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Protein

Alpha-amylase 2

Gene

Amy58

Organism
Drosophila ananassae (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
  • chlorideBy similarityNote: Binds 1 Cl- ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi116 – 1161CalciumBy similarity
Metal bindingi165 – 1651Calcium; via carbonyl oxygenBy similarity
Metal bindingi174 – 1741CalciumBy similarity
Binding sitei202 – 2021ChlorideBy similarity
Active sitei204 – 2041NucleophileBy similarity
Metal bindingi208 – 2081Calcium; via carbonyl oxygenBy similarity
Active sitei241 – 2411Proton donorBy similarity
Binding sitei304 – 3041ChlorideBy similarity
Sitei306 – 3061Transition state stabilizerBy similarity
Binding sitei343 – 3431ChlorideBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Chloride, Metal-binding

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase 2 (EC:3.2.1.1By similarity)
Gene namesi
Name:Amy58
Synonyms:Amy2
ORF Names:GF18843
OrganismiDrosophila ananassae (Fruit fly)
Taxonomic identifieri7217 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000007801 Componenti: Unassembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0261676. Dana\Amy58.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1818Sequence analysisAdd
BLAST
Chaini19 – 494476Alpha-amylase 2PRO_0000001361Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi46 ↔ 102By similarity
Disulfide bondi153 ↔ 167By similarity
Disulfide bondi376 ↔ 382By similarity
Disulfide bondi448 ↔ 460By similarity

Keywords - PTMi

Disulfide bond

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi7217.FBpp0122035.

Structurei

3D structure databases

ProteinModelPortaliO18345.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2212. Eukaryota.
COG0366. LUCA.
InParanoidiO18345.
KOiK01176.
OMAiIVELMNH.
OrthoDBiEOG091G07V6.
PhylomeDBiO18345.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O18345-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLAKSIVCL ALLAVANAQF NTNYASGRSG MVHLFEWKWD DIAAECENFL
60 70 80 90 100
GPYGYAGVQV SPVNENAVKD SRPWWERYQP ISYKLVTRSG NEEQFASMVR
110 120 130 140 150
RCNNVGVRIY VDVVFNHMAA DGGTYGTGGS TASPSSKSYP GVPFSSLDFN
160 170 180 190 200
PTCAISNYND ANQVRNCELV GLRDLNQGNS YVQEKIVEFL NHLIDLGVAG
210 220 230 240 250
FRVDAAKHMW PADLGVIYGS LKNLNTDHGF ESGAKAYIVQ EVIDMGGEAI
260 270 280 290 300
SKSEYTGLGA ITEFRHSDSI GKAFRGKNQL QYLVNWGVSW GFAASDRSLV
310 320 330 340 350
FVDNHDNQRG HGAGGADVLT YKVPKQYKMA SAFMLAHPFG TPRVMSSFSF
360 370 380 390 400
TDTDQGPPTT DGQNIASPSF NSDNSCSGGW VCEHRWKQIY NMVGFRNAVG
410 420 430 440 450
SDAIQNWWDN GSNQIAFSRG SKGFVAFNND NYDLNSSVQT GLPAGTYCDV
460 470 480 490
ISGSKSGSSC TGKTVTVGSD GRANISIGSS EDDGVLAIHV NAKL
Length:494
Mass (Da):53,576
Last modified:January 11, 2011 - v2
Checksum:i3886599639E9126C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti25 – 251A → P in AAC79122 (PubMed:9618501).Curated
Sequence conflicti63 – 631V → A in AAC79122 (PubMed:9618501).Curated
Sequence conflicti128 – 1281G → A in AAC79122 (PubMed:9618501).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53698 Genomic DNA. Translation: AAC79122.1.
CH902617 Genomic DNA. Translation: EDV44149.1.
AF238958 Genomic DNA. Translation: AAG45312.1.
AF238959 Genomic DNA. Translation: AAG45313.1.
AF238960 Genomic DNA. Translation: AAG45314.1.
AF238961 Genomic DNA. Translation: AAG45315.1.
AF238962 Genomic DNA. Translation: AAG45316.1.
AF238963 Genomic DNA. Translation: AAG45317.1.
AF238964 Genomic DNA. Translation: AAG45318.1.
AF238965 Genomic DNA. Translation: AAG45319.1.
AF238966 Genomic DNA. Translation: AAG45320.1.
AF238967 Genomic DNA. Translation: AAG45321.1.
AF238968 Genomic DNA. Translation: AAG45322.1.
AF238969 Genomic DNA. Translation: AAG45323.1.
AF238970 Genomic DNA. Translation: AAG45324.1.
AF238971 Genomic DNA. Translation: AAG45325.1.
AF238972 Genomic DNA. Translation: AAG45326.1.
AF238973 Genomic DNA. Translation: AAG45327.1.
AF238974 Genomic DNA. Translation: AAG45328.1.
AF238975 Genomic DNA. Translation: AAG45329.1.
AF238976 Genomic DNA. Translation: AAG45330.1.
AF238977 Genomic DNA. Translation: AAG45331.1.
RefSeqiXP_001955588.1. XM_001955552.2.

Genome annotation databases

EnsemblMetazoaiFBtr0123543; FBpp0122035; FBgn0261676.
GeneIDi6501610.
KEGGidan:Dana_GF18843.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U53698 Genomic DNA. Translation: AAC79122.1.
CH902617 Genomic DNA. Translation: EDV44149.1.
AF238958 Genomic DNA. Translation: AAG45312.1.
AF238959 Genomic DNA. Translation: AAG45313.1.
AF238960 Genomic DNA. Translation: AAG45314.1.
AF238961 Genomic DNA. Translation: AAG45315.1.
AF238962 Genomic DNA. Translation: AAG45316.1.
AF238963 Genomic DNA. Translation: AAG45317.1.
AF238964 Genomic DNA. Translation: AAG45318.1.
AF238965 Genomic DNA. Translation: AAG45319.1.
AF238966 Genomic DNA. Translation: AAG45320.1.
AF238967 Genomic DNA. Translation: AAG45321.1.
AF238968 Genomic DNA. Translation: AAG45322.1.
AF238969 Genomic DNA. Translation: AAG45323.1.
AF238970 Genomic DNA. Translation: AAG45324.1.
AF238971 Genomic DNA. Translation: AAG45325.1.
AF238972 Genomic DNA. Translation: AAG45326.1.
AF238973 Genomic DNA. Translation: AAG45327.1.
AF238974 Genomic DNA. Translation: AAG45328.1.
AF238975 Genomic DNA. Translation: AAG45329.1.
AF238976 Genomic DNA. Translation: AAG45330.1.
AF238977 Genomic DNA. Translation: AAG45331.1.
RefSeqiXP_001955588.1. XM_001955552.2.

3D structure databases

ProteinModelPortaliO18345.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7217.FBpp0122035.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0123543; FBpp0122035; FBgn0261676.
GeneIDi6501610.
KEGGidan:Dana_GF18843.

Organism-specific databases

FlyBaseiFBgn0261676. Dana\Amy58.

Phylogenomic databases

eggNOGiKOG2212. Eukaryota.
COG0366. LUCA.
InParanoidiO18345.
KOiK01176.
OMAiIVELMNH.
OrthoDBiEOG091G07V6.
PhylomeDBiO18345.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMY2_DROAN
AccessioniPrimary (citable) accession number: O18345
Secondary accession number(s): B3LZH0, Q9GN73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: January 11, 2011
Last modified: September 7, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.