O18373 (SPS1_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Selenide, water dikinase
EC=2.7.9.3

Alternative name(s):
Protein patufet
Selenium donor protein
Selenophosphate synthase
dSelD

Gene names

Name:	SelD
Synonyms:	PTF1, ptuf
ORF Names:	CG8553

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Synthesizes selenophosphate from selenide and ATP. Essential for progression of the cell cycle by controlling the synthesis of selenoproteins. Plays a role in apoptosis and consequently a role in imaginal disk patterning and growth. Ref.1 Ref.2 Ref.6 Ref.7
Catalytic activity	ATP + selenide + H2O = AMP + selenophosphate + phosphate.
Tissue specificity	Expressed at low levels throughout the embryo. At later developmental stages, expressed in areas of high cell proliferation. From embryo stage 13, expression is high in central nervous system and midgut, especially in the gastric caeca. Expression also seen in larval imaginal disks and brain. Ref.1 Ref.2 Ref.6
Developmental stage	Throughout development, from embryo to adult. Ref.2
Sequence similarities	Belongs to the selenophosphate synthase 1 family. Class II subfamily.
Caution	The conserved active site Cys (or selenocysteine) residue in position 49 is replaced by an Arg. However, as function in selenoprotein synthesis is proven, it is possible Cys-51 is the active site.

Ontologies

Keywords
Biological process	Cell cycle
Ligand	ATP-binding Nucleotide-binding Selenium
Molecular function	Developmental protein Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell proliferation Inferred from mutant phenotype Ref.6. Source: FlyBase glutamine metabolic process Inferred from mutant phenotype. Source: FlyBase imaginal disc development Inferred from mutant phenotype Ref.2. Source: FlyBase mitochondrion organization Inferred from mutant phenotype. Source: FlyBase negative regulation of reactive oxygen species metabolic process Inferred from mutant phenotype. Source: FlyBase positive regulation of cell proliferation Inferred from mutant phenotype. Source: FlyBase selenocysteine biosynthetic process Inferred from mutant phenotype Ref.6. Source: FlyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW selenide, water dikinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CG14667	Q8MYW5	1	EBI-156940,EBI-186621
Mical	Q8MTA1	1	EBI-156940,EBI-107489
Sry-beta	P07665	1	EBI-156940,EBI-162141

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 398	398	Selenide, water dikinase		PRO_0000127652
Regions
	Nucleotide binding	290 – 296	7	ATP Potential
Sites
	Active site	51	1	Potential
	Site	52	1	Important for catalytic activity By similarity
Experimental info
	Sequence conflict	253 – 254	2	ED → KN in AAB88790. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O18373 [UniParc]. Last modified January 1, 1998. Version 1. Checksum: B265DAF4FDACC2D8	FASTA	398	43,446
	10 20 30 40 50 60 MSYAADVLNS AHLELHGGGD AELRRPFDPT AHDLDASFRL TRFADLKGRG CKVPQDVLSK 70 80 90 100 110 120 LVSALQQDYS AQDQEPQFLN VAIPRIGIGL DCSVIPLRHG GLCLVQTTDF FYPIVDDPYM 130 140 150 160 170 180 MGKIACANVL SDLYAMGVTD CDNMLMLLAV STKMTEKERD VVIPLIMRGF KDSALEAGTT 190 200 210 220 230 240 VTGGQSVVNP WCTIGGVAST ICQPNEYIVP DNAVVGDVLV LTKPLGTQVA VNAHQWIDQP 250 260 270 280 290 300 ERWNRIKLVV SEEDVRKAYH RAMNSMARLN RVAARLMHKY NAHGATDITG FGLLGHAQTL 310 320 330 340 350 360 AAHQKKDVSF VIHNLPVIAK MAAVAKACGN MFQLLQGHSA ETSGGLLICL PREQAAAYCK 370 380 390 DIEKQEGYQA WIIGIVEKGN KTARIIDKPR VIEVPAKD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SelD homolog from Drosophila lacking selenide-dependent monoselenophosphate synthetase activity." Persson B.C., Boeck A., Jaeckle H., Vorbrueggen G. J. Mol. Biol. 274:174-180(1997) [PubMed: 9398525] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Strain: Canton-S. Tissue: Embryo.
[2]	"patufet, the gene encoding the Drosophila melanogaster homologue of selenophosphate synthetase, is involved in imaginal disc morphogenesis." Alsina B., Serras F., Baguna J., Corominas M. Mol. Gen. Genet. 257:113-123(1998) [PubMed: 9491069] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[6]	"Disruption of selenoprotein biosynthesis affects cell proliferation in the imaginal discs and brain of Drosophila melanogaster." Alsina B., Corominas M., Berry M.J., Baguna J., Serras F. J. Cell Sci. 112:2875-2884(1999) [PubMed: 10444382] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY.
[7]	"The class 2 selenophosphate synthetase gene of Drosophila contains a functional mammalian-type SECIS." Hirosawa-Takamori M., Jackle H., Vorbruggen G. EMBO Rep. 1:441-446(2000) [PubMed: 11258485] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ000672 mRNA. Translation: CAA04229.1. U91994 Genomic DNA. Translation: AAB88790.1. AE013599 Genomic DNA. Translation: AAF58266.1. AY095058 mRNA. Translation: AAM11386.1.
RefSeq	NP_725374.1. NM_166045.1. NP_725375.1. NM_166046.1.
UniGene	Dm.2979.
3D structure databases
ProteinModelPortal	O18373.
SMR	O18373. Positions 27-394.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-21887N.
IntAct	O18373. 3 interactions.
MINT	MINT-898227.
STRING	O18373.
Proteomic databases
PRIDE	O18373.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0087564; FBpp0086690; FBgn0261270. FBtr0087565; FBpp0086691; FBgn0261270.
GeneID	36587.
KEGG	dme:Dmel_CG8553.
Organism-specific databases
CTD	36587.
FlyBase	FBgn0261270. SelD.
Phylogenomic databases
eggNOG	inNOG06151.
GeneTree	EMGT00050000007241.
InParanoid	O18373.
OMA	GMDSCVI.
OrthoDB	EOG4GHX4H.
PhylomeDB	O18373.
Gene expression databases
Bgee	O18373.
GermOnline	CG8553. Drosophila melanogaster.
Family and domain databases
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR016188. PurM_N-like. IPR004536. SelD. [Graphical view]
KO	K01008.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
PIRSF	PIRSF036407. Selenphspht_syn. 1 hit.
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00476. SelD. 1 hit.
ProtoNet	Search...
Other
NextBio	799359.

Entry information

Entry name

SPS1_DROME

Accession

Primary (citable) accession number: O18373
Secondary accession number(s): O18597, Q0E979, Q9V700

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	January 1, 1998
Last modified:	January 25, 2012
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project