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Protein

Alpha-amylase-related protein

Gene

Amyrel

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
  • chlorideBy similarityNote: Binds 1 Cl- ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi117 – 1171CalciumBy similarity
Metal bindingi168 – 1681Calcium; via carbonyl oxygenBy similarity
Metal bindingi177 – 1771CalciumBy similarity
Binding sitei205 – 2051ChlorideBy similarity
Active sitei207 – 2071NucleophileBy similarity
Metal bindingi211 – 2111Calcium; via carbonyl oxygenBy similarity
Active sitei244 – 2441Proton donorBy similarity
Binding sitei307 – 3071ChlorideBy similarity
Sitei309 – 3091Transition state stabilizerBy similarity
Binding sitei342 – 3421ChlorideBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Chloride, Metal-binding

Enzyme and pathway databases

ReactomeiR-DME-189085. Digestion of dietary carbohydrate.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase-related protein (EC:3.2.1.1By similarity)
Gene namesi
Name:Amyrel
ORF Names:CG8221
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0020506. Amyrel.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919By similarityAdd
BLAST
Chaini20 – 493474Alpha-amylase-related proteinPRO_0000001382Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei20 – 201Pyrrolidone carboxylic acidBy similarity
Disulfide bondi47 ↔ 103By similarity
Disulfide bondi156 ↔ 170By similarity
Disulfide bondi375 ↔ 381By similarity
Disulfide bondi417 ↔ 440Sequence analysis
Disulfide bondi447 ↔ 459By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiO18408.

Expressioni

Tissue specificityi

Midgut and fat body.

Developmental stagei

Expressed during second and third larval instars, but not in the adult.

Gene expression databases

BgeeiFBgn0020506.
GenevisibleiO18408. DM.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi62575. 5 interactions.
IntActiO18408. 3 interactions.
STRINGi7227.FBpp0086263.

Structurei

3D structure databases

ProteinModelPortaliO18408.
SMRiO18408. Positions 29-493.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2212. Eukaryota.
COG0366. LUCA.
GeneTreeiENSGT00390000002882.
InParanoidiO18408.
KOiK01176.
OMAiDTEISNW.
OrthoDBiEOG091G07V6.
PhylomeDBiO18408.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O18408-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKFALTLTL CLAGSLSLAQ HNPHWWGNRN TIVHLFEWKW SDIAQECESF
60 70 80 90 100
LGPRGFAGVQ VSPVNENILS AGRPWWERYQ PISYKLTTRS GNEEEFGDMV
110 120 130 140 150
RRCNDVGVRI YVDVLLNHMS GDFDGVAVGT AGTEAEPSKK SFPGVPYTAQ
160 170 180 190 200
DFHPTCEITD WNDRFQVQQC ELVGLKDLDQ SSDWVRSKLI EFLDHLIELG
210 220 230 240 250
VAGFRVDAAK HMASEDLEYI YSSLSNLNID HGFPHNSRPF IFQEVIDHGH
260 270 280 290 300
ETVSRDEYKD LGAVTEFRFS EEIGNAFRGN NALKWLQSWG TDWGFLPSGQ
310 320 330 340 350
ALTFVDNHDN QRDAGAVLNY KSPRQYKMAT AFHLAYPYGI SRVMSSFAFD
360 370 380 390 400
DHDTPPPQDA QERIISPEFD ADGACVNGWI CEHRWRQIYA MVGFKNAVRD
410 420 430 440 450
TEITGWWDNG DNQISFCRGN KGFLAINNNL YDLSQDLNTC LPAGTYCDVI
460 470 480 490
SGSLIDGSCT GKSVTVNENG YGYIHIGSDD FDGVLALHVD AKV
Length:493
Mass (Da):55,451
Last modified:January 11, 2011 - v2
Checksum:i3223298DAF7E634C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21F → S in AAD08845 (Ref. 2) Curated
Sequence conflicti2 – 21F → S in AAD09147 (Ref. 3) Curated
Sequence conflicti7 – 71T → A in AAD08845 (Ref. 2) Curated
Sequence conflicti18 – 181L → V in AAD08845 (Ref. 2) Curated
Sequence conflicti18 – 181L → V in AAD09147 (Ref. 3) Curated
Sequence conflicti69 – 691L → I in AAD08845 (Ref. 2) Curated
Sequence conflicti69 – 691L → I in AAD09147 (Ref. 3) Curated
Sequence conflicti127 – 1271A → V in AAD08845 (Ref. 2) Curated
Sequence conflicti127 – 1271A → V in AAD09147 (Ref. 3) Curated
Sequence conflicti157 – 1571E → V in AAD08845 (Ref. 2) Curated
Sequence conflicti157 – 1571E → V in AAD09147 (Ref. 3) Curated
Sequence conflicti253 – 2531V → D in AAD08845 (Ref. 2) Curated
Sequence conflicti340 – 3401I → T in AAD08845 (Ref. 2) Curated
Sequence conflicti358 – 3581Q → R in AAD08845 (Ref. 2) Curated
Sequence conflicti466 – 4672VN → MS in AAD08845 (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U69607 Genomic DNA. Translation: AAD08845.1.
AF022713 Genomic DNA. Translation: AAD09147.2.
AE013599 Genomic DNA. Translation: AAF57971.1.
AY052055 mRNA. Translation: AAK93479.1.
RefSeqiNP_477262.1. NM_057914.4.
UniGeneiDm.4395.

Genome annotation databases

EnsemblMetazoaiFBtr0087117; FBpp0086263; FBgn0020506.
GeneIDi36863.
KEGGidme:Dmel_CG8221.
UCSCiCG8221-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U69607 Genomic DNA. Translation: AAD08845.1.
AF022713 Genomic DNA. Translation: AAD09147.2.
AE013599 Genomic DNA. Translation: AAF57971.1.
AY052055 mRNA. Translation: AAK93479.1.
RefSeqiNP_477262.1. NM_057914.4.
UniGeneiDm.4395.

3D structure databases

ProteinModelPortaliO18408.
SMRiO18408. Positions 29-493.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi62575. 5 interactions.
IntActiO18408. 3 interactions.
STRINGi7227.FBpp0086263.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Proteomic databases

PaxDbiO18408.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0087117; FBpp0086263; FBgn0020506.
GeneIDi36863.
KEGGidme:Dmel_CG8221.
UCSCiCG8221-RA. d. melanogaster.

Organism-specific databases

CTDi36863.
FlyBaseiFBgn0020506. Amyrel.

Phylogenomic databases

eggNOGiKOG2212. Eukaryota.
COG0366. LUCA.
GeneTreeiENSGT00390000002882.
InParanoidiO18408.
KOiK01176.
OMAiDTEISNW.
OrthoDBiEOG091G07V6.
PhylomeDBiO18408.

Enzyme and pathway databases

ReactomeiR-DME-189085. Digestion of dietary carbohydrate.

Miscellaneous databases

GenomeRNAii36863.
PROiO18408.

Gene expression databases

BgeeiFBgn0020506.
GenevisibleiO18408. DM.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMYR_DROME
AccessioniPrimary (citable) accession number: O18408
Secondary accession number(s): Q9V7S3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 11, 2011
Last modified: September 7, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.