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Protein

Selenide, water dikinase

Gene

SelD

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Synthesizes selenophosphate from selenide and ATP. Essential for progression of the cell cycle by controlling the synthesis of selenoproteins. Plays a role in apoptosis and consequently a role in imaginal disk patterning and growth.4 Publications

Catalytic activityi

ATP + selenide + H2O = AMP + selenophosphate + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei51Sequence analysis1
Sitei52Important for catalytic activityBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi290 – 296ATPSequence analysis7

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell proliferation Source: FlyBase
  • glutamine metabolic process Source: FlyBase
  • imaginal disc development Source: FlyBase
  • mitochondrion organization Source: FlyBase
  • negative regulation of reactive oxygen species metabolic process Source: FlyBase
  • positive regulation of cell proliferation Source: FlyBase
  • regulation of glucose metabolic process Source: FlyBase
  • selenocysteine biosynthetic process Source: FlyBase

Keywordsi

Molecular functionDevelopmental protein, Kinase, Transferase
Biological processCell cycle
LigandATP-binding, Nucleotide-binding, Selenium

Enzyme and pathway databases

ReactomeiR-DME-2408557. Selenocysteine synthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Selenide, water dikinase (EC:2.7.9.3)
Alternative name(s):
Protein patufet
Selenium donor protein
Selenophosphate synthase
dSelD
Gene namesi
Name:SelD
Synonyms:PTF1, ptuf
ORF Names:CG8553
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0261270. SelD.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001276521 – 398Selenide, water dikinaseAdd BLAST398

Proteomic databases

PaxDbiO18373.
PRIDEiO18373.

Expressioni

Tissue specificityi

Expressed at low levels throughout the embryo. At later developmental stages, expressed in areas of high cell proliferation. From embryo stage 13, expression is high in central nervous system and midgut, especially in the gastric caeca. Expression also seen in larval imaginal disks and brain.3 Publications

Developmental stagei

Throughout development, from embryo to adult.1 Publication

Gene expression databases

BgeeiFBgn0261270.
GenevisibleiO18373. DM.

Interactioni

Protein-protein interaction databases

BioGridi62337. 12 interactors.
DIPiDIP-21887N.
IntActiO18373. 6 interactors.
MINTiMINT-898227.
STRINGi7227.FBpp0086691.

Structurei

3D structure databases

ProteinModelPortaliO18373.
SMRiO18373.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3939. Eukaryota.
COG0709. LUCA.
GeneTreeiENSGT00390000000950.
InParanoidiO18373.
KOiK01008.
OMAiGCIPGGT.
OrthoDBiEOG091G0B7N.
PhylomeDBiO18373.

Family and domain databases

CDDicd02195. SelD. 1 hit.
Gene3Di3.30.1330.10. 1 hit.
3.90.650.10. 2 hits.
InterProiView protein in InterPro
IPR010918. AIR_synth_C_dom.
IPR016188. PurM-like_N.
IPR023061. SelD_I.
IPR004536. SPS/SelD.
PANTHERiPTHR10256. PTHR10256. 1 hit.
PfamiView protein in Pfam
PF00586. AIRS. 1 hit.
PF02769. AIRS_C. 1 hit.
PIRSFiPIRSF036407. Selenphspht_syn. 1 hit.
SUPFAMiSSF55326. SSF55326. 1 hit.
SSF56042. SSF56042. 1 hit.
TIGRFAMsiTIGR00476. selD. 1 hit.

Sequencei

Sequence statusi: Complete.

O18373-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYAADVLNS AHLELHGGGD AELRRPFDPT AHDLDASFRL TRFADLKGRG
60 70 80 90 100
CKVPQDVLSK LVSALQQDYS AQDQEPQFLN VAIPRIGIGL DCSVIPLRHG
110 120 130 140 150
GLCLVQTTDF FYPIVDDPYM MGKIACANVL SDLYAMGVTD CDNMLMLLAV
160 170 180 190 200
STKMTEKERD VVIPLIMRGF KDSALEAGTT VTGGQSVVNP WCTIGGVAST
210 220 230 240 250
ICQPNEYIVP DNAVVGDVLV LTKPLGTQVA VNAHQWIDQP ERWNRIKLVV
260 270 280 290 300
SEEDVRKAYH RAMNSMARLN RVAARLMHKY NAHGATDITG FGLLGHAQTL
310 320 330 340 350
AAHQKKDVSF VIHNLPVIAK MAAVAKACGN MFQLLQGHSA ETSGGLLICL
360 370 380 390
PREQAAAYCK DIEKQEGYQA WIIGIVEKGN KTARIIDKPR VIEVPAKD
Length:398
Mass (Da):43,446
Last modified:January 1, 1998 - v1
Checksum:iB265DAF4FDACC2D8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti253 – 254ED → KN in AAB88790 (PubMed:9491069).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ000672 mRNA. Translation: CAA04229.1.
U91994 Genomic DNA. Translation: AAB88790.1.
AE013599 Genomic DNA. Translation: AAF58266.1.
AY095058 mRNA. Translation: AAM11386.1.
RefSeqiNP_725374.1. NM_166045.2.
NP_725375.1. NM_166046.2.
UniGeneiDm.2979.

Genome annotation databases

EnsemblMetazoaiFBtr0087564; FBpp0086690; FBgn0261270.
FBtr0087565; FBpp0086691; FBgn0261270.
GeneIDi36587.
KEGGidme:Dmel_CG8553.

Similar proteinsi

Entry informationi

Entry nameiSPS1_DROME
AccessioniPrimary (citable) accession number: O18373
Secondary accession number(s): O18597, Q0E979, Q9V700
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 1, 1998
Last modified: August 30, 2017
This is version 138 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Caution

The conserved active site Cys (or selenocysteine) residue in position 49 is replaced by an Arg. However, as function in selenoprotein synthesis is proven, it is possible Cys-51 is the active site.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families