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Protein

26S proteasome regulatory subunit 8

Gene

Rpt6

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi189 – 196ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.4.25.1. 1994.
ReactomeiR-DME-1169091. Activation of NF-kappaB in B cells.
R-DME-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-DME-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-DME-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-DME-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-DME-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-DME-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-DME-195253. Degradation of beta-catenin by the destruction complex.
R-DME-202424. Downstream TCR signaling.
R-DME-2871837. FCERI mediated NF-kB activation.
R-DME-350562. Regulation of ornithine decarboxylase (ODC).
R-DME-446652. Interleukin-1 family signaling.
R-DME-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-DME-4608870. Asymmetric localization of PCP proteins.
R-DME-4641257. Degradation of AXIN.
R-DME-4641258. Degradation of DVL.
R-DME-5358346. Hedgehog ligand biogenesis.
R-DME-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-DME-5607764. CLEC7A (Dectin-1) signaling.
R-DME-5610780. Degradation of GLI1 by the proteasome.
R-DME-5610785. GLI3 is processed to GLI3R by the proteasome.
R-DME-5632684. Hedgehog 'on' state.
R-DME-5658442. Regulation of RAS by GAPs.
R-DME-5676590. NIK-->noncanonical NF-kB signaling.
R-DME-5689603. UCH proteinases.
R-DME-5689880. Ub-specific processing proteases.
R-DME-68949. Orc1 removal from chromatin.
R-DME-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-DME-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-DME-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-DME-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-DME-8941858. Regulation of RUNX3 expression and activity.
R-DME-8948751. Regulation of PTEN stability and activity.
R-DME-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit 8
Gene namesi
Name:Rpt6
Synonyms:DUG, Pros45, Ug
ORF Names:CG1489
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0020369. Rpt6.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000847271 – 40526S proteasome regulatory subunit 8Add BLAST405

Proteomic databases

PaxDbiO18413.
PRIDEiO18413.

Expressioni

Gene expression databases

BgeeiFBgn0020369.
GenevisibleiO18413. DM.

Interactioni

Subunit structurei

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. Interacts with Rpn6.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Atg6Q9VCE13EBI-174806,EBI-134130

GO - Molecular functioni

Protein-protein interaction databases

BioGridi59383. 72 interactors.
DIPiDIP-20087N.
IntActiO18413. 24 interactors.
MINTiMINT-759582.
STRINGi7227.FBpp0076890.

Structurei

3D structure databases

ProteinModelPortaliO18413.
SMRiO18413.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

eggNOGiKOG0728. Eukaryota.
COG1222. LUCA.
GeneTreeiENSGT00550000074947.
InParanoidiO18413.
KOiK03066.
OMAiHGNNQEA.
OrthoDBiEOG091G096W.
PhylomeDBiO18413.

Family and domain databases

InterProiView protein in InterPro
IPR005937. 26S_Psome_P45-like.
IPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR003960. ATPase_AAA_CS.
IPR027417. P-loop_NTPase.
IPR035261. PSMC5.
PANTHERiPTHR23073:SF47. PTHR23073:SF47. 1 hit.
PfamiView protein in Pfam
PF00004. AAA. 1 hit.
SMARTiView protein in SMART
SM00382. AAA. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01242. 26Sp45. 1 hit.
PROSITEiView protein in PROSITE
PS00674. AAA. 1 hit.

Sequencei

Sequence statusi: Complete.

O18413-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVTNRMEIE SAYHKGEGFR SYYIQKIEEL QLVVAEKHQN LRRLQAQRNE
60 70 80 90 100
LNAKVRMLRE ELQLLQEQGS YVGEVVKPMD KKKVLVKVHP EGKFVVDLDK
110 120 130 140 150
NIDINDVTPN CRVALRNESY TLHKILPNKV DPLVSLMMVE KVPDSTYEMV
160 170 180 190 200
GGLDKQIKEI KEVIELPVKH PELFDALGIA QPKGVLLYGP PGTGKTLLAR
210 220 230 240 250
AVAHHTECTF IRVSGSELVQ KFIGEGSRMV RELFVMAREH APSIIFMDEI
260 270 280 290 300
DSIGSSRIES GSGGDSEVQR TMLELLNQLD GFEATKNIKV IMATNRIDIL
310 320 330 340 350
DPALLRPGRI DRKIEFPPPN EEARLDILKI HSRKMNLTRG INLRKIAELM
360 370 380 390 400
PGASGAEVKG VCTEAGMYAL RERRVHVTQE DFEMAVAKVM QKDSEKNMSI

KKLWK
Length:405
Mass (Da):45,857
Last modified:June 1, 2001 - v2
Checksum:iC098EEE56B348211
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti94F → S in AAC48284 (PubMed:9469929).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97538 mRNA. Translation: AAC48284.1.
AF043734 mRNA. Translation: AAC63219.1.
AE014298 Genomic DNA. Translation: AAF50835.1.
AY051732 mRNA. Translation: AAK93156.1.
RefSeqiNP_608447.1. NM_134603.2.
UniGeneiDm.2162.

Genome annotation databases

EnsemblMetazoaiFBtr0077189; FBpp0076890; FBgn0020369.
GeneIDi33105.
KEGGidme:Dmel_CG1489.

Similar proteinsi

Entry informationi

Entry nameiPRS8_DROME
AccessioniPrimary (citable) accession number: O18413
Secondary accession number(s): O45023
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: June 1, 2001
Last modified: September 27, 2017
This is version 154 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families