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Protein

Protein phosphatase 1 regulatory subunit 14A

Gene

CPI17

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibitor of PPP1CA. Has over 1000-fold higher inhibitory activity when phosphorylated, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction.4 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionProtein phosphatase inhibitor

Enzyme and pathway databases

ReactomeiR-SSC-5625740. RHO GTPases activate PKNs.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein phosphatase 1 regulatory subunit 14A
Alternative name(s):
17 kDa PKC-potentiated inhibitory protein of PP1
Protein kinase C-potentiated inhibitor protein of 17 kDa
Short name:
CPI-17
Gene namesi
Name:CPI17
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi38T → A: Abolishes phosphorylation (in vitro) and strongly reduces inhibitory activity. 3 Publications1
Mutagenesisi38T → E: Reduces inhibitory activity at least 1000-fold. 3 Publications1
Mutagenesisi41Y → A: Promotes dephosphorylation of T-38. Abolishes phosphorylation-dependent increase of inhibitory activity. 1 Publication1
Mutagenesisi42D → A: Strongly reduces inhibitory activity. 1 Publication1
Mutagenesisi43R → A: Strongly reduces inhibitory activity. 1 Publication1
Mutagenesisi44R → A: Reduces inhibitory activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000714881 – 147Protein phosphatase 1 regulatory subunit 14AAdd BLAST147

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei26PhosphoserineBy similarity1
Modified residuei38Phosphothreonine3 Publications1
Modified residuei128PhosphoserineBy similarity1
Modified residuei134PhosphoserineBy similarity1
Modified residuei136PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation of Thr-38 induces a conformation change.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO18734.
PeptideAtlasiO18734.
PRIDEiO18734.

PTM databases

iPTMnetiO18734.

Expressioni

Tissue specificityi

Detected in aorta smooth muscle and bladder.1 Publication

Gene expression databases

BgeeiENSSSCG00000023212.
GenevisibleiO18734. SS.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PPP1R12AO149742EBI-15674919,EBI-351726From Homo sapiens.

Protein-protein interaction databases

DIPiDIP-48426N.
IntActiO18734. 1 interactor.
STRINGi9823.ENSSSCP00000021211.

Structurei

Secondary structure

1147
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi24 – 26Combined sources3
Beta strandi34 – 37Combined sources4
Beta strandi45 – 47Combined sources3
Helixi48 – 62Combined sources15
Helixi65 – 67Combined sources3
Turni68 – 70Combined sources3
Helixi78 – 81Combined sources4
Turni84 – 87Combined sources4
Helixi88 – 98Combined sources11
Beta strandi99 – 102Combined sources4
Helixi105 – 114Combined sources10
Turni115 – 118Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J2MNMR-A22-120[»]
1J2NNMR-A22-120[»]
1K5ONMR-A35-120[»]
2RLTNMR-A22-120[»]
ProteinModelPortaliO18734.
SMRiO18734.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO18734.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 120InhibitoryAdd BLAST86

Sequence similaritiesi

Belongs to the PP1 inhibitor family.Curated

Phylogenomic databases

eggNOGiENOG410IZE6. Eukaryota.
ENOG4111Q1J. LUCA.
HOVERGENiHBG054419.
InParanoidiO18734.
KOiK12328.
OMAiLHKQPGF.
OrthoDBiEOG091G122D.
TreeFamiTF105546.

Family and domain databases

Gene3Di1.10.150.220. 1 hit.
InterProiView protein in InterPro
IPR008025. CPI-17.
IPR036658. CPI-17_sf.
PANTHERiPTHR16188. PTHR16188. 1 hit.
PfamiView protein in Pfam
PF05361. PP1_inhibitor. 1 hit.
SUPFAMiSSF81790. SSF81790. 1 hit.

Sequencei

Sequence statusi: Complete.

O18734-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAQRLGKRV LSKLQSPSRA RGPGGSPGGL QKRHARVTVK YDRRELQRRL
60 70 80 90 100
DVEKWIDGRL EELYRGREAD MPDEVNIDEL LELESEEERS RKIQGLLKSC
110 120 130 140
TNPTENFVQE LLVKLRGLHK QPGLRQPSPS GDGSLSPRQD RARTAPP
Length:147
Mass (Da):16,672
Last modified:January 1, 1998 - v1
Checksum:i315DAE461CF78BBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB008376 mRNA. Translation: BAA22995.1.
RefSeqiNP_999502.1. NM_214337.1.
UniGeneiSsc.13002.

Genome annotation databases

GeneIDi397610.
KEGGissc:397610.

Similar proteinsi

Entry informationi

Entry nameiPP14A_PIG
AccessioniPrimary (citable) accession number: O18734
Secondary accession number(s): Q9TQZ8
, Q9TQZ9, Q9TR00, Q9TR01, Q9TR02
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 9, 2004
Last sequence update: January 1, 1998
Last modified: November 22, 2017
This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families