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Protein

26S proteasome regulatory subunit 8

Gene

Rpt6

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi189 – 196ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.4.25.1 1994
ReactomeiR-DME-1169091 Activation of NF-kappaB in B cells
R-DME-1234176 Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha
R-DME-1236978 Cross-presentation of soluble exogenous antigens (endosomes)
R-DME-174084 Autodegradation of Cdh1 by Cdh1:APC/C
R-DME-174113 SCF-beta-TrCP mediated degradation of Emi1
R-DME-174178 APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1
R-DME-174184 Cdc20:Phospho-APC/C mediated degradation of Cyclin A
R-DME-187577 SCF(Skp2)-mediated degradation of p27/p21
R-DME-195253 Degradation of beta-catenin by the destruction complex
R-DME-202424 Downstream TCR signaling
R-DME-209360 Ubiquitination and proteolysis of phosphorylated CI
R-DME-209406 Degradation of NF-kappa-B inhibitor, CACT
R-DME-209461 Ubiquitination and degradation of phosphorylated ARM
R-DME-216167 Nuclear CI is degraded
R-DME-2871837 FCERI mediated NF-kB activation
R-DME-432395 Degradation of TIM
R-DME-432524 Degradation of PER
R-DME-450408 AUF1 (hnRNP D0) binds and destabilizes mRNA
R-DME-4608870 Asymmetric localization of PCP proteins
R-DME-4641257 Degradation of AXIN
R-DME-4641258 Degradation of DVL
R-DME-5358346 Hedgehog ligand biogenesis
R-DME-538848 Degradation of CLK
R-DME-538864 Degradation of CRY
R-DME-5607761 Dectin-1 mediated noncanonical NF-kB signaling
R-DME-5607764 CLEC7A (Dectin-1) signaling
R-DME-5610780 Degradation of GLI1 by the proteasome
R-DME-5610785 GLI3 is processed to GLI3R by the proteasome
R-DME-5632684 Hedgehog 'on' state
R-DME-5658442 Regulation of RAS by GAPs
R-DME-5676590 NIK-->noncanonical NF-kB signaling
R-DME-5689603 UCH proteinases
R-DME-5689880 Ub-specific processing proteases
R-DME-68949 Orc1 removal from chromatin
R-DME-69017 CDK-mediated phosphorylation and removal of Cdc6
R-DME-69229 Ubiquitin-dependent degradation of Cyclin D1
R-DME-69601 Ubiquitin Mediated Degradation of Phosphorylated Cdc25A
R-DME-8854050 FBXL7 down-regulates AURKA during mitotic entry and in early mitosis
R-DME-8939902 Regulation of RUNX2 expression and activity
R-DME-8941858 Regulation of RUNX3 expression and activity
R-DME-8948751 Regulation of PTEN stability and activity
R-DME-9020702 Interleukin-1 signaling
R-DME-983168 Antigen processing: Ubiquitination & Proteasome degradation

Names & Taxonomyi

Protein namesi
Recommended name:
26S proteasome regulatory subunit 8
Gene namesi
Name:Rpt6
Synonyms:DUG, Pros45, Ug
ORF Names:CG1489
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome X

Organism-specific databases

FlyBaseiFBgn0020369 Rpt6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000847271 – 40526S proteasome regulatory subunit 8Add BLAST405

Proteomic databases

PaxDbiO18413
PRIDEiO18413

Expressioni

Gene expression databases

BgeeiFBgn0020369
GenevisibleiO18413 DM

Interactioni

Subunit structurei

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. Interacts with Rpn6 (PubMed:22187461). Interacts with imd and Usp2 isoform A (PubMed:25027767).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Atg6Q9VCE13EBI-174806,EBI-134130

GO - Molecular functioni

Protein-protein interaction databases

BioGridi59383, 72 interactors
DIPiDIP-20087N
IntActiO18413, 24 interactors
STRINGi7227.FBpp0076890

Structurei

3D structure databases

ProteinModelPortaliO18413
SMRiO18413
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AAA ATPase family.Curated

Phylogenomic databases

eggNOGiKOG0728 Eukaryota
COG1222 LUCA
GeneTreeiENSGT00550000074947
InParanoidiO18413
KOiK03066
OMAiHGNNQEA
OrthoDBiEOG091G096W
PhylomeDBiO18413

Family and domain databases

InterProiView protein in InterPro
IPR005937 26S_Psome_P45-like
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR003960 ATPase_AAA_CS
IPR027417 P-loop_NTPase
IPR035261 PSMC5
PANTHERiPTHR23073:SF12 PTHR23073:SF12, 1 hit
PfamiView protein in Pfam
PF00004 AAA, 1 hit
SMARTiView protein in SMART
SM00382 AAA, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01242 26Sp45, 1 hit
PROSITEiView protein in PROSITE
PS00674 AAA, 1 hit

Sequencei

Sequence statusi: Complete.

O18413-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTVTNRMEIE SAYHKGEGFR SYYIQKIEEL QLVVAEKHQN LRRLQAQRNE
60 70 80 90 100
LNAKVRMLRE ELQLLQEQGS YVGEVVKPMD KKKVLVKVHP EGKFVVDLDK
110 120 130 140 150
NIDINDVTPN CRVALRNESY TLHKILPNKV DPLVSLMMVE KVPDSTYEMV
160 170 180 190 200
GGLDKQIKEI KEVIELPVKH PELFDALGIA QPKGVLLYGP PGTGKTLLAR
210 220 230 240 250
AVAHHTECTF IRVSGSELVQ KFIGEGSRMV RELFVMAREH APSIIFMDEI
260 270 280 290 300
DSIGSSRIES GSGGDSEVQR TMLELLNQLD GFEATKNIKV IMATNRIDIL
310 320 330 340 350
DPALLRPGRI DRKIEFPPPN EEARLDILKI HSRKMNLTRG INLRKIAELM
360 370 380 390 400
PGASGAEVKG VCTEAGMYAL RERRVHVTQE DFEMAVAKVM QKDSEKNMSI

KKLWK
Length:405
Mass (Da):45,857
Last modified:June 1, 2001 - v2
Checksum:iC098EEE56B348211
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti94F → S in AAC48284 (PubMed:9469929).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U97538 mRNA Translation: AAC48284.1
AF043734 mRNA Translation: AAC63219.1
AE014298 Genomic DNA Translation: AAF50835.1
AY051732 mRNA Translation: AAK93156.1
RefSeqiNP_608447.1, NM_134603.2
UniGeneiDm.2162

Genome annotation databases

EnsemblMetazoaiFBtr0077189; FBpp0076890; FBgn0020369
GeneIDi33105
KEGGidme:Dmel_CG1489

Similar proteinsi

Entry informationi

Entry nameiPRS8_DROME
AccessioniPrimary (citable) accession number: O18413
Secondary accession number(s): O45023
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: June 1, 2001
Last modified: March 28, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health