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O18750 (ENPL_RABIT) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Endoplasmin
Alternative name(s):
94 kDa glucose-regulated protein
Short name=GRP-94
Heat shock protein 90 kDa beta member 1
Gene names
Name:HSP90B1
Synonyms:GRP94
OrganismOryctolagus cuniculus (Rabbit) [Reference proteome]
Taxonomic identifier9986 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus

Protein attributes

Sequence length716 AA.
Sequence statusFragment.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity By similarity. HAMAP-Rule MF_00505

Subunit structure

Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5 By similarity. Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9 By similarity. Interacts with CNPY3; this interaction is disrupted in the presence of ATP. Interacts with several TLRs, including TLR4 and TLR9, but not with TLR3 By similarity. Interacts with MZB1 in a calcium-dependent manner By similarity. Interacts with METTL23 By similarity.

Subcellular location

Endoplasmic reticulum lumen. Melanosome By similarity HAMAP-Rule MF_00505.

Post-translational modification

Phosphorylated By similarity. HAMAP-Rule MF_00505

Sequence similarities

Belongs to the heat shock protein 90 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 716›716Endoplasmin HAMAP-Rule MF_00505
PRO_0000062927

Regions

Motif713 – 7164Prevents secretion from ER Potential

Sites

Binding site281ATP By similarity
Binding site701ATP By similarity
Binding site831ATP By similarity
Binding site891ATP By similarity
Binding site1201ATP; via amide nitrogen By similarity
Binding site3681ATP By similarity

Amino acid modifications

Modified residue3241N6-succinyllysine By similarity
Modified residue3991N6-acetyllysine By similarity
Modified residue5521N6-succinyllysine By similarity
Glycosylation281N-linked (GlcNAc...) Potential
Glycosylation1381N-linked (GlcNAc...) Potential
Glycosylation3651N-linked (GlcNAc...) Potential
Glycosylation4211N-linked (GlcNAc...) Potential
Disulfide bond59Interchain By similarity

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
O18750 [UniParc].

Last modified January 1, 1998. Version 1.
Checksum: 7098C6442F0EC84B

FASTA71682,608
        10         20         30         40         50         60 
AEVNRMMKLI INSLYKNKEI FLRELISNAS DALDKIRLIS LTDEQALSGN EELTVKIKCD 

        70         80         90        100        110        120 
KEKNLLHVTD TGVGMTREEL VKNLGTIAKS GTSEFLNKMT EAQEDGQSTS ELIGQFGVGF 

       130        140        150        160        170        180 
YSAFLVADKV IVTSKHNNDT QHIWESDSNE FSVIADPRGN TLGRGTTITL VLKEEASDYL 

       190        200        210        220        230        240 
ELDTIKNLVK KYSQFINFPI YVWSSKTETV EEPAGEEEAA KEEKEEAVDE AAVEEEEEEK 

       250        260        270        280        290        300 
KPKTKKVEKQ VWDWELMNDI KPIWQRPSKE VEEDEYKAFY KSFSKESDDP MAYIHFTAEE 

       310        320        330        340        350        360 
STFKSILFVP TSAPRGLFDE YGSKKSDYIK LYVRRVFITD DFHDMMPKYL NFVKGVVDSD 

       370        380        390        400        410        420 
DLPLNVSRET LQQHKLLKVI RKKLVRKTLD MIKKIADEKY NDDTFWKGTN IKLGVIEDHS 

       430        440        450        460        470        480 
NRTRLAKLLR FQSSHHPTDI TSLDQYVERM KEKQDKIYFM AGASRKEAES SPFVERLLKK 

       490        500        510        520        530        540 
GYEVIYLTEP VDEYCIQALP EFDGKRFQNV AKEGVKFDES EKTKESREAT EKEFEPLLNW 

       550        560        570        580        590        600 
MKDKALKDKI EKAVVSQRLT ESPCALVASQ YGWSANMERI MKAQAYQTGK DSTKYYASQK 

       610        620        630        640        650        660 
TFEINPRHPL IRDMLRIKED DKTVMDLAVV LFETAILRSG YLLPDTKAYG DRIERIVRLS 

       670        680        690        700        710 
LNIDPDAKVE EEPEEEPEDT TEDTEQDEEE EMDAGTDEQE QEQEPEKKST AEKDEL 

« Hide

References

[1]"Rabbit cardiac and skeletal myocytes differ in constitutive and inducible expression of the glucose-regulated protein GRP94."
Vitadello M., Colpo P., Gorza L.
Biochem. J. 332:351-359(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: New Zealand white.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF001631 mRNA. Translation: AAC48853.1.
UniGeneOcu.4906.

3D structure databases

ProteinModelPortalO18750.
SMRO18750. Positions 1-669.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9986.ENSOCUP00000014008.

Proteomic databases

PRIDEO18750.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG0326.
HOVERGENHBG007374.

Family and domain databases

Gene3D3.30.565.10. 2 hits.
HAMAPMF_00505. HSP90.
InterProIPR015566. Endoplasmin.
IPR003594. HATPase_ATP-bd.
IPR019805. Heat_shock_protein_90_CS.
IPR001404. Hsp90_fam.
IPR020575. Hsp90_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view]
PANTHERPTHR11528. PTHR11528. 1 hit.
PTHR11528:SF21. PTHR11528:SF21. 1 hit.
PfamPF00183. HSP90. 1 hit.
[Graphical view]
PIRSFPIRSF002583. Hsp90. 1 hit.
PRINTSPR00775. HEATSHOCK90.
SMARTSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 2 hits.
PROSITEPS00014. ER_TARGET. 1 hit.
PS00298. HSP90. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameENPL_RABIT
AccessionPrimary (citable) accession number: O18750
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 1, 1998
Last modified: March 19, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families