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Protein

Alpha-amylase-related protein

Gene

Amyrel

Organism
Drosophila pseudoobscura pseudoobscura (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
  • chlorideBy similarityNote: Binds 1 Cl- ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi118CalciumBy similarity1
Metal bindingi169Calcium; via carbonyl oxygenBy similarity1
Metal bindingi178CalciumBy similarity1
Binding sitei206ChlorideBy similarity1
Active sitei208NucleophileBy similarity1
Metal bindingi212Calcium; via carbonyl oxygenBy similarity1
Active sitei245Proton donorBy similarity1
Binding sitei308ChlorideBy similarity1
Sitei310Transition state stabilizerBy similarity1
Binding sitei343ChlorideBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Chloride, Metal-binding

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase-related protein (EC:3.2.1.1By similarity)
Gene namesi
Name:Amyrel
ORF Names:GA20907
OrganismiDrosophila pseudoobscura pseudoobscura (Fruit fly)
Taxonomic identifieri46245 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000001819 Componentsi: Chromosome 3, Partially assembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0243574. Dpse\Amyrel.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20By similarityAdd BLAST20
ChainiPRO_000000138521 – 494Alpha-amylase-related proteinAdd BLAST474

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei21Pyrrolidone carboxylic acidBy similarity1
Disulfide bondi48 ↔ 104By similarity
Disulfide bondi157 ↔ 171By similarity
Disulfide bondi376 ↔ 382By similarity
Disulfide bondi418 ↔ 441Sequence analysis
Disulfide bondi448 ↔ 460By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

Monomer.By similarity

Structurei

3D structure databases

ProteinModelPortaliO18552.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

InParanoidiO18552.
KOiK01176.
OMAiDTEISNW.
PhylomeDBiO18552.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O18552-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKFTFALAL CVLAAGLVLA QHNPHWWGNR NTIVHLFEWK WEDIAEECEN
60 70 80 90 100
FLGPRGFAGV QVSPANENIV SPGRPWWERY QPISYKLITR SGNEEQFADM
110 120 130 140 150
VRRCNEVGVR IYVDVLLNHM SADFVGQAVG TAGTEADPAV KSFPGVPYSA
160 170 180 190 200
EDFHPSCEIY DWNDRYQIQH CELVGLKDLD QSRDWVRTKL IEFLDHLIEL
210 220 230 240 250
GVAGFRVDAA KHMASEDLEF IYGSLSDLKI EHGFPHSSRP FIFQEVIDHG
260 270 280 290 300
GQEVTREEYN SLGAVTEFRF SQEIGNAFRG NNALKWLQSW GTDWGFLSSG
310 320 330 340 350
QALTFVDNHD NQRDGGAVLT YKSPRQYKMA TAFHLAYPYG ISRVMSSFAF
360 370 380 390 400
DDHDSAPPQD AQEELISPEF DADGGCANGW ICEHRWRQIY NMVGFKNAVR
410 420 430 440 450
DTEISNWWDN GDSQIAFCRG SKGFIAFNNN MYNLAEVLQT CLPKGVYCDV
460 470 480 490
ISGDLINGSC SGKSVYVGDD GLGFVSIDSD DFDGVLAIHV DARI
Length:494
Mass (Da):55,590
Last modified:May 16, 2006 - v2
Checksum:i95C0443078F4FC3B
GO

Sequence cautioni

The sequence EAL26626 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82556 Genomic DNA. Translation: AAC48346.3.
CM000071 Genomic DNA. Translation: EAL26626.1. Sequence problems.
RefSeqiXP_001362046.2. XM_001362009.3.

Genome annotation databases

EnsemblMetazoaiFBtr0280356; FBpp0278794; FBgn0243574.
GeneIDi4805687.
KEGGidpo:Dpse_GA20907.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U82556 Genomic DNA. Translation: AAC48346.3.
CM000071 Genomic DNA. Translation: EAL26626.1. Sequence problems.
RefSeqiXP_001362046.2. XM_001362009.3.

3D structure databases

ProteinModelPortaliO18552.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0280356; FBpp0278794; FBgn0243574.
GeneIDi4805687.
KEGGidpo:Dpse_GA20907.

Organism-specific databases

FlyBaseiFBgn0243574. Dpse\Amyrel.

Phylogenomic databases

InParanoidiO18552.
KOiK01176.
OMAiDTEISNW.
PhylomeDBiO18552.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMYR_DROPS
AccessioniPrimary (citable) accession number: O18552
Secondary accession number(s): Q28WP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: May 16, 2006
Last modified: October 5, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.