O18598 (GST1_BLAGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Glutathione S-transferase EC=2.5.1.18 Alternative name(s): GST class-sigma Major allergen Bla g 5 Allergen=Bla g 5
Organism	Blattella germanica (German cockroach) (Blatta germanica)
Taxonomic identifier	6973 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Orthopteroidea › Dictyoptera › Blattodea › Blaberoidea › Ectobiidae › Blattellinae › Blattella

Protein attributes

Sequence length	204 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	RX + glutathione = HX + R-S-glutathione.
Allergenic properties	Causes an allergic reaction in human. Causes positive immediate skin tests in cockroach-allergic patients using as little as 3 pg of recombinant protein. Associated with asthma.
Sequence similarities	Belongs to the GST superfamily. Sigma family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Disease	Allergen
Molecular function	Transferase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Molecular_function	glutathione transferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 204	203	Glutathione S-transferase		PRO_0000185916
Regions
	Domain	3 – 80	78	GST N-terminal
	Domain	82 – 204	123	GST C-terminal
	Region	64 – 65	2	Glutathione binding By similarity
Sites
	Binding site	9	1	Glutathione By similarity
	Binding site	40	1	Glutathione By similarity
	Binding site	44	1	Glutathione By similarity
Experimental info
	Sequence conflict	10	1	C → F AA sequence Ref.1

Sequences

	Sequence		Length	Mass (Da)
	O18598 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 4CBE9339C913551C	FASTA	204	23,334
	10 20 30 40 50 60 MAPSYKLTYC PVKALGEPIR FLLSYGEKDF EDYRFQEGDW PNLKPSMPFG KTPVLEIDGK 70 80 90 100 110 120 QTHQSVAISR YLGKQFGLSG KDDWENLEID MIVDTISDFR AAIANYHYDA DENSKQKKWD 130 140 150 160 170 180 PLKKETIPYY TKKFDEVVKA NGGYLAAGKL TWADFYFVAI LDYLNHMAKE DLVANQPNLK 190 200 ALREKVLGLP AIKAWVAKRP PTDL

References

[1]

"Induction of IgE antibody responses by glutathione S-transferase from the German cockroach (Blattella germanica)." Arruda L.K., Vailes L.D., Platts-Mills T.A.E., Hayden M.L., Chapman M.D. J. Biol. Chem. 272:20907-20912(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 5-204, PROTEIN SEQUENCE OF 2-39.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U92412 mRNA. Translation: AAB72147.1.
3D structure databases
ProteinModelPortal	O18598.
ModBase	Search...
Protein family/group databases
Allergome	144. Bla g 5. 3142. Bla g 5.0101.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR004046. GST_C. IPR012336. Thioredoxin-like_fold. [Graphical view]
Pfam	PF00043. GST_C. 1 hit. PF02798. GST_N. 1 hit. [Graphical view]
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GST1_BLAGE

Accession

Primary (citable) accession number: O18598

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 70 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)