Alpha-amylase-related protein precursor - Drosophila pseudoobscura pseudoobscura (Fruit fly)

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O18552 (AMYR_DROPS) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 93. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Alpha-amylase-related protein
EC=3.2.1.1

Gene names

Name:	Amyrel
ORF Names:	GA20907

Organism

Drosophila pseudoobscura pseudoobscura (Fruit fly)

Taxonomic identifier

46245 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	494 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.
Cofactor	Binds 1 calcium ion per subunit By similarity. Binds 1 chloride ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Secreted Probable.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.
Sequence caution	The sequence EAL26626.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Chloride Metal-binding
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond Pyrrolidone carboxylic acid
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	alpha-amylase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 20

By similarity

Chain

21 – 494

474

Alpha-amylase-related protein

PRO_0000001385

Sites

Active site

208

Nucleophile By similarity

Active site

245

Proton donor By similarity

Metal binding

118

Calcium By similarity

Metal binding

169

Calcium; via carbonyl oxygen By similarity

Metal binding

178

Calcium By similarity

Metal binding

212

Calcium; via carbonyl oxygen By similarity

Binding site

206

Chloride By similarity

Binding site

308

Chloride By similarity

Binding site

343

Chloride By similarity

Site

310

Transition state stabilizer By similarity

Amino acid modifications

Modified residue

Pyrrolidone carboxylic acid By similarity

Disulfide bond

48 ↔ 104

By similarity

Disulfide bond

157 ↔ 171

By similarity

Disulfide bond

376 ↔ 382

By similarity

Disulfide bond

418 ↔ 441

Potential

Disulfide bond

448 ↔ 460

By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

O18552 [UniParc].

Last modified May 16, 2006. Version 2.
Checksum: 95C0443078F4FC3B
Show »

FASTA

494

55,590

        10         20         30         40         50         60 
MFKFTFALAL CVLAAGLVLA QHNPHWWGNR NTIVHLFEWK WEDIAEECEN FLGPRGFAGV 

        70         80         90        100        110        120 
QVSPANENIV SPGRPWWERY QPISYKLITR SGNEEQFADM VRRCNEVGVR IYVDVLLNHM 

       130        140        150        160        170        180 
SADFVGQAVG TAGTEADPAV KSFPGVPYSA EDFHPSCEIY DWNDRYQIQH CELVGLKDLD 

       190        200        210        220        230        240 
QSRDWVRTKL IEFLDHLIEL GVAGFRVDAA KHMASEDLEF IYGSLSDLKI EHGFPHSSRP 

       250        260        270        280        290        300 
FIFQEVIDHG GQEVTREEYN SLGAVTEFRF SQEIGNAFRG NNALKWLQSW GTDWGFLSSG 

       310        320        330        340        350        360 
QALTFVDNHD NQRDGGAVLT YKSPRQYKMA TAFHLAYPYG ISRVMSSFAF DDHDSAPPQD 

       370        380        390        400        410        420 
AQEELISPEF DADGGCANGW ICEHRWRQIY NMVGFKNAVR DTEISNWWDN GDSQIAFCRG 

       430        440        450        460        470        480 
SKGFIAFNNN MYNLAEVLQT CLPKGVYCDV ISGDLINGSC SGKSVYVGDD GLGFVSIDSD 

       490 
DFDGVLAIHV DARI

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Amyrel, a paralogous gene of the amylase gene family in Drosophila melanogaster and the Sophophora subgenus." Da Lage J.-L., Renard E., Chartois F., Lemeunier F., Cariou M.-L. Proc. Natl. Acad. Sci. U.S.A. 95:6848-6853(1998) [PubMed: 9618501] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Phoenix.
[2]	Da Lage J.-L. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution." Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L. Gibbs R.A. Genome Res. 15:1-18(2005) [PubMed: 15632085] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MV2-25 / Tucson 14011-0121.94.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U82556 Genomic DNA. Translation: AAC48346.3. CM000071 Genomic DNA. Translation: EAL26626.1. Sequence problems.
RefSeq	XP_001362046.1. XM_001362009.2.
3D structure databases
ProteinModelPortal	O18552.
SMR	O18552. Positions 21-494.
ModBase	Search...
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4805687.
GenomeReviews	Gene locus Amyrel in contig CM000071_GR.
KEGG	dpo:Dpse_GA20907.
Organism-specific databases
FlyBase	FBgn0243574. Dpse\Amyrel.
Phylogenomic databases
InParanoid	O18552.
OMA	GNRNTIV.
OrthoDB	EOG4XD26M.
PhylomeDB	O18552.
Family and domain databases
InterPro	IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR006046. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR006589. Glyco_hydro_13_sub_cat_dom. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KO	K01176.
PANTHER	PTHR10357. Alpha_amylase. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. [Graphical view]
PRINTS	PR00110. ALPHAAMYLASE.
SMART	SM00642. Aamy. 1 hit. SM00632. Aamy_C. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AMYR_DROPS

Accession

Primary (citable) accession number: O18552
Secondary accession number(s): Q28WP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	May 16, 2006
Last modified:	December 14, 2011
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

Drosophila

Drosophila: entries, gene names and cross-references to FlyBase

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents