – 156	122	Acyloxyacyl hydrolase small subunit		PRO_0000041816
	Chain	157 – 575	419	Acyloxyacyl hydrolase large subunit		PRO_0000041817
Regions
	Domain	36 – 117	82	Saposin B-type
Sites
	Active site	262	1	Potential
Amino acid modifications
	Glycosylation	58	1	N-linked (GlcNAc...) Potential
	Glycosylation	206	1	N-linked (GlcNAc...) Potential
	Glycosylation	335	1	N-linked (GlcNAc...) Potential
	Glycosylation	466	1	N-linked (GlcNAc...) Potential
	Disulfide bond	40 ↔ 113		By similarity
	Disulfide bond	43 ↔ 107		By similarity
	Disulfide bond	69 ↔ 82		By similarity

Sequences

	Sequence		Length	Mass (Da)
	O18823-1 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: 5AB2CDADE568492B	FASTA	575	65,053
	10 20 30 40 50 60 MKSPWRILVV SPLLLLPLHS STSRAHDNQP GTIRSDHYTC VGCVLVVSVI EQLAQVHNST 70 80 90 100 110 120 VQASMERLCS YLPEEWVLKT ACYMMVHVFG ADIIKLFDKD VNADVVCHTL EFCKQEPGQP 130 140 150 160 170 180 LCHLYPLPKE SWKFTLEKAR HIVKQSPIMK YTRSGAGICS LPFLAKICQK IKLAIKNSVP 190 200 210 220 230 240 IKDVDSDKYS IFPTLRGYHW RGRDCNDSDK TVYPGRRPDN WDAHRDSNCN GIWGVDPKDG 250 260 270 280 290 300 IPYEKKFCEG SQPRGIILLG DSAGAHFHIP PEWLTVSQMS VNSFLNLPTA VTNELDWPQL 310 320 330 340 350 360 SGTTGFLDSA SKIKENSIYL RLRKRNRCNH RDYQNISKNG ASSRNVKSLI ESLSRNQLLD 370 380 390 400 410 420 HPAIVIYAMI GNDVCNGRKT DPVSAMTTPE QLYANVLKML EALNSHLPTG SHVILYGLAH 430 440 450 460 470 480 GAFLWDTLHS RYHPLGQLNK DVTYTQLYSF LGCLQVSPCP GWMSANETLR ALTSERAQQL 490 500 510 520 530 540 SETLRKIAAS KKFTNFNLFY LDFAFQEVVE EWQKMGGQPW ELIEAVDGFH PNEVALLLFA 550 560 570 DQLWEKVQRQ WPDVLGKENP FNPQIEEVFG DQGGH

References

[1]	"Human, murine, and lapine acyloxyacyl hydrolases share unique structural features." Munford R.S., Fosmire S., Varley A.W., Staab J.F. Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: New Zealand white.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF018173 mRNA. Translation: AAB81183.1.
RefSeq	NP_001075494.1.
UniGene	Ocu.2181
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	100008662.
Organism-specific databases
CTD	100008662.
Phylogenomic databases
HOVERGEN	O18823.
Enzyme and pathway databases
BRENDA	3.1.1.77. 255.
Family and domain databases
InterPro	IPR001087. Lipase_GDSL. IPR008265. Lipase_GDSL_AS. IPR008138. SapB_2. IPR011001. Saposin-like. IPR008139. SaposinB. [Graphical view]
Gene3D	G3DSA:1.10.225.10. Saposin_like. 1 hit.
Pfam	PF00657. Lipase_GDSL. 1 hit. PF03489. SapB_2. 1 hit. [Graphical view]
SMART	SM00741. SapB. 1 hit. [Graphical view]
PROSITE	PS01098. LIPASE_GDSL_SER. False negative. PS50015. SAP_B. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AOAH_RABIT

Accession

Primary (citable) accession number: O18823

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	January 1, 1998
Last modified:	September 1, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)