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Protein

Probable serine hydrolase

Gene

kraken

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

May have a role in detoxification and digestion during embryogenesis and larval development.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei138 – 1381By similarity

GO - Molecular functioni

  • carboxylic ester hydrolase activity Source: UniProtKB-KW
  • serine hydrolase activity Source: UniProtKB

GO - Biological processi

  • digestion Source: UniProtKB
  • multicellular organism development Source: UniProtKB-KW
  • response to toxic substance Source: UniProtKB
  • sensory perception of pain Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Hydrolase, Serine esterase

Keywords - Biological processi

Detoxification, Digestion

Protein family/group databases

ESTHERidrome-KRAKEN. AlphaBeta_hydrolase.
MEROPSiS33.A81.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable serine hydrolase (EC:3.1.-.-)
Alternative name(s):
Kraken protein
Gene namesi
Name:kraken
ORF Names:CG3943
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0020545. kraken.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 331331Probable serine hydrolasePRO_0000084323Add
BLAST

Proteomic databases

PaxDbiO18391.
PRIDEiO18391.

Expressioni

Tissue specificityi

Ubiquitously expressed before embryonic stage 11. At stage 11, expression is concentrated in the foregut and posterior midgut. By stage 15, in gastric caeca, pharynx, posterior spiracles and anterior edge of midgut. At the end of embryogenesis, expression is confined to gastric caeca. During third instar larvae, expressed at low levels in gastric caeca, midgut and hindgut and high level in fat body.

Developmental stagei

Probably expressed both maternally and zygotically.

Gene expression databases

BgeeiO18391.
ExpressionAtlasiO18391. differential.
GenevisibleiO18391. DM.

Interactioni

Protein-protein interaction databases

STRINGi7227.FBpp0077681.

Structurei

3D structure databases

ProteinModelPortaliO18391.
SMRiO18391. Positions 57-165.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AB hydrolase superfamily.Curated

Phylogenomic databases

eggNOGiENOG410IPFM. Eukaryota.
ENOG4111NQ7. LUCA.
GeneTreeiENSGT00530000063960.
InParanoidiO18391.
OMAiFIFWDGI.
OrthoDBiEOG7C2R1P.
PhylomeDBiO18391.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O18391-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGQTRVAATT AAQSPAAELS PETNGQTEEP LQLLGEDSWE EFSIAVPWGT
60 70 80 90 100
VEAKWWGSKE RQPIIALHGW QDNCGSFDRL CPLLPADTSI LAIDLPGHGK
110 120 130 140 150
SSHYPMGMQY FIFWDGICLI RRIVRKYNWK NVTLLGHSLG GALTFMYAAS
160 170 180 190 200
FPTEVEKLIN IDIAGPTVRG TQRMAEGTGR ALDKFLDYET LPESKQPCYS
210 220 230 240 250
YDEMIKLVLD AYDGSVDEPS VRVLMNRGMR HNPSKNGYLF ARDLRLKVSL
260 270 280 290 300
LGMFTAEQTL AYARQIRCRV LNIRGIPGMK FETPQVYADV IATLRENAAK
310 320 330
VVYVEVPGTH HLHLVTPDRV APHIIRFLKE A
Length:331
Mass (Da):37,094
Last modified:January 1, 1998 - v1
Checksum:i1FE39BC42AED4E69
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000516 Genomic DNA. Translation: CAA04153.1.
AE014134 Genomic DNA. Translation: AAF51445.1.
AY095092 mRNA. Translation: AAM11420.1.
AY118303 mRNA. Translation: AAM48332.1.
RefSeqiNP_001259847.1. NM_001272918.1.
NP_477265.1. NM_057917.4.
UniGeneiDm.8454.

Genome annotation databases

EnsemblMetazoaiFBtr0078016; FBpp0077681; FBgn0020545.
FBtr0334794; FBpp0306841; FBgn0020545.
GeneIDi33265.
KEGGidme:Dmel_CG3943.
UCSCiCG3943-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000516 Genomic DNA. Translation: CAA04153.1.
AE014134 Genomic DNA. Translation: AAF51445.1.
AY095092 mRNA. Translation: AAM11420.1.
AY118303 mRNA. Translation: AAM48332.1.
RefSeqiNP_001259847.1. NM_001272918.1.
NP_477265.1. NM_057917.4.
UniGeneiDm.8454.

3D structure databases

ProteinModelPortaliO18391.
SMRiO18391. Positions 57-165.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7227.FBpp0077681.

Protein family/group databases

ESTHERidrome-KRAKEN. AlphaBeta_hydrolase.
MEROPSiS33.A81.

Proteomic databases

PaxDbiO18391.
PRIDEiO18391.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0078016; FBpp0077681; FBgn0020545.
FBtr0334794; FBpp0306841; FBgn0020545.
GeneIDi33265.
KEGGidme:Dmel_CG3943.
UCSCiCG3943-RA. d. melanogaster.

Organism-specific databases

CTDi33265.
FlyBaseiFBgn0020545. kraken.

Phylogenomic databases

eggNOGiENOG410IPFM. Eukaryota.
ENOG4111NQ7. LUCA.
GeneTreeiENSGT00530000063960.
InParanoidiO18391.
OMAiFIFWDGI.
OrthoDBiEOG7C2R1P.
PhylomeDBiO18391.

Miscellaneous databases

ChiTaRSikraken. fly.
GenomeRNAii33265.
PROiO18391.

Gene expression databases

BgeeiO18391.
ExpressionAtlasiO18391. differential.
GenevisibleiO18391. DM.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR000073. AB_hydrolase_1.
[Graphical view]
PfamiPF00561. Abhydrolase_1. 1 hit.
[Graphical view]
PRINTSiPR00111. ABHYDROLASE.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00120. LIPASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of kraken, a gene encoding a putative hydrolytic enzyme in Drosophila melanogaster."
    Chan H.Y.E., Harris S.J., O'Kane C.J.
    Gene 222:195-201(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Embryo.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. "A Drosophila full-length cDNA resource."
    Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
    Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Head.

Entry informationi

Entry nameiKRAK_DROME
AccessioniPrimary (citable) accession number: O18391
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: January 1, 1998
Last modified: June 8, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.