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Protein

Connective tissue growth factor

Gene

CTGF

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes (By similarity). Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells (By similarity). Enhances fibroblast growth factor-induced DNA synthesis (By similarity).By similarity

GO - Molecular functioni

  1. heparin binding Source: GO_Central
  2. integrin binding Source: GO_Central

GO - Biological processi

  1. angiogenesis Source: Ensembl
  2. cartilage condensation Source: Ensembl
  3. cell adhesion Source: GO_Central
  4. cell-cell signaling Source: GO_Central
  5. cell differentiation Source: Ensembl
  6. cell-matrix adhesion Source: Ensembl
  7. cell migration Source: Ensembl
  8. chondrocyte proliferation Source: Ensembl
  9. DNA biosynthetic process Source: UniProtKB-KW
  10. fibroblast growth factor receptor signaling pathway Source: Ensembl
  11. integrin-mediated signaling pathway Source: Ensembl
  12. lung development Source: Ensembl
  13. negative regulation of cell death Source: GO_Central
  14. negative regulation of gene expression Source: Ensembl
  15. ossification Source: Ensembl
  16. positive regulation of cell differentiation Source: Ensembl
  17. positive regulation of ERK1 and ERK2 cascade Source: Ensembl
  18. positive regulation of JNK cascade Source: Ensembl
  19. positive regulation of stress fiber assembly Source: Ensembl
  20. reactive oxygen species metabolic process Source: Ensembl
  21. regulation of cell growth Source: InterPro
  22. regulation of chondrocyte differentiation Source: Ensembl
  23. signal transduction Source: GO_Central
  24. tissue homeostasis Source: Ensembl
Complete GO annotation...

Keywords - Biological processi

Cell adhesion, DNA synthesis

Keywords - Ligandi

Heparin-binding

Enzyme and pathway databases

ReactomeiREACT_207237. PPARA activates gene expression.
REACT_223100. YAP1- and WWTR1 (TAZ)-stimulated gene expression.

Names & Taxonomyi

Protein namesi
Recommended name:
Connective tissue growth factor
Alternative name(s):
CCN family member 2
Gene namesi
Name:CTGF
Synonyms:CCN2
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Chromosome 9

Subcellular locationi

Secretedextracellular spaceextracellular matrix By similarity. Secreted By similarity

GO - Cellular componenti

  1. Golgi apparatus Source: Ensembl
  2. proteinaceous extracellular matrix Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2626Sequence AnalysisAdd
BLAST
Chaini27 – 349323Connective tissue growth factorPRO_0000014401Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi256 ↔ 293By similarity
Disulfide bondi273 ↔ 307By similarity
Disulfide bondi284 ↔ 323By similarity
Disulfide bondi287 ↔ 325By similarity
Disulfide bondi292 ↔ 329By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiO18739.

Expressioni

Gene expression databases

ExpressionAtlasiO18739. baseline and differential.

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO18739.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 9872IGFBP N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini101 – 16767VWFCPROSITE-ProRule annotationAdd
BLAST
Domaini198 – 24346TSP type-1PROSITE-ProRule annotationAdd
BLAST
Domaini256 – 33075CTCKPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni247 – 349103Heparin-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the CCN family.Curated
Contains 1 CTCK (C-terminal cystine knot-like) domain.PROSITE-ProRule annotation
Contains 1 IGFBP N-terminal domain.PROSITE-ProRule annotation
Contains 1 TSP type-1 domain.PROSITE-ProRule annotation
Contains 1 VWFC domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG72934.
GeneTreeiENSGT00760000119225.
HOGENOMiHOG000231462.
HOVERGENiHBG000635.
InParanoidiO18739.
KOiK06827.
OMAiTYRAKFC.
OrthoDBiEOG7N8ZXB.
TreeFamiTF326070.

Family and domain databases

InterProiIPR006207. Cys_knot_C.
IPR006208. Glyco_hormone_CN.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000867. IGFBP-like.
IPR012395. IGFBP_CNN.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000884. Thrombospondin_1_rpt.
IPR001007. VWF_C.
[Graphical view]
PfamiPF00007. Cys_knot. 1 hit.
PF00219. IGFBP. 1 hit.
PF00090. TSP_1. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
PIRSFiPIRSF036495. IGFBP_rP_CNN. 1 hit.
SMARTiSM00041. CT. 1 hit.
SM00121. IB. 1 hit.
SM00209. TSP1. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS01185. CTCK_1. 1 hit.
PS01225. CTCK_2. 1 hit.
PS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS50092. TSP1. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O18739-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSATGLGPVR CAFVLLLALC SRPASSQDCS APCQCPAGPA PRCPAGVSLV
60 70 80 90 100
LDGCGCCRVC AKQLSELCTE RDPCDPHKGL FCDFGSPANR KIGVCTAKDG
110 120 130 140 150
APCVFGGTVY QSGESFQSSC KYQCTCLDGS VGCVPLCSVD VRLPSPDCPF
160 170 180 190 200
PRRVKLPGKC CEEWVCDEPK EHTVVGPALA AYRPEDTFGP DPTMIRANCL
210 220 230 240 250
VQTTEWSACS KTCGMGISTR VTNDNAFCRL EKQSRLCMVR PCEADLEENI
260 270 280 290 300
KKGKKCIRTP KISKPIKFEL SGCTSMKTYR AKFCGVCTDG RCCTPHRTTT
310 320 330 340
LPVEFKCPDG EVMKKSMMFI KTCACHYNCP GDNDIFESLY YRKMYGDMA
Length:349
Mass (Da):37,924
Last modified:February 11, 2002 - v2
Checksum:i5FFC8EE83EFB4F99
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti28 – 281D → DC in AAB66596 (Ref. 1) Curated
Sequence conflicti58 – 581Missing in AAB66596 (Ref. 1) Curated
Sequence conflicti88 – 881A → T in AAB66596 (Ref. 1) Curated
Sequence conflicti103 – 1042CV → YI in AAB66596 (Ref. 1) Curated
Sequence conflicti166 – 1694CDEP → SRDE in AAB66596 (Ref. 1) Curated
Sequence conflicti184 – 1841P → L in AAB66596 (Ref. 1) Curated
Sequence conflicti200 – 2001L → Q in AAB66596 (Ref. 1) Curated
Sequence conflicti209 – 2091C → Y in AAB66596 (Ref. 1) Curated
Sequence conflicti269 – 2691E → Q in AAB66596 (Ref. 1) Curated
Sequence conflicti284 – 2841C → F in AAB66596 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000137 mRNA. Translation: AAB66596.1.
AF309555 Genomic DNA. Translation: AAG30290.1.
BC113279 mRNA. Translation: AAI13280.1.
RefSeqiNP_776455.1. NM_174030.2.
UniGeneiBt.5240.

Genome annotation databases

EnsembliENSBTAT00000008357; ENSBTAP00000008357; ENSBTAG00000006367.
GeneIDi281103.
KEGGibta:281103.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF000137 mRNA. Translation: AAB66596.1.
AF309555 Genomic DNA. Translation: AAG30290.1.
BC113279 mRNA. Translation: AAI13280.1.
RefSeqiNP_776455.1. NM_174030.2.
UniGeneiBt.5240.

3D structure databases

ProteinModelPortaliO18739.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiO18739.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000008357; ENSBTAP00000008357; ENSBTAG00000006367.
GeneIDi281103.
KEGGibta:281103.

Organism-specific databases

CTDi1490.

Phylogenomic databases

eggNOGiNOG72934.
GeneTreeiENSGT00760000119225.
HOGENOMiHOG000231462.
HOVERGENiHBG000635.
InParanoidiO18739.
KOiK06827.
OMAiTYRAKFC.
OrthoDBiEOG7N8ZXB.
TreeFamiTF326070.

Enzyme and pathway databases

ReactomeiREACT_207237. PPARA activates gene expression.
REACT_223100. YAP1- and WWTR1 (TAZ)-stimulated gene expression.

Miscellaneous databases

NextBioi20805175.

Gene expression databases

ExpressionAtlasiO18739. baseline and differential.

Family and domain databases

InterProiIPR006207. Cys_knot_C.
IPR006208. Glyco_hormone_CN.
IPR009030. Growth_fac_rcpt_N_dom.
IPR000867. IGFBP-like.
IPR012395. IGFBP_CNN.
IPR017891. Insulin_GF-bd_Cys-rich_CS.
IPR000884. Thrombospondin_1_rpt.
IPR001007. VWF_C.
[Graphical view]
PfamiPF00007. Cys_knot. 1 hit.
PF00219. IGFBP. 1 hit.
PF00090. TSP_1. 1 hit.
PF00093. VWC. 1 hit.
[Graphical view]
PIRSFiPIRSF036495. IGFBP_rP_CNN. 1 hit.
SMARTiSM00041. CT. 1 hit.
SM00121. IB. 1 hit.
SM00209. TSP1. 1 hit.
SM00214. VWC. 1 hit.
[Graphical view]
SUPFAMiSSF57184. SSF57184. 1 hit.
SSF82895. SSF82895. 1 hit.
PROSITEiPS01185. CTCK_1. 1 hit.
PS01225. CTCK_2. 1 hit.
PS00222. IGFBP_N_1. 1 hit.
PS51323. IGFBP_N_2. 1 hit.
PS50092. TSP1. 1 hit.
PS01208. VWFC_1. 1 hit.
PS50184. VWFC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Bos taurus connective tissue growth factor."
    Liliensiek B., Lin Z., Fotsis T., Schimanski M., Bierhaus A., Kanitz M., Kauffmann G., Schweigerer L., Ziegler R., Nawroth P.P.
    Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Aorta.
  2. "Bovine connective tissue growth factor, organization of the chromosomal gene and demonstration of promoter activity."
    Mathahs M., Schwitters C., Hove M., Rupp S., Erondu N.E.
    Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.
  3. NIH - Mammalian Gene Collection (MGC) project
    Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Hereford.
    Tissue: Uterus.

Entry informationi

Entry nameiCTGF_BOVIN
AccessioniPrimary (citable) accession number: O18739
Secondary accession number(s): Q2HJ71, Q9GL71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: February 11, 2002
Last modified: February 4, 2015
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.