O18739 (CTGF_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Connective tissue growth factor

Alternative name(s):
CCN family member 2

Gene names

Name:	CTGF
Synonyms:	CCN2

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	349 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes By similarity. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells By similarity. Enhances fibroblast growth factor-induced DNA synthesis By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted › extracellular space › extracellular matrix By similarity. Secreted By similarity.
Sequence similarities	Belongs to the CCN family. Contains 1 CTCK (C-terminal cystine knot-like) domain. Contains 1 IGFBP N-terminal domain. Contains 1 TSP type-1 domain. Contains 1 VWFC domain.

Ontologies

Keywords
Biological process	Cell adhesion DNA synthesis
Cellular component	Extracellular matrix Secreted
Domain	Signal
Ligand	Heparin-binding
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW angiogenesis Inferred from electronic annotation. Source: Compara cartilage condensation Inferred from electronic annotation. Source: Compara cell differentiation Inferred from electronic annotation. Source: Compara cell migration Inferred from electronic annotation. Source: Compara cell-matrix adhesion Inferred from electronic annotation. Source: Compara chondrocyte proliferation Inferred from electronic annotation. Source: Compara fibroblast growth factor receptor signaling pathway Inferred from electronic annotation. Source: Compara integrin-mediated signaling pathway Inferred from electronic annotation. Source: Compara lung development Inferred from electronic annotation. Source: Compara negative regulation of gene expression Inferred from electronic annotation. Source: Compara ossification Inferred from electronic annotation. Source: Compara positive regulation of ERK1 and ERK2 cascade Inferred from electronic annotation. Source: Compara positive regulation of JNK cascade Inferred from electronic annotation. Source: Compara positive regulation of cell differentiation Inferred from electronic annotation. Source: Compara positive regulation of stress fiber assembly Inferred from electronic annotation. Source: Compara reactive oxygen species metabolic process Inferred from electronic annotation. Source: Compara regulation of cell growth Inferred from electronic annotation. Source: InterPro regulation of chondrocyte differentiation Inferred from electronic annotation. Source: Compara
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: Compara proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	heparin binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 349	323	Connective tissue growth factor		PRO_0000014401
Regions
	Domain	27 – 98	72	IGFBP N-terminal
	Domain	101 – 167	67	VWFC
	Domain	198 – 243	46	TSP type-1
	Domain	256 – 330	75	CTCK
	Region	247 – 349	103	Heparin-binding By similarity
Amino acid modifications
	Disulfide bond	256 ↔ 293		By similarity
	Disulfide bond	273 ↔ 307		By similarity
	Disulfide bond	284 ↔ 323		By similarity
	Disulfide bond	287 ↔ 325		By similarity
	Disulfide bond	292 ↔ 329		By similarity
Experimental info
	Sequence conflict	28	1	D → DC in AAB66596. Ref.1
	Sequence conflict	58	1	Missing in AAB66596. Ref.1
	Sequence conflict	88	1	A → T in AAB66596. Ref.1
	Sequence conflict	103 – 104	2	CV → YI in AAB66596. Ref.1
	Sequence conflict	166 – 169	4	CDEP → SRDE in AAB66596. Ref.1
	Sequence conflict	184	1	P → L in AAB66596. Ref.1
	Sequence conflict	200	1	L → Q in AAB66596. Ref.1
	Sequence conflict	209	1	C → Y in AAB66596. Ref.1
	Sequence conflict	269	1	E → Q in AAB66596. Ref.1
	Sequence conflict	284	1	C → F in AAB66596. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O18739 [UniParc]. Last modified February 11, 2002. Version 2. Checksum: 5FFC8EE83EFB4F99	FASTA	349	37,924
	10 20 30 40 50 60 MSATGLGPVR CAFVLLLALC SRPASSQDCS APCQCPAGPA PRCPAGVSLV LDGCGCCRVC 70 80 90 100 110 120 AKQLSELCTE RDPCDPHKGL FCDFGSPANR KIGVCTAKDG APCVFGGTVY QSGESFQSSC 130 140 150 160 170 180 KYQCTCLDGS VGCVPLCSVD VRLPSPDCPF PRRVKLPGKC CEEWVCDEPK EHTVVGPALA 190 200 210 220 230 240 AYRPEDTFGP DPTMIRANCL VQTTEWSACS KTCGMGISTR VTNDNAFCRL EKQSRLCMVR 250 260 270 280 290 300 PCEADLEENI KKGKKCIRTP KISKPIKFEL SGCTSMKTYR AKFCGVCTDG RCCTPHRTTT 310 320 330 340 LPVEFKCPDG EVMKKSMMFI KTCACHYNCP GDNDIFESLY YRKMYGDMA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Bos taurus connective tissue growth factor." Liliensiek B., Lin Z., Fotsis T., Schimanski M., Bierhaus A., Kanitz M., Kauffmann G., Schweigerer L., Ziegler R., Nawroth P.P. Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Aorta.
[2]	"Bovine connective tissue growth factor, organization of the chromosomal gene and demonstration of promoter activity." Mathahs M., Schwitters C., Hove M., Rupp S., Erondu N.E. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Liver.
[3]	NIH - Mammalian Gene Collection (MGC) project Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Uterus.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF000137 mRNA. Translation: AAB66596.1. AF309555 Genomic DNA. Translation: AAG30290.1. BC113279 mRNA. Translation: AAI13280.1.
IPI	IPI00698668.
RefSeq	NP_776455.1. NM_174030.2.
UniGene	Bt.5240.
3D structure databases
ProteinModelPortal	O18739.
ModBase	Search...
Proteomic databases
PRIDE	O18739.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSBTAT00000008357; ENSBTAP00000008357; ENSBTAG00000006367.
GeneID	281103.
KEGG	bta:281103.
Organism-specific databases
CTD	1490.
Phylogenomic databases
eggNOG	NOG72934.
GeneTree	ENSGT00550000074368.
HOGENOM	HOG000231462.
HOVERGEN	HBG000635.
InParanoid	O18739.
KO	K06827.
OMA	KTYRAKF.
OrthoDB	EOG44XJGZ.
Gene expression databases
ArrayExpress	O18739.
Family and domain databases
InterPro	IPR006208. Cys_knot. IPR006207. Cys_knot_C. IPR000867. IGFBP-like. IPR012395. IGFBP_CNN. IPR017891. Insulin_GF-bd_Cys-rich_CS. IPR000884. Thrombospondin_1_rpt. IPR001007. VWF_C. [Graphical view]
Pfam	PF00007. Cys_knot. 1 hit. PF00219. IGFBP. 1 hit. PF00090. TSP_1. 1 hit. PF00093. VWC. 1 hit. [Graphical view]
PIRSF	PIRSF036495. IGFBP_rP_CNN. 1 hit.
SMART	SM00041. CT. 1 hit. SM00121. IB. 1 hit. SM00209. TSP1. 1 hit. SM00214. VWC. 1 hit. [Graphical view]
SUPFAM	SSF82895. TSP1. 1 hit.
PROSITE	PS01185. CTCK_1. 1 hit. PS01225. CTCK_2. 1 hit. PS00222. IGFBP_N_1. 1 hit. PS51323. IGFBP_N_2. 1 hit. PS50092. TSP1. 1 hit. PS01208. VWFC_1. 1 hit. PS50184. VWFC_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20805175.

Entry information

Entry name

CTGF_BOVIN

Accession

Primary (citable) accession number: O18739
Secondary accession number(s): Q2HJ71, Q9GL71

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	February 11, 2002
Last modified:	April 3, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program