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Protein

Glutathione S-transferase

Gene
N/A
Organism
Blattella germanica (German cockroach) (Blatta germanica)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

RX + glutathione = HX + R-S-glutathione.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei9 – 91GlutathioneBy similarity
Binding sitei40 – 401GlutathioneBy similarity
Binding sitei44 – 441GlutathioneBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Names & Taxonomyi

Protein namesi
Recommended name:
Glutathione S-transferase (EC:2.5.1.18)
Alternative name(s):
GST class-sigma
Major allergen Bla g 5
Allergen: Bla g 5
OrganismiBlattella germanica (German cockroach) (Blatta germanica)
Taxonomic identifieri6973 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraOrthopteroideaDictyopteraBlattodeaBlaberoideaEctobiidaeBlattellinaeBlattella

Pathology & Biotechi

Allergenic propertiesi

Causes an allergic reaction in human. Causes positive immediate skin tests in cockroach-allergic patients using as little as 3 pg of recombinant protein. Associated with asthma.

Keywords - Diseasei

Allergen

Protein family/group databases

Allergomei144. Bla g 5.
3142. Bla g 5.0101.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 204203Glutathione S-transferasePRO_0000185916Add
BLAST

Structurei

Secondary structure

1
204
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 95Combined sources
Beta strandi11 – 133Combined sources
Turni14 – 163Combined sources
Helixi17 – 259Combined sources
Beta strandi31 – 344Combined sources
Helixi37 – 393Combined sources
Helixi40 – 434Combined sources
Helixi44 – 463Combined sources
Beta strandi54 – 574Combined sources
Beta strandi60 – 634Combined sources
Helixi65 – 7511Combined sources
Helixi83 – 10725Combined sources
Helixi112 – 12413Combined sources
Helixi126 – 14015Combined sources
Beta strandi143 – 1464Combined sources
Helixi152 – 16817Combined sources
Turni172 – 1754Combined sources
Helixi177 – 18812Combined sources
Helixi190 – 1989Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4Q5RX-ray2.25A/B/C/D/E/F1-204[»]
ProteinModelPortaliO18598.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 8078GST N-terminalAdd
BLAST
Domaini82 – 204123GST C-terminalAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni64 – 652Glutathione bindingBy similarity

Sequence similaritiesi

Belongs to the GST superfamily. Sigma family.Curated
Contains 1 GST C-terminal domain.Curated
Contains 1 GST N-terminal domain.Curated

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O18598-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPSYKLTYC PVKALGEPIR FLLSYGEKDF EDYRFQEGDW PNLKPSMPFG
60 70 80 90 100
KTPVLEIDGK QTHQSVAISR YLGKQFGLSG KDDWENLEID MIVDTISDFR
110 120 130 140 150
AAIANYHYDA DENSKQKKWD PLKKETIPYY TKKFDEVVKA NGGYLAAGKL
160 170 180 190 200
TWADFYFVAI LDYLNHMAKE DLVANQPNLK ALREKVLGLP AIKAWVAKRP

PTDL
Length:204
Mass (Da):23,334
Last modified:January 23, 2007 - v3
Checksum:i4CBE9339C913551C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 101C → F AA sequence (PubMed:9252418).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92412 mRNA. Translation: AAB72147.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U92412 mRNA. Translation: AAB72147.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4Q5RX-ray2.25A/B/C/D/E/F1-204[»]
ProteinModelPortaliO18598.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei144. Bla g 5.
3142. Bla g 5.0101.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di1.20.1050.10. 1 hit.
3.40.30.10. 1 hit.
InterProiIPR010987. Glutathione-S-Trfase_C-like.
IPR004045. Glutathione_S-Trfase_N.
IPR004046. GST_C.
IPR012336. Thioredoxin-like_fold.
[Graphical view]
PfamiPF00043. GST_C. 1 hit.
PF02798. GST_N. 1 hit.
[Graphical view]
SUPFAMiSSF47616. SSF47616. 1 hit.
SSF52833. SSF52833. 1 hit.
PROSITEiPS50405. GST_CTER. 1 hit.
PS50404. GST_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Induction of IgE antibody responses by glutathione S-transferase from the German cockroach (Blattella germanica)."
    Arruda L.K., Vailes L.D., Platts-Mills T.A.E., Hayden M.L., Chapman M.D.
    J. Biol. Chem. 272:20907-20912(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-204, PROTEIN SEQUENCE OF 2-39.

Entry informationi

Entry nameiGST1_BLAGE
AccessioniPrimary (citable) accession number: O18598
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 76 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. Allergens
    Nomenclature of allergens and list of entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.