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Protein

Alpha-amylase-related protein

Gene

Amyrel

Organism
Drosophila ananassae (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity
  • chlorideBy similarityNote: Binds 1 Cl- ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi117CalciumBy similarity1
Metal bindingi168Calcium; via carbonyl oxygenBy similarity1
Metal bindingi177CalciumBy similarity1
Binding sitei205ChlorideBy similarity1
Active sitei207NucleophileBy similarity1
Metal bindingi211Calcium; via carbonyl oxygenBy similarity1
Active sitei244Proton donorBy similarity1
Binding sitei307ChlorideBy similarity1
Sitei309Transition state stabilizerBy similarity1
Binding sitei342ChlorideBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Calcium, Chloride, Metal-binding

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylase-related protein (EC:3.2.1.1By similarity)
Alternative name(s):
Amy c6
Gene namesi
Name:Amyrel
Synonyms:Amyc6
OrganismiDrosophila ananassae (Fruit fly)
Taxonomic identifieri7217 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora

Organism-specific databases

FlyBaseiFBgn0020487. Dana\Amyc6.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19By similarityAdd BLAST19
ChainiPRO_000000136820 – 493Alpha-amylase-related proteinAdd BLAST474

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei20Pyrrolidone carboxylic acidBy similarity1
Disulfide bondi47 ↔ 103By similarity
Disulfide bondi156 ↔ 170By similarity
Disulfide bondi375 ↔ 381By similarity
Disulfide bondi417 ↔ 440Sequence analysis
Disulfide bondi447 ↔ 459By similarity

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi7217.FBpp0116650.

Structurei

3D structure databases

ProteinModelPortaliO18344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG2212. Eukaryota.
COG0366. LUCA.
OrthoDBiEOG091G07V6.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O18344-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKFATAVIL CLAASSTLAQ HNPHWWGNRN TIVHLFEWKW SDIAAECENF
60 70 80 90 100
LGPKGFAGVQ VSPVNENIIS AGRPWWERYQ PISYKLITRS GNEQEFADMV
110 120 130 140 150
RRCNDVGVRI YVDVLLNHMS GDFDGIAVGT AGSEAEPSKK SYPGVPYSAQ
160 170 180 190 200
DFHPSCEITD WNDRFQVQQC ELVGLKDLDQ SSEWVRSKLI EFLDHLIELG
210 220 230 240 250
VAGFRVDAAK HMAADDLSYI YSTISDLNTE HGFPHNARPF IFQEVIDHGH
260 270 280 290 300
ETVSREEYNQ LGAVTEFRFS EEIGNAFRGN NALKWLQSWG TGWGFLASGQ
310 320 330 340 350
ALTFVDNHDN QRDMGAVLNY KSPKQYKMAT AFHLAYPYGI SRVMSSFAFD
360 370 380 390 400
DHDTAPPQDE QERIISPEFD EEGACVNGWI CEHRWRQIYA MVGFKNAVRD
410 420 430 440 450
TELSNWWDNG DSQISFCRGN KGFLAVNNNL YDLSQELQTC LPAGVYCDVI
460 470 480 490
CGSLVDGSCT GKSVIVDDSG FGYIHIGSED FDGVLALHVD ARV
Length:493
Mass (Da):55,400
Last modified:January 31, 2002 - v2
Checksum:iAADC543547BC3C79
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF024691 Genomic DNA. Translation: AAC39090.3.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF024691 Genomic DNA. Translation: AAC39090.3.

3D structure databases

ProteinModelPortaliO18344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7217.FBpp0116650.

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

FlyBaseiFBgn0020487. Dana\Amyc6.

Phylogenomic databases

eggNOGiKOG2212. Eukaryota.
COG0366. LUCA.
OrthoDBiEOG091G07V6.

Family and domain databases

Gene3Di2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
InterProiIPR006048. A-amylase/branching_C.
IPR031319. A-amylase_C.
IPR006046. Alpha_amylase.
IPR015902. Glyco_hydro_13.
IPR006047. Glyco_hydro_13_cat_dom.
IPR013780. Glyco_hydro_b.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
[Graphical view]
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiSM00642. Aamy. 1 hit.
SM00632. Aamy_C. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiAMYR_DROAN
AccessioniPrimary (citable) accession number: O18344
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 31, 2002
Last modified: October 5, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.